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The map label for this gene is zraS [H]

Identifier: 197118651

GI number: 197118651

Start: 2640421

End: 2642406

Strand: Direct

Name: zraS [H]

Synonym: Gbem_2270

Alternate gene names: 197118651

Gene position: 2640421-2642406 (Clockwise)

Preceding gene: 197118650

Following gene: 197118654

Centisome position: 57.21

GC content: 64.8

Gene sequence:

>1986_bases
ATGACTCTTCCCAAGCTGCGCTTTCCCATAAAGACCAAGCTCACCGTGGCGACGCTCATCCCGCTGGGGATAGCGATCCT
CATCTGCTGGATGGCCGGGGTCTTCATTTTGAGCTCCAAGGTCGCCGCCCAGGCCCAGGAGAAGGTCCGCTACGACCTGA
GTGTCGCCCGCGAGGCTTACCAGAGCGAGCTTTCGCGCATCTACGACGTGGTGAAGCTCACCGCCTCCTTCGGCAGGACC
GCCGAGACCATCGCCTCCGGCGACCGGCGGGCGGTGAGCGAGTCGCTTTCTTCATTGCGCGCCAGCGAGCGCCTGGACAT
CCTCGCCGCCGTAGACGCCTCGGGAAAGGTGATCTTCAGGGCCAACAACCCCGAGCTGCGCGGCGACGACAAGACCCGCA
ACCAGTTCGTAGCGCGCGCTTTGAAGGGGGAGATCGCCAGCGGCACCACCATCCTCCCCTCTTCCGAGCTTGAGCTCGAA
GGAGACAGCCTGGTGCGCCAGGCGCGCATCTTCCCCGCCGCAGGAACCCCGGTGCAGCTAAACGGCGCCATGTTCCTCCT
GGTCGCGGCACCGGTGCGGGACAAGGCGGGGAACCTGCTGGGGGCGCTCTACGGCGGGGTGCTCCTCAACAACAACCAGA
AGCTCGTGGACCGGATCCGCTCCGTGGTCTACGAGGGGGCGCGGAAAAACGGCCGGGACGTTGGTAACTCCACCATCTTC
CAGGGGGACATAAGGATCGCCACCAACGTCCCCAACACCGACGGCAGCCGGGCCGTCGGCACCAGGCTCTCCGCGCCGGT
CCAGGAGCGGGTGCTCCTCAAGGGGGCCAAGTGGGTCGGGCGCGCCTTCGTGGTGAACGACTGGTACCTGACCGCCTACG
AACCGATCCTCTCGCTGCAGGGGGTCCCGATCGGCGCCCTCTACGTGGGGATGCTGGAGAGCCAGTATTCGGCGGTCAAG
ATCGACATGGCCGTCCTTTTGAGCTTCGTCCTTTTGGTGAGCGGCCTGGTGGGGGTCTCCATGGCCGGGTTCCTGGGGAG
GAAGCTCTCCCAGCCCATACGGGAGTTGGACCTTCTGGCGCGACGCGTAGCCGCAGGCGAGCGCAACGTGAAAAGCAGCA
TCGACTCCCGGGACGAGATCGGCGACCTGGCGGTGCGGTTCAACGACATGAGCCGTTCGCTCGTGGAGCGCGAGGACTCG
ATCATCGAACTGAACCGCAACCTGGAAGAGAAGGTGCAGTCGAGAACGGCGGAACTGGAGGAGAAGAACCGGCTCCTGGT
GCAGACCCGCGAGGAGTTGTTGCGGGTGGAGAAGCTGGCGGCGATAGGCGAGCTGGCTGCGGGGGTCGCGCACGAGATCA
ACAACCCGATGGCGATCATCCGCGGCAACACGGAGTTGTTGCAGCTCTCGGTGCCGGAGGAAGCCCCGATCCGTGAGGAA
GTGGACACCATTTTCCAGCAGGTGAAAAGGGTGGAGCGGATCGTATCGAACCTGTTGAAATTCGCCAGGCGCGAGCAGAT
GGAAGCTGGAGCCGTGCGGCTGAACGAGCTCTTGCACGAGATCGTGGGCCAGATCGGGCACCAGGTGTCGCTTGAGAGGA
TAGAGATAGCGGAGCAGTATGCGGAGAGCGTGGCGCAGGTGGAGGGGGATGCGGACCAGTTGCGTCAGGTGTTCACCAAC
CTGGTCTTGAACGCGGTGCAGGCGATGCCGTCAGGGGGCGTTCTCTCGGTGAGGACCCAGCAGGTGGAGCCCGCGGGGAG
CTACGAGGTAAAAGTCGGCGACACCGGGGTCGGCATCGCGCTGGAGAACCTGAGACAGGTATTCAACCCCTTCTACACCA
CCAAGGCCAACGGCACCGGCCTCGGACTTTCCGTATCCTACGGCATCGTCCGGGAGCATGGAGGGCGCATCGACGTCGAG
AGCGTCCCGGGCGGCGGGAGCACCTTCACCGTGGTGCTCCCGCGCTCCCAAACGCCGGGGGCCTAG

Upstream 100 bases:

>100_bases
CCGGAACCGGGTACCACAAGAGCAGGTCGGCCGAGATCCTGGGCATCTCCAGAAAAACCCTGGACCGCAAGATCGTCGAA
TACCGCATCACCATTCCCTC

Downstream 100 bases:

>100_bases
AGTATCGCCACCGCATCACCGGGGCGCACCACGCCCCCCTTCAGCACCTTGGCGAAGATCCCTTCCTTGGGCATGACGCA
GTCGCCGGCCTGGTAGTAGA

Product: HAMP domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 661; Mature: 660

Protein sequence:

>661_residues
MTLPKLRFPIKTKLTVATLIPLGIAILICWMAGVFILSSKVAAQAQEKVRYDLSVAREAYQSELSRIYDVVKLTASFGRT
AETIASGDRRAVSESLSSLRASERLDILAAVDASGKVIFRANNPELRGDDKTRNQFVARALKGEIASGTTILPSSELELE
GDSLVRQARIFPAAGTPVQLNGAMFLLVAAPVRDKAGNLLGALYGGVLLNNNQKLVDRIRSVVYEGARKNGRDVGNSTIF
QGDIRIATNVPNTDGSRAVGTRLSAPVQERVLLKGAKWVGRAFVVNDWYLTAYEPILSLQGVPIGALYVGMLESQYSAVK
IDMAVLLSFVLLVSGLVGVSMAGFLGRKLSQPIRELDLLARRVAAGERNVKSSIDSRDEIGDLAVRFNDMSRSLVEREDS
IIELNRNLEEKVQSRTAELEEKNRLLVQTREELLRVEKLAAIGELAAGVAHEINNPMAIIRGNTELLQLSVPEEAPIREE
VDTIFQQVKRVERIVSNLLKFARREQMEAGAVRLNELLHEIVGQIGHQVSLERIEIAEQYAESVAQVEGDADQLRQVFTN
LVLNAVQAMPSGGVLSVRTQQVEPAGSYEVKVGDTGVGIALENLRQVFNPFYTTKANGTGLGLSVSYGIVREHGGRIDVE
SVPGGGSTFTVVLPRSQTPGA

Sequences:

>Translated_661_residues
MTLPKLRFPIKTKLTVATLIPLGIAILICWMAGVFILSSKVAAQAQEKVRYDLSVAREAYQSELSRIYDVVKLTASFGRT
AETIASGDRRAVSESLSSLRASERLDILAAVDASGKVIFRANNPELRGDDKTRNQFVARALKGEIASGTTILPSSELELE
GDSLVRQARIFPAAGTPVQLNGAMFLLVAAPVRDKAGNLLGALYGGVLLNNNQKLVDRIRSVVYEGARKNGRDVGNSTIF
QGDIRIATNVPNTDGSRAVGTRLSAPVQERVLLKGAKWVGRAFVVNDWYLTAYEPILSLQGVPIGALYVGMLESQYSAVK
IDMAVLLSFVLLVSGLVGVSMAGFLGRKLSQPIRELDLLARRVAAGERNVKSSIDSRDEIGDLAVRFNDMSRSLVEREDS
IIELNRNLEEKVQSRTAELEEKNRLLVQTREELLRVEKLAAIGELAAGVAHEINNPMAIIRGNTELLQLSVPEEAPIREE
VDTIFQQVKRVERIVSNLLKFARREQMEAGAVRLNELLHEIVGQIGHQVSLERIEIAEQYAESVAQVEGDADQLRQVFTN
LVLNAVQAMPSGGVLSVRTQQVEPAGSYEVKVGDTGVGIALENLRQVFNPFYTTKANGTGLGLSVSYGIVREHGGRIDVE
SVPGGGSTFTVVLPRSQTPGA
>Mature_660_residues
TLPKLRFPIKTKLTVATLIPLGIAILICWMAGVFILSSKVAAQAQEKVRYDLSVAREAYQSELSRIYDVVKLTASFGRTA
ETIASGDRRAVSESLSSLRASERLDILAAVDASGKVIFRANNPELRGDDKTRNQFVARALKGEIASGTTILPSSELELEG
DSLVRQARIFPAAGTPVQLNGAMFLLVAAPVRDKAGNLLGALYGGVLLNNNQKLVDRIRSVVYEGARKNGRDVGNSTIFQ
GDIRIATNVPNTDGSRAVGTRLSAPVQERVLLKGAKWVGRAFVVNDWYLTAYEPILSLQGVPIGALYVGMLESQYSAVKI
DMAVLLSFVLLVSGLVGVSMAGFLGRKLSQPIRELDLLARRVAAGERNVKSSIDSRDEIGDLAVRFNDMSRSLVEREDSI
IELNRNLEEKVQSRTAELEEKNRLLVQTREELLRVEKLAAIGELAAGVAHEINNPMAIIRGNTELLQLSVPEEAPIREEV
DTIFQQVKRVERIVSNLLKFARREQMEAGAVRLNELLHEIVGQIGHQVSLERIEIAEQYAESVAQVEGDADQLRQVFTNL
VLNAVQAMPSGGVLSVRTQQVEPAGSYEVKVGDTGVGIALENLRQVFNPFYTTKANGTGLGLSVSYGIVREHGGRIDVES
VPGGGSTFTVVLPRSQTPGA

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=233, Percent_Identity=38.1974248927039, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1788549, Length=228, Percent_Identity=34.2105263157895, Blast_Score=150, Evalue=3e-37,
Organism=Escherichia coli, GI1790300, Length=250, Percent_Identity=30.8, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1786600, Length=287, Percent_Identity=29.9651567944251, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788393, Length=318, Percent_Identity=26.1006289308176, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI87081816, Length=367, Percent_Identity=28.0653950953678, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1789149, Length=355, Percent_Identity=26.7605633802817, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI145693157, Length=223, Percent_Identity=30.4932735426009, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=252, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1790861, Length=243, Percent_Identity=28.3950617283951, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=261, Percent_Identity=25.6704980842912, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1790346, Length=219, Percent_Identity=22.8310502283105, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI48994928, Length=115, Percent_Identity=38.2608695652174, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788713, Length=253, Percent_Identity=24.5059288537549, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 71856; Mature: 71724

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPKLRFPIKTKLTVATLIPLGIAILICWMAGVFILSSKVAAQAQEKVRYDLSVAREAY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSELSRIYDVVKLTASFGRTAETIASGDRRAVSESLSSLRASERLDILAAVDASGKVIFR
HHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEE
ANNPELRGDDKTRNQFVARALKGEIASGTTILPSSELELEGDSLVRQARIFPAAGTPVQL
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCCCEEE
NGAMFLLVAAPVRDKAGNLLGALYGGVLLNNNQKLVDRIRSVVYEGARKNGRDVGNSTIF
CCEEEEEEECCCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
QGDIRIATNVPNTDGSRAVGTRLSAPVQERVLLKGAKWVGRAFVVNDWYLTAYEPILSLQ
ECCEEEEECCCCCCCCCHHCCCCCCCHHHHHHHHCCHHHCCEEEEECEEHHHHHHHHHCC
GVPIGALYVGMLESQYSAVKIDMAVLLSFVLLVSGLVGVSMAGFLGRKLSQPIRELDLLA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRVAAGERNVKSSIDSRDEIGDLAVRFNDMSRSLVEREDSIIELNRNLEEKVQSRTAELE
HHHHCCCCHHHHHCCCHHHHHHHHEEEHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHH
EKNRLLVQTREELLRVEKLAAIGELAAGVAHEINNPMAIIRGNTELLQLSVPEEAPIREE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCHHH
VDTIFQQVKRVERIVSNLLKFARREQMEAGAVRLNELLHEIVGQIGHQVSLERIEIAEQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AESVAQVEGDADQLRQVFTNLVLNAVQAMPSGGVLSVRTQQVEPAGSYEVKVGDTGVGIA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEECCCCCHHH
LENLRQVFNPFYTTKANGTGLGLSVSYGIVREHGGRIDVESVPGGGSTFTVVLPRSQTPG
HHHHHHHHCCCEECCCCCCCCEEEEEHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCC
A
C
>Mature Secondary Structure 
TLPKLRFPIKTKLTVATLIPLGIAILICWMAGVFILSSKVAAQAQEKVRYDLSVAREAY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSELSRIYDVVKLTASFGRTAETIASGDRRAVSESLSSLRASERLDILAAVDASGKVIFR
HHHHHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEE
ANNPELRGDDKTRNQFVARALKGEIASGTTILPSSELELEGDSLVRQARIFPAAGTPVQL
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCCCEEE
NGAMFLLVAAPVRDKAGNLLGALYGGVLLNNNQKLVDRIRSVVYEGARKNGRDVGNSTIF
CCEEEEEEECCCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEE
QGDIRIATNVPNTDGSRAVGTRLSAPVQERVLLKGAKWVGRAFVVNDWYLTAYEPILSLQ
ECCEEEEECCCCCCCCCHHCCCCCCCHHHHHHHHCCHHHCCEEEEECEEHHHHHHHHHCC
GVPIGALYVGMLESQYSAVKIDMAVLLSFVLLVSGLVGVSMAGFLGRKLSQPIRELDLLA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RRVAAGERNVKSSIDSRDEIGDLAVRFNDMSRSLVEREDSIIELNRNLEEKVQSRTAELE
HHHHCCCCHHHHHCCCHHHHHHHHEEEHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHH
EKNRLLVQTREELLRVEKLAAIGELAAGVAHEINNPMAIIRGNTELLQLSVPEEAPIREE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEECCCCCCCHHH
VDTIFQQVKRVERIVSNLLKFARREQMEAGAVRLNELLHEIVGQIGHQVSLERIEIAEQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
AESVAQVEGDADQLRQVFTNLVLNAVQAMPSGGVLSVRTQQVEPAGSYEVKVGDTGVGIA
HHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEECCCCCHHH
LENLRQVFNPFYTTKANGTGLGLSVSYGIVREHGGRIDVESVPGGGSTFTVVLPRSQTPG
HHHHHHHHCCCEECCCCCCCCEEEEEHHHHHHCCCEEEEEECCCCCCEEEEEEECCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]