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The map label for this gene is purF [H]

Identifier: 197118710

GI number: 197118710

Start: 2700772

End: 2702205

Strand: Reverse

Name: purF [H]

Synonym: Gbem_2329

Alternate gene names: 197118710

Gene position: 2702205-2700772 (Counterclockwise)

Preceding gene: 197118711

Following gene: 197118709

Centisome position: 58.55

GC content: 60.74

Gene sequence:

>1434_bases
GTGGAAGAAATGATGATGCGCAGGCCCGAAGAAGAGTGCGGCATTTTCGGTGTATTCAACCACCCGGAGGCCTCGAACCT
CACCTACCTCGGACTCTACGCCCTTCAGCACAGGGGACAGGAAAGTTGCGGTATCGTCTCCTCCGACGGAAACAACCTGC
ACTCCCACAAGAGCATGGGACTTGTCGCCGACGTTTTCGGCAATCAGGAAATCTTCAAATCGCTGCCGGGCAAGGCTGCC
ATAGGCCACGTCAGGTACTCCACCACCGGCTCGTCGGTGATCAAGAACGTGCAGCCGATCATGGTGGACTACTCCCGCGG
CTCCATCGCCGTGGCCCATAACGGCAACATCGTCAACGCCCAGATCATCAAGGATGAGCTCGAAGCGTACGGATCGATCT
TCCAGACGACCATGGACACCGAGATCATCGTGCACCTTCTGGCAACCTCGAAGGCCATCTCGCTGCAGGACCGGCTCACC
GACGCGCTCAGCCGCATCCAGGGGGCCTACTGCCTGCTCTTCCTGACCGAAACCCGCATGGTCGCCGTCCGCGACCCCAA
CGGTTTCCGTCCGCTTTGCCTCGGGCGCCTGGGGGGCTCCTATGTGGTGGCTTCCGAGAGCTGCGCCCTCGACCTCATCG
ATGCCGAATTCATCCGCGAGATCGCGCCGGGCGAGATGATAGTCGTCGATAAAAACGGCATGTCCTCCTTCTTCCCGTTC
AAGAAGGTCGATCCCACCCCCTGCATCTTCGAATTCGTCTATTTCGCCCGTCCCGACTCCCACATCTTCGGGAAAAACGT
GTACCAAGTGCGCAAGGAGCAGGGGCGCCAGTTGGCCCGCGAACACAGGGTGGACGCCGACATCGTCATCCCGATCCCCG
ATTCCGGGGTTCCCGCGGCGCTCGGCTACGCCGAGGAGTCCGGCATCCCGTTCGAGCTGGGGCTGATCCGCAACCACTAC
GTGGGGCGTACCTTCATCGAGCCGCAGCAGGCCATCCGCCACTTCGGCGTCAAGATCAAGCTGAACCCGGTGCGCGAGGT
GCTGAAGGACAAGCGCGTGGTCGTCATCGACGACTCCATCGTGCGCGGCACCACCTCCAGGAAAATCGTCAAGATGATCC
GCAACGCAGGCGCGAGGGAAGTGCACGTGCGCATCTCTTCGCCTCCGACCAGCTACCCCTGCTACTACGGGATCGATACC
CCCAACCGTAAAGAGCTGATCTCCTCGTCGCACTCGCTCGACGAGATCCGCCGCTACATAACGGCCGACTCGCTCGGCTA
TCTTTCCGAAGAAGGTCTCATGTCATCCGTCGGCGCGGAAAATGCCGGCTTCTGCACTGCCTGCTTCACCGGGGGCTACC
CGGTAAAATTCCCGCGGCTTCTCGACCAGGACGAGCCGCAGATGGGGCTATTCGAAAAGGAGATCGAGAAATAA

Upstream 100 bases:

>100_bases
GATGGACTGATCTCCCCACCGAGGGGAAGAATGGTGGACGATCCCTTCGGGGATACAAAGAAGGATCGACAGGTAAGCAG
TTCTTTTATAAGGAGCAGTC

Downstream 100 bases:

>100_bases
TGAGCGAGAAGGAAAGGCTGAAGAAGATCATCATAGAGCTGTCCTACGAGAAGAGGAACGTCACCCTGGCCTCCGGTCGC
CAGAGCGATTTCTACTTTGA

Product: glutamine--phosphoribosylpyrophosphate amidotransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MEEMMMRRPEEECGIFGVFNHPEASNLTYLGLYALQHRGQESCGIVSSDGNNLHSHKSMGLVADVFGNQEIFKSLPGKAA
IGHVRYSTTGSSVIKNVQPIMVDYSRGSIAVAHNGNIVNAQIIKDELEAYGSIFQTTMDTEIIVHLLATSKAISLQDRLT
DALSRIQGAYCLLFLTETRMVAVRDPNGFRPLCLGRLGGSYVVASESCALDLIDAEFIREIAPGEMIVVDKNGMSSFFPF
KKVDPTPCIFEFVYFARPDSHIFGKNVYQVRKEQGRQLAREHRVDADIVIPIPDSGVPAALGYAEESGIPFELGLIRNHY
VGRTFIEPQQAIRHFGVKIKLNPVREVLKDKRVVVIDDSIVRGTTSRKIVKMIRNAGAREVHVRISSPPTSYPCYYGIDT
PNRKELISSSHSLDEIRRYITADSLGYLSEEGLMSSVGAENAGFCTACFTGGYPVKFPRLLDQDEPQMGLFEKEIEK

Sequences:

>Translated_477_residues
MEEMMMRRPEEECGIFGVFNHPEASNLTYLGLYALQHRGQESCGIVSSDGNNLHSHKSMGLVADVFGNQEIFKSLPGKAA
IGHVRYSTTGSSVIKNVQPIMVDYSRGSIAVAHNGNIVNAQIIKDELEAYGSIFQTTMDTEIIVHLLATSKAISLQDRLT
DALSRIQGAYCLLFLTETRMVAVRDPNGFRPLCLGRLGGSYVVASESCALDLIDAEFIREIAPGEMIVVDKNGMSSFFPF
KKVDPTPCIFEFVYFARPDSHIFGKNVYQVRKEQGRQLAREHRVDADIVIPIPDSGVPAALGYAEESGIPFELGLIRNHY
VGRTFIEPQQAIRHFGVKIKLNPVREVLKDKRVVVIDDSIVRGTTSRKIVKMIRNAGAREVHVRISSPPTSYPCYYGIDT
PNRKELISSSHSLDEIRRYITADSLGYLSEEGLMSSVGAENAGFCTACFTGGYPVKFPRLLDQDEPQMGLFEKEIEK
>Mature_477_residues
MEEMMMRRPEEECGIFGVFNHPEASNLTYLGLYALQHRGQESCGIVSSDGNNLHSHKSMGLVADVFGNQEIFKSLPGKAA
IGHVRYSTTGSSVIKNVQPIMVDYSRGSIAVAHNGNIVNAQIIKDELEAYGSIFQTTMDTEIIVHLLATSKAISLQDRLT
DALSRIQGAYCLLFLTETRMVAVRDPNGFRPLCLGRLGGSYVVASESCALDLIDAEFIREIAPGEMIVVDKNGMSSFFPF
KKVDPTPCIFEFVYFARPDSHIFGKNVYQVRKEQGRQLAREHRVDADIVIPIPDSGVPAALGYAEESGIPFELGLIRNHY
VGRTFIEPQQAIRHFGVKIKLNPVREVLKDKRVVVIDDSIVRGTTSRKIVKMIRNAGAREVHVRISSPPTSYPCYYGIDT
PNRKELISSSHSLDEIRRYITADSLGYLSEEGLMSSVGAENAGFCTACFTGGYPVKFPRLLDQDEPQMGLFEKEIEK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=517, Percent_Identity=40.8123791102514, Blast_Score=373, Evalue=1e-103,
Organism=Escherichia coli, GI1788651, Length=478, Percent_Identity=40.7949790794979, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1790167, Length=231, Percent_Identity=26.4069264069264, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=476, Percent_Identity=42.2268907563025, Blast_Score=352, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6323958, Length=472, Percent_Identity=35.1694915254237, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=42.0408163265306, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI28573187, Length=490, Percent_Identity=42.4489795918367, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=43.1330472103004, Blast_Score=367, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52938; Mature: 52938

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEMMMRRPEEECGIFGVFNHPEASNLTYLGLYALQHRGQESCGIVSSDGNNLHSHKSMG
CCCHHHCCCHHHCCEEEEECCCCCCCEEEEEHHHHHHCCCCCCCEEECCCCCCCCCCCCC
LVADVFGNQEIFKSLPGKAAIGHVRYSTTGSSVIKNVQPIMVDYSRGSIAVAHNGNIVNA
CHHHHHCCHHHHHHCCCCEEEEEEEEECCHHHHHHCCCEEEEEECCCCEEEEECCCEEEH
QIIKDELEAYGSIFQTTMDTEIIVHLLATSKAISLQDRLTDALSRIQGAYCLLFLTETRM
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCEE
VAVRDPNGFRPLCLGRLGGSYVVASESCALDLIDAEFIREIAPGEMIVVDKNGMSSFFPF
EEEECCCCCCCHHHHCCCCCEEEECCCCEEEHHHHHHHHHCCCCCEEEEECCCCCCCCCC
KKVDPTPCIFEFVYFARPDSHIFGKNVYQVRKEQGRQLAREHRVDADIVIPIPDSGVPAA
CCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCE
LGYAEESGIPFELGLIRNHYVGRTFIEPQQAIRHFGVKIKLNPVREVLKDKRVVVIDDSI
ECCHHCCCCCEEEEEHHCCCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHCCCEEEECCCH
VRGTTSRKIVKMIRNAGAREVHVRISSPPTSYPCYYGIDTPNRKELISSSHSLDEIRRYI
HCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHH
TADSLGYLSEEGLMSSVGAENAGFCTACFTGGYPVKFPRLLDQDEPQMGLFEKEIEK
HHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCEECCHHCCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MEEMMMRRPEEECGIFGVFNHPEASNLTYLGLYALQHRGQESCGIVSSDGNNLHSHKSMG
CCCHHHCCCHHHCCEEEEECCCCCCCEEEEEHHHHHHCCCCCCCEEECCCCCCCCCCCCC
LVADVFGNQEIFKSLPGKAAIGHVRYSTTGSSVIKNVQPIMVDYSRGSIAVAHNGNIVNA
CHHHHHCCHHHHHHCCCCEEEEEEEEECCHHHHHHCCCEEEEEECCCCEEEEECCCEEEH
QIIKDELEAYGSIFQTTMDTEIIVHLLATSKAISLQDRLTDALSRIQGAYCLLFLTETRM
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEEEEEECCEE
VAVRDPNGFRPLCLGRLGGSYVVASESCALDLIDAEFIREIAPGEMIVVDKNGMSSFFPF
EEEECCCCCCCHHHHCCCCCEEEECCCCEEEHHHHHHHHHCCCCCEEEEECCCCCCCCCC
KKVDPTPCIFEFVYFARPDSHIFGKNVYQVRKEQGRQLAREHRVDADIVIPIPDSGVPAA
CCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCE
LGYAEESGIPFELGLIRNHYVGRTFIEPQQAIRHFGVKIKLNPVREVLKDKRVVVIDDSI
ECCHHCCCCCEEEEEHHCCCCCCCCCCHHHHHHHCCCEEEEHHHHHHHHCCCEEEECCCH
VRGTTSRKIVKMIRNAGAREVHVRISSPPTSYPCYYGIDTPNRKELISSSHSLDEIRRYI
HCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCHHHHHHCCCCHHHHHHHH
TADSLGYLSEEGLMSSVGAENAGFCTACFTGGYPVKFPRLLDQDEPQMGLFEKEIEK
HHHHCCCCCCCCHHHHCCCCCCCEEEEEECCCCCEECCHHCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]