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The map label for this gene is rsbU [H]

Identifier: 197118773

GI number: 197118773

Start: 2773750

End: 2775678

Strand: Direct

Name: rsbU [H]

Synonym: Gbem_2394

Alternate gene names: 197118773

Gene position: 2773750-2775678 (Clockwise)

Preceding gene: 197118772

Following gene: 197118774

Centisome position: 60.1

GC content: 61.85

Gene sequence:

>1929_bases
ATGGGAAAACCGCTTCGCGTGCTGATAGTGGAAGACTCGGAGGACGACGCACTGTTGCTTGTCTTCGAGCTGCGTCGCGG
CAACTATGCTCCGGTGACCAAGCGCGTGGAGAACTCCGACTCGTTGCGCCATGCGCTCAAGGAGGACACCTGGGACCTAG
TCATCTCCGACTACGTCCTTCCCGGATTCTCGGGGCTAGACGCGCTGAAGCTGGTGCGGGCCTCGGGGCTGGACCTTCCC
TTCATCATCGTTTCCGGAAAAATCGGCGAGGAAGATGCCGTAAAGGCCATGAAGGAGGGGGCGAACGATTATCTGATCAA
GGGAAACACCTCGCGCCTTATCCCTGCCATCGAGCGGGAGATGCAGGAGGCCGAGGTTAGGCGCAAGCGGCGCGAGGCCG
AGTCGGCGCTGGTGAGAAGCGAGCGGCGCTACAAGCGGCTGGTGTCGGCGGTCACCGACTATATCTACACCGTAACTATT
CAGAAAGGGGGCGTGGTGAAGACCTCGCATGGCCCCGGATGCCTCTCGGTGACCGGCTACAGCCACGAGGAGTACGTCGA
CAACCCGTTTTTGTGGTACCAGATGATCTACGAGGAGGACCGGGGCGCGGTGACGAGCCTCACCGAGGACCTGCGGGCCG
GAATAGACATCCCCTCGCTGGAGCACCGGATACGGCACAAGGACGGCTCCCTGCGCTGGGTCATCAACACCATAGTCCCA
CGCTACAGCGAACAGGGCGAACTGATCGCCTACGACGGCCTCATCTCGGACATCTCGGAGCGAAAACGCGCCGAAGAGTC
GCTGCAGCTGCAAAGCGCGGCGCTGGAGGCGGCGGCCAACGCCATAGTCATCACCGACAGCAGCGGGGTCATCATCTCGG
TAAACCAGGCCTTCACCAGGATGACCGGGTACAGCCGCGAGGAGGCCCTCGGTCGCGATCTGAGTTTTCTGAAATCGGAG
CGGCACAATTCGGAGTTTTACAGAGGCCTCAGGGAAACCATCAGCGCCGGCGAGGTCTGGCACGGAGAGATGATCAACCG
GCGCAAGGACGGCACCCTCTACCCCGAGGAGCAGACCATCACGCCGGTCCTGGACGAAGACGGGCACATCATCCACTTCA
TCTGCATCAAGCAGGACATCACCGAGCGCAAGCAGGCCGAGCAGGCGCTGATGCAAAACGCCAGCATGCTGAAGGAAATG
GAGATTGCGAAGCAGATCCAGAGTTCTTTGCTTCCGGTCGCCCCCCCCTGCCTCCCGGGCATCGACTGCGCCGGGAGCTG
CTTCCCCGCCAACGACATCGGCGGCGACTACTACGACATCCTCCCGCACGGAGAAGAACTCGACCTCGTCATCGCCGACG
TCTCCGGCCACAGCGTCGGAGCCGCGCTGATCATGGTCGAAACCCGCAGCGTTCTGCGCGCGCAGCTTGCGACCTTGAAG
GGGCCGGCCCAGATCGTGTCGGCCCTGAACGAACTGCTGCACGAGGACCTGAGCCGGGCCGAACTCTTCATCACCATGTC
GTATCTGAGTTACCACATACCCACCGGGACACTCCGCTACACCAACGCCGGCCATCCTCCTCCCCTGCTCTACCGGCATC
AAACCGACCGGTTCTTCGAGCTGGACGCCGAAGGGCTCATCCTGGGGGTACACAGGCAGGTCGCCTTCCAGGAGCCCTCG
CTCCAGGTCCAGGACGGAGACCTGTTGCTGCTGTTCACCGACGGTATCACCGAGGCCGAAAGCCCGGAAGGCGAGTTCTT
CGGCATGGATAGATTGCGGCAGGTAGTGGCGCGCGAGCACAGTAAACCGGCAGCGGGAGTGATCGCCGCAGTCATGGAAT
CAGTCAGGGCCTTCACCGGCTGCGATACCTTCAACGACGACATCTCGATGCTTCTTCTCAAGTTCGGCACCGTAACCCCC
CATTCTTAG

Upstream 100 bases:

