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The map label for this gene is folD-1 [H]

Identifier: 197119292

GI number: 197119292

Start: 3368660

End: 3369532

Strand: Direct

Name: folD-1 [H]

Synonym: Gbem_2920

Alternate gene names: 197119292

Gene position: 3368660-3369532 (Clockwise)

Preceding gene: 197119286

Following gene: 197119297

Centisome position: 72.99

GC content: 61.74

Gene sequence:

>873_bases
ATGAATCTTTTAGACGGTAAGAAATGCGCCGACAGCCTGATCGCAGGCATAGCGAAACAGGTCGCGCGCCACGTCGACTT
CGGCCTCAGGAAACCGCACATGACGGTGGTCCTGGTCGGCAACCACGCACCCAGCGAATCGTACGTGAAATCCAAGGTCA
CCACCTGCACCCTTTCCGGCTTCGAGAGCAACCTGATCCGGCTTCCCGAGACCGTGACCGAGAAGGAGCTGCTCTGGCTG
ATCCAACAGATCAACGCCGACGCCTCCACCGACGGGGTCATCGTCCAGCTCCCGCTCCCCAAGCAGATCAACGCCCAGCG
GGTGATCAACGCGATAGCACCCGAGAAGGACATCGACGGCTTCCACCCCACCAACTTCGGGCGCATGGCGCTCGGGCAAA
AGGCCTTCCGCCCGGCGACCGCCTACGGCATCTGCAAGCTGCTGCAGTTCTACCAGGTGCCGGTCGCGGGTAAGCACTGC
GTGGTCATCGGACGCTCCAACATCGTCGGCAAGCCTATCTCCATAATGCTCTCCAACGACTTCGACATCGGCAACGCCAC
CGTCACGCTGACCCATATCGAGACGCCGCGTGAGCTGCTGCTCGACGAGACCCGCCGTGCCGACATCGTCATCGTTGCCG
TCGGCATCCCCGGCTTCGTCACGCCCGACATGGTCAAGGACGGGGTAACGCTTATAGACGTCGGCATCAACCGGCTGGAG
AACGGCAAGTTGGTTGGAGACGTGGATTTTGCTGCCGTCGCGCCCAAGTGCGAGTGGATCACGCCGGTCCCTGGCGGCGT
TGGGCGCATGACCGTCGCGGCGCTGATGATCAATACCCTGATGGCCTACCAGAACAACTTCGAGCTCGCCTAA

Upstream 100 bases:

>100_bases
CTAAGTAGCTGTTGAGGCGTTGCTTTTTTTGTGATACAAATTTTTGAGCAGGCCCAAGAAGTCATCTACAGGGGATGCAA
CCCATAGCTAAGGAACCAGC

Downstream 100 bases:

>100_bases
CAGCCTCGTACCCTCTCCCAGGGGGAGAGGGAGTTTTTTACGCAACGACAGGATTAAAAAAAGGGTCCCGGCATATTCCG
CCAGGACCCTTTTTCCATTT

Product: bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNLLDGKKCADSLIAGIAKQVARHVDFGLRKPHMTVVLVGNHAPSESYVKSKVTTCTLSGFESNLIRLPETVTEKELLWL
IQQINADASTDGVIVQLPLPKQINAQRVINAIAPEKDIDGFHPTNFGRMALGQKAFRPATAYGICKLLQFYQVPVAGKHC
VVIGRSNIVGKPISIMLSNDFDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTPDMVKDGVTLIDVGINRLE
NGKLVGDVDFAAVAPKCEWITPVPGGVGRMTVAALMINTLMAYQNNFELA

Sequences:

>Translated_290_residues
MNLLDGKKCADSLIAGIAKQVARHVDFGLRKPHMTVVLVGNHAPSESYVKSKVTTCTLSGFESNLIRLPETVTEKELLWL
IQQINADASTDGVIVQLPLPKQINAQRVINAIAPEKDIDGFHPTNFGRMALGQKAFRPATAYGICKLLQFYQVPVAGKHC
VVIGRSNIVGKPISIMLSNDFDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTPDMVKDGVTLIDVGINRLE
NGKLVGDVDFAAVAPKCEWITPVPGGVGRMTVAALMINTLMAYQNNFELA
>Mature_290_residues
MNLLDGKKCADSLIAGIAKQVARHVDFGLRKPHMTVVLVGNHAPSESYVKSKVTTCTLSGFESNLIRLPETVTEKELLWL
IQQINADASTDGVIVQLPLPKQINAQRVINAIAPEKDIDGFHPTNFGRMALGQKAFRPATAYGICKLLQFYQVPVAGKHC
VVIGRSNIVGKPISIMLSNDFDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTPDMVKDGVTLIDVGINRLE
NGKLVGDVDFAAVAPKCEWITPVPGGVGRMTVAALMINTLMAYQNNFELA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=44.9324324324324, Blast_Score=242, Evalue=3e-64,
Organism=Homo sapiens, GI222418558, Length=292, Percent_Identity=44.1780821917808, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI222136639, Length=300, Percent_Identity=39, Blast_Score=181, Evalue=9e-46,
Organism=Escherichia coli, GI1786741, Length=280, Percent_Identity=48.2142857142857, Blast_Score=244, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17568735, Length=274, Percent_Identity=40.5109489051095, Blast_Score=164, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6319558, Length=298, Percent_Identity=41.2751677852349, Blast_Score=206, Evalue=5e-54,
Organism=Saccharomyces cerevisiae, GI6321643, Length=306, Percent_Identity=40.8496732026144, Blast_Score=190, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6322933, Length=294, Percent_Identity=23.8095238095238, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17136818, Length=296, Percent_Identity=43.9189189189189, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI17136816, Length=296, Percent_Identity=43.9189189189189, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24645718, Length=284, Percent_Identity=38.7323943661972, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17137370, Length=284, Percent_Identity=38.7323943661972, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI62472483, Length=284, Percent_Identity=38.7323943661972, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI45551871, Length=284, Percent_Identity=38.7323943661972, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31392; Mature: 31392

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00766 THF_DHG_CYH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLDGKKCADSLIAGIAKQVARHVDFGLRKPHMTVVLVGNHAPSESYVKSKVTTCTLSG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHEEEEECC
FESNLIRLPETVTEKELLWLIQQINADASTDGVIVQLPLPKQINAQRVINAIAPEKDIDG
CCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCC
FHPTNFGRMALGQKAFRPATAYGICKLLQFYQVPVAGKHCVVIGRSNIVGKPISIMLSND
CCCCCCCHHHHCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECC
FDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTPDMVKDGVTLIDVGINRLE
CCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHCCCEEEEECCCCCC
NGKLVGDVDFAAVAPKCEWITPVPGGVGRMTVAALMINTLMAYQNNFELA
CCCEEECCCHHEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNLLDGKKCADSLIAGIAKQVARHVDFGLRKPHMTVVLVGNHAPSESYVKSKVTTCTLSG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHEEEEECC
FESNLIRLPETVTEKELLWLIQQINADASTDGVIVQLPLPKQINAQRVINAIAPEKDIDG
CCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCC
FHPTNFGRMALGQKAFRPATAYGICKLLQFYQVPVAGKHCVVIGRSNIVGKPISIMLSND
CCCCCCCHHHHCHHHCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEEECC
FDIGNATVTLTHIETPRELLLDETRRADIVIVAVGIPGFVTPDMVKDGVTLIDVGINRLE
CCCCCEEEEEEECCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHCCCEEEEECCCCCC
NGKLVGDVDFAAVAPKCEWITPVPGGVGRMTVAALMINTLMAYQNNFELA
CCCEEECCCHHEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA