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The map label for this gene is leuC

Identifier: 197119110

GI number: 197119110

Start: 3164616

End: 3165899

Strand: Reverse

Name: leuC

Synonym: Gbem_2737

Alternate gene names: 197119110

Gene position: 3165899-3164616 (Counterclockwise)

Preceding gene: 197119113

Following gene: 197119109

Centisome position: 68.6

GC content: 63.01

Gene sequence:

>1284_bases
ATGGGTATGACTACGGCTCAAAAAATATTTTCGGCCCATCTGGTTGATGAGCCTTTTGCTGGCACCAAGGTGCTCAGCAT
CGACGTCGTGATGTGCCACGAGATCACGACCCCGATCGCCATCGCCGATCTGATGGCGCGCGGTAAAGACAGGGTTTTCG
ACCCCACCAAGATCAAGGCGGTCATCGACCACGTAACGCCGAGCAAGGACAGCAAGACCGCCACCCAGGCGAAGATGCTG
CGCGACTGGGCCAGGCGGCACGACATCAAGGACTTCTTCGACATCGGGGCCAACGGCGTCTGCCACGCGCTCTTCCCGGA
GAAGGGTTTCATCCGCCCGGGTAACACGGTGATCATGGGCGACTCCCACACCTGCACCCACGGCGCCTTCGGGGCCTTCG
CCGCCGGCGTCGGCACCACCGACCTCGAAGTAGGGATCCTCAAGGGGGTCTGCGCCTTCCGCGAGCCCAAGACCATCCGC
GTCAACCTGAACGGCACCCTCCCCAAAGGGGTTTTCGCCAAGGACGCCATCCTGCGCGTGATCGGGCACCTGGGTGTTAA
CGGCGCCACCGACCGCGTCATCGAGTTCGGCGGGCCGGTCGTGGCCCAGATGACCATGGAGTCGAGGATGACGCTTTGCA
ACATGGCGATCGAGGCGGGGGGCACCTCCGGCATCTGCATGCCGGACCAGGTCACCGTCGATTACCTCTGGCCCTTCATC
TCCGGATCGTTCGGATCGAAGGAAGAGGCGCTTGCCGCCTACAGCGTCTGGTGCTCGGACGCCGACGCCGCTTACGAGCA
GGTGATCGATCTCGATCTTTCCGACCTCGCCCCGCTTTGCACCTTCGGCTACAAGCCGGACCAGGTGAAGAGCGTGACCG
AGATGGCCGGCACCCAGGTGGACCAGGTTTACCTCGGATCTTGCACCAACGGCCGGCTGGAAGACCTCAGGGTTGCAGCC
CAGATCCTCAAGGGGAAGAAGATCGCCTCCCACGTGCGCGCCATCCTTTCTCCGGCAACGCCGCAGATCTACAAGGATGC
GGTTGCCGAAGGGCTGATCCAGATCTTCATGGACGCAGGCTTCTGCGTCACCAACCCGACCTGCGGCGCCTGCCTCGGCA
TGAGTAACGGCGTCCTCGCCGAAGGCGAAGTCTGCGCCTCCACCACCAACCGCAACTTCATGGGGAGGATGGGCAAGGGG
GGGATGGTGCATCTCCTCTCTCCGGCGACGGCAGCTGCCTCCGCCATCGAGGGTAAGATCGCCGATCCGCGCAACTACTT
GTAA

Upstream 100 bases:

>100_bases
TAATAACATTGAACAATTATTAGTTTGATTTGTAGGGCATATTTTATTATTGTGGCGATTGGTAAAACCTCCAATCCAAT
CGCAAAAGGAAGCGCTATTT

Downstream 100 bases:

>100_bases
CCAATTGACAATTGATAATTGACAATTGACGATGAACGCCCAACCGTTACCGGCAGCGTTACTCATGATGCGAACTTCGA
CTTTCAGGAGATGAGATATG

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MGMTTAQKIFSAHLVDEPFAGTKVLSIDVVMCHEITTPIAIADLMARGKDRVFDPTKIKAVIDHVTPSKDSKTATQAKML
RDWARRHDIKDFFDIGANGVCHALFPEKGFIRPGNTVIMGDSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFREPKTIR
VNLNGTLPKGVFAKDAILRVIGHLGVNGATDRVIEFGGPVVAQMTMESRMTLCNMAIEAGGTSGICMPDQVTVDYLWPFI
SGSFGSKEEALAAYSVWCSDADAAYEQVIDLDLSDLAPLCTFGYKPDQVKSVTEMAGTQVDQVYLGSCTNGRLEDLRVAA
QILKGKKIASHVRAILSPATPQIYKDAVAEGLIQIFMDAGFCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKG
GMVHLLSPATAAASAIEGKIADPRNYL

Sequences:

>Translated_427_residues
MGMTTAQKIFSAHLVDEPFAGTKVLSIDVVMCHEITTPIAIADLMARGKDRVFDPTKIKAVIDHVTPSKDSKTATQAKML
RDWARRHDIKDFFDIGANGVCHALFPEKGFIRPGNTVIMGDSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFREPKTIR
VNLNGTLPKGVFAKDAILRVIGHLGVNGATDRVIEFGGPVVAQMTMESRMTLCNMAIEAGGTSGICMPDQVTVDYLWPFI
SGSFGSKEEALAAYSVWCSDADAAYEQVIDLDLSDLAPLCTFGYKPDQVKSVTEMAGTQVDQVYLGSCTNGRLEDLRVAA
QILKGKKIASHVRAILSPATPQIYKDAVAEGLIQIFMDAGFCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKG
GMVHLLSPATAAASAIEGKIADPRNYL
>Mature_426_residues
GMTTAQKIFSAHLVDEPFAGTKVLSIDVVMCHEITTPIAIADLMARGKDRVFDPTKIKAVIDHVTPSKDSKTATQAKMLR
DWARRHDIKDFFDIGANGVCHALFPEKGFIRPGNTVIMGDSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFREPKTIRV
NLNGTLPKGVFAKDAILRVIGHLGVNGATDRVIEFGGPVVAQMTMESRMTLCNMAIEAGGTSGICMPDQVTVDYLWPFIS
GSFGSKEEALAAYSVWCSDADAAYEQVIDLDLSDLAPLCTFGYKPDQVKSVTEMAGTQVDQVYLGSCTNGRLEDLRVAAQ
ILKGKKIASHVRAILSPATPQIYKDAVAEGLIQIFMDAGFCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKGG
MVHLLSPATAAASAIEGKIADPRNYL

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=454, Percent_Identity=27.7533039647577, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI8659555, Length=464, Percent_Identity=24.1379310344828, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI41352693, Length=389, Percent_Identity=24.4215938303342, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=37.6344086021505, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI2367097, Length=362, Percent_Identity=25.1381215469613, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87081781, Length=346, Percent_Identity=23.6994219653179, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787531, Length=362, Percent_Identity=24.8618784530387, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=457, Percent_Identity=27.7899343544858, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=346, Percent_Identity=29.7687861271676, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI25149342, Length=281, Percent_Identity=29.8932384341637, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=36.0934182590234, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6320440, Length=456, Percent_Identity=30.7017543859649, Blast_Score=182, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6323335, Length=453, Percent_Identity=26.9315673289183, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=457, Percent_Identity=27.1334792122538, Blast_Score=133, Evalue=5e-32,
Organism=Drosophila melanogaster, GI281365315, Length=457, Percent_Identity=26.0393873085339, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17864292, Length=457, Percent_Identity=26.0393873085339, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI28571643, Length=455, Percent_Identity=26.1538461538462, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161076999, Length=420, Percent_Identity=25.7142857142857, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24645686, Length=372, Percent_Identity=26.0752688172043, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=24.7978436657682, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_GEOBB (B5EHU2)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002139537.1
- GeneID:   6782735
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_2737
- HOGENOM:   HBG330745
- OMA:   RQGIEHV
- ProtClustDB:   PRK00402
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 45450; Mature: 45319

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMTTAQKIFSAHLVDEPFAGTKVLSIDVVMCHEITTPIAIADLMARGKDRVFDPTKIKA
CCCCHHHHHHHHHHCCCCCCCCEEEEEEEEEEHHCCCHHHHHHHHHCCCCCCCCHHHHHH
VIDHVTPSKDSKTATQAKMLRDWARRHDIKDFFDIGANGVCHALFPEKGFIRPGNTVIMG
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCCCCCCCCEEEEC
DSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFREPKTIRVNLNGTLPKGVFAKDAILRV
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHH
IGHLGVNGATDRVIEFGGPVVAQMTMESRMTLCNMAIEAGGTSGICMPDQVTVDYLWPFI
HHHCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
SGSFGSKEEALAAYSVWCSDADAAYEQVIDLDLSDLAPLCTFGYKPDQVKSVTEMAGTQV
CCCCCCHHHHHHHHHEECCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCC
DQVYLGSCTNGRLEDLRVAAQILKGKKIASHVRAILSPATPQIYKDAVAEGLIQIFMDAG
CEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
FCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKGGMVHLLSPATAAASAIEGKI
CEECCCCCHHHHCCCCCCEECCCEEECCCCCCHHCCCCCCCEEEEECCHHHHHHHHCCCC
ADPRNYL
CCCCCCC
>Mature Secondary Structure 
GMTTAQKIFSAHLVDEPFAGTKVLSIDVVMCHEITTPIAIADLMARGKDRVFDPTKIKA
CCCHHHHHHHHHHCCCCCCCCEEEEEEEEEEHHCCCHHHHHHHHHCCCCCCCCHHHHHH
VIDHVTPSKDSKTATQAKMLRDWARRHDIKDFFDIGANGVCHALFPEKGFIRPGNTVIMG
HHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCEEEEECCCCCCCCCCCEEEEC
DSHTCTHGAFGAFAAGVGTTDLEVGILKGVCAFREPKTIRVNLNGTLPKGVFAKDAILRV
CCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHH
IGHLGVNGATDRVIEFGGPVVAQMTMESRMTLCNMAIEAGGTSGICMPDQVTVDYLWPFI
HHHCCCCCCHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
SGSFGSKEEALAAYSVWCSDADAAYEQVIDLDLSDLAPLCTFGYKPDQVKSVTEMAGTQV
CCCCCCHHHHHHHHHEECCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCC
DQVYLGSCTNGRLEDLRVAAQILKGKKIASHVRAILSPATPQIYKDAVAEGLIQIFMDAG
CEEEECCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
FCVTNPTCGACLGMSNGVLAEGEVCASTTNRNFMGRMGKGGMVHLLSPATAAASAIEGKI
CEECCCCCHHHHCCCCCCEECCCEEECCCCCCHHCCCCCCCEEEEECCHHHHHHHHCCCC
ADPRNYL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA