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The map label for this gene is nifK [H]

Identifier: 197118458

GI number: 197118458

Start: 2400000

End: 2401463

Strand: Direct

Name: nifK [H]

Synonym: Gbem_2076

Alternate gene names: 197118458

Gene position: 2400000-2401463 (Clockwise)

Preceding gene: 197118457

Following gene: 197118459

Centisome position: 52.0

GC content: 61.68

Gene sequence:

>1464_bases
ATGACTGCAGAAGCTGCCGTGAAATACGTCACTGAGATTCCCGAAGAGGAAGTGAAACGGGTCTCTGCGTGGATCAATAC
CGAGGAGTACAAGGAGAAGAACTTCGCCCGCCAGGCGCTGGTAATCAACCCGCCGCACGCCTGCCAGCCGCTGGGCGCGG
AGCTTGCCGCGCACGGCTTCGAGGGGACGCTCCCCTTCGTGCACGGATCGCAAGGGTGCGCCTCGTACTACCGCTCCACC
TTCAACCGCCATTTCCGCGAGCCGGCTCCGGCCGTGTCCGACTCCATGACCGAGGACGGCGCGGTGTTCGGGGGGCAGAA
CAACCTGCACGAGGCACTGGAGAACGCCTACACCATCTATCAGCCCAAGATGATGGCGGTCTTCACCTCCTGCATGCCGG
AGGTCATCGGCGACGACCTGACTGCGTTCATCAAGAACGCGAGGAACAAGGGGATCGTGCCGCAGGACTACCCGCTCCCC
TACGCCAACACCCCGAGCTTCAACGGCTCGCACGTTCACGGCTACGACGCGATGCTCCTCTCCATCCTGCAGACGCTGAC
CGAGGGGAAGAAAGTCGAAGGGCGCTGCACCGGGAAGCTGAACCTGATCCCCGGCTTTGACTGCAACACCGGGAACTACC
GGGAGTACAAGAGGATCCTGAAGGAATTCGGCATCCCCTACACGCTTTTGGCCGACATCTCCGACACCTTCGATTCGCCC
CTGGACGGCACCTACCGTCCCTACCCGGGCGGCACCAAGCTGGAGGACGCGGCGGATTCCATCAACGGCAAGGCAACTTT
GACCGTGGCGCCTTTTTCCAGCGCCAAGACCTTTACCTGGATCAAGGACAACTACTCCGGGACCCACGTCTCGCTGCCGA
CCCCGTTCGGGGTTGCTAAGACCGACGCCCTGCTTCTGAAGCTCTCCGAGCTCTTCGGCAAGCCGGTGCCCGAGTCTCTC
AAGGCCGAGCGCGGCTACGCCGTCGACGCCATGACCGACGCGCACCAGTACATTCACGGCAAGAAGTTCGCCCTCTACGG
GGATCCGGATTACCTGATCGGGTATGTCTCCTTCCTGTTGGAGATGGGTGCCAAGCCGTACCACATTCTTTGCAGCAAGG
GGAACAAGAAGGTCGAGAAAGAGCTCCAGGCGCTTCTGGACGCGTCCCCGGACGGCAAAGGGTGCAAGATCTACATGGGC
AAGGACCTCTGGCACATGAGGAGTCTGCTGATGACCGACCCGGTCGACGCGATGATCGGCGACACGCACGGGAAGTTCGC
CGCGCGCGACGCAGGGATCCCGCTCTTCAGGTTCGGCTTCCCGGTCTTCGACCGCGTCAACCTGCATCGCTCGCCCCTCA
TCGGCTACCAGGGCGCCATCAACATGCTGACCGCCATCTGCAACAAGTTCATCGAAATCCGTGACGAGACCTGCGAGGAC
CGGCACTTCGAGATGATGAGATAA

Upstream 100 bases:

>100_bases
TAGGCAGTACCTGTTTTTGTTTTTTTCTCAATCTCAATCTTAATCTCAATCTTGATTTGAGCCGTGCGACGGATTAGTCG
CCGACGAAAGGAGCACTACT

Downstream 100 bases:

>100_bases
AAACCCAAAACCATTAGCAAGGAGATTGGGAGGAAATCTGAGAAAGCAAAGGCAAGGAGAGAAGCACAAGAAGGTTTAAA
ACTTAAGTCTTTGTCTTTTC

Product: nitrogenase molybdenum-iron protein subunit beta

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MTAEAAVKYVTEIPEEEVKRVSAWINTEEYKEKNFARQALVINPPHACQPLGAELAAHGFEGTLPFVHGSQGCASYYRST
FNRHFREPAPAVSDSMTEDGAVFGGQNNLHEALENAYTIYQPKMMAVFTSCMPEVIGDDLTAFIKNARNKGIVPQDYPLP
YANTPSFNGSHVHGYDAMLLSILQTLTEGKKVEGRCTGKLNLIPGFDCNTGNYREYKRILKEFGIPYTLLADISDTFDSP
LDGTYRPYPGGTKLEDAADSINGKATLTVAPFSSAKTFTWIKDNYSGTHVSLPTPFGVAKTDALLLKLSELFGKPVPESL
KAERGYAVDAMTDAHQYIHGKKFALYGDPDYLIGYVSFLLEMGAKPYHILCSKGNKKVEKELQALLDASPDGKGCKIYMG
KDLWHMRSLLMTDPVDAMIGDTHGKFAARDAGIPLFRFGFPVFDRVNLHRSPLIGYQGAINMLTAICNKFIEIRDETCED
RHFEMMR

Sequences:

>Translated_487_residues
MTAEAAVKYVTEIPEEEVKRVSAWINTEEYKEKNFARQALVINPPHACQPLGAELAAHGFEGTLPFVHGSQGCASYYRST
FNRHFREPAPAVSDSMTEDGAVFGGQNNLHEALENAYTIYQPKMMAVFTSCMPEVIGDDLTAFIKNARNKGIVPQDYPLP
YANTPSFNGSHVHGYDAMLLSILQTLTEGKKVEGRCTGKLNLIPGFDCNTGNYREYKRILKEFGIPYTLLADISDTFDSP
LDGTYRPYPGGTKLEDAADSINGKATLTVAPFSSAKTFTWIKDNYSGTHVSLPTPFGVAKTDALLLKLSELFGKPVPESL
KAERGYAVDAMTDAHQYIHGKKFALYGDPDYLIGYVSFLLEMGAKPYHILCSKGNKKVEKELQALLDASPDGKGCKIYMG
KDLWHMRSLLMTDPVDAMIGDTHGKFAARDAGIPLFRFGFPVFDRVNLHRSPLIGYQGAINMLTAICNKFIEIRDETCED
RHFEMMR
>Mature_486_residues
TAEAAVKYVTEIPEEEVKRVSAWINTEEYKEKNFARQALVINPPHACQPLGAELAAHGFEGTLPFVHGSQGCASYYRSTF
NRHFREPAPAVSDSMTEDGAVFGGQNNLHEALENAYTIYQPKMMAVFTSCMPEVIGDDLTAFIKNARNKGIVPQDYPLPY
ANTPSFNGSHVHGYDAMLLSILQTLTEGKKVEGRCTGKLNLIPGFDCNTGNYREYKRILKEFGIPYTLLADISDTFDSPL
DGTYRPYPGGTKLEDAADSINGKATLTVAPFSSAKTFTWIKDNYSGTHVSLPTPFGVAKTDALLLKLSELFGKPVPESLK
AERGYAVDAMTDAHQYIHGKKFALYGDPDYLIGYVSFLLEMGAKPYHILCSKGNKKVEKELQALLDASPDGKGCKIYMGK
DLWHMRSLLMTDPVDAMIGDTHGKFAARDAGIPLFRFGFPVFDRVNLHRSPLIGYQGAINMLTAICNKFIEIRDETCEDR
HFEMMR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54043; Mature: 53912

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00699 NITROGENASE_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAEAAVKYVTEIPEEEVKRVSAWINTEEYKEKNFARQALVINPPHACQPLGAELAAHGF
CCHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHCCC
EGTLPFVHGSQGCASYYRSTