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The map label for this gene is potD [H]

Identifier: 197294269

GI number: 197294269

Start: 152524

End: 153567

Strand: Reverse

Name: potD [H]

Synonym: PAa_0132

Alternate gene names: 197294269

Gene position: 153567-152524 (Counterclockwise)

Preceding gene: 197294270

Following gene: 197294268

Centisome position: 17.45

GC content: 26.34

Gene sequence:

>1044_bases
TTGTTTAATTGGGGTGAGTATTTAGATCCTCAAACCATTATTGATTTTGAAGAACAAAATGGAATTGAAGTTAAACAAGT
TCTTTTTTCTTCTAACGAACTAGCGATTACAAAAATTAAAAGTGATGATAAATATGATTTGGCCATTTTAAGTGAATATG
CAATTGATAAATTAATCCAAGACGAATTAATAGAAGGGATCAATTCTGAAAAATTAGAAAAGAGTAATAACTATAATTGG
CAATATAATGACCAATATAAAGAATTCAAAGAAAAATTACCGAAAAGATTTTCTAATTATGCCGTTCCTTATTTTTGGGG
CAAAGTAGTTCTTATTTATAACAAACAAAAAATTAATAAAGAAGAAATAACAAAAGAGAGTTTTAAAATTTTGGATAAAC
CCAATTTAAAAATTGCTTTATGCAATAATCCACGAGATGGATTAATGGTAGGCCTCAAAGCTACCGGGGGAACAATTGAA
AAACCCACAGAAAAAGAATTAGAAAATGCTAAAAAATGGTTATTAAAACTTAAAGAAAAAAATCCTAATGTTGCCTTTAT
TAATGACCAACTTATTGATCGAATGTCGCAAAAAGGTAAAGAATATTATGATGTGGTAGTGGCTTATTCCGGAGACGCTA
ATTTTTTAAAAGAAAAAAACGATAATTTAGAAGTTTTAGATCCTAAAGAAGGTACTAATGTTTGGGTGGATAGTTTAGTG
ATGCCTAAAAATTCTTGTAAAGAATTAGCTTATAAATTTATTAATTTTTTAAGAGAAAAAAATAACTATCAAAAAAACTT
GGCATATATTAAATATGATTCTCCTTACAAAGACAAAAACAACAAGATTAAAATTAACGAAAATGATCAAATTTATAAAT
ATGATGAAGCTAATCAAAAATTAATTAATACTTACTGGAATGACGTTATTGCTTATCCGAGCAAAAAAGATTATTGGCTT
TTTGGCTTTAGTGGATTGATTTTGATTGGTTTAATAATTGCGTTTTTTATGAAAAAAAGAAAAGTTTTTGAATCACAATA
CTAA

Upstream 100 bases:

>100_bases
TTGCACGCTTTTTTTAACAATCTTTGTAATGATTTTAATATTTATACAAAATAGGACCAAAAAAAGTGCAATAACTAAAG
AATCCAAGCAATTTTTGACC

Downstream 100 bases:

>100_bases
ACAATACAAAAAAATAATAATTACATGAGTTTTTATGAGGAAGAATATCGTTATTTCTAAAGAAAAAACAAGCCCTTATT
TGCAAAAAAATAATTTTTGA

Product: ABC-type spermidine/putrescine-binding periplasmic protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MFNWGEYLDPQTIIDFEEQNGIEVKQVLFSSNELAITKIKSDDKYDLAILSEYAIDKLIQDELIEGINSEKLEKSNNYNW
QYNDQYKEFKEKLPKRFSNYAVPYFWGKVVLIYNKQKINKEEITKESFKILDKPNLKIALCNNPRDGLMVGLKATGGTIE
KPTEKELENAKKWLLKLKEKNPNVAFINDQLIDRMSQKGKEYYDVVVAYSGDANFLKEKNDNLEVLDPKEGTNVWVDSLV
MPKNSCKELAYKFINFLREKNNYQKNLAYIKYDSPYKDKNNKIKINENDQIYKYDEANQKLINTYWNDVIAYPSKKDYWL
FGFSGLILIGLIIAFFMKKRKVFESQY

Sequences:

>Translated_347_residues
MFNWGEYLDPQTIIDFEEQNGIEVKQVLFSSNELAITKIKSDDKYDLAILSEYAIDKLIQDELIEGINSEKLEKSNNYNW
QYNDQYKEFKEKLPKRFSNYAVPYFWGKVVLIYNKQKINKEEITKESFKILDKPNLKIALCNNPRDGLMVGLKATGGTIE
KPTEKELENAKKWLLKLKEKNPNVAFINDQLIDRMSQKGKEYYDVVVAYSGDANFLKEKNDNLEVLDPKEGTNVWVDSLV
MPKNSCKELAYKFINFLREKNNYQKNLAYIKYDSPYKDKNNKIKINENDQIYKYDEANQKLINTYWNDVIAYPSKKDYWL
FGFSGLILIGLIIAFFMKKRKVFESQY
>Mature_347_residues
MFNWGEYLDPQTIIDFEEQNGIEVKQVLFSSNELAITKIKSDDKYDLAILSEYAIDKLIQDELIEGINSEKLEKSNNYNW
QYNDQYKEFKEKLPKRFSNYAVPYFWGKVVLIYNKQKINKEEITKESFKILDKPNLKIALCNNPRDGLMVGLKATGGTIE
KPTEKELENAKKWLLKLKEKNPNVAFINDQLIDRMSQKGKEYYDVVVAYSGDANFLKEKNDNLEVLDPKEGTNVWVDSLV
MPKNSCKELAYKFINFLREKNNYQKNLAYIKYDSPYKDKNNKIKINENDQIYKYDEANQKLINTYWNDVIAYPSKKDYWL
FGFSGLILIGLIIAFFMKKRKVFESQY

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=282, Percent_Identity=29.4326241134752, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787078, Length=332, Percent_Identity=24.6987951807229, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40914; Mature: 40914

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNWGEYLDPQTIIDFEEQNGIEVKQVLFSSNELAITKIKSDDKYDLAILSEYAIDKLIQ
CCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
DELIEGINSEKLEKSNNYNWQYNDQYKEFKEKLPKRFSNYAVPYFWGKVVLIYNKQKINK
HHHHCCCCHHHHHCCCCCCCEECCHHHHHHHHHHHHHHCCCCCEEECEEEEEEECCCCCH
EEITKESFKILDKPNLKIALCNNPRDGLMVGLKATGGTIEKPTEKELENAKKWLLKLKEK
HHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCC
NPNVAFINDQLIDRMSQKGKEYYDVVVAYSGDANFLKEKNDNLEVLDPKEGTNVWVDSLV
CCCEEEECHHHHHHHHHCCCCEEEEEEEECCCCHHHHCCCCCCEEECCCCCCCEEEHEEE
MPKNSCKELAYKFINFLREKNNYQKNLAYIKYDSPYKDKNNKIKINENDQIYKYDEANQK
CCCHHHHHHHHHHHHHHHHCCCCHHCEEEEEECCCCCCCCCEEEECCCCCEEEECCHHHH
LINTYWNDVIAYPSKKDYWLFGFSGLILIGLIIAFFMKKRKVFESQY
HHHHHHHHHEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFNWGEYLDPQTIIDFEEQNGIEVKQVLFSSNELAITKIKSDDKYDLAILSEYAIDKLIQ
CCCCCCCCCCCCEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
DELIEGINSEKLEKSNNYNWQYNDQYKEFKEKLPKRFSNYAVPYFWGKVVLIYNKQKINK
HHHHCCCCHHHHHCCCCCCCEECCHHHHHHHHHHHHHHCCCCCEEECEEEEEEECCCCCH
EEITKESFKILDKPNLKIALCNNPRDGLMVGLKATGGTIEKPTEKELENAKKWLLKLKEK
HHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCC
NPNVAFINDQLIDRMSQKGKEYYDVVVAYSGDANFLKEKNDNLEVLDPKEGTNVWVDSLV
CCCEEEECHHHHHHHHHCCCCEEEEEEEECCCCHHHHCCCCCCEEECCCCCCCEEEHEEE
MPKNSCKELAYKFINFLREKNNYQKNLAYIKYDSPYKDKNNKIKINENDQIYKYDEANQK
CCCHHHHHHHHHHHHHHHHCCCCHHCEEEEEECCCCCCCCCEEEECCCCCEEEECCHHHH
LINTYWNDVIAYPSKKDYWLFGFSGLILIGLIIAFFMKKRKVFESQY
HHHHHHHHHEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]