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The map label for this gene is rlmB [H]

Identifier: 197119261

GI number: 197119261

Start: 3331714

End: 3332460

Strand: Reverse

Name: rlmB [H]

Synonym: Gbem_2889

Alternate gene names: 197119261

Gene position: 3332460-3331714 (Counterclockwise)

Preceding gene: 197119262

Following gene: 197119260

Centisome position: 72.21

GC content: 61.04

Gene sequence:

>747_bases
ATGTCAAAAGAAGAAATCATCTACGGCCTGAATCCGGTAATGGAAGCACTGCGCGGCTCGCGCGAGATCCACGAGCTTTT
CGTAGCCGGCACCAGCGCAGACAAGCGCCTGGAGAAGCTCCTCAAGCTCGCCGCCGAGCGTAAGATCCCTGTGCGTCAGC
GTGAGAAGGGCGACCTCAACCGTCTGTGCGGCACCGAGCACCATCAAGGCGTGGCCCTCAAGGTGGAGCCCTTTCCCTAC
GCCGATCTGGACGACGTACTGGAGGGACTGCAGGGTACCCGCGACGGTCTCATTCTGGTTCTCGACAGCGTGCAGGACCC
GCACAACCTCGGTGCCCTCATCCGCAGCGCTGCCTGCGCGGGAGCAAACGCCGTCGTCATCCCCAAAGACCGTGCAGCAG
GAGTAACGGCAGTAGCCGAGAAGTCTTCAGCCGGCGCCACCGAGACCATCTCCGTCGCGCAGGTAACCAACATCTCCCAG
GCGCTGGAGAGTCTGAAGAAAGCAGGCTTCTGGATCTACGGAGCCGACGGCTCCGCGAGGCACACCCTCTACCAGCAGGA
CTTCACTGGACCGGTAGCACTCGTAATAGGTGGGGAAGGGGAGGGTATCCGTCCGCTGGTCCGAAAAGGATGCGATGCGG
TCGTGAGCATTCCGCTGCAGGGAGGCGTGAACTCGTTAAATGCCTCCGTAGCCGGAGGAATCTTCCTGTTCGAGGTCGTG
CGGCAGAGGGTTTCTGTGAAGAAGTAA

Upstream 100 bases:

>100_bases
CAAGTCCATCCCCTCGCCCTCTGGGAGAGCGGCAGGGGTGAGGGAGTTTTTCTTCAGAAATTCTCCCTGGCCAATGGTTT
AAAAGGTTTTTGAGAGGAAT

Downstream 100 bases:

>100_bases
TTAAAATGCTTGTGGAGTGATGTTTACATTGACTCGCTCTGAGCGCTGTAGTATAAGAGTTGAGTTGACCATGACGCGGG
GTGGAGCAGTCTGGTAGCTC

Product: 23S rRNA (2'-O-methyl-G2251)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSKEEIIYGLNPVMEALRGSREIHELFVAGTSADKRLEKLLKLAAERKIPVRQREKGDLNRLCGTEHHQGVALKVEPFPY
ADLDDVLEGLQGTRDGLILVLDSVQDPHNLGALIRSAACAGANAVVIPKDRAAGVTAVAEKSSAGATETISVAQVTNISQ
ALESLKKAGFWIYGADGSARHTLYQQDFTGPVALVIGGEGEGIRPLVRKGCDAVVSIPLQGGVNSLNASVAGGIFLFEVV
RQRVSVKK

Sequences:

>Translated_248_residues
MSKEEIIYGLNPVMEALRGSREIHELFVAGTSADKRLEKLLKLAAERKIPVRQREKGDLNRLCGTEHHQGVALKVEPFPY
ADLDDVLEGLQGTRDGLILVLDSVQDPHNLGALIRSAACAGANAVVIPKDRAAGVTAVAEKSSAGATETISVAQVTNISQ
ALESLKKAGFWIYGADGSARHTLYQQDFTGPVALVIGGEGEGIRPLVRKGCDAVVSIPLQGGVNSLNASVAGGIFLFEVV
RQRVSVKK
>Mature_247_residues
SKEEIIYGLNPVMEALRGSREIHELFVAGTSADKRLEKLLKLAAERKIPVRQREKGDLNRLCGTEHHQGVALKVEPFPYA
DLDDVLEGLQGTRDGLILVLDSVQDPHNLGALIRSAACAGANAVVIPKDRAAGVTAVAEKSSAGATETISVAQVTNISQA
LESLKKAGFWIYGADGSARHTLYQQDFTGPVALVIGGEGEGIRPLVRKGCDAVVSIPLQGGVNSLNASVAGGIFLFEVVR
QRVSVKK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=264, Percent_Identity=31.8181818181818, Blast_Score=117, Evalue=8e-27,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=41.9753086419753, Blast_Score=190, Evalue=7e-50,
Organism=Escherichia coli, GI1788935, Length=244, Percent_Identity=27.8688524590164, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790083, Length=172, Percent_Identity=30.8139534883721, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=257, Percent_Identity=29.5719844357977, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324775, Length=286, Percent_Identity=27.972027972028, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26360; Mature: 26228

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEEIIYGLNPVMEALRGSREIHELFVAGTSADKRLEKLLKLAAERKIPVRQREKGDLN
CCCCCEEECCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
RLCGTEHHQGVALKVEPFPYADLDDVLEGLQGTRDGLILVLDSVQDPHNLGALIRSAACA
HHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHC
GANAVVIPKDRAAGVTAVAEKSSAGATETISVAQVTNISQALESLKKAGFWIYGADGSAR
CCCEEEEECCCCCCCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCEEEECCCCCCH
HTLYQQDFTGPVALVIGGEGEGIRPLVRKGCDAVVSIPLQGGVNSLNASVAGGIFLFEVV
HHEECCCCCCCEEEEECCCCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
RQRVSVKK
HHHHCCCC
>Mature Secondary Structure 
SKEEIIYGLNPVMEALRGSREIHELFVAGTSADKRLEKLLKLAAERKIPVRQREKGDLN
CCCCEEECCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHH
RLCGTEHHQGVALKVEPFPYADLDDVLEGLQGTRDGLILVLDSVQDPHNLGALIRSAACA
HHCCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHC
GANAVVIPKDRAAGVTAVAEKSSAGATETISVAQVTNISQALESLKKAGFWIYGADGSAR
CCCEEEEECCCCCCCCHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCEEEECCCCCCH
HTLYQQDFTGPVALVIGGEGEGIRPLVRKGCDAVVSIPLQGGVNSLNASVAGGIFLFEVV
HHEECCCCCCCEEEEECCCCCCCHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
RQRVSVKK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]