Click here to switch to the map view.

The map label for this gene is nifR3 [H]

Identifier: 197119180

GI number: 197119180

Start: 3236904

End: 3237869

Strand: Reverse

Name: nifR3 [H]

Synonym: Gbem_2807

Alternate gene names: 197119180

Gene position: 3237869-3236904 (Counterclockwise)

Preceding gene: 197119181

Following gene: 197119179

Centisome position: 70.16

GC content: 65.01

Gene sequence:

>966_bases
ATGCAAAAAACGGTGGCCCTCGGCCCACTTGTGTTGAAGAATCAACTATTTCTGGCCCCGATGGCGGGGATTACCAATCT
GCCGATGCGGATCATCTCCCGCGAGGGAGGGGCCTCGTTCGCCTTCACCGAGATGGTAAGCGTGAACGGCCTGACCCGGG
AAGGGCAAAAAAGTTTCGATCTTTTAAAGAGTAGCCCGGAGGACCGCCCCATCGGGATGCAGCTTTTCGGCGACGATCCG
GAGATGCTGGCCGAGGCGGCCCGCCTGGTGGAAGAGTACGGCGAGCTGATCGACATCAACATGGGATGTCCGGTGCGCAA
GGTGGTGGGAACCGGCGCCGGGAGCGCGCTGATGAAGGACCCGCAAAAGGTGGGGCGCATCGTCAGGAGCGTGAGGGCCG
CAACGAAGCTTCCGCTCACCATCAAGATACGCACCGGCTGGGTCTGCGGCGACGACACCTTCCTGGAAGTGGGGAGAATC
GCCCAGGAGGAGGGGTGCGACGCGGTAACGCTGCATCCCAGAAGCCGCGCCCAGATGTTCGAGGGGAAGGCCGACTGGTC
CCGGATCGGCGAGCTGAAGAGCGCGCTCAGCATCCCGGTGATCGGCAGCGGCGATCTCTTCAGCGCGGCCGACGCGGTGG
GGATGCTGGCCGAAACCGGGTGCGACTCCGTCATGGTTGCCCGCGGCGCCATGGGGAACCCCTGGATCTTCCGCGAGGCG
CTTTCCCTTTTGGCCGGTGAGGAGCCGGCTCCCCCGACGGTGCAAGAGCGGCTGGCGGTCTCCCGCAGGCACCTGGAGCT
TTTCGCCGAGTTCGCGGGGGGGCGGGTGGCGCTCATGGAGATGCGCAAGCACCTCTCCTGGTATTCCAAGGGGCTGCCTG
GGGCGGCGCAGTTTCGCGCGGCGGTGAACCGGATCGAAAGCGCTCCGGAGCTGATCCGGGCGATGGAGGAGTTCTTCGAT
GCCTGA

Upstream 100 bases:

>100_bases
CCCTTCCTTCGTTGAACCCCGCCGATAAAAAAACCTTGCCATACCAATTCCTTACTGCTAGTATTGCTCATTTTTTAGGC
AGATAAGGAACGATCGCTTT

Downstream 100 bases:

>100_bases
GAAACTGGAGAGTTACTACGCCAACGTGATCGACAGCGTGGGTGACGGCGTGATCGTCCTCGACAACGCCGGGGCCGTGA
CGCTGGTCAATCCCGCCGCC

Product: oxidoreductase NifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MQKTVALGPLVLKNQLFLAPMAGITNLPMRIISREGGASFAFTEMVSVNGLTREGQKSFDLLKSSPEDRPIGMQLFGDDP
EMLAEAARLVEEYGELIDINMGCPVRKVVGTGAGSALMKDPQKVGRIVRSVRAATKLPLTIKIRTGWVCGDDTFLEVGRI
AQEEGCDAVTLHPRSRAQMFEGKADWSRIGELKSALSIPVIGSGDLFSAADAVGMLAETGCDSVMVARGAMGNPWIFREA
LSLLAGEEPAPPTVQERLAVSRRHLELFAEFAGGRVALMEMRKHLSWYSKGLPGAAQFRAAVNRIESAPELIRAMEEFFD
A

Sequences:

>Translated_321_residues
MQKTVALGPLVLKNQLFLAPMAGITNLPMRIISREGGASFAFTEMVSVNGLTREGQKSFDLLKSSPEDRPIGMQLFGDDP
EMLAEAARLVEEYGELIDINMGCPVRKVVGTGAGSALMKDPQKVGRIVRSVRAATKLPLTIKIRTGWVCGDDTFLEVGRI
AQEEGCDAVTLHPRSRAQMFEGKADWSRIGELKSALSIPVIGSGDLFSAADAVGMLAETGCDSVMVARGAMGNPWIFREA
LSLLAGEEPAPPTVQERLAVSRRHLELFAEFAGGRVALMEMRKHLSWYSKGLPGAAQFRAAVNRIESAPELIRAMEEFFD
A
>Mature_321_residues
MQKTVALGPLVLKNQLFLAPMAGITNLPMRIISREGGASFAFTEMVSVNGLTREGQKSFDLLKSSPEDRPIGMQLFGDDP
EMLAEAARLVEEYGELIDINMGCPVRKVVGTGAGSALMKDPQKVGRIVRSVRAATKLPLTIKIRTGWVCGDDTFLEVGRI
AQEEGCDAVTLHPRSRAQMFEGKADWSRIGELKSALSIPVIGSGDLFSAADAVGMLAETGCDSVMVARGAMGNPWIFREA
LSLLAGEEPAPPTVQERLAVSRRHLELFAEFAGGRVALMEMRKHLSWYSKGLPGAAQFRAAVNRIESAPELIRAMEEFFD
A

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=241, Percent_Identity=35.6846473029046, Blast_Score=134, Evalue=8e-32,
Organism=Homo sapiens, GI239788462, Length=233, Percent_Identity=35.6223175965665, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI40807366, Length=225, Percent_Identity=30.2222222222222, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI8923374, Length=310, Percent_Identity=30.3225806451613, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI31742496, Length=238, Percent_Identity=31.0924369747899, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1789660, Length=320, Percent_Identity=39.375, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788462, Length=317, Percent_Identity=28.7066246056782, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI145693211, Length=325, Percent_Identity=26.7692307692308, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543114, Length=240, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17507177, Length=249, Percent_Identity=29.3172690763052, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI25144369, Length=248, Percent_Identity=28.2258064516129, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=253, Percent_Identity=29.2490118577075, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323560, Length=276, Percent_Identity=27.1739130434783, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323433, Length=295, Percent_Identity=29.8305084745763, Blast_Score=109, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6323437, Length=223, Percent_Identity=27.8026905829596, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24585320, Length=318, Percent_Identity=32.7044025157233, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24580595, Length=224, Percent_Identity=30.8035714285714, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19920448, Length=224, Percent_Identity=30.8035714285714, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19921524, Length=310, Percent_Identity=25.8064516129032, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45549423, Length=248, Percent_Identity=29.8387096774194, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 34780; Mature: 34780

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTVALGPLVLKNQLFLAPMAGITNLPMRIISREGGASFAFTEMVSVNGLTREGQKSFD
CCCCCCCCHHHHHCCHHHHHHCCHHHCCHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHH
LLKSSPEDRPIGMQLFGDDPEMLAEAARLVEEYGELIDINMGCPVRKVVGTGAGSALMKD
HHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCEEECCCCCCHHHHHCCCCCHHHHCC
PQKVGRIVRSVRAATKLPLTIKIRTGWVCGDDTFLEVGRIAQEEGCDAVTLHPRSRAQMF
HHHHHHHHHHHHHHHCCCEEEEEEECEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHH
EGKADWSRIGELKSALSIPVIGSGDLFSAADAVGMLAETGCDSVMVARGAMGNPWIFREA
CCCCHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCHHEECCCCCCCCHHHHHH
LSLLAGEEPAPPTVQERLAVSRRHLELFAEFAGGRVALMEMRKHLSWYSKGLPGAAQFRA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
AVNRIESAPELIRAMEEFFDA
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKTVALGPLVLKNQLFLAPMAGITNLPMRIISREGGASFAFTEMVSVNGLTREGQKSFD
CCCCCCCCHHHHHCCHHHHHHCCHHHCCHHHHCCCCCCCCHHHHHHHHCCCCCCCHHHHH
LLKSSPEDRPIGMQLFGDDPEMLAEAARLVEEYGELIDINMGCPVRKVVGTGAGSALMKD
HHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCEEECCCCCCHHHHHCCCCCHHHHCC
PQKVGRIVRSVRAATKLPLTIKIRTGWVCGDDTFLEVGRIAQEEGCDAVTLHPRSRAQMF
HHHHHHHHHHHHHHHCCCEEEEEEECEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHH
EGKADWSRIGELKSALSIPVIGSGDLFSAADAVGMLAETGCDSVMVARGAMGNPWIFREA
CCCCHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHCCCCHHEECCCCCCCCHHHHHH
LSLLAGEEPAPPTVQERLAVSRRHLELFAEFAGGRVALMEMRKHLSWYSKGLPGAAQFRA
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHHHH
AVNRIESAPELIRAMEEFFDA
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]