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The map label for this gene is pilS [H]

Identifier: 197118969

GI number: 197118969

Start: 3001414

End: 3003033

Strand: Reverse

Name: pilS [H]

Synonym: Gbem_2592

Alternate gene names: 197118969

Gene position: 3003033-3001414 (Counterclockwise)

Preceding gene: 197118970

Following gene: 197118968

Centisome position: 65.07

GC content: 60.86

Gene sequence:

>1620_bases
ATGATCGACAGGAAAAGGGTTTTTTGGTTCATACTGCTTAGGTTGTTGGTGGTGTCCATTTTCCTGGCCACCACCACTTT
CCTCGACGTACGTACCTATGACGTTGGTACCAACATCGCCCACAAGGTGCTGATCCGGTTAATGGGCGCCACCTATCTTT
TCTCGCTCGGCTCGTTGGTGGTCCTCTATTACTCGCGCCGGCAGATCCGCACCCTCACTTACGCTCAAATCGTCTGGGAC
CTGATCCTGGTTACGGTGATGATCCTGATCTCGGGCGGGGTCACATCGCCTTACGCCTTTCTCTATTTTTTATCGATCAT
CAGCGCCAGCACGCTTCTGGCACGCTCGCAGGCGTACTACACCGCCTCGCTCTGCGTCATCCTTTACGGTGCCATCCTCG
ACCTGCAGTACTACGGCAAGCTGGCGCCGCTAGGCTTGAGCCCTTTCCCGGCGCAGCAGTATGGCGCGCAGTACCTGTTC
TTCCTCATCTTCATGTACTGCGCTGCGTTCTTTCTCACCGCCTTGCTGGCCGGCCACCTATCGGAGCGCGCCCGGCTCTC
GGAGAGCGCACTCCAAGAAAAGGCCATCGATTACGAGGAGTTGGAACGGCTCAACTCCTTGATCGTCTCGACCATAGATT
CGGGCCTGCTCACCATAAACTCCGACGGGAGGATCCGCGTCTTCAACCGATATGCCGAGCATCTGACCGGGGTCTCGCAG
CAGGATGCCTACGACCACCCGCTTGCCGAGGTGCTGCCAGGGGTAGCGCCCTTCGCCGGGCTTTTCTTTCAGGGGGGGGA
GGGAGAGTTCACGCACAAGGGGGTCGACGGACGGGAACTGCTCTTCTCCTTCAAGTCGGTTCCGCTCACGGGCAAGGACG
GAGCTACGGTGGGGGCAATCTTCGATCTCCACGACCTGACCGAGATGAAGCGCCTAGCCGCTGGTCTGAAACGAGCCGAC
CGGCTCGCCGCCGTCGGGGAGCTTTCCGCCCGCATGGCGCACGAGATCAGAAATCCCCTTGCCGCCATCAGCGGCTCGGT
GCAGTTGGTCGCGCTCAGGCCCTGGGCCGACGAGAAGGACAAGCGCCTGTTTTCCATCATCTTGAGGGAGACCGACCGGC
TGGACGGACTCTTGCGCGACTTCCTTTTCTACGCCAAGCCAACCCAGCCCACCAAGATCCCCCTCAAGCTGCGCCGGGTC
ATCGCCGATCTCTGCTCCCTTCTATCCACCGACCCGCGCCTTAGGGGCGTAACCATAGAGAACCGGGTGCCGGAAGAGCT
GGTGGTGGTCTTTGACAAGGACCAGTGCTCGCAGGTCTTCTGGAACCTTGTGGTGAACAGCGCCGAGGCGATCGCCGGGG
AGGGGACCATCGTCATTGACGCCTGCGCCGCCAGCAACGGCGAGAAGGAAGAGGCCCGGATCAGCGTCAGAGACAGCGGC
TCCGGTATGAAACAGGCGGATGTGAAGCGGGTCTTCGAGCCCTTCTTCACCACAAAAAAGGGAGGGACCGGCCTCGGGCT
AGCCACCGTCTACCGCATCGTGGAGACCCACGGAGGGCGGATGGTGGTTGACAGCGCGGAAGGGGCCGGGACCACGGTGA
CCCTCTACCTTCCGACGTGA

Upstream 100 bases:

>100_bases
TTGTTGATGGTGTTCCTGGGAACCACGGTTGGCGGCCTGGTCGTCGCGATGTACCTGCCGATCTTCAAGCTTGCCGGCGC
GGTCGGCGGCGGTTGATTGG

Downstream 100 bases:

>100_bases
GTGGACGTGGGCGGTCAAGGCCGGAATATGAGTGACGGTAAGAAACCACTGGTTGCCGCAGTAAACGGGCGACCCTTTTT
GTCTGAGAGGTGACGCGATG

Product: PAS domain-containing sensor histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MIDRKRVFWFILLRLLVVSIFLATTTFLDVRTYDVGTNIAHKVLIRLMGATYLFSLGSLVVLYYSRRQIRTLTYAQIVWD
LILVTVMILISGGVTSPYAFLYFLSIISASTLLARSQAYYTASLCVILYGAILDLQYYGKLAPLGLSPFPAQQYGAQYLF
FLIFMYCAAFFLTALLAGHLSERARLSESALQEKAIDYEELERLNSLIVSTIDSGLLTINSDGRIRVFNRYAEHLTGVSQ
QDAYDHPLAEVLPGVAPFAGLFFQGGEGEFTHKGVDGRELLFSFKSVPLTGKDGATVGAIFDLHDLTEMKRLAAGLKRAD
RLAAVGELSARMAHEIRNPLAAISGSVQLVALRPWADEKDKRLFSIILRETDRLDGLLRDFLFYAKPTQPTKIPLKLRRV
IADLCSLLSTDPRLRGVTIENRVPEELVVVFDKDQCSQVFWNLVVNSAEAIAGEGTIVIDACAASNGEKEEARISVRDSG
SGMKQADVKRVFEPFFTTKKGGTGLGLATVYRIVETHGGRMVVDSAEGAGTTVTLYLPT

Sequences:

>Translated_539_residues
MIDRKRVFWFILLRLLVVSIFLATTTFLDVRTYDVGTNIAHKVLIRLMGATYLFSLGSLVVLYYSRRQIRTLTYAQIVWD
LILVTVMILISGGVTSPYAFLYFLSIISASTLLARSQAYYTASLCVILYGAILDLQYYGKLAPLGLSPFPAQQYGAQYLF
FLIFMYCAAFFLTALLAGHLSERARLSESALQEKAIDYEELERLNSLIVSTIDSGLLTINSDGRIRVFNRYAEHLTGVSQ
QDAYDHPLAEVLPGVAPFAGLFFQGGEGEFTHKGVDGRELLFSFKSVPLTGKDGATVGAIFDLHDLTEMKRLAAGLKRAD
RLAAVGELSARMAHEIRNPLAAISGSVQLVALRPWADEKDKRLFSIILRETDRLDGLLRDFLFYAKPTQPTKIPLKLRRV
IADLCSLLSTDPRLRGVTIENRVPEELVVVFDKDQCSQVFWNLVVNSAEAIAGEGTIVIDACAASNGEKEEARISVRDSG
SGMKQADVKRVFEPFFTTKKGGTGLGLATVYRIVETHGGRMVVDSAEGAGTTVTLYLPT
>Mature_539_residues
MIDRKRVFWFILLRLLVVSIFLATTTFLDVRTYDVGTNIAHKVLIRLMGATYLFSLGSLVVLYYSRRQIRTLTYAQIVWD
LILVTVMILISGGVTSPYAFLYFLSIISASTLLARSQAYYTASLCVILYGAILDLQYYGKLAPLGLSPFPAQQYGAQYLF
FLIFMYCAAFFLTALLAGHLSERARLSESALQEKAIDYEELERLNSLIVSTIDSGLLTINSDGRIRVFNRYAEHLTGVSQ
QDAYDHPLAEVLPGVAPFAGLFFQGGEGEFTHKGVDGRELLFSFKSVPLTGKDGATVGAIFDLHDLTEMKRLAAGLKRAD
RLAAVGELSARMAHEIRNPLAAISGSVQLVALRPWADEKDKRLFSIILRETDRLDGLLRDFLFYAKPTQPTKIPLKLRRV
IADLCSLLSTDPRLRGVTIENRVPEELVVVFDKDQCSQVFWNLVVNSAEAIAGEGTIVIDACAASNGEKEEARISVRDSG
SGMKQADVKRVFEPFFTTKKGGTGLGLATVYRIVETHGGRMVVDSAEGAGTTVTLYLPT

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=346, Percent_Identity=30.635838150289, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=39.2070484581498, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1790300, Length=350, Percent_Identity=28, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI48994928, Length=362, Percent_Identity=25.9668508287293, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1786600, Length=248, Percent_Identity=26.6129032258064, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI87081816, Length=222, Percent_Identity=29.7297297297297, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789149, Length=232, Percent_Identity=28.0172413793103, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1788393, Length=240, Percent_Identity=25.4166666666667, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789808, Length=235, Percent_Identity=25.9574468085106, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788713, Length=352, Percent_Identity=22.7272727272727, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI145693157, Length=259, Percent_Identity=26.6409266409266, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 59566; Mature: 59566

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRKRVFWFILLRLLVVSIFLATTTFLDVRTYDVGTNIAHKVLIRLMGATYLFSLGSLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
VLYYSRRQIRTLTYAQIVWDLILVTVMILISGGVTSPYAFLYFLSIISASTLLARSQAYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TASLCVILYGAILDLQYYGKLAPLGLSPFPAQQYGAQYLFFLIFMYCAAFFLTALLAGHL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SERARLSESALQEKAIDYEELERLNSLIVSTIDSGLLTINSDGRIRVFNRYAEHLTGVSQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHCCCCC
QDAYDHPLAEVLPGVAPFAGLFFQGGEGEFTHKGVDGRELLFSFKSVPLTGKDGATVGAI
CCCCCCCHHHHCCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEHH
FDLHDLTEMKRLAAGLKRADRLAAVGELSARMAHEIRNPLAAISGSVQLVALRPWADEKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEEECCCCCCHH
KRLFSIILRETDRLDGLLRDFLFYAKPTQPTKIPLKLRRVIADLCSLLSTDPRLRGVTIE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECEEEC
NRVPEELVVVFDKDQCSQVFWNLVVNSAEAIAGEGTIVIDACAASNGEKEEARISVRDSG
CCCCCCEEEEECCHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCC
SGMKQADVKRVFEPFFTTKKGGTGLGLATVYRIVETHGGRMVVDSAEGAGTTVTLYLPT
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECC
>Mature Secondary Structure
MIDRKRVFWFILLRLLVVSIFLATTTFLDVRTYDVGTNIAHKVLIRLMGATYLFSLGSLV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
VLYYSRRQIRTLTYAQIVWDLILVTVMILISGGVTSPYAFLYFLSIISASTLLARSQAYY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TASLCVILYGAILDLQYYGKLAPLGLSPFPAQQYGAQYLFFLIFMYCAAFFLTALLAGHL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SERARLSESALQEKAIDYEELERLNSLIVSTIDSGLLTINSDGRIRVFNRYAEHLTGVSQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHCCCCC
QDAYDHPLAEVLPGVAPFAGLFFQGGEGEFTHKGVDGRELLFSFKSVPLTGKDGATVGAI
CCCCCCCHHHHCCCHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEHH
FDLHDLTEMKRLAAGLKRADRLAAVGELSARMAHEIRNPLAAISGSVQLVALRPWADEKD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEEECCCCCCHH
KRLFSIILRETDRLDGLLRDFLFYAKPTQPTKIPLKLRRVIADLCSLLSTDPRLRGVTIE
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECEEEC
NRVPEELVVVFDKDQCSQVFWNLVVNSAEAIAGEGTIVIDACAASNGEKEEARISVRDSG
CCCCCCEEEEECCHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCC
SGMKQADVKRVFEPFFTTKKGGTGLGLATVYRIVETHGGRMVVDSAEGAGTTVTLYLPT
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]