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The map label for this gene is rpoS [H]

Identifier: 197119058

GI number: 197119058

Start: 3106852

End: 3107853

Strand: Reverse

Name: rpoS [H]

Synonym: Gbem_2683

Alternate gene names: 197119058

Gene position: 3107853-3106852 (Counterclockwise)

Preceding gene: 308535315

Following gene: 197119057

Centisome position: 67.34

GC content: 59.28

Gene sequence:

>1002_bases
ATGGAAAACGAAGGACATTTAGAGGAGAAGGAACCTCTTTTTCTGGACGCCGAGCAGGACCCCGACGCACTCGAACTGGA
GGAAGTCGAGGCCGAAGCCGACGAACCTGCCGAGGTAGAGGAAGAGGAAATCAAGGCCGCAGTGGTCGAGCATTTCGATG
ACGCCATCAAGCTCTACCTGCGCGAAATCCAGAAGACCAAGCTTCTCACCGCCGACGAGGAGAAGGAGTTGGCTGCGCGT
ATCGACCTGGGCGACAAGGCCGCCCGCGACCGCATGATCGTCTCCAACCTCCGCCTGGTGGTGAAGATCGCCAAGCGCTA
CATAAACCGCGGGCTCCCCTTCCTGGATCTCATCGAAGAAGGGAACATGGGGCTCATCAAGGCCGTGGAGCGCTTCAAGC
TCTCCAAGGAGTGCCGCTTCTCCACCTACGCCACCTGGTGGATCCGGCAGTCCATCGAGCGCGCGCTGGTGAACCAGTCG
CGCACCATCCGCCTGCCGGTGCACGTCTCCGACGACATCAACAAGATGCTAAGGGTGACGCGCGAACTGGTGCAGAAGAT
GAACCGCGAGCCGAGCATCAAGGAAGTCGCCGACACCCTTGAGGTGAACGTCACCTACGTGCGCAGGCTCATGGTCCTCC
TGAAGAAGACCTACTCCATCGAGCGCCCCATGGGGGAGAACAACGACTATTTCCTTATCGACACCATAGAGGACACCTCC
ACCATATCGCCGGCCGTGCTCCTGGAGGACCTCAACAAGTACGAGCTGGTCTCCAAGTGGTTCGAGACCCTCTCCGACGC
CGAGAAGAAGATCCTCACGCTCCGTTTTGGTCTCGACGACAAGGACCCCCAGACCCTCGACACCATCGGGCGCAGCTTCG
GCGTGACCCGCGAGAGGATCAGGCAGATCGAAGCGAAATCGCTGGAAAAGCTGAGAAAGATAGTAGAAACGACCGATGTC
ATGGGGCGCGCGGCTGTCCCCCCGACAACTACAGGCACATAA

Upstream 100 bases:

>100_bases
CTGCCGCTTCGTAAGACTTATTGGAAGAAACGGCTGGAATGACGAGCAGTAGCAAAGGTTGCAGACACACCATCTCAAAG
CTTTGCTAAAGGAGTCTCGC

Downstream 100 bases:

>100_bases
GGAGCGGCAGGATAAATGGAAGATCTGAAAAGCATCATCAGGAACATCCCGGACTTCCCCAAGAAGGGGATACTTTTCAA
GGACATCACCACGCTCTTAG

Product: RNA polymerase sigma-38 factor, stationary phase

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MENEGHLEEKEPLFLDAEQDPDALELEEVEAEADEPAEVEEEEIKAAVVEHFDDAIKLYLREIQKTKLLTADEEKELAAR
IDLGDKAARDRMIVSNLRLVVKIAKRYINRGLPFLDLIEEGNMGLIKAVERFKLSKECRFSTYATWWIRQSIERALVNQS
RTIRLPVHVSDDINKMLRVTRELVQKMNREPSIKEVADTLEVNVTYVRRLMVLLKKTYSIERPMGENNDYFLIDTIEDTS
TISPAVLLEDLNKYELVSKWFETLSDAEKKILTLRFGLDDKDPQTLDTIGRSFGVTRERIRQIEAKSLEKLRKIVETTDV
MGRAAVPPTTTGT

Sequences:

>Translated_333_residues
MENEGHLEEKEPLFLDAEQDPDALELEEVEAEADEPAEVEEEEIKAAVVEHFDDAIKLYLREIQKTKLLTADEEKELAAR
IDLGDKAARDRMIVSNLRLVVKIAKRYINRGLPFLDLIEEGNMGLIKAVERFKLSKECRFSTYATWWIRQSIERALVNQS
RTIRLPVHVSDDINKMLRVTRELVQKMNREPSIKEVADTLEVNVTYVRRLMVLLKKTYSIERPMGENNDYFLIDTIEDTS
TISPAVLLEDLNKYELVSKWFETLSDAEKKILTLRFGLDDKDPQTLDTIGRSFGVTRERIRQIEAKSLEKLRKIVETTDV
MGRAAVPPTTTGT
>Mature_333_residues
MENEGHLEEKEPLFLDAEQDPDALELEEVEAEADEPAEVEEEEIKAAVVEHFDDAIKLYLREIQKTKLLTADEEKELAAR
IDLGDKAARDRMIVSNLRLVVKIAKRYINRGLPFLDLIEEGNMGLIKAVERFKLSKECRFSTYATWWIRQSIERALVNQS
RTIRLPVHVSDDINKMLRVTRELVQKMNREPSIKEVADTLEVNVTYVRRLMVLLKKTYSIERPMGENNDYFLIDTIEDTS
TISPAVLLEDLNKYELVSKWFETLSDAEKKILTLRFGLDDKDPQTLDTIGRSFGVTRERIRQIEAKSLEKLRKIVETTDV
MGRAAVPPTTTGT

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=306, Percent_Identity=45.4248366013072, Blast_Score=257, Evalue=9e-70,
Organism=Escherichia coli, GI1789448, Length=259, Percent_Identity=44.015444015444, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=29.3478260869565, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38367; Mature: 38367

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENEGHLEEKEPLFLDAEQDPDALELEEVEAEADEPAEVEEEEIKAAVVEHFDDAIKLYL
CCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
REIQKTKLLTADEEKELAARIDLGDKAARDRMIVSNLRLVVKIAKRYINRGLPFLDLIEE
HHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHC
GNMGLIKAVERFKLSKECRFSTYATWWIRQSIERALVNQSRTIRLPVHVSDDINKMLRVT
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHH
RELVQKMNREPSIKEVADTLEVNVTYVRRLMVLLKKTYSIERPMGENNDYFLIDTIEDTS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
TISPAVLLEDLNKYELVSKWFETLSDAEKKILTLRFGLDDKDPQTLDTIGRSFGVTRERI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCHHHH
RQIEAKSLEKLRKIVETTDVMGRAAVPPTTTGT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MENEGHLEEKEPLFLDAEQDPDALELEEVEAEADEPAEVEEEEIKAAVVEHFDDAIKLYL
CCCCCCCCCCCCEEEECCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
REIQKTKLLTADEEKELAARIDLGDKAARDRMIVSNLRLVVKIAKRYINRGLPFLDLIEE
HHHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHC
GNMGLIKAVERFKLSKECRFSTYATWWIRQSIERALVNQSRTIRLPVHVSDDINKMLRVT
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHH
RELVQKMNREPSIKEVADTLEVNVTYVRRLMVLLKKTYSIERPMGENNDYFLIDTIEDTS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCC
TISPAVLLEDLNKYELVSKWFETLSDAEKKILTLRFGLDDKDPQTLDTIGRSFGVTRERI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHCCCHHHH
RQIEAKSLEKLRKIVETTDVMGRAAVPPTTTGT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]