>100_bases
TTAGAAACGATGTAAAATGTTTCCAATCTGAGGCAGGGTCCCGTGCCGGGACGCTAGCTGCAGTACCTGCCAAATACCTG
ATCCAGTACTAGGTGCATTC

Downstream 100 bases:

>100_bases
GATGACTTTACTTGACGTCCCCCGGTGCGTTGTGTAAACATACGCCACCTGCACAGGACATGGCCGCCCAGCGCGGCATT
TCCCGTTCCTCCCACTGCGC

Product: response receiver sensor phosphatase

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MGKPLRVLIVEDSEDDALLLVFELRRGNYAPVTKRVENSDSLRHALKEDTWDLVISDYVLPGFSGLDALKLVRASGLDLP
FIIVSGKIGEEDAVKAMKEGANDYLIKGNTSRLIPAIEREMQEAEVRRKRREAESALVRSERRYKRLVSAVTDYIYTVTI
QKGGVVKTSHGPGCLSVTGYSHEEYVDNPFLWYQMIYEEDRGAVTSLTEDLRAGIDIPSLEHRIRHKDGSLRWVINTIVP
RYSEQGELIAYDGLISDISERKRAEESLQLQSAALEAAANAIVITDSSGVIISVNQAFTRMTGYSREEALGRDLSFLKSE
RHNSEFYRGLRETISAGEVWHGEMINRRKDGTLYPEEQTITPVLDEDGHIIHFICIKQDITERKQAEQALMQNASMLKEM
EIAKQIQSSLLPVAPPCLPGIDCAGSCFPANDIGGDYYDILPHGEELDLVIADVSGHSVGAALIMVETRSVLRAQLATLK
GPAQIVSALNELLHEDLSRAELFITMSYLSYHIPTGTLRYTNAGHPPPLLYRHQTDRFFELDAEGLILGVHRQVAFQEPS
LQVQDGDLLLLFTDGITEAESPEGEFFGMDRLRQVVAREHSKPAAGVIAAVMESVRAFTGCDTFNDDISMLLLKFGTVTP
HS

Sequences:

>Translated_642_residues
MGKPLRVLIVEDSEDDALLLVFELRRGNYAPVTKRVENSDSLRHALKEDTWDLVISDYVLPGFSGLDALKLVRASGLDLP
FIIVSGKIGEEDAVKAMKEGANDYLIKGNTSRLIPAIEREMQEAEVRRKRREAESALVRSERRYKRLVSAVTDYIYTVTI
QKGGVVKTSHGPGCLSVTGYSHEEYVDNPFLWYQMIYEEDRGAVTSLTEDLRAGIDIPSLEHRIRHKDGSLRWVINTIVP
RYSEQGELIAYDGLISDISERKRAEESLQLQSAALEAAANAIVITDSSGVIISVNQAFTRMTGYSREEALGRDLSFLKSE
RHNSEFYRGLRETISAGEVWHGEMINRRKDGTLYPEEQTITPVLDEDGHIIHFICIKQDITERKQAEQALMQNASMLKEM
EIAKQIQSSLLPVAPPCLPGIDCAGSCFPANDIGGDYYDILPHGEELDLVIADVSGHSVGAALIMVETRSVLRAQLATLK
GPAQIVSALNELLHEDLSRAELFITMSYLSYHIPTGTLRYTNAGHPPPLLYRHQTDRFFELDAEGLILGVHRQVAFQEPS
LQVQDGDLLLLFTDGITEAESPEGEFFGMDRLRQVVAREHSKPAAGVIAAVMESVRAFTGCDTFNDDISMLLLKFGTVTP
HS
>Mature_641_residues
GKPLRVLIVEDSEDDALLLVFELRRGNYAPVTKRVENSDSLRHALKEDTWDLVISDYVLPGFSGLDALKLVRASGLDLPF
IIVSGKIGEEDAVKAMKEGANDYLIKGNTSRLIPAIEREMQEAEVRRKRREAESALVRSERRYKRLVSAVTDYIYTVTIQ
KGGVVKTSHGPGCLSVTGYSHEEYVDNPFLWYQMIYEEDRGAVTSLTEDLRAGIDIPSLEHRIRHKDGSLRWVINTIVPR
YSEQGELIAYDGLISDISERKRAEESLQLQSAALEAAANAIVITDSSGVIISVNQAFTRMTGYSREEALGRDLSFLKSER
HNSEFYRGLRETISAGEVWHGEMINRRKDGTLYPEEQTITPVLDEDGHIIHFICIKQDITERKQAEQALMQNASMLKEME
IAKQIQSSLLPVAPPCLPGIDCAGSCFPANDIGGDYYDILPHGEELDLVIADVSGHSVGAALIMVETRSVLRAQLATLKG
PAQIVSALNELLHEDLSRAELFITMSYLSYHIPTGTLRYTNAGHPPPLLYRHQTDRFFELDAEGLILGVHRQVAFQEPSL
QVQDGDLLLLFTDGITEAESPEGEFFGMDRLRQVVAREHSKPAAGVIAAVMESVRAFTGCDTFNDDISMLLLKFGTVTPH
S

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

Organism=Homo sapiens, GI71164869, Length=143, Percent_Identity=34.2657342657343, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI71164873, Length=143, Percent_Identity=34.2657342657343, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI26006851, Length=143, Percent_Identity=34.2657342657343, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788394, Length=195, Percent_Identity=27.1794871794872, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1786599, Length=104, Percent_Identity=32.6923076923077, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 71476; Mature: 71345

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPLRVLIVEDSEDDALLLVFELRRGNYAPVTKRVENSDSLRHALKEDTWDLVISDYVL
CCCCEEEEEEECCCCCEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHCCCHHEEEHHHHC
PGFSGLDALKLVRASGLDLPFIIVSGKIGEEDAVKAMKEGANDYLIKGNTSRLIPAIERE
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
MQEAEVRRKRREAESALVRSERRYKRLVSAVTDYIYTVTIQKGGVVKTSHGPGCLSVTGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEECCCCCEEEEECC
SHEEYVDNPFLWYQMIYEEDRGAVTSLTEDLRAGIDIPSLEHRIRHKDGSLRWVINTIVP
CCHHHCCCCCEEHHEEHHHCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEEHHCC
RYSEQGELIAYDGLISDISERKRAEESLQLQSAALEAAANAIVITDSSGVIISVNQAFTR
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEHHHHHH
MTGYSREEALGRDLSFLKSERHNSEFYRGLRETISAGEVWHGEMINRRKDGTLYPEEQTI
HCCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCC
TPVLDEDGHIIHFICIKQDITERKQAEQALMQNASMLKEMEIAKQIQSSLLPVAPPCLPG
CCEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
IDCAGSCFPANDIGGDYYDILPHGEELDLVIADVSGHSVGAALIMVETRSVLRAQLATLK
CCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCEEEEEEEHHHHHHHHHHHCC
GPAQIVSALNELLHEDLSRAELFITMSYLSYHIPTGTLRYTNAGHPPPLLYRHQTDRFFE
CHHHHHHHHHHHHHHHHHHHEEEEEEHHHHEECCCCEEEECCCCCCCCEEEECCCCCEEE
LDAEGLILGVHRQVAFQEPSLQVQDGDLLLLFTDGITEAESPEGEFFGMDRLRQVVAREH
ECCCCEEEEEEHHHEECCCCCEEECCCEEEEEECCCCCCCCCCCCEECHHHHHHHHHHHC
SKPAAGVIAAVMESVRAFTGCDTFNDDISMLLLKFGTVTPHS
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GKPLRVLIVEDSEDDALLLVFELRRGNYAPVTKRVENSDSLRHALKEDTWDLVISDYVL
CCCEEEEEEECCCCCEEEEEEEECCCCCCCHHHHCCCHHHHHHHHHCCCHHEEEHHHHC
PGFSGLDALKLVRASGLDLPFIIVSGKIGEEDAVKAMKEGANDYLIKGNTSRLIPAIERE
CCCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCEEEECCCCCCCHHHHHH
MQEAEVRRKRREAESALVRSERRYKRLVSAVTDYIYTVTIQKGGVVKTSHGPGCLSVTGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEECCCCCEEEEECC
SHEEYVDNPFLWYQMIYEEDRGAVTSLTEDLRAGIDIPSLEHRIRHKDGSLRWVINTIVP
CCHHHCCCCCEEHHEEHHHCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCEEEEEEHHCC
RYSEQGELIAYDGLISDISERKRAEESLQLQSAALEAAANAIVITDSSGVIISVNQAFTR
CCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEHHHHHH
MTGYSREEALGRDLSFLKSERHNSEFYRGLRETISAGEVWHGEMINRRKDGTLYPEEQTI
HCCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCC
TPVLDEDGHIIHFICIKQDITERKQAEQALMQNASMLKEMEIAKQIQSSLLPVAPPCLPG
CCEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
IDCAGSCFPANDIGGDYYDILPHGEELDLVIADVSGHSVGAALIMVETRSVLRAQLATLK
CCCCCCCCCCCCCCCCEEEECCCCCCEEEEEEECCCCCCCEEEEEEEHHHHHHHHHHHCC
GPAQIVSALNELLHEDLSRAELFITMSYLSYHIPTGTLRYTNAGHPPPLLYRHQTDRFFE
CHHHHHHHHHHHHHHHHHHHEEEEEEHHHHEECCCCEEEECCCCCCCCEEEECCCCCEEE
LDAEGLILGVHRQVAFQEPSLQVQDGDLLLLFTDGITEAESPEGEFFGMDRLRQVVAREH
ECCCCEEEEEEHHHEECCCCCEEECCCEEEEEECCCCCCCCCCCCEECHHHHHHHHHHHC
SKPAAGVIAAVMESVRAFTGCDTFNDDISMLLLKFGTVTPHS
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]