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The map label for this gene is pyrF

Identifier: 197119262

GI number: 197119262

Start: 3332618

End: 3333340

Strand: Reverse

Name: pyrF

Synonym: Gbem_2890

Alternate gene names: 197119262

Gene position: 3333340-3332618 (Counterclockwise)

Preceding gene: 197119263

Following gene: 197119261

Centisome position: 72.23

GC content: 62.52

Gene sequence:

>723_bases
ATGACCAGAGAAGAAGCAATCAAGAAGATAATCTTCGCCATGGATGTGAAGGAGTTCAGCGACGTACAGTACTGGGCCGA
ACTCCTCTCGCAGCACGTCGGCATGTTCAAGGTCGGCAAGCAGCTCTACACCGCCTGCGGTCCCGCCGCGGTCCGCATGA
TCCAGAAGTGCGGCGGTGAAGTGTTCCTCGACCTCAAGTACCACGACATCCCGAACACCGTCGCCATGGCGACCCTCGAA
GCCGCGAACCTCGGCGTGCAGCTCTGCGACCTGCATGCCATGGGCGGCTACGAGATGATGAACAAGACCATGGAGACCCT
GGACAAGAACTTCAGCGGCTGCACCGCGCGTCCCAAGGTCCTCGCCATCACCGTCCTCACCTCCTCCAACGAAGAGACCC
TGCGCGGCATCGGGATCGAGCTCCCCGTCCCCGAGATGGTGGTGAAACTTGCCAAGCTGGCGAAAAGCGCGGGCGTCGAC
GGCGTCGTCGCCTCCCCGCAGGAAGTCGAGCTGATCAGGGAAGCGTGCGGCAAAGATTTCCTCGTCGTTACCCCCGGAGT
CCGTCCGAGCTTCGCGTCCGCTGACGACCAGAAGCGCATCATGTCCCCCGCCGAAGCCGTCAAGGCCGGAGCCGACTATC
TCGTCATAGGCCGCCCCATCGCCGCCGCCCAAAGCCCGGTCGAAGCCGCCCAGAAGATCGTCGACGAGATAGTAGCCGGG
TAA

Upstream 100 bases:

>100_bases
GCCAAAGGTTTTGACTAAAGCTTTGAGATCCACCAAAAGCTTTTCTCAGAAGTTCTCAGATTTTCTCCTTGCCAACGGTT
TTGACTTAAGGATTTTGATT

Downstream 100 bases:

>100_bases
TACTTCATTTTTTTTGAGAAACACCTTTTCATGTCCCCTCCCCCAATGGTAGAGGGGCAAGTCCATCCCCTCGCCCTCTG
GGAGAGCGGCAGGGGTGAGG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTREEAIKKIIFAMDVKEFSDVQYWAELLSQHVGMFKVGKQLYTACGPAAVRMIQKCGGEVFLDLKYHDIPNTVAMATLE
AANLGVQLCDLHAMGGYEMMNKTMETLDKNFSGCTARPKVLAITVLTSSNEETLRGIGIELPVPEMVVKLAKLAKSAGVD
GVVASPQEVELIREACGKDFLVVTPGVRPSFASADDQKRIMSPAEAVKAGADYLVIGRPIAAAQSPVEAAQKIVDEIVAG

Sequences:

>Translated_240_residues
MTREEAIKKIIFAMDVKEFSDVQYWAELLSQHVGMFKVGKQLYTACGPAAVRMIQKCGGEVFLDLKYHDIPNTVAMATLE
AANLGVQLCDLHAMGGYEMMNKTMETLDKNFSGCTARPKVLAITVLTSSNEETLRGIGIELPVPEMVVKLAKLAKSAGVD
GVVASPQEVELIREACGKDFLVVTPGVRPSFASADDQKRIMSPAEAVKAGADYLVIGRPIAAAQSPVEAAQKIVDEIVAG
>Mature_239_residues
TREEAIKKIIFAMDVKEFSDVQYWAELLSQHVGMFKVGKQLYTACGPAAVRMIQKCGGEVFLDLKYHDIPNTVAMATLEA
ANLGVQLCDLHAMGGYEMMNKTMETLDKNFSGCTARPKVLAITVLTSSNEETLRGIGIELPVPEMVVKLAKLAKSAGVDG
VVASPQEVELIREACGKDFLVVTPGVRPSFASADDQKRIMSPAEAVKAGADYLVIGRPIAAAQSPVEAAQKIVDEIVAG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=224, Percent_Identity=39.2857142857143, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_GEOBB (B5EIT8)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139689.1
- ProteinModelPortal:   B5EIT8
- GeneID:   6779743
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2890
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   CLSK828364
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25788; Mature: 25657

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 66-66 BINDING 15-15 BINDING 37-37 BINDING 127-127 BINDING 188-188 BINDING 197-197 BINDING 217-217 BINDING 218-218

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTREEAIKKIIFAMDVKEFSDVQYWAELLSQHVGMFKVGKQLYTACGPAAVRMIQKCGGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
VFLDLKYHDIPNTVAMATLEAANLGVQLCDLHAMGGYEMMNKTMETLDKNFSGCTARPKV
EEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCE
LAITVLTSSNEETLRGIGIELPVPEMVVKLAKLAKSAGVDGVVASPQEVELIREACGKDF
EEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCE
LVVTPGVRPSFASADDQKRIMSPAEAVKAGADYLVIGRPIAAAQSPVEAAQKIVDEIVAG
EEECCCCCCCCCCCCHHHHHCCHHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TREEAIKKIIFAMDVKEFSDVQYWAELLSQHVGMFKVGKQLYTACGPAAVRMIQKCGGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
VFLDLKYHDIPNTVAMATLEAANLGVQLCDLHAMGGYEMMNKTMETLDKNFSGCTARPKV
EEEEEEECCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCE
LAITVLTSSNEETLRGIGIELPVPEMVVKLAKLAKSAGVDGVVASPQEVELIREACGKDF
EEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCE
LVVTPGVRPSFASADDQKRIMSPAEAVKAGADYLVIGRPIAAAQSPVEAAQKIVDEIVAG
EEECCCCCCCCCCCCHHHHHCCHHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA