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The map label for this gene is thrS

Identifier: 197294184

GI number: 197294184

Start: 21039

End: 22964

Strand: Reverse

Name: thrS

Synonym: PAa_0018

Alternate gene names: 197294184

Gene position: 22964-21039 (Counterclockwise)

Preceding gene: 197294185

Following gene: 197294178

Centisome position: 2.61

GC content: 29.44

Gene sequence:

>1926_bases
ATGATTAAAATTAATCTTTTAAATCATCAAAGCCATTCATTTCCTTCCAAAACAACTCCTTTGGAAATTTGGAAAAATTG
GTTAAAAAAAACTTTGAAAAAACCAGTTGCAGCTCTTTTTAACCAAAAATTCATAGAACTTGATTATCCTTTAACACAAG
ATGGAGACTTAGAAATTTTGGCAGAAAGTAATCCCAAATCGTTATTTGTTCTAAACCATAGCACGGCTCATTTAATGGCT
CAAGCAATCCAAAGACTTTATCCAAACGCTCTTTTTACAATTGGACCAGCCATTAAAGAAGGTTTTTATTATGACATTGA
TTTTCAAAACCATTCTATTTCAGAAAAAGATTTGCCAACAATTGAAAAAAAAATGCACGAAGTAGCTTTAGAAAATCACT
CCATGATAATGAAAAAAGTAACGCATGATGAAGCTAAAAGGCTTTTTTCCTATAATCCTTATAAATTAATTTTATTAGAA
AAACACAAAGAAGAAGATATTACTGTTTGTCATCAAGGTGAATTTATTGACCTTTGTCGTGGGGGTCATATTCCTAAATT
ATCTTTAATAAAACATTTTAAGCTTTTAAAAATTTCTGGTTCTTATTTTCAAGGTGATGCTAAAAATAAATCATTAACTC
GTATTTATGGCACTTCTTTTTTTAAAAAAGAAGACCTTGGCAATTATTTGAAACTTTTAGAAGAAAGAAAAGAACGCGAC
CATAAAAGACTTAATAAAAAATTAGATTTATTTATGTTTTCTCAAGAAGTGGGGTTGGGTTTACCTTTTTGGTTGCCTAA
AGGAGCCACTTTGAGGCGTATTGTTGAAAGGTATATTGTTGATAAAGAATTGAGTCATCAATATCATCACGTCTATACTC
CTATTATGGCAAACGTAGAGCTTTATCGCACTTCAGGTCATTTAGAACATTATTCTCAAAACATGTTTCCTGTGATGCAA
TTAGAAAATAAAGAAAAAATAGTTTTAAGACCGATGAACTGTCCACATCATATGATGATTTATAAAAAAAGTCCTCGTAG
TTATAAAGAGTTGCCATTAAGAATTGCTGAATTAGGGATGATGCATCGTTTTGAAAAATCAGGCGCTGTTTCAGGATTAC
AAAGAGTAAGAGAAATGAATCTTAATGATGCTCATAATTTTGTAAGACCTGATCAAATTGAAGAAGAAATCAAAAAAATC
ATCAATTTAATTTTAGAGGTTTATCGTGATTTTAAAATCACAAAATACGAATTCCGTTTAAGTTATCGTGATCCTCAAGA
TAAAGAAAAATATTTTCCAGATGACAATATGTGGCAACATGCAGAAAACATTTTAAAAAAAACAATCCAAGAATTAAATT
TACCTTTTAGAGAAGCCATTGGCGATGCTGCTTTTTATGGCCCTAAGTTAGATGTTCAAGTACTTACTGCTTTAGGAAAT
GAAGAAACTTTATCAACAATTCAATTAGATTTTTTATTACCGCAAAAATTTGATTTAACTTTTATTGATGCTAATAACAA
ACATTGCCGTCCTGTTGTCATTCATCGCGCTATTGTTTCTACTTTGGAACGTTTTTTATCTCATTTAATTGAAGAAAATA
AAGGTGTTTTTCCTTTGTGGCTTGCTCCTGTACAAATACTTTTAATTCCTGTTTCTTCTTCGGTTAATTTAAAATATACT
CAAGAAATTAAAGAACTTTTATTATCTCAAGGATTAAGAGCAGAAATTAATTCCAAAGAGGCAACTTTGGGTTATAAAAT
CAGAGAAGCTCAAGAATTAAAAATCCCTTATCAAATAGTTGTTGGTGATAATGAAATTGCTAAAAATTTGATTACTTTTA
GAAAATATGGCCAAAAAAACCAAACAACTACAAACATTGAAACTTTTATTTCTTCTCTTAATCAAGAAATTATGGAAAAA
AGATAA

Upstream 100 bases:

>100_bases
AAGAAATTGAACCGTTTATAAAATCGCTAGAAAAAGATTTATCTATTTATTTAAAACAACTTAAGTAATATTTATAAAAA
ATTTCAGAAAGGGTTATTTT

Downstream 100 bases:

>100_bases
TCTTAAAATATTTTTTTCACTTAACTTAAATTATTTAATTTTCTCAACGTTATCTTTGGGATTGTTTAAATTTTTAAGTT
TTTCTTTGGATTCAAAGGGT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MIKINLLNHQSHSFPSKTTPLEIWKNWLKKTLKKPVAALFNQKFIELDYPLTQDGDLEILAESNPKSLFVLNHSTAHLMA
QAIQRLYPNALFTIGPAIKEGFYYDIDFQNHSISEKDLPTIEKKMHEVALENHSMIMKKVTHDEAKRLFSYNPYKLILLE
KHKEEDITVCHQGEFIDLCRGGHIPKLSLIKHFKLLKISGSYFQGDAKNKSLTRIYGTSFFKKEDLGNYLKLLEERKERD
HKRLNKKLDLFMFSQEVGLGLPFWLPKGATLRRIVERYIVDKELSHQYHHVYTPIMANVELYRTSGHLEHYSQNMFPVMQ
LENKEKIVLRPMNCPHHMMIYKKSPRSYKELPLRIAELGMMHRFEKSGAVSGLQRVREMNLNDAHNFVRPDQIEEEIKKI
INLILEVYRDFKITKYEFRLSYRDPQDKEKYFPDDNMWQHAENILKKTIQELNLPFREAIGDAAFYGPKLDVQVLTALGN
EETLSTIQLDFLLPQKFDLTFIDANNKHCRPVVIHRAIVSTLERFLSHLIEENKGVFPLWLAPVQILLIPVSSSVNLKYT
QEIKELLLSQGLRAEINSKEATLGYKIREAQELKIPYQIVVGDNEIAKNLITFRKYGQKNQTTTNIETFISSLNQEIMEK
R

Sequences:

>Translated_641_residues
MIKINLLNHQSHSFPSKTTPLEIWKNWLKKTLKKPVAALFNQKFIELDYPLTQDGDLEILAESNPKSLFVLNHSTAHLMA
QAIQRLYPNALFTIGPAIKEGFYYDIDFQNHSISEKDLPTIEKKMHEVALENHSMIMKKVTHDEAKRLFSYNPYKLILLE
KHKEEDITVCHQGEFIDLCRGGHIPKLSLIKHFKLLKISGSYFQGDAKNKSLTRIYGTSFFKKEDLGNYLKLLEERKERD
HKRLNKKLDLFMFSQEVGLGLPFWLPKGATLRRIVERYIVDKELSHQYHHVYTPIMANVELYRTSGHLEHYSQNMFPVMQ
LENKEKIVLRPMNCPHHMMIYKKSPRSYKELPLRIAELGMMHRFEKSGAVSGLQRVREMNLNDAHNFVRPDQIEEEIKKI
INLILEVYRDFKITKYEFRLSYRDPQDKEKYFPDDNMWQHAENILKKTIQELNLPFREAIGDAAFYGPKLDVQVLTALGN
EETLSTIQLDFLLPQKFDLTFIDANNKHCRPVVIHRAIVSTLERFLSHLIEENKGVFPLWLAPVQILLIPVSSSVNLKYT
QEIKELLLSQGLRAEINSKEATLGYKIREAQELKIPYQIVVGDNEIAKNLITFRKYGQKNQTTTNIETFISSLNQEIMEK
R
>Mature_641_residues
MIKINLLNHQSHSFPSKTTPLEIWKNWLKKTLKKPVAALFNQKFIELDYPLTQDGDLEILAESNPKSLFVLNHSTAHLMA
QAIQRLYPNALFTIGPAIKEGFYYDIDFQNHSISEKDLPTIEKKMHEVALENHSMIMKKVTHDEAKRLFSYNPYKLILLE
KHKEEDITVCHQGEFIDLCRGGHIPKLSLIKHFKLLKISGSYFQGDAKNKSLTRIYGTSFFKKEDLGNYLKLLEERKERD
HKRLNKKLDLFMFSQEVGLGLPFWLPKGATLRRIVERYIVDKELSHQYHHVYTPIMANVELYRTSGHLEHYSQNMFPVMQ
LENKEKIVLRPMNCPHHMMIYKKSPRSYKELPLRIAELGMMHRFEKSGAVSGLQRVREMNLNDAHNFVRPDQIEEEIKKI
INLILEVYRDFKITKYEFRLSYRDPQDKEKYFPDDNMWQHAENILKKTIQELNLPFREAIGDAAFYGPKLDVQVLTALGN
EETLSTIQLDFLLPQKFDLTFIDANNKHCRPVVIHRAIVSTLERFLSHLIEENKGVFPLWLAPVQILLIPVSSSVNLKYT
QEIKELLLSQGLRAEINSKEATLGYKIREAQELKIPYQIVVGDNEIAKNLITFRKYGQKNQTTTNIETFISSLNQEIMEK
R

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=623, Percent_Identity=36.2760834670947, Blast_Score=390, Evalue=1e-108,
Organism=Homo sapiens, GI38570111, Length=607, Percent_Identity=35.4200988467875, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI20070344, Length=632, Percent_Identity=33.5443037974684, Blast_Score=350, Evalue=2e-96,
Organism=Escherichia coli, GI1788013, Length=645, Percent_Identity=35.1937984496124, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71984192, Length=637, Percent_Identity=34.3799058084772, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71984184, Length=637, Percent_Identity=34.3799058084772, Blast_Score=375, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6322112, Length=648, Percent_Identity=34.1049382716049, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6322654, Length=394, Percent_Identity=36.2944162436548, Blast_Score=244, Evalue=4e-65,
Organism=Drosophila melanogaster, GI24583842, Length=637, Percent_Identity=34.2229199372057, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI28574141, Length=637, Percent_Identity=34.2229199372057, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24583839, Length=637, Percent_Identity=34.2229199372057, Blast_Score=397, Evalue=1e-110,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_PHYAS (B1V8W6)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001798725.1
- ProteinModelPortal:   B1V8W6
- SMR:   B1V8W6
- GeneID:   6799117
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG352811
- OMA:   GRKWQLG
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 74979; Mature: 74979

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKINLLNHQSHSFPSKTTPLEIWKNWLKKTLKKPVAALFNQKFIELDYPLTQDGDLEIL
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEE
AESNPKSLFVLNHSTAHLMAQAIQRLYPNALFTIGPAIKEGFYYDIDFQNHSISEKDLPT
ECCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHHCCEEEEEECCCCCCCCCCCCH
IEKKMHEVALENHSMIMKKVTHDEAKRLFSYNPYKLILLEKHKEEDITVCHQGEFIDLCR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCHHHHC
GGHIPKLSLIKHFKLLKISGSYFQGDAKNKSLTRIYGTSFFKKEDLGNYLKLLEERKERD
CCCCCHHHHHHHEEEEEECCCEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
HKRLNKKLDLFMFSQEVGLGLPFWLPKGATLRRIVERYIVDKELSHQYHHVYTPIMANVE
HHHHHHHHHHEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCEE
LYRTSGHLEHYSQNMFPVMQLENKEKIVLRPMNCPHHMMIYKKSPRSYKELPLRIAELGM
EEECCCCHHHHHCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHH
MHRFEKSGAVSGLQRVREMNLNDAHNFVRPDQIEEEIKKIINLILEVYRDFKITKYEFRL
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
SYRDPQDKEKYFPDDNMWQHAENILKKTIQELNLPFREAIGDAAFYGPKLDVQVLTALGN
ECCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCHHHHHHCCC
EETLSTIQLDFLLPQKFDLTFIDANNKHCRPVVIHRAIVSTLERFLSHLIEENKGVFPLW
CCHHHEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEH
LAPVQILLIPVSSSVNLKYTQEIKELLLSQGLRAEINSKEATLGYKIREAQELKIPYQIV
HHCEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHCCCCEEEE
VGDNEIAKNLITFRKYGQKNQTTTNIETFISSLNQEIMEKR
ECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKINLLNHQSHSFPSKTTPLEIWKNWLKKTLKKPVAALFNQKFIELDYPLTQDGDLEIL
CEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEE
AESNPKSLFVLNHSTAHLMAQAIQRLYPNALFTIGPAIKEGFYYDIDFQNHSISEKDLPT
ECCCCCEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHHCCEEEEEECCCCCCCCCCCCH
IEKKMHEVALENHSMIMKKVTHDEAKRLFSYNPYKLILLEKHKEEDITVCHQGEFIDLCR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCHHHHC
GGHIPKLSLIKHFKLLKISGSYFQGDAKNKSLTRIYGTSFFKKEDLGNYLKLLEERKERD
CCCCCHHHHHHHEEEEEECCCEEECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHH
HKRLNKKLDLFMFSQEVGLGLPFWLPKGATLRRIVERYIVDKELSHQYHHVYTPIMANVE
HHHHHHHHHHEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCEE
LYRTSGHLEHYSQNMFPVMQLENKEKIVLRPMNCPHHMMIYKKSPRSYKELPLRIAELGM
EEECCCCHHHHHCCCCEEEEECCCCEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHH
MHRFEKSGAVSGLQRVREMNLNDAHNFVRPDQIEEEIKKIINLILEVYRDFKITKYEFRL
HHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEE
SYRDPQDKEKYFPDDNMWQHAENILKKTIQELNLPFREAIGDAAFYGPKLDVQVLTALGN
ECCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHCCCCCCHHHHHHCCC
EETLSTIQLDFLLPQKFDLTFIDANNKHCRPVVIHRAIVSTLERFLSHLIEENKGVFPLW
CCHHHEEEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEH
LAPVQILLIPVSSSVNLKYTQEIKELLLSQGLRAEINSKEATLGYKIREAQELKIPYQIV
HHCEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCHHHCCCCEEEE
VGDNEIAKNLITFRKYGQKNQTTTNIETFISSLNQEIMEKR
ECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA