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The map label for this gene is pilR [H]

Identifier: 197118968

GI number: 197118968

Start: 2999943

End: 3001316

Strand: Reverse

Name: pilR [H]

Synonym: Gbem_2591

Alternate gene names: 197118968

Gene position: 3001316-2999943 (Counterclockwise)

Preceding gene: 197118969

Following gene: 197118967

Centisome position: 65.03

GC content: 63.32

Gene sequence:

>1374_bases
ATGCGCGCCAGAATTTTGGTAGTAGATGATGAACTGAGCATGCGGGAGTTTCTCTCCATCCTGCTGGAGGGGGAGGGGTA
CCTGGTCGACCAGGCGGAGAACGCCGAGGACGCGCTGCGCTGCATGGGGGAGCAGCGCTACGAGATGGTGATCTCCGACG
TCCTAATGCCGGGATTGGGGGGGATCGAACTCCTTTCCAGGATCAAGAGCGAGTCCCCCGAGACAGCGGTACTGATGATC
ACCGCCTTCACCACAGCCGAACAGGCGGTCGAGGCGATGAAACTGGGCGCCTACGACTACATAGGCAAACCCTTCAAGGT
GGAAGAGGTGAAGGTGCTGGTCAGGAACGCGTTGGAAAAGCAAAGCCTGGTCCAGGAGAACAAGCGGCTGAAGGCCGAGG
TGCAGGAACGTTTCAGCTTTTCCGGCCTGATCGGCAAGAGCAAGCAGATGCGGGAGGTGTACGACCTGATCGCCAAGGTC
GCCGACAGCATGGCCAACGTGCTCATTACCGGCGAGAGCGGCACCGGCAAGGAACTCGCCGCCCGCGCCATTCATTACAA
CAGTCCCCGCAAGGGGGCCCCCTTCGTAGCGGTCAACTGTGGCGCCATCCCCGAAACCCTGATCGAGAGCGAGCTCTTCG
GGCACAGCAAGGGGGCCTTCACCGGAGCCTTCGCCGACCGCCCAGGCCTCTTCGAACAGTCCGAGGGGGGGACGCTGTTC
CTGGACGAGATCGGGGAGGTGCCGTTGCAGTTGCAGGCCAAGCTCCTGCGAGTGCTCCAGGAACGGGAATTCCGCCGGGT
CGGCGGGGCGACATCGCTGAAGGCCGACGTGCGCATCGTGGCGGCCTCCAACCGCAACCTCGAGGAGCAGGTGCGGGAGG
GTAGCTTCAGGGAGGACCTCTTCTACCGGCTCAACGTGGTCATGCTGCGCATGCCTTCGCTCAAGGAGCGGGCCGAGGAC
ATCCCTGCCCTGGTCGAGCACTTCTACAAGAAGTACTCCCTTTGGACGGGCGCCGACGAGATCATCACCCCCGACGCGCT
GAAGGCCCTTTTCAACTACCCTTTCCCAGGCAACGTCAGGGAACTCGAGAACCTGGTGGAGCGCTGCGTGGTGCTCGGCA
GCCGCGTCATCGCCCTCGACTGCCTCCCCGCCAGCGTGCGCGAGCACACAGGCGCGCCGCCGGCAAGCGGCGAGACGGAG
ATACCAGACGAGGGGATGGACCTGCAGGTGTACCTGGACAACCTGGAGCAAAAGCTCCTGGTGCAAGCGCTGGACAAGTG
CGGCGGGGTGAAGAAGCGGGCGGCGGCGCTTCTCGGGATGACCTTCCGCTCCTTCCGCTACCGCCTGGCGAAATTCGGCA
TGGACGAAGAATAG

Upstream 100 bases:

>100_bases
TGAGTGGACGTGGGCGGTCAAGGCCGGAATATGAGTGACGGTAAGAAACCACTGGTTGCCGCAGTAAACGGGCGACCCTT
TTTGTCTGAGAGGTGACGCG

Downstream 100 bases:

>100_bases
GTGACGAAAAACGTCACCTGTTTGGTCCCAAAATTGTCGCTGTGCCGCTTTTGGTAAAAATCGCTGTTTTAATATTTCTT
GGCAATAACCCAGTCGCTTC

Product: sigma-54-dependent transcriptional response regulator

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MRARILVVDDELSMREFLSILLEGEGYLVDQAENAEDALRCMGEQRYEMVISDVLMPGLGGIELLSRIKSESPETAVLMI
TAFTTAEQAVEAMKLGAYDYIGKPFKVEEVKVLVRNALEKQSLVQENKRLKAEVQERFSFSGLIGKSKQMREVYDLIAKV
ADSMANVLITGESGTGKELAARAIHYNSPRKGAPFVAVNCGAIPETLIESELFGHSKGAFTGAFADRPGLFEQSEGGTLF
LDEIGEVPLQLQAKLLRVLQEREFRRVGGATSLKADVRIVAASNRNLEEQVREGSFREDLFYRLNVVMLRMPSLKERAED
IPALVEHFYKKYSLWTGADEIITPDALKALFNYPFPGNVRELENLVERCVVLGSRVIALDCLPASVREHTGAPPASGETE
IPDEGMDLQVYLDNLEQKLLVQALDKCGGVKKRAAALLGMTFRSFRYRLAKFGMDEE

Sequences:

>Translated_457_residues
MRARILVVDDELSMREFLSILLEGEGYLVDQAENAEDALRCMGEQRYEMVISDVLMPGLGGIELLSRIKSESPETAVLMI
TAFTTAEQAVEAMKLGAYDYIGKPFKVEEVKVLVRNALEKQSLVQENKRLKAEVQERFSFSGLIGKSKQMREVYDLIAKV
ADSMANVLITGESGTGKELAARAIHYNSPRKGAPFVAVNCGAIPETLIESELFGHSKGAFTGAFADRPGLFEQSEGGTLF
LDEIGEVPLQLQAKLLRVLQEREFRRVGGATSLKADVRIVAASNRNLEEQVREGSFREDLFYRLNVVMLRMPSLKERAED
IPALVEHFYKKYSLWTGADEIITPDALKALFNYPFPGNVRELENLVERCVVLGSRVIALDCLPASVREHTGAPPASGETE
IPDEGMDLQVYLDNLEQKLLVQALDKCGGVKKRAAALLGMTFRSFRYRLAKFGMDEE
>Mature_457_residues
MRARILVVDDELSMREFLSILLEGEGYLVDQAENAEDALRCMGEQRYEMVISDVLMPGLGGIELLSRIKSESPETAVLMI
TAFTTAEQAVEAMKLGAYDYIGKPFKVEEVKVLVRNALEKQSLVQENKRLKAEVQERFSFSGLIGKSKQMREVYDLIAKV
ADSMANVLITGESGTGKELAARAIHYNSPRKGAPFVAVNCGAIPETLIESELFGHSKGAFTGAFADRPGLFEQSEGGTLF
LDEIGEVPLQLQAKLLRVLQEREFRRVGGATSLKADVRIVAASNRNLEEQVREGSFREDLFYRLNVVMLRMPSLKERAED
IPALVEHFYKKYSLWTGADEIITPDALKALFNYPFPGNVRELENLVERCVVLGSRVIALDCLPASVREHTGAPPASGETE
IPDEGMDLQVYLDNLEQKLLVQALDKCGGVKKRAAALLGMTFRSFRYRLAKFGMDEE

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=457, Percent_Identity=44.4201312910284, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1790299, Length=474, Percent_Identity=41.7721518987342, Blast_Score=342, Evalue=3e-95,
Organism=Escherichia coli, GI1790437, Length=450, Percent_Identity=44.2222222222222, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1788905, Length=384, Percent_Identity=41.9270833333333, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI87082117, Length=343, Percent_Identity=43.731778425656, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1789087, Length=407, Percent_Identity=39.8034398034398, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789233, Length=322, Percent_Identity=42.5465838509317, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI87082152, Length=232, Percent_Identity=52.5862068965517, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI1786524, Length=340, Percent_Identity=42.6470588235294, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=41.875, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1787583, Length=331, Percent_Identity=37.4622356495468, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1789828, Length=267, Percent_Identity=34.8314606741573, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87081858, Length=322, Percent_Identity=27.3291925465839, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=31.0924369747899, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1787375, Length=131, Percent_Identity=32.0610687022901, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1786599, Length=124, Percent_Identity=33.0645161290323, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI1786784, Length=121, Percent_Identity=30.5785123966942, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1790863, Length=142, Percent_Identity=31.6901408450704, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87082012, Length=118, Percent_Identity=27.9661016949153, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786911, Length=118, Percent_Identity=31.3559322033898, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788191, Length=105, Percent_Identity=40, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790552, Length=119, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50812; Mature: 50812

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRARILVVDDELSMREFLSILLEGEGYLVDQAENAEDALRCMGEQRYEMVISDVLMPGLG
CCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GIELLSRIKSESPETAVLMITAFTTAEQAVEAMKLGAYDYIGKPFKVEEVKVLVRNALEK
HHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHH
QSLVQENKRLKAEVQERFSFSGLIGKSKQMREVYDLIAKVADSMANVLITGESGTGKELA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHH
ARAIHYNSPRKGAPFVAVNCGAIPETLIESELFGHSKGAFTGAFADRPGLFEQSEGGTLF
HHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCEEE
LDEIGEVPLQLQAKLLRVLQEREFRRVGGATSLKADVRIVAASNRNLEEQVREGSFREDL
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEECCCCHHHHHHCCCHHHHH
FYRLNVVMLRMPSLKERAEDIPALVEHFYKKYSLWTGADEIITPDALKALFNYPFPGNVR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHH
ELENLVERCVVLGSRVIALDCLPASVREHTGAPPASGETEIPDEGMDLQVYLDNLEQKLL
HHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
VQALDKCGGVKKRAAALLGMTFRSFRYRLAKFGMDEE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRARILVVDDELSMREFLSILLEGEGYLVDQAENAEDALRCMGEQRYEMVISDVLMPGLG
CCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
GIELLSRIKSESPETAVLMITAFTTAEQAVEAMKLGAYDYIGKPFKVEEVKVLVRNALEK
HHHHHHHHHCCCCCEEEEEEEEHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHH
QSLVQENKRLKAEVQERFSFSGLIGKSKQMREVYDLIAKVADSMANVLITGESGTGKELA
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHH
ARAIHYNSPRKGAPFVAVNCGAIPETLIESELFGHSKGAFTGAFADRPGLFEQSEGGTLF
HHHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCEEE
LDEIGEVPLQLQAKLLRVLQEREFRRVGGATSLKADVRIVAASNRNLEEQVREGSFREDL
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEECCEEEEEECCCCHHHHHHCCCHHHHH
FYRLNVVMLRMPSLKERAEDIPALVEHFYKKYSLWTGADEIITPDALKALFNYPFPGNVR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCHH
ELENLVERCVVLGSRVIALDCLPASVREHTGAPPASGETEIPDEGMDLQVYLDNLEQKLL
HHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHH
VQALDKCGGVKKRAAALLGMTFRSFRYRLAKFGMDEE
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]