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The map label for this gene is zraS [H]

Identifier: 197118621

GI number: 197118621

Start: 2609918

End: 2611936

Strand: Direct

Name: zraS [H]

Synonym: Gbem_2240

Alternate gene names: 197118621

Gene position: 2609918-2611936 (Clockwise)

Preceding gene: 197118620

Following gene: 197118622

Centisome position: 56.55

GC content: 62.31

Gene sequence:

>2019_bases
ATGCTCAAGCGTTTCCCGATCAAGGCCAAGCTCACCTTCGGGGCGCTGGCGCCGCTTTTCGTCGCCTTCTTCATCTGCTC
CCTGGCCGGCCTCTACATCATCAACGAGAAGATCGCGAGCCAGGCGCAGGAAAAGGTGCGCACCGACCTCAACTCCGCGC
GTGAGGTCTACAGAAACGAGCTGGACCGGATCCGCGAGTTCATCGATCTCACCGCCACCAACCCCTACAACTCCTCCTCC
ATCGTCGCCGGCGATCATGAAATCCAGGCTCTTTTGCGCCAGCGGCTCTACAAGAAACGGCTGGACATCCTGACCGCCGT
GGACGCGAAGGGGCGGGTTTTGTACCGTGCCCACACCCCGAGCCTTGCCGGCGACCTGCAAAAGAGCTACTTCGTGCAGC
AGGCGCTGAAAGGGGTGGCGGTAACGGGGACGGCGCTTATGGGCGAGCAGGAACTGGCCCGGGAGGGTGTGGCGCTATCC
AGCCGCGCCACCATTCCGCTGGTCTCCACGCCGCATGCCCGTCCGCGCAACGACGCGATCGAAAAAACGGGGATGGTAAT
GGTCTCCGCCGCCCCCTTGAGAAACCATGCCGGGCAGATCATAGGCGCCCTGTACGGCGCGGTCCTGCTCAACAACAACA
ACGCCCTGGTCGACAAGATCAAAGAGATCGTGTACGAGGGGGTGCAGTTCAACGGCACGGACGTGGGAAGCGCCACCATC
TTTTTGGGCGACGCCCGGATCGCGACCAACGTCCGCCTGACCGACGGCGCCCGCGCCATAGGGACCAGGGTATCCGAAGA
GGTGTACCAGCGGGTGATCGTGGAGAAAAAGAAGTGGATCCGGCGCGCCTTCGTGGTGAACGACTGGTACTTCACCGCCT
ACGAGCCGATCCTGGACCTGCATGGAAAGGCGATCGGGTCGCTCTACGTCGGGATGCTGGAGAAGCCTTACACCCACATG
CAAAAAAGCGTCAACTCGATCCTGTACATGGTGCTCTTCGTCACCTCGCTGATCGGGCTTGCGGTCTCCGGCTTCATCGC
CACCCTCCTCGCCCGTCCCATCAAGGAGCTGGAGAAGCTGGCGCACCGGGTGGCGCGCGGTGAGCGCAACCTGCAGATGG
AGGTGCACACAAAGGACGAGGTCGGGGACCTGGCCGACGCCTTCAACCTGATGACCAGAGCCCTGAGCCGCCAGGAGGCC
GAAATAGGTCTCTTGCACCGCGCGCTGGAGCTTAAGGTGGAGGAGCGGACCGCGCAACTCTCCGACAAGAACCGCCTGCT
TTTGCAGACCCAGGCGGACCTGGCCCGCGCCGAGAAGCTTGCCGACCTGGGGATCGTCGCCGCCGGCGTAGCCCACGAGA
TCAACACCCCGCTTGCCATCATCCGCGGCAACGCCGAGGTGCTGGAGATGTGCCTTCCGCCCGAGCATCCCAACCATGAG
GAGGTCGAGATCATCAGCATGCAGACCGAGCGCATGGCGAAGATCGTCGGCAACCTGCTCACCTTCGCGCGCCAGAAATC
CCTGAACCAGAGGGAGTTCATGGTGCACGAGATCCTGGACGACATCGTGACGCAGATCAGGCATCAGGTTCCCATGGACG
CCATCTCGGTGCAGTGGGAATACGACATGAACCTGGGTGCGGTTACTGGGGATACCGACCAGCTGCGGCAGGTATTCAGC
AACATCATCCTGAACGCGGTGCAGGCGATGCTTCCCGAGGGAGGAACCCTCAGGCTCACCACCCGGCCCCACGGCCCCGG
CAACGGATGCGAAGTTGAGATCCGCGACACCGGCAAAGGTATCCCTGCCGAGCATCTGGAAAAGATCTTCACCCCGTTTT
TCACCACCAGGGACAGCGGCACCGGGCTTGGCCTCTCCGTTTCCTACGGCATCGTTAGGGACCACGGCGGCGACATCCAG
GTTTCCAGCACGCCGGGAGCCGGTACCTGCTTCAAGATTGTCTTGCCGGGGGAAACGGAAACGGCACAGGAAACGCCGGA
AAACGTCGCAGATCTTTAA

Upstream 100 bases:

>100_bases
GGAACAACAAAAGCCGCGCCGCGAAGATCCTCGAGATAGCCAGGCGCACGCTGGACCGGAAGATCGAGGAGTACGGCCTG
GAAGACGGGGGCTCCCGCTG

Downstream 100 bases:

>100_bases
AGTAAAACTTTCATTTCAGAGGAGCAAATACATGAGTATGCAGTGGACCAGCGATCTAGCGACAGGAGTAGCGGAAATAG
ATAACCAGCACAAGGAGATT

Product: HAMP domain-containing sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MLKRFPIKAKLTFGALAPLFVAFFICSLAGLYIINEKIASQAQEKVRTDLNSAREVYRNELDRIREFIDLTATNPYNSSS
IVAGDHEIQALLRQRLYKKRLDILTAVDAKGRVLYRAHTPSLAGDLQKSYFVQQALKGVAVTGTALMGEQELAREGVALS
SRATIPLVSTPHARPRNDAIEKTGMVMVSAAPLRNHAGQIIGALYGAVLLNNNNALVDKIKEIVYEGVQFNGTDVGSATI
FLGDARIATNVRLTDGARAIGTRVSEEVYQRVIVEKKKWIRRAFVVNDWYFTAYEPILDLHGKAIGSLYVGMLEKPYTHM
QKSVNSILYMVLFVTSLIGLAVSGFIATLLARPIKELEKLAHRVARGERNLQMEVHTKDEVGDLADAFNLMTRALSRQEA
EIGLLHRALELKVEERTAQLSDKNRLLLQTQADLARAEKLADLGIVAAGVAHEINTPLAIIRGNAEVLEMCLPPEHPNHE
EVEIISMQTERMAKIVGNLLTFARQKSLNQREFMVHEILDDIVTQIRHQVPMDAISVQWEYDMNLGAVTGDTDQLRQVFS
NIILNAVQAMLPEGGTLRLTTRPHGPGNGCEVEIRDTGKGIPAEHLEKIFTPFFTTRDSGTGLGLSVSYGIVRDHGGDIQ
VSSTPGAGTCFKIVLPGETETAQETPENVADL

Sequences:

>Translated_672_residues
MLKRFPIKAKLTFGALAPLFVAFFICSLAGLYIINEKIASQAQEKVRTDLNSAREVYRNELDRIREFIDLTATNPYNSSS
IVAGDHEIQALLRQRLYKKRLDILTAVDAKGRVLYRAHTPSLAGDLQKSYFVQQALKGVAVTGTALMGEQELAREGVALS
SRATIPLVSTPHARPRNDAIEKTGMVMVSAAPLRNHAGQIIGALYGAVLLNNNNALVDKIKEIVYEGVQFNGTDVGSATI
FLGDARIATNVRLTDGARAIGTRVSEEVYQRVIVEKKKWIRRAFVVNDWYFTAYEPILDLHGKAIGSLYVGMLEKPYTHM
QKSVNSILYMVLFVTSLIGLAVSGFIATLLARPIKELEKLAHRVARGERNLQMEVHTKDEVGDLADAFNLMTRALSRQEA
EIGLLHRALELKVEERTAQLSDKNRLLLQTQADLARAEKLADLGIVAAGVAHEINTPLAIIRGNAEVLEMCLPPEHPNHE
EVEIISMQTERMAKIVGNLLTFARQKSLNQREFMVHEILDDIVTQIRHQVPMDAISVQWEYDMNLGAVTGDTDQLRQVFS
NIILNAVQAMLPEGGTLRLTTRPHGPGNGCEVEIRDTGKGIPAEHLEKIFTPFFTTRDSGTGLGLSVSYGIVRDHGGDIQ
VSSTPGAGTCFKIVLPGETETAQETPENVADL
>Mature_672_residues
MLKRFPIKAKLTFGALAPLFVAFFICSLAGLYIINEKIASQAQEKVRTDLNSAREVYRNELDRIREFIDLTATNPYNSSS
IVAGDHEIQALLRQRLYKKRLDILTAVDAKGRVLYRAHTPSLAGDLQKSYFVQQALKGVAVTGTALMGEQELAREGVALS
SRATIPLVSTPHARPRNDAIEKTGMVMVSAAPLRNHAGQIIGALYGAVLLNNNNALVDKIKEIVYEGVQFNGTDVGSATI
FLGDARIATNVRLTDGARAIGTRVSEEVYQRVIVEKKKWIRRAFVVNDWYFTAYEPILDLHGKAIGSLYVGMLEKPYTHM
QKSVNSILYMVLFVTSLIGLAVSGFIATLLARPIKELEKLAHRVARGERNLQMEVHTKDEVGDLADAFNLMTRALSRQEA
EIGLLHRALELKVEERTAQLSDKNRLLLQTQADLARAEKLADLGIVAAGVAHEINTPLAIIRGNAEVLEMCLPPEHPNHE
EVEIISMQTERMAKIVGNLLTFARQKSLNQREFMVHEILDDIVTQIRHQVPMDAISVQWEYDMNLGAVTGDTDQLRQVFS
NIILNAVQAMLPEGGTLRLTTRPHGPGNGCEVEIRDTGKGIPAEHLEKIFTPFFTTRDSGTGLGLSVSYGIVRDHGGDIQ
VSSTPGAGTCFKIVLPGETETAQETPENVADL

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=35.042735042735, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788549, Length=232, Percent_Identity=36.2068965517241, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1790300, Length=247, Percent_Identity=33.1983805668016, Blast_Score=119, Evalue=8e-28,
Organism=Escherichia coli, GI87081816, Length=326, Percent_Identity=28.2208588957055, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI145693157, Length=226, Percent_Identity=31.4159292035398, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789149, Length=357, Percent_Identity=25.4901960784314, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=29.5154185022026, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87082128, Length=221, Percent_Identity=28.0542986425339, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786912, Length=221, Percent_Identity=26.6968325791855, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1788393, Length=326, Percent_Identity=24.5398773006135, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI48994928, Length=238, Percent_Identity=27.3109243697479, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1788713, Length=225, Percent_Identity=24.8888888888889, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 74083; Mature: 74083

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRFPIKAKLTFGALAPLFVAFFICSLAGLYIINEKIASQAQEKVRTDLNSAREVYRNE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRIREFIDLTATNPYNSSSIVAGDHEIQALLRQRLYKKRLDILTAVDAKGRVLYRAHTP
HHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCC
SLAGDLQKSYFVQQALKGVAVTGTALMGEQELAREGVALSSRATIPLVSTPHARPRNDAI
CHHHHHHHHHHHHHHHCCEEEECHHHCCHHHHHHCCCEECCCCCEEEEECCCCCCCCHHH
EKTGMVMVSAAPLRNHAGQIIGALYGAVLLNNNNALVDKIKEIVYEGVQFNGTDVGSATI
HHCCEEEEECCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
FLGDARIATNVRLTDGARAIGTRVSEEVYQRVIVEKKKWIRRAFVVNDWYFTAYEPILDL
EEECCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHH
HGKAIGSLYVGMLEKPYTHMQKSVNSILYMVLFVTSLIGLAVSGFIATLLARPIKELEKL
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHRVARGERNLQMEVHTKDEVGDLADAFNLMTRALSRQEAEIGLLHRALELKVEERTAQL
HHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDKNRLLLQTQADLARAEKLADLGIVAAGVAHEINTPLAIIRGNAEVLEMCLPPEHPNHE
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCC
EVEIISMQTERMAKIVGNLLTFARQKSLNQREFMVHEILDDIVTQIRHQVPMDAISVQWE
CEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YDMNLGAVTGDTDQLRQVFSNIILNAVQAMLPEGGTLRLTTRPHGPGNGCEVEIRDTGKG
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCC
IPAEHLEKIFTPFFTTRDSGTGLGLSVSYGIVRDHGGDIQVSSTPGAGTCFKIVLPGETE
CCHHHHHHHHHHHHCCCCCCCCCEEEEEECEEEECCCEEEEECCCCCCCEEEEEECCCCC
TAQETPENVADL
CHHCCCHHHCCC
>Mature Secondary Structure
MLKRFPIKAKLTFGALAPLFVAFFICSLAGLYIINEKIASQAQEKVRTDLNSAREVYRNE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDRIREFIDLTATNPYNSSSIVAGDHEIQALLRQRLYKKRLDILTAVDAKGRVLYRAHTP
HHHHHHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCC
SLAGDLQKSYFVQQALKGVAVTGTALMGEQELAREGVALSSRATIPLVSTPHARPRNDAI
CHHHHHHHHHHHHHHHCCEEEECHHHCCHHHHHHCCCEECCCCCEEEEECCCCCCCCHHH
EKTGMVMVSAAPLRNHAGQIIGALYGAVLLNNNNALVDKIKEIVYEGVQFNGTDVGSATI
HHCCEEEEECCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCCCCCCCCEEE
FLGDARIATNVRLTDGARAIGTRVSEEVYQRVIVEKKKWIRRAFVVNDWYFTAYEPILDL
EEECCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHH
HGKAIGSLYVGMLEKPYTHMQKSVNSILYMVLFVTSLIGLAVSGFIATLLARPIKELEKL
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHRVARGERNLQMEVHTKDEVGDLADAFNLMTRALSRQEAEIGLLHRALELKVEERTAQL
HHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SDKNRLLLQTQADLARAEKLADLGIVAAGVAHEINTPLAIIRGNAEVLEMCLPPEHPNHE
CCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCC
EVEIISMQTERMAKIVGNLLTFARQKSLNQREFMVHEILDDIVTQIRHQVPMDAISVQWE
CEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YDMNLGAVTGDTDQLRQVFSNIILNAVQAMLPEGGTLRLTTRPHGPGNGCEVEIRDTGKG
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEECCCCC
IPAEHLEKIFTPFFTTRDSGTGLGLSVSYGIVRDHGGDIQVSSTPGAGTCFKIVLPGETE
CCHHHHHHHHHHHHCCCCCCCCCEEEEEECEEEECCCEEEEECCCCCCCEEEEEECCCCC
TAQETPENVADL
CHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]