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The map label for this gene is pyrE [H]

Identifier: 197118709

GI number: 197118709

Start: 2700224

End: 2700772

Strand: Reverse

Name: pyrE [H]

Synonym: Gbem_2328

Alternate gene names: 197118709

Gene position: 2700772-2700224 (Counterclockwise)

Preceding gene: 197118710

Following gene: 197118708

Centisome position: 58.52

GC content: 60.84

Gene sequence:

>549_bases
ATGAGCGAGAAGGAAAGGCTGAAGAAGATCATCATAGAGCTGTCCTACGAGAAGAGGAACGTCACCCTGGCCTCCGGTCG
CCAGAGCGATTTCTACTTTGACGGCAAGCAGACCACGCTGCACCCGGAAGGTGGCTACCTCACCGGCAAGCTCTTCTTCG
ACGCGATCAAAGACGTCGAAGGGGTGGAAGGGGTAGGGGGGCTCACCCTCGGCGCCGACCCGATCGCGACCGCCACCTCC
ATCGCCAGCTTCCTGGCGGGGAAACCGGTTCCCGGCTTCATCATCAGGAAGGAGCCCAAAGGTCACGGTACCGGCGCCTG
GCTGGAAGGAAGGAAGAACCTGAAGCCCGGCTCCAAGGTCGTCATCGTCGAGGACGTCGTCACCAGCGGCGGTTCCTCCA
TCAAGGCGATCAAGCGCGCCGAAGAGGAAGGGCTTGTGGTTCTGGGTGTAGTCACCCTGGTCGACCGTGAAGAAGGGGGC
CGCGAGAACATCGAGGCCGAAGGGTACTGGCTCAAGTCCATCTTCACCAAGTCCGAGATCCTTGCCTAA

Upstream 100 bases:

>100_bases
GCACTGCCTGCTTCACCGGGGGCTACCCGGTAAAATTCCCGCGGCTTCTCGACCAGGACGAGCCGCAGATGGGGCTATTC
GAAAAGGAGATCGAGAAATA

Downstream 100 bases:

>100_bases
CAAACGGTAGATAGTATTTCACCGAAGGCGGCAATGAAACTATTGCCGCCTTTGTTTTTAGACAAATGCGAACTGCGGAA
CACAGAGGTCCCGGAGGTTC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MSEKERLKKIIIELSYEKRNVTLASGRQSDFYFDGKQTTLHPEGGYLTGKLFFDAIKDVEGVEGVGGLTLGADPIATATS
IASFLAGKPVPGFIIRKEPKGHGTGAWLEGRKNLKPGSKVVIVEDVVTSGGSSIKAIKRAEEEGLVVLGVVTLVDREEGG
RENIEAEGYWLKSIFTKSEILA

Sequences:

>Translated_182_residues
MSEKERLKKIIIELSYEKRNVTLASGRQSDFYFDGKQTTLHPEGGYLTGKLFFDAIKDVEGVEGVGGLTLGADPIATATS
IASFLAGKPVPGFIIRKEPKGHGTGAWLEGRKNLKPGSKVVIVEDVVTSGGSSIKAIKRAEEEGLVVLGVVTLVDREEGG
RENIEAEGYWLKSIFTKSEILA
>Mature_181_residues
SEKERLKKIIIELSYEKRNVTLASGRQSDFYFDGKQTTLHPEGGYLTGKLFFDAIKDVEGVEGVGGLTLGADPIATATSI
ASFLAGKPVPGFIIRKEPKGHGTGAWLEGRKNLKPGSKVVIVEDVVTSGGSSIKAIKRAEEEGLVVLGVVTLVDREEGGR
ENIEAEGYWLKSIFTKSEILA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=163, Percent_Identity=32.5153374233129, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17554964, Length=164, Percent_Identity=35.3658536585366, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17933654, Length=157, Percent_Identity=36.3057324840764, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 19609; Mature: 19477

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKERLKKIIIELSYEKRNVTLASGRQSDFYFDGKQTTLHPEGGYLTGKLFFDAIKDVE
CCHHHHHHHHHHHEECCCCEEEEECCCCCCEEECCCCEEECCCCCEEEHHHHHHHHHHCC
GVEGVGGLTLGADPIATATSIASFLAGKPVPGFIIRKEPKGHGTGAWLEGRKNLKPGSKV
CCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEECCCCCCCCCCEE
VIVEDVVTSGGSSIKAIKRAEEEGLVVLGVVTLVDREEGGRENIEAEGYWLKSIFTKSEI
EEEEEHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHH
LA
CC
>Mature Secondary Structure 
SEKERLKKIIIELSYEKRNVTLASGRQSDFYFDGKQTTLHPEGGYLTGKLFFDAIKDVE
CHHHHHHHHHHHEECCCCEEEEECCCCCCEEECCCCEEECCCCCEEEHHHHHHHHHHCC
GVEGVGGLTLGADPIATATSIASFLAGKPVPGFIIRKEPKGHGTGAWLEGRKNLKPGSKV
CCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEECCCCCCCCCCEE
VIVEDVVTSGGSSIKAIKRAEEEGLVVLGVVTLVDREEGGRENIEAEGYWLKSIFTKSEI
EEEEEHHHCCCCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHH
LA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA