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The map label for this gene is gltA-2 [H]

Identifier: 197120266

GI number: 197120266

Start: 4442142

End: 4443506

Strand: Direct

Name: gltA-2 [H]

Synonym: Gbem_3905

Alternate gene names: 197120266

Gene position: 4442142-4443506 (Clockwise)

Preceding gene: 197120262

Following gene: 197120268

Centisome position: 96.25

GC content: 59.85

Gene sequence:

>1365_bases
ATGACACAGCTGAAAGACAGGCTAAAGGAGAAGATCGAGGCACACCGTCCCCGCATCGCCCGGCTGACTAAAGAGTTCGG
CTCAGTCATAATCGACAAGGTAGATATAGCGCAGTGCATCGGCGGCGCCCGCGATATCAGGTCCCTTGTTACTGACATCT
CCTATCTCGATCCGCAGGAAGGGATCCGTTTCAGAGGCAAGACCATCCCCGAGACTTTCGAGGCCCTTCCCAAGGCCGCC
GGTTCCGAGTATCCCACAGTGGAATCTTTCTGGTATTTCCTGCTCACCGGCGAGGTTCCGACTCCCGAGCAGGTTGCGGA
CGTGGAGGCCGAGTTCAAGACACGGCAGCAGGTTCCGGAATACGTGTTCCAGGCCTTGCGCGCGCTCCCGCTGGACAGCC
ACCCGATGGTGATGCTCTCCTCCGGCATCCTTGCCATGCAGAGGGATTCCAAGTTCGCAGCCTTCTACAGCAGCGGCAAG
TTTAACAAAATGACCGCCTGGGAACACGTTTACGAGGATGCGAGCGACATCGTGGCGCGCATCCCGGTACTGGCAGCCTT
CATCTACAATCTCAAGTACCGGGGCGACAAGCAGATCTCCATCGACCCGAAACTGGACCTGGGCGCCAACTTCGCCCAGA
TGATCGGGCAGAGCGAGCAGTACAAGGACGTGGCACGCATGTACTTCATCCTCCACTCCGACCACGAGTCGGGCAACGTC
TCGGCCCATGCCACCCACCTCGTCCACTCTGCCCTGTCCGATCCCTATTACGCATATGCCGCAGGTCTCGCCGGTTTGGC
AGGCCCCCTTCACGGCCTGGCGAACCAGGAGGTACTGGGGTGGATCCTGGAATTCCAGAAGAAGCTGAACGGCGCCGAGC
CTACCACGGAAAACGTCACCGCTGCTCTTTGGGATACCCTCAATGCCGGGCAGGTGGTCCCCGGTTACGGGCATGCCGTG
CTCAGGAAAACCGACCCGCGCTACATGGCTCAGCGCGAGTTCTGCCTGAAGACCACGGGGCTCAAGGACGACAAGCTCTT
CAAGCTGGTTTCGATGATTTTCGAGACCGCACCGGGCGTTCTTACCGAACATGGCAAGACCAAAAACCCGTGGCCCAACG
TCGATGCGCAATCGGGCGTGATCCAGTGGTATTACGGGCTGAAGGAATGGGATTTTTACACGGTGCTCTTTGGAGTGGGG
CGCGCACTGGGGTGCATGGCGAACATCACCTGGGATCGTGGCCTTGGCTACCCCATCGAGCGGCCCAAATCCGTCACCAC
CGACATGCTGGAGACCTGGGCTGCCGCCGGAGGCAGGGATATCGCCGCCCCCGCTTCACCCCAACCGCCAAAGCCAACGG
CTTAA

Upstream 100 bases:

>100_bases
GCTGGCTGGCGCGGGCTGACATGAGGGGTGCCAAAAGCGTGTTAAATCTGTCACGGGCGGACCTTCGCCCTAACCTTAAA
CGAAGAAAGGAGAACAGGAC

Downstream 100 bases:

>100_bases
GGGCAGTATGCATAAACGAGGGGGGGCAACCTGTGCCAGAAGATTTCCGTCCTGTCCAGGTCGCCCCCCTTCTTGTTTAG
CTGCCCATTCCGCTGATCCG

Product: type I citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTQLKDRLKEKIEAHRPRIARLTKEFGSVIIDKVDIAQCIGGARDIRSLVTDISYLDPQEGIRFRGKTIPETFEALPKAA
GSEYPTVESFWYFLLTGEVPTPEQVADVEAEFKTRQQVPEYVFQALRALPLDSHPMVMLSSGILAMQRDSKFAAFYSSGK
FNKMTAWEHVYEDASDIVARIPVLAAFIYNLKYRGDKQISIDPKLDLGANFAQMIGQSEQYKDVARMYFILHSDHESGNV
SAHATHLVHSALSDPYYAYAAGLAGLAGPLHGLANQEVLGWILEFQKKLNGAEPTTENVTAALWDTLNAGQVVPGYGHAV
LRKTDPRYMAQREFCLKTTGLKDDKLFKLVSMIFETAPGVLTEHGKTKNPWPNVDAQSGVIQWYYGLKEWDFYTVLFGVG
RALGCMANITWDRGLGYPIERPKSVTTDMLETWAAAGGRDIAAPASPQPPKPTA

Sequences:

>Translated_454_residues
MTQLKDRLKEKIEAHRPRIARLTKEFGSVIIDKVDIAQCIGGARDIRSLVTDISYLDPQEGIRFRGKTIPETFEALPKAA
GSEYPTVESFWYFLLTGEVPTPEQVADVEAEFKTRQQVPEYVFQALRALPLDSHPMVMLSSGILAMQRDSKFAAFYSSGK
FNKMTAWEHVYEDASDIVARIPVLAAFIYNLKYRGDKQISIDPKLDLGANFAQMIGQSEQYKDVARMYFILHSDHESGNV
SAHATHLVHSALSDPYYAYAAGLAGLAGPLHGLANQEVLGWILEFQKKLNGAEPTTENVTAALWDTLNAGQVVPGYGHAV
LRKTDPRYMAQREFCLKTTGLKDDKLFKLVSMIFETAPGVLTEHGKTKNPWPNVDAQSGVIQWYYGLKEWDFYTVLFGVG
RALGCMANITWDRGLGYPIERPKSVTTDMLETWAAAGGRDIAAPASPQPPKPTA
>Mature_453_residues
TQLKDRLKEKIEAHRPRIARLTKEFGSVIIDKVDIAQCIGGARDIRSLVTDISYLDPQEGIRFRGKTIPETFEALPKAAG
SEYPTVESFWYFLLTGEVPTPEQVADVEAEFKTRQQVPEYVFQALRALPLDSHPMVMLSSGILAMQRDSKFAAFYSSGKF
NKMTAWEHVYEDASDIVARIPVLAAFIYNLKYRGDKQISIDPKLDLGANFAQMIGQSEQYKDVARMYFILHSDHESGNVS
AHATHLVHSALSDPYYAYAAGLAGLAGPLHGLANQEVLGWILEFQKKLNGAEPTTENVTAALWDTLNAGQVVPGYGHAVL
RKTDPRYMAQREFCLKTTGLKDDKLFKLVSMIFETAPGVLTEHGKTKNPWPNVDAQSGVIQWYYGLKEWDFYTVLFGVGR
ALGCMANITWDRGLGYPIERPKSVTTDMLETWAAAGGRDIAAPASPQPPKPTA

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=431, Percent_Identity=48.7238979118329, Blast_Score=430, Evalue=1e-120,
Organism=Escherichia coli, GI1786939, Length=395, Percent_Identity=30.379746835443, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786527, Length=348, Percent_Identity=24.4252873563218, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17555174, Length=440, Percent_Identity=49.3181818181818, Blast_Score=439, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6324328, Length=426, Percent_Identity=53.0516431924883, Blast_Score=456, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6319850, Length=426, Percent_Identity=50.4694835680751, Blast_Score=431, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6325257, Length=454, Percent_Identity=39.8678414096916, Blast_Score=337, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24640126, Length=429, Percent_Identity=52.2144522144522, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI24640124, Length=429, Percent_Identity=52.2144522144522, Blast_Score=447, Evalue=1e-126,
Organism=Drosophila melanogaster, GI21356863, Length=427, Percent_Identity=41.92037470726, Blast_Score=347, Evalue=8e-96,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 50485; Mature: 50354

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLKDRLKEKIEAHRPRIARLTKEFGSVIIDKVDIAQCIGGARDIRSLVTDISYLDPQE
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHH
GIRFRGKTIPETFEALPKAAGSEYPTVESFWYFLLTGEVPTPEQVADVEAEFKTRQQVPE
CCEECCCCCHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
YVFQALRALPLDSHPMVMLSSGILAMQRDSKFAAFYSSGKFNKMTAWEHVYEDASDIVAR
HHHHHHHHCCCCCCCCEEEECCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
IPVLAAFIYNLKYRGDKQISIDPKLDLGANFAQMIGQSEQYKDVARMYFILHSDHESGNV
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHEECCCCCCCC
SAHATHLVHSALSDPYYAYAAGLAGLAGPLHGLANQEVLGWILEFQKKLNGAEPTTENVT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHH
AALWDTLNAGQVVPGYGHAVLRKTDPRYMAQREFCLKTTGLKDDKLFKLVSMIFETAPGV
HHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCH
LTEHGKTKNPWPNVDAQSGVIQWYYGLKEWDFYTVLFGVGRALGCMANITWDRGLGYPIE
HHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RPKSVTTDMLETWAAAGGRDIAAPASPQPPKPTA
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TQLKDRLKEKIEAHRPRIARLTKEFGSVIIDKVDIAQCIGGARDIRSLVTDISYLDPQE
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHH
GIRFRGKTIPETFEALPKAAGSEYPTVESFWYFLLTGEVPTPEQVADVEAEFKTRQQVPE
CCEECCCCCHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
YVFQALRALPLDSHPMVMLSSGILAMQRDSKFAAFYSSGKFNKMTAWEHVYEDASDIVAR
HHHHHHHHCCCCCCCCEEEECCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
IPVLAAFIYNLKYRGDKQISIDPKLDLGANFAQMIGQSEQYKDVARMYFILHSDHESGNV
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHCCCCHHHHHHHHHHHHEECCCCCCCC
SAHATHLVHSALSDPYYAYAAGLAGLAGPLHGLANQEVLGWILEFQKKLNGAEPTTENVT
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHH
AALWDTLNAGQVVPGYGHAVLRKTDPRYMAQREFCLKTTGLKDDKLFKLVSMIFETAPGV
HHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCH
LTEHGKTKNPWPNVDAQSGVIQWYYGLKEWDFYTVLFGVGRALGCMANITWDRGLGYPIE
HHCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
RPKSVTTDMLETWAAAGGRDIAAPASPQPPKPTA
CCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8532683; 9254593 [H]