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The map label for this gene is smpB [H]

Identifier: 197294647

GI number: 197294647

Start: 596833

End: 597363

Strand: Direct

Name: smpB [H]

Synonym: PAa_0603

Alternate gene names: 197294647

Gene position: 596833-597363 (Clockwise)

Preceding gene: 197294646

Following gene: 197294648

Centisome position: 67.83

GC content: 21.85

Gene sequence:

>531_bases
GTGTTGAAGAACCAATTTTTTGGTTGTTTTTTCTTAATCTCTCTTTTCAATTATTGTAAGAAAGTATGTTTTATAATTAT
GAAAACAATAGCAACCAATAAAAAAGCAGTTTTTGATTTTTTTTTAGAAAAGAAAAAATATCATGCAGGCATAAAACTTT
TAGGCAACGAAGTAAAATCAATTAGATTAGGTAAAATAAGCCTAAATAACTCTTATGCTTATATTAAAAATAACGAAATT
TTTATAGTTAACTTGTCTATTTTAAAATACCCATTTAGTTGTTGTATGCAGTATGATGAAAATAGAACTAAAAAACTTTT
ATTACGAAAGCAAGAAATTATTCAAATTCAAAACAAAATTAAAACCCAAGGTTTTTCAGTTATTCCTTTAAAAGTCTATT
TTGAAAAAAATTTAGTTAAAGTAGAAATTGTTTTAGCTAAAGGTAAAAAAAAATATGACAAAAGAAATAGTTTAAAAGAA
AAAGCAGCTCAATTATCCATTCAAAAAAATTTAAAAAAAATCAATTATTAA

Upstream 100 bases:

>100_bases
TATCGCTTATAGAAAATTAAAAAAAGATTAATTATTATGAAGAAGAAAGAGTTAAATATTTTGGTTTTTTGTTTAAGATT
TTAAAATTTCAAACTCATTC

Downstream 100 bases:

>100_bases
AATTATCGCACCATTAACTGTTTTCAAAGTTTAGACAAACTTTTGTTTTGGTTTTTTCTTTTAAAATTAAAAAGATTGCA
ATAAATTCAGATTTATTCAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MLKNQFFGCFFLISLFNYCKKVCFIIMKTIATNKKAVFDFFLEKKKYHAGIKLLGNEVKSIRLGKISLNNSYAYIKNNEI
FIVNLSILKYPFSCCMQYDENRTKKLLLRKQEIIQIQNKIKTQGFSVIPLKVYFEKNLVKVEIVLAKGKKKYDKRNSLKE
KAAQLSIQKNLKKINY

Sequences:

>Translated_176_residues
MLKNQFFGCFFLISLFNYCKKVCFIIMKTIATNKKAVFDFFLEKKKYHAGIKLLGNEVKSIRLGKISLNNSYAYIKNNEI
FIVNLSILKYPFSCCMQYDENRTKKLLLRKQEIIQIQNKIKTQGFSVIPLKVYFEKNLVKVEIVLAKGKKKYDKRNSLKE
KAAQLSIQKNLKKINY
>Mature_176_residues
MLKNQFFGCFFLISLFNYCKKVCFIIMKTIATNKKAVFDFFLEKKKYHAGIKLLGNEVKSIRLGKISLNNSYAYIKNNEI
FIVNLSILKYPFSCCMQYDENRTKKLLLRKQEIIQIQNKIKTQGFSVIPLKVYFEKNLVKVEIVLAKGKKKYDKRNSLKE
KAAQLSIQKNLKKINY

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=141, Percent_Identity=36.1702127659575, Blast_Score=102, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 20702; Mature: 20702

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNQFFGCFFLISLFNYCKKVCFIIMKTIATNKKAVFDFFLEKKKYHAGIKLLGNEVKS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHCE
IRLGKISLNNSYAYIKNNEIFIVNLSILKYPFSCCMQYDENRTKKLLLRKQEIIQIQNKI
EEEEEEEECCCEEEEECCEEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KTQGFSVIPLKVYFEKNLVKVEIVLAKGKKKYDKRNSLKEKAAQLSIQKNLKKINY
HHCCCEEEEEEEEEECCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKNQFFGCFFLISLFNYCKKVCFIIMKTIATNKKAVFDFFLEKKKYHAGIKLLGNEVKS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHCE
IRLGKISLNNSYAYIKNNEIFIVNLSILKYPFSCCMQYDENRTKKLLLRKQEIIQIQNKI
EEEEEEEECCCEEEEECCEEEEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
KTQGFSVIPLKVYFEKNLVKVEIVLAKGKKKYDKRNSLKEKAAQLSIQKNLKKINY
HHCCCEEEEEEEEEECCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA