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The map label for this gene is leuA [H]

Identifier: 197119115

GI number: 197119115

Start: 3170244

End: 3171791

Strand: Reverse

Name: leuA [H]

Synonym: Gbem_2742

Alternate gene names: 197119115

Gene position: 3171791-3170244 (Counterclockwise)

Preceding gene: 197119116

Following gene: 197119114

Centisome position: 68.73

GC content: 61.56

Gene sequence:

>1548_bases
GTGAACAACACGGAACCAAGAACCATCAAGATCTTCGATACCACGCTCAGAGACGGGGAGCAGTCCCCAGGCGCCAGCAT
GAACATCGAGGAGAAACTTCGCCTCGCCAAGCAGCTGCAGAAACTGAACGTTGACGTCATCGAGGCCGGGTTCCCTATTG
CCTCCGAGGGTGATTTCGAGGCGGTCAAGAAGGTGGCCCAGAACATCAAGGGACCCGAAATCGCCGGCCTGTGCCGCTCC
GGGTTCAAGGACATCGACCGCGCCTGGGAGGCGCTCAAGTACGCCGAGGAGAAGGGGCGCATCCACACCTTCATCGCGAC
CTCCGACATCCACATGAAGTACAAGCTGCAGATGACGCCGGCGCAGGTGTTGGAGAGCGCCGTCAAGGGTGTGAAAAGGG
CACGTTCCTATACCGCCAACGTTGAGTTCTCCTGCGAGGACGCCGTGCGCACCGATCTTAAGTTCCTCGCCGAAGTGGTC
GGCGCGGTGATCGACGCCGGGGCCTCGGTGGTCAACATCCCCGACACGGTCGGCTACACCATACCCTTCGAGTACTTCAA
CATCATCAAGTACTTGAAGGACAACGTTAAAAACATCGACGACGCCATCATCTCGGTGCACTGCCACAACGACCTGGGGC
TCTCCGTCGCCAACTCCATCGCCGCGGTCCAGGCGGGGGCGGGGCAGGTGGAGTGCACCATAAACGGCATCGGCGAGCGC
GCCGGCAACTGCTCTTTGGAAGAGTTCGTCATGGCGATCCGGACCCGCGGCGACATCCTTCCCTTCAAGACCAACGTCGT
CACCGACCAACTCACCCCGGCAAGCCGCCTTTTGACCCAGGTCACCGGGATCGTGGTGCAGCAGAACAAGGCCATCGTCG
GCGCCAACGCCTTCGCCCACGAGGCGGGGATCCACCAGCACGGCATGCTGATGGAGAAGTCCACCTACGAGATCATGACT
CCCGAGTCGGTTGGCCTCAAGTCGAGCGCCCTGGTGCTCGGCAAGCACTCCGGTCGCCACGCCTTCAAAAAGAGGCTTGA
AGAGCTCGGTCACGAGCTGGACGATGAGGCGTTGAACAAGGCGTTCACCAGGTTCAAGGCCCTTGCCGACCTGAAGAAGG
AGGTCTTCGACGAGGACCTGGACGCCATCGTGCTGGACGAGGCGAAGAACATCGAGGAGAAGTACAAGCTCTCCCACATC
ACCGTCACCTGCGGATCCTTCGCCGTCGCCACCGCGACGGTGCAGATGGAGATCGACGGCATGCAGGTGCGCGCGGCGGA
ACTCGGCGACGGGCCGGTAGACGCGACCATGAAGGCGATCAAGAAGCTCACCAAGCGCGACGTGAAGCTCTTGCAGTACA
ACGTCGGCGCCATCACCGGAGGCACCGATGCCATCGGCGAGGTCACGGTGCGCATCACCGAAGAGGACCGGACCCCAGTG
GTCGGCCGCGGCTCTTCGACCGACATCATCGAGGCTTCGGCAAAGGCTTACATCGACGCGCTGAACCGGCTCTACTTCGC
CACCGACCGCGATCTGGAAACGCTGTAA

Upstream 100 bases:

>100_bases
CTCGCGGAACCTCTCCTGGCTTTTGCCGGGAACCTCGCTTTGACCGGCAGACAGGGGAGGGAAATTTGATGCAGCCCAAC
TTCACACAGGAGGAGCGCAC

Downstream 100 bases:

>100_bases
AATAGCAGACACAGGCAGAGACAAGAAAGGAAGTCCCGCTCAGGGGCTTCCTTTTTTTGTTGTTAATTGTCAGCTGCCTG
TGTAATGCTACAGGCGTTTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MNNTEPRTIKIFDTTLRDGEQSPGASMNIEEKLRLAKQLQKLNVDVIEAGFPIASEGDFEAVKKVAQNIKGPEIAGLCRS
GFKDIDRAWEALKYAEEKGRIHTFIATSDIHMKYKLQMTPAQVLESAVKGVKRARSYTANVEFSCEDAVRTDLKFLAEVV
GAVIDAGASVVNIPDTVGYTIPFEYFNIIKYLKDNVKNIDDAIISVHCHNDLGLSVANSIAAVQAGAGQVECTINGIGER
AGNCSLEEFVMAIRTRGDILPFKTNVVTDQLTPASRLLTQVTGIVVQQNKAIVGANAFAHEAGIHQHGMLMEKSTYEIMT
PESVGLKSSALVLGKHSGRHAFKKRLEELGHELDDEALNKAFTRFKALADLKKEVFDEDLDAIVLDEAKNIEEKYKLSHI
TVTCGSFAVATATVQMEIDGMQVRAAELGDGPVDATMKAIKKLTKRDVKLLQYNVGAITGGTDAIGEVTVRITEEDRTPV
VGRGSSTDIIEASAKAYIDALNRLYFATDRDLETL

Sequences:

>Translated_515_residues
MNNTEPRTIKIFDTTLRDGEQSPGASMNIEEKLRLAKQLQKLNVDVIEAGFPIASEGDFEAVKKVAQNIKGPEIAGLCRS
GFKDIDRAWEALKYAEEKGRIHTFIATSDIHMKYKLQMTPAQVLESAVKGVKRARSYTANVEFSCEDAVRTDLKFLAEVV
GAVIDAGASVVNIPDTVGYTIPFEYFNIIKYLKDNVKNIDDAIISVHCHNDLGLSVANSIAAVQAGAGQVECTINGIGER
AGNCSLEEFVMAIRTRGDILPFKTNVVTDQLTPASRLLTQVTGIVVQQNKAIVGANAFAHEAGIHQHGMLMEKSTYEIMT
PESVGLKSSALVLGKHSGRHAFKKRLEELGHELDDEALNKAFTRFKALADLKKEVFDEDLDAIVLDEAKNIEEKYKLSHI
TVTCGSFAVATATVQMEIDGMQVRAAELGDGPVDATMKAIKKLTKRDVKLLQYNVGAITGGTDAIGEVTVRITEEDRTPV
VGRGSSTDIIEASAKAYIDALNRLYFATDRDLETL
>Mature_515_residues
MNNTEPRTIKIFDTTLRDGEQSPGASMNIEEKLRLAKQLQKLNVDVIEAGFPIASEGDFEAVKKVAQNIKGPEIAGLCRS
GFKDIDRAWEALKYAEEKGRIHTFIATSDIHMKYKLQMTPAQVLESAVKGVKRARSYTANVEFSCEDAVRTDLKFLAEVV
GAVIDAGASVVNIPDTVGYTIPFEYFNIIKYLKDNVKNIDDAIISVHCHNDLGLSVANSIAAVQAGAGQVECTINGIGER
AGNCSLEEFVMAIRTRGDILPFKTNVVTDQLTPASRLLTQVTGIVVQQNKAIVGANAFAHEAGIHQHGMLMEKSTYEIMT
PESVGLKSSALVLGKHSGRHAFKKRLEELGHELDDEALNKAFTRFKALADLKKEVFDEDLDAIVLDEAKNIEEKYKLSHI
TVTCGSFAVATATVQMEIDGMQVRAAELGDGPVDATMKAIKKLTKRDVKLLQYNVGAITGGTDAIGEVTVRITEEDRTPV
VGRGSSTDIIEASAKAYIDALNRLYFATDRDLETL

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=509, Percent_Identity=48.9194499017682, Blast_Score=461, Evalue=1e-131,
Organism=Saccharomyces cerevisiae, GI6320071, Length=386, Percent_Identity=29.7927461139896, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320019, Length=382, Percent_Identity=30.3664921465969, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324225, Length=554, Percent_Identity=24.7292418772563, Blast_Score=123, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6324682, Length=572, Percent_Identity=24.4755244755245, Blast_Score=119, Evalue=9e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56195; Mature: 56195

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNTEPRTIKIFDTTLRDGEQSPGASMNIEEKLRLAKQLQKLNVDVIEAGFPIASEGDFE
CCCCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHH
AVKKVAQNIKGPEIAGLCRSGFKDIDRAWEALKYAEEKGRIHTFIATSDIHMKYKLQMTP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEEEECCH
AQVLESAVKGVKRARSYTANVEFSCEDAVRTDLKFLAEVVGAVIDAGASVVNIPDTVGYT
HHHHHHHHHHHHHHHHEECCEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCE
IPFEYFNIIKYLKDNVKNIDDAIISVHCHNDLGLSVANSIAAVQAGAGQVECTINGIGER
ECHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCC
AGNCSLEEFVMAIRTRGDILPFKTNVVTDQLTPASRLLTQVTGIVVQQNKAIVGANAFAH
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCEEEECCHHHH
EAGIHQHGMLMEKSTYEIMTPESVGLKSSALVLGKHSGRHAFKKRLEELGHELDDEALNK
HCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHH
AFTRFKALADLKKEVFDEDLDAIVLDEAKNIEEKYKLSHITVTCGSFAVATATVQMEIDG
HHHHHHHHHHHHHHHHHCCCCEEEECCHHCHHHHHEEEEEEEEECCEEEEEEEEEEEECC
MQVRAAELGDGPVDATMKAIKKLTKRDVKLLQYNVGAITGGTDAIGEVTVRITEEDRTPV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCEECCCCCCCCEEEEEEECCCCCCE
VGRGSSTDIIEASAKAYIDALNRLYFATDRDLETL
EECCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCC
>Mature Secondary Structure
MNNTEPRTIKIFDTTLRDGEQSPGASMNIEEKLRLAKQLQKLNVDVIEAGFPIASEGDFE
CCCCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHH
AVKKVAQNIKGPEIAGLCRSGFKDIDRAWEALKYAEEKGRIHTFIATSDIHMKYKLQMTP
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEEEECCH
AQVLESAVKGVKRARSYTANVEFSCEDAVRTDLKFLAEVVGAVIDAGASVVNIPDTVGYT
HHHHHHHHHHHHHHHHEECCEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCE
IPFEYFNIIKYLKDNVKNIDDAIISVHCHNDLGLSVANSIAAVQAGAGQVECTINGIGER
ECHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEEEECCCCC
AGNCSLEEFVMAIRTRGDILPFKTNVVTDQLTPASRLLTQVTGIVVQQNKAIVGANAFAH
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCEEEECCHHHH
EAGIHQHGMLMEKSTYEIMTPESVGLKSSALVLGKHSGRHAFKKRLEELGHELDDEALNK
HCCCCCCCEEEECCCEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHH
AFTRFKALADLKKEVFDEDLDAIVLDEAKNIEEKYKLSHITVTCGSFAVATATVQMEIDG
HHHHHHHHHHHHHHHHHCCCCEEEECCHHCHHHHHEEEEEEEEECCEEEEEEEEEEEECC
MQVRAAELGDGPVDATMKAIKKLTKRDVKLLQYNVGAITGGTDAIGEVTVRITEEDRTPV
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHEEECCEECCCCCCCCEEEEEEECCCCCCE
VGRGSSTDIIEASAKAYIDALNRLYFATDRDLETL
EECCCCCCHHHHHHHHHHHHHHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA