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The map label for this gene is leuD [H]

Identifier: 197119109

GI number: 197119109

Start: 3163991

End: 3164518

Strand: Reverse

Name: leuD [H]

Synonym: Gbem_2736

Alternate gene names: 197119109

Gene position: 3164518-3163991 (Counterclockwise)

Preceding gene: 197119110

Following gene: 197119108

Centisome position: 68.57

GC content: 59.47

Gene sequence:

>528_bases
ATGAAAAAATTTGGCGGCCCGGCCCTCTTTCTGGACCGTAGCGACATAAACACCGACGAGATCATCCCGGCAAAATACCT
GACGGAGATCACCAAGGAAGACCTGAAGCCCTACCTGCTCGAAGACCTGAAGCTCCCCGGCTTCGACGCCAAGTCTGAGA
AGACCTATGGCGCAGGCGTCGTGGTGACCCGCGCCAACTTCGGCTGCGGCTCCTCCCGCGAGCACGCACCCTGGGTCTTC
GAGGTGAACGGCATCAACGTGGTCCTGGCGCAGTCCTTCGCCAGGATCTTCAGGCAGAACATGTTCAACTGCGGCATGGT
GGCCATCGAACTCTCAGCTGAGCAGATCGACCGCCTCTTCGGCTTTGCCGGCGCAGCAGACGCCACCGTGGACGTAGACG
TGGAAAACGGCACTCTCACTATCAAGGGGGGCGGGCGTCAGGAGTCTATGCAGTTCGAGATCTCTCCCTTTGACAAAGCT
CTGGTTCTGGCCGGCGGCTGGGTGGATTACGCCGACAGCAAATACTAG

Upstream 100 bases:

>100_bases
TAACCAATTGACAATTGATAATTGACAATTGACGATGAACGCCCAACCGTTACCGGCAGCGTTACTCATGATGCGAACTT
CGACTTTCAGGAGATGAGAT

Downstream 100 bases:

>100_bases
CAGCAGGGCACTAACAGCGTCCAAAAACAATGAGGCAGGAGACCCGACGGTTTCCTGCCTCATTGTTTTCCTGTCGTCCT
TGATTGACAGTCGCGACTTT

Product: isopropylmalate/citramalate isomerase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKKFGGPALFLDRSDINTDEIIPAKYLTEITKEDLKPYLLEDLKLPGFDAKSEKTYGAGVVVTRANFGCGSSREHAPWVF
EVNGINVVLAQSFARIFRQNMFNCGMVAIELSAEQIDRLFGFAGAADATVDVDVENGTLTIKGGGRQESMQFEISPFDKA
LVLAGGWVDYADSKY

Sequences:

>Translated_175_residues
MKKFGGPALFLDRSDINTDEIIPAKYLTEITKEDLKPYLLEDLKLPGFDAKSEKTYGAGVVVTRANFGCGSSREHAPWVF
EVNGINVVLAQSFARIFRQNMFNCGMVAIELSAEQIDRLFGFAGAADATVDVDVENGTLTIKGGGRQESMQFEISPFDKA
LVLAGGWVDYADSKY
>Mature_175_residues
MKKFGGPALFLDRSDINTDEIIPAKYLTEITKEDLKPYLLEDLKLPGFDAKSEKTYGAGVVVTRANFGCGSSREHAPWVF
EVNGINVVLAQSFARIFRQNMFNCGMVAIELSAEQIDRLFGFAGAADATVDVDVENGTLTIKGGGRQESMQFEISPFDKA
LVLAGGWVDYADSKY

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=172, Percent_Identity=33.1395348837209, Blast_Score=101, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=167, Percent_Identity=34.1317365269461, Blast_Score=82, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320440, Length=115, Percent_Identity=34.7826086956522, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 19187; Mature: 19187

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFGGPALFLDRSDINTDEIIPAKYLTEITKEDLKPYLLEDLKLPGFDAKSEKTYGAGV
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEE
VVTRANFGCGSSREHAPWVFEVNGINVVLAQSFARIFRQNMFNCGMVAIELSAEQIDRLF
EEEECCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHH
GFAGAADATVDVDVENGTLTIKGGGRQESMQFEISPFDKALVLAGGWVDYADSKY
CCCCCCCCEEEEEECCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MKKFGGPALFLDRSDINTDEIIPAKYLTEITKEDLKPYLLEDLKLPGFDAKSEKTYGAGV
CCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCEE
VVTRANFGCGSSREHAPWVFEVNGINVVLAQSFARIFRQNMFNCGMVAIELSAEQIDRLF
EEEECCCCCCCCCCCCCEEEEECCCEEEHHHHHHHHHHHCCCCCCEEEEEECHHHHHHHH
GFAGAADATVDVDVENGTLTIKGGGRQESMQFEISPFDKALVLAGGWVDYADSKY
CCCCCCCCEEEEEECCCEEEEECCCCCCCEEEEECCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]