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The map label for this gene is gidA [H]

Identifier: 197294686

GI number: 197294686

Start: 643520

End: 645364

Strand: Direct

Name: gidA [H]

Synonym: PAa_0644

Alternate gene names: 197294686

Gene position: 643520-645364 (Clockwise)

Preceding gene: 197294685

Following gene: 197294687

Centisome position: 73.13

GC content: 31.54

Gene sequence:

>1845_bases
ATGGAATACGATAGTATTGTTGTTGGCGCAGGACACGCAGGAGTTGAAGCTGCTTTAATATTGGCTAAAAAACATCAAAC
TTTATTAATTTCAGGTTCTTTAAAAAAAATAGCCTCTCTACATTGCAACCCTTCAATCGGAGGACCTGCTAAAGGAGTTG
TTGTTCGTGAAATCGATGCTTTAGGGGGAGTAATGGGGAAAGCAGCAGATTTAGCACAAATACAAATGAAAATGCTTAAC
TTTTCCAAAGGACCCGCCGTTAGAGCTTTAAGAGCTCAAATTGACAAAATACAATACCCCAAAATTATTTTAAAAATGTT
GCAAACCGCTCCTAATTTAGATTTATTAGAAGGGTTAGTAAATCATTTATTAATCAAGAATAATCAAATTCAAGGGGTTT
GTTTGTTAGATGGTCGAAAAATTTATGCAAAAACTGTCATTATTACAACCGGAACTTATTTATCAAGTCAAATATTGATT
GGAGAAACTAAAACAGCCTCTGGACCCAACGGAACTCCTACCACTTATGGCATCAGCCAACAATTAAAAGATTTAGGGTT
TGAAATTATTCGCCTTAAAACGGGAACGCCGCCAAGAATTAAAAAAGCAACGATTGATTACGCTCAAACCAAAATCCAAT
TAGGCGATACAGATTTACAAACATTTAGTTTTGATAGTGATATAAAAAAATTAGGATCACAAGAAGCTTGTTTTTTAACA
CATACTAACGAAAAAACCCATCAAGTAATTCAAAAGCATCTCAACAAATCAGCAATGTATGGCGGATATATTAAAGGAAC
GGGGCCACGATATTGCCCTTCTATTGAAGATAAAGTAGTAAGGTTTTTTGATAAAAAAAGTCATCAAATTTTTATTGAAC
CTGAAAGTGTTTCTTTAGATGAAATGTATTTGCAAGGACTTTCCACCAGTATGCCGCAAAACATACAACATGAAATTTTA
AAAACTATTCCAGGATTGCAAAACGCTGAAATTGCTAAATATGGTTATGCCATTGAATACGATGCTTTTAACCCAAGCCA
ACTAAAACACAACTTAGAAACTAAAAAAATTAATAATCTTTTTTTTGCAGGACAAATGAATGGCACTAGTGGATATGAAG
AGGCGGCTTGTCAAGGGTTGATGGCAGGAATTAACGCTTCATTGAAACTACAAAATAAAAGTCCTTTTGTTTTAAAAAGA
AACGAAGCTTATATTGGAGTTTTAATTGATGATTTAATCACCAAAGGGACTAAAGAACCTTATCGTTTGTTAACATCTCG
CGCTGAATTCCGTTTATTGTTAAGACATGATAATGCTGATTTGCGTTTAAAAGAATATAGCAATCATTTTGGTTTAATAG
ATGCAAAAAATTATGAAGCTTTTAAAAAGAAAAAAGAATCCCTTCAATTAATTTTAAAAATGAGTCAAAATCATGAAATT
TTGTCTGAAGAAAAAAATTTAGATTATTTAAAAACTACTTTGACAGGGAAAACAACTTTATTTCAATTATTAAAACGTCC
TGAATTCAACTATCAAACATTACAACATTTTTTAAAAATAAAAGCAGATCAAAAAACATATGAACAAGCAGAAATACAGA
TAAAATACGAAGGTTATATTGCAAAAGCAAAAAAAGAAGCAGAGAAGTTATTAAAATTGGAGCAGAAAAAAATCCCTGAA
AAAATCAATTATTCAGAAATTAAAAACTTATCTAAAGAGGCGCAGGAAAAATTAACTTTAATTAAACCAGAAACTTTAGG
CCAGGCTTCAAGAATTTTAGGAGTCAATCAAGTAGATATTTCAATCTTGTTAATTTATTTAGGTAAAGAACGTGCTTTAT
CCTGA

Upstream 100 bases:

>100_bases
ATTGAACAACAAAAAAAATATAATCCTGAATATAAACCATTAGAAAATGAAGCAGAAAAGGATGATAACTTAAAAAATTA
AAAAGGGGAAGAGGTTAAAA

Downstream 100 bases:

>100_bases
TTTATTAACAAAAAAATTCAAACTTAATCCTCATCAATTGCAACAATTTGATTTATACTATCGCTTTTTAAACCAAGAAA
ATAAAAAATATAATCTCACT

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MEYDSIVVGAGHAGVEAALILAKKHQTLLISGSLKKIASLHCNPSIGGPAKGVVVREIDALGGVMGKAADLAQIQMKMLN
FSKGPAVRALRAQIDKIQYPKIILKMLQTAPNLDLLEGLVNHLLIKNNQIQGVCLLDGRKIYAKTVIITTGTYLSSQILI
GETKTASGPNGTPTTYGISQQLKDLGFEIIRLKTGTPPRIKKATIDYAQTKIQLGDTDLQTFSFDSDIKKLGSQEACFLT
HTNEKTHQVIQKHLNKSAMYGGYIKGTGPRYCPSIEDKVVRFFDKKSHQIFIEPESVSLDEMYLQGLSTSMPQNIQHEIL
KTIPGLQNAEIAKYGYAIEYDAFNPSQLKHNLETKKINNLFFAGQMNGTSGYEEAACQGLMAGINASLKLQNKSPFVLKR
NEAYIGVLIDDLITKGTKEPYRLLTSRAEFRLLLRHDNADLRLKEYSNHFGLIDAKNYEAFKKKKESLQLILKMSQNHEI
LSEEKNLDYLKTTLTGKTTLFQLLKRPEFNYQTLQHFLKIKADQKTYEQAEIQIKYEGYIAKAKKEAEKLLKLEQKKIPE
KINYSEIKNLSKEAQEKLTLIKPETLGQASRILGVNQVDISILLIYLGKERALS

Sequences:

>Translated_614_residues
MEYDSIVVGAGHAGVEAALILAKKHQTLLISGSLKKIASLHCNPSIGGPAKGVVVREIDALGGVMGKAADLAQIQMKMLN
FSKGPAVRALRAQIDKIQYPKIILKMLQTAPNLDLLEGLVNHLLIKNNQIQGVCLLDGRKIYAKTVIITTGTYLSSQILI
GETKTASGPNGTPTTYGISQQLKDLGFEIIRLKTGTPPRIKKATIDYAQTKIQLGDTDLQTFSFDSDIKKLGSQEACFLT
HTNEKTHQVIQKHLNKSAMYGGYIKGTGPRYCPSIEDKVVRFFDKKSHQIFIEPESVSLDEMYLQGLSTSMPQNIQHEIL
KTIPGLQNAEIAKYGYAIEYDAFNPSQLKHNLETKKINNLFFAGQMNGTSGYEEAACQGLMAGINASLKLQNKSPFVLKR
NEAYIGVLIDDLITKGTKEPYRLLTSRAEFRLLLRHDNADLRLKEYSNHFGLIDAKNYEAFKKKKESLQLILKMSQNHEI
LSEEKNLDYLKTTLTGKTTLFQLLKRPEFNYQTLQHFLKIKADQKTYEQAEIQIKYEGYIAKAKKEAEKLLKLEQKKIPE
KINYSEIKNLSKEAQEKLTLIKPETLGQASRILGVNQVDISILLIYLGKERALS
>Mature_614_residues
MEYDSIVVGAGHAGVEAALILAKKHQTLLISGSLKKIASLHCNPSIGGPAKGVVVREIDALGGVMGKAADLAQIQMKMLN
FSKGPAVRALRAQIDKIQYPKIILKMLQTAPNLDLLEGLVNHLLIKNNQIQGVCLLDGRKIYAKTVIITTGTYLSSQILI
GETKTASGPNGTPTTYGISQQLKDLGFEIIRLKTGTPPRIKKATIDYAQTKIQLGDTDLQTFSFDSDIKKLGSQEACFLT
HTNEKTHQVIQKHLNKSAMYGGYIKGTGPRYCPSIEDKVVRFFDKKSHQIFIEPESVSLDEMYLQGLSTSMPQNIQHEIL
KTIPGLQNAEIAKYGYAIEYDAFNPSQLKHNLETKKINNLFFAGQMNGTSGYEEAACQGLMAGINASLKLQNKSPFVLKR
NEAYIGVLIDDLITKGTKEPYRLLTSRAEFRLLLRHDNADLRLKEYSNHFGLIDAKNYEAFKKKKESLQLILKMSQNHEI
LSEEKNLDYLKTTLTGKTTLFQLLKRPEFNYQTLQHFLKIKADQKTYEQAEIQIKYEGYIAKAKKEAEKLLKLEQKKIPE
KINYSEIKNLSKEAQEKLTLIKPETLGQASRILGVNQVDISILLIYLGKERALS

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=631, Percent_Identity=39.6196513470681, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI19882217, Length=656, Percent_Identity=38.109756097561, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI183227703, Length=671, Percent_Identity=37.4068554396423, Blast_Score=399, Evalue=1e-111,
Organism=Escherichia coli, GI2367273, Length=619, Percent_Identity=46.0420032310178, Blast_Score=563, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI17534255, Length=615, Percent_Identity=36.5853658536585, Blast_Score=394, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321202, Length=615, Percent_Identity=41.6260162601626, Blast_Score=450, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24658174, Length=631, Percent_Identity=38.1933438985737, Blast_Score=413, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 68721; Mature: 68721

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYDSIVVGAGHAGVEAALILAKKHQTLLISGSLKKIASLHCNPSIGGPAKGVVVREIDA
CCCCCEEEECCCCCCCEEEEEEECCCEEEEECCHHHHHHCCCCCCCCCCCCCEEEEEHHH
LGGVMGKAADLAQIQMKMLNFSKGPAVRALRAQIDKIQYPKIILKMLQTAPNLDLLEGLV
HHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHH
NHLLIKNNQIQGVCLLDGRKIYAKTVIITTGTYLSSQILIGETKTASGPNGTPTTYGISQ
HHHEECCCCCCEEEEECCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHH
QLKDLGFEIIRLKTGTPPRIKKATIDYAQTKIQLGDTDLQTFSFDSDIKKLGSQEACFLT
HHHHCCEEEEEEECCCCCCCCHHHHHHHHEEEEECCCCCEEEECCHHHHHCCCCCEEEEE
HTNEKTHQVIQKHLNKSAMYGGYIKGTGPRYCPSIEDKVVRFFDKKSHQIFIEPESVSLD
ECCHHHHHHHHHHCCCCHHCCCEEECCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCHH
EMYLQGLSTSMPQNIQHEILKTIPGLQNAEIAKYGYAIEYDAFNPSQLKHNLETKKINNL
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHCCEEEEECCCCHHHHHHCCCHHHHCCE
FFAGQMNGTSGYEEAACQGLMAGINASLKLQNKSPFVLKRNEAYIGVLIDDLITKGTKEP
EEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCEEEEEHHHHHHCCCCCH
YRLLTSRAEFRLLLRHDNADLRLKEYSNHFGLIDAKNYEAFKKKKESLQLILKMSQNHEI
HHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCH
LSEEKNLDYLKTTLTGKTTLFQLLKRPEFNYQTLQHFLKIKADQKTYEQAEIQIKYEGYI
HHHCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCH
AKAKKEAEKLLKLEQKKIPEKINYSEIKNLSKEAQEKLTLIKPETLGQASRILGVNQVDI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEECCHHHCCHHHHCCCCEECE
SILLIYLGKERALS
EEEEEEECCHHCCC
>Mature Secondary Structure
MEYDSIVVGAGHAGVEAALILAKKHQTLLISGSLKKIASLHCNPSIGGPAKGVVVREIDA
CCCCCEEEECCCCCCCEEEEEEECCCEEEEECCHHHHHHCCCCCCCCCCCCCEEEEEHHH
LGGVMGKAADLAQIQMKMLNFSKGPAVRALRAQIDKIQYPKIILKMLQTAPNLDLLEGLV
HHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHH
NHLLIKNNQIQGVCLLDGRKIYAKTVIITTGTYLSSQILIGETKTASGPNGTPTTYGISQ
HHHEECCCCCCEEEEECCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCHHH
QLKDLGFEIIRLKTGTPPRIKKATIDYAQTKIQLGDTDLQTFSFDSDIKKLGSQEACFLT
HHHHCCEEEEEEECCCCCCCCHHHHHHHHEEEEECCCCCEEEECCHHHHHCCCCCEEEEE
HTNEKTHQVIQKHLNKSAMYGGYIKGTGPRYCPSIEDKVVRFFDKKSHQIFIEPESVSLD
ECCHHHHHHHHHHCCCCHHCCCEEECCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCHH
EMYLQGLSTSMPQNIQHEILKTIPGLQNAEIAKYGYAIEYDAFNPSQLKHNLETKKINNL
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHCCEEEEECCCCHHHHHHCCCHHHHCCE
FFAGQMNGTSGYEEAACQGLMAGINASLKLQNKSPFVLKRNEAYIGVLIDDLITKGTKEP
EEEEECCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEEECCCCEEEEEHHHHHHCCCCCH
YRLLTSRAEFRLLLRHDNADLRLKEYSNHFGLIDAKNYEAFKKKKESLQLILKMSQNHEI
HHHHHCCCCEEEEEEECCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCH
LSEEKNLDYLKTTLTGKTTLFQLLKRPEFNYQTLQHFLKIKADQKTYEQAEIQIKYEGYI
HHHCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEECCCH
AKAKKEAEKLLKLEQKKIPEKINYSEIKNLSKEAQEKLTLIKPETLGQASRILGVNQVDI
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEECCHHHCCHHHHCCCCEECE
SILLIYLGKERALS
EEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA