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The map label for this gene is aspS

Identifier: 197119275

GI number: 197119275

Start: 3348750

End: 3350531

Strand: Reverse

Name: aspS

Synonym: Gbem_2903

Alternate gene names: 197119275

Gene position: 3350531-3348750 (Counterclockwise)

Preceding gene: 197119276

Following gene: 197119274

Centisome position: 72.6

GC content: 62.74

Gene sequence:

>1782_bases
ATGATAGATTTTCTGGGTGATTGGAAGAGAAGCAGTTACTGCGGCGAGTTGAGGGGGGGCCACATAGGGCAGGAAGTCAC
CCTCATGGGTTGGGTCTCCAAGAGGCGCGACCACGGCGGGCTCATCTTCGTCGACCTGCGCGACAGGGACGGCATCTCCC
AGATCGTCTTCGACCCGGAGCGCTGTCCCGAGGCCCACGCCAAGGCGGAGTCCGCCAGGAACGAGTACGTCCTTGCCATC
AGGGGTACGGTTGCCGCCCGCCCGGAAGGGACCGTCAACCCGAAGATGAAGACCGGCGAGATCGAGGTGCTGGTGACCGA
GTGCAAGCTTTTGAACCCCTCCAAGGCGCTCCCTTTCACGCTGGACGGCTTCGTGGACGTCAACGAGAACATCAGGCTCA
AGTACCGCTATCTCGACCTTCGCACCGGGACGCTGCAGCAGAACCTGATGCTCCGCTCCAAGGTGGCCCAGTTGACCCGC
TCCTACTTGAGCGACAACGGCTTCATCGAACTGGAGACCCCTTTCCTCACCAAGTCCACCCCGGAAGGGGCGCGCGACTT
CCTGGTTCCCTCCCGCATCAACAAGGGCGAGTTCTACGCGCTGCCGCAGTCCCCGCAGCTCTTCAAGCAGATCCTCATGG
TCTCCGGCTTTGACCGCTACTTCCAGATCGTGCGCTGCTTCCGCGACGAGGACCTGCGCGCCGACCGCCAGCCCGAGTTC
ACCCAGATCGACTGCGAGATGTCCTTCATCGACCGCGAGGACATCATCACCGTGATGGAAGGGCTCATGGCCCGCATCTT
CACCGAGGCCAGAGGGGTCAAGGTTGAGCTCCCGATCGAGAGGATGACCTACCAGGAGGCGATCCGCCGCTTCGGCGTGG
ACAACCCGGACCTTCGTTTTGGGCTCGAACTGGTAGAGCTCTCCGACATCGTCAAGAACTCCGGTTTCAAGGTCTTCGCC
GACGTGGTGAACGGCGGCGGGATCGTCAAGGGTATGAACGTCAAGGGCGCCGGCGCCATGAGCCGCAAGGAAATCGACGA
CCTGACCGAGTTCGCCAAGATCTACGGCGCCAAGGGTCTCGCCTACGTGAAGATGACCGCCGAAGGGTGGCAATCCCCCA
TCGCCAAGTTCTTCACCCCCGAAGAGATCGCCACCATGGATAAGGCCTTCGACGCCAAAGAAGGGGACCTGCTCCTCTTC
GTCGCCGACAAGCCGAAGGTGGTCAACGATTCGCTGGGCAAACTCAGGAATCATCTCGCCGCCAAGATGGGGCTCACCGA
TCCCAACGTCTTCCGCTTCGTCTGGATCACCGACTTCCCGCTTCTCGAGTGGGACGAGGAAGCGAAACGCTGGGCGGCGG
TGCACCACCCCTTCACCGCGCCCATGGACGAGGACCTGCAGTACGTCGAGAGCGATCCCGGCCGCTGCCGCGCCAAGGCC
TACGACCTGGTCCTTAACGGCAACGAGATCGGCGGCGGCTCCATCAGGATCCATCAGGAGGAAGTGCAGTCCCTCATGTT
CAAGATGCTGGGGCTCTCCGAGGAGAACGCCCGCTCCAAGTTCGGCTTCCTGCTCGACGCGCTCGAGTACGGCACCCCGC
CGCACGGCGGCATCGCCTTCGGCCTGGACCGCCTGATGATGCTGATCACCGGTAGCGACTCGATCCGCGACGTCATCGCT
TTCCCGAAGACCCAGAAGGGGGCCTGCCTCATGTCGGACGCCCCCTCCGGTGTCGATGCACTGCAGCTGCGCGAGCTCGG
CATTCGTCTCGCCGCCAAGTAG

Upstream 100 bases:

>100_bases
TCCCGCGGCGCCCAAATCCCCCGTTTTTGGTTGAAGCTCACTTTCTTTTTGGGTATAGTGACCCGTTATTTCTTGCAGAT
TAATTCCAAGGAGGAGTTCG

Downstream 100 bases:

>100_bases
GGGGGCTGTTGAAGCTCGCCGTCCGGTTCATAACTCTTTTGCTCGTCATTTCAGCGGCGGCCTGCGCAACGCAGCCGCCG
CGTTTTCGCGATGAGGCGAG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MIDFLGDWKRSSYCGELRGGHIGQEVTLMGWVSKRRDHGGLIFVDLRDRDGISQIVFDPERCPEAHAKAESARNEYVLAI
RGTVAARPEGTVNPKMKTGEIEVLVTECKLLNPSKALPFTLDGFVDVNENIRLKYRYLDLRTGTLQQNLMLRSKVAQLTR
SYLSDNGFIELETPFLTKSTPEGARDFLVPSRINKGEFYALPQSPQLFKQILMVSGFDRYFQIVRCFRDEDLRADRQPEF
TQIDCEMSFIDREDIITVMEGLMARIFTEARGVKVELPIERMTYQEAIRRFGVDNPDLRFGLELVELSDIVKNSGFKVFA
DVVNGGGIVKGMNVKGAGAMSRKEIDDLTEFAKIYGAKGLAYVKMTAEGWQSPIAKFFTPEEIATMDKAFDAKEGDLLLF
VADKPKVVNDSLGKLRNHLAAKMGLTDPNVFRFVWITDFPLLEWDEEAKRWAAVHHPFTAPMDEDLQYVESDPGRCRAKA
YDLVLNGNEIGGGSIRIHQEEVQSLMFKMLGLSEENARSKFGFLLDALEYGTPPHGGIAFGLDRLMMLITGSDSIRDVIA
FPKTQKGACLMSDAPSGVDALQLRELGIRLAAK

Sequences:

>Translated_593_residues
MIDFLGDWKRSSYCGELRGGHIGQEVTLMGWVSKRRDHGGLIFVDLRDRDGISQIVFDPERCPEAHAKAESARNEYVLAI
RGTVAARPEGTVNPKMKTGEIEVLVTECKLLNPSKALPFTLDGFVDVNENIRLKYRYLDLRTGTLQQNLMLRSKVAQLTR
SYLSDNGFIELETPFLTKSTPEGARDFLVPSRINKGEFYALPQSPQLFKQILMVSGFDRYFQIVRCFRDEDLRADRQPEF
TQIDCEMSFIDREDIITVMEGLMARIFTEARGVKVELPIERMTYQEAIRRFGVDNPDLRFGLELVELSDIVKNSGFKVFA
DVVNGGGIVKGMNVKGAGAMSRKEIDDLTEFAKIYGAKGLAYVKMTAEGWQSPIAKFFTPEEIATMDKAFDAKEGDLLLF
VADKPKVVNDSLGKLRNHLAAKMGLTDPNVFRFVWITDFPLLEWDEEAKRWAAVHHPFTAPMDEDLQYVESDPGRCRAKA
YDLVLNGNEIGGGSIRIHQEEVQSLMFKMLGLSEENARSKFGFLLDALEYGTPPHGGIAFGLDRLMMLITGSDSIRDVIA
FPKTQKGACLMSDAPSGVDALQLRELGIRLAAK
>Mature_593_residues
MIDFLGDWKRSSYCGELRGGHIGQEVTLMGWVSKRRDHGGLIFVDLRDRDGISQIVFDPERCPEAHAKAESARNEYVLAI
RGTVAARPEGTVNPKMKTGEIEVLVTECKLLNPSKALPFTLDGFVDVNENIRLKYRYLDLRTGTLQQNLMLRSKVAQLTR
SYLSDNGFIELETPFLTKSTPEGARDFLVPSRINKGEFYALPQSPQLFKQILMVSGFDRYFQIVRCFRDEDLRADRQPEF
TQIDCEMSFIDREDIITVMEGLMARIFTEARGVKVELPIERMTYQEAIRRFGVDNPDLRFGLELVELSDIVKNSGFKVFA
DVVNGGGIVKGMNVKGAGAMSRKEIDDLTEFAKIYGAKGLAYVKMTAEGWQSPIAKFFTPEEIATMDKAFDAKEGDLLLF
VADKPKVVNDSLGKLRNHLAAKMGLTDPNVFRFVWITDFPLLEWDEEAKRWAAVHHPFTAPMDEDLQYVESDPGRCRAKA
YDLVLNGNEIGGGSIRIHQEEVQSLMFKMLGLSEENARSKFGFLLDALEYGTPPHGGIAFGLDRLMMLITGSDSIRDVIA
FPKTQKGACLMSDAPSGVDALQLRELGIRLAAK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=593, Percent_Identity=44.3507588532884, Blast_Score=489, Evalue=1e-138,
Organism=Homo sapiens, GI194272210, Length=249, Percent_Identity=29.3172690763052, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI5031815, Length=266, Percent_Identity=29.3233082706767, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI45439306, Length=175, Percent_Identity=32, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI4758762, Length=285, Percent_Identity=27.3684210526316, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=51.7766497461929, Blast_Score=613, Evalue=1e-176,
Organism=Escherichia coli, GI1790571, Length=283, Percent_Identity=30.3886925795053, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1789256, Length=317, Percent_Identity=28.391167192429, Blast_Score=105, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI32566633, Length=604, Percent_Identity=36.0927152317881, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI71994340, Length=259, Percent_Identity=27.027027027027, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17535927, Length=259, Percent_Identity=27.027027027027, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17535925, Length=259, Percent_Identity=27.027027027027, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=172, Percent_Identity=29.0697674418605, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6325153, Length=643, Percent_Identity=32.6594090202177, Blast_Score=296, Evalue=8e-81,
Organism=Saccharomyces cerevisiae, GI6323011, Length=304, Percent_Identity=28.2894736842105, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320242, Length=260, Percent_Identity=26.5384615384615, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321807, Length=249, Percent_Identity=26.9076305220884, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=123, Percent_Identity=29.2682926829268, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24584738, Length=589, Percent_Identity=39.3887945670628, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24640849, Length=280, Percent_Identity=30, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24640851, Length=280, Percent_Identity=30, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17136276, Length=176, Percent_Identity=31.25, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19921528, Length=288, Percent_Identity=26.3888888888889, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_GEOBB (B5EIV1)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139702.1
- GeneID:   6779756
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2903
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66560; Mature: 66560

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFLGDWKRSSYCGELRGGHIGQEVTLMGWVSKRRDHGGLIFVDLRDRDGISQIVFDPE
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCEEEEEECCCCCCHHEEECHH
RCPEAHAKAESARNEYVLAIRGTVAARPEGTVNPKMKTGEIEVLVTECKLLNPSKALPFT
HCCHHHHHHHHCCCCEEEEEECEEEECCCCCCCCCEECCCEEEEEEEEEECCCCCCCCEE
LDGFVDVNENIRLKYRYLDLRTGTLQQNLMLRSKVAQLTRSYLSDNGFIELETPFLTKST
ECCEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PEGARDFLVPSRINKGEFYALPQSPQLFKQILMVSGFDRYFQIVRCFRDEDLRADRQPEF
CCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
TQIDCEMSFIDREDIITVMEGLMARIFTEARGVKVELPIERMTYQEAIRRFGVDNPDLRF
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCHHH
GLELVELSDIVKNSGFKVFADVVNGGGIVKGMNVKGAGAMSRKEIDDLTEFAKIYGAKGL
HHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCE
AYVKMTAEGWQSPIAKFFTPEEIATMDKAFDAKEGDLLLFVADKPKVVNDSLGKLRNHLA
EEEEEEHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
AKMGLTDPNVFRFVWITDFPLLEWDEEAKRWAAVHHPFTAPMDEDLQYVESDPGRCRAKA
HHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEE
YDLVLNGNEIGGGSIRIHQEEVQSLMFKMLGLSEENARSKFGFLLDALEYGTPPHGGIAF
EEEEEECCEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
GLDRLMMLITGSDSIRDVIAFPKTQKGACLMSDAPSGVDALQLRELGIRLAAK
HHHHHEEEEECCCHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCEEECCC
>Mature Secondary Structure
MIDFLGDWKRSSYCGELRGGHIGQEVTLMGWVSKRRDHGGLIFVDLRDRDGISQIVFDPE
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHCCCCCCCEEEEEECCCCCCHHEEECHH
RCPEAHAKAESARNEYVLAIRGTVAARPEGTVNPKMKTGEIEVLVTECKLLNPSKALPFT
HCCHHHHHHHHCCCCEEEEEECEEEECCCCCCCCCEECCCEEEEEEEEEECCCCCCCCEE
LDGFVDVNENIRLKYRYLDLRTGTLQQNLMLRSKVAQLTRSYLSDNGFIELETPFLTKST
ECCEEECCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
PEGARDFLVPSRINKGEFYALPQSPQLFKQILMVSGFDRYFQIVRCFRDEDLRADRQPEF
CCCHHHCCCCCCCCCCCEEECCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCC
TQIDCEMSFIDREDIITVMEGLMARIFTEARGVKVELPIERMTYQEAIRRFGVDNPDLRF
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCHHH
GLELVELSDIVKNSGFKVFADVVNGGGIVKGMNVKGAGAMSRKEIDDLTEFAKIYGAKGL
HHHHHHHHHHHHCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCE
AYVKMTAEGWQSPIAKFFTPEEIATMDKAFDAKEGDLLLFVADKPKVVNDSLGKLRNHLA
EEEEEEHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHH
AKMGLTDPNVFRFVWITDFPLLEWDEEAKRWAAVHHPFTAPMDEDLQYVESDPGRCRAKA
HHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEE
YDLVLNGNEIGGGSIRIHQEEVQSLMFKMLGLSEENARSKFGFLLDALEYGTPPHGGIAF
EEEEEECCEECCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHH
GLDRLMMLITGSDSIRDVIAFPKTQKGACLMSDAPSGVDALQLRELGIRLAAK
HHHHHEEEEECCCHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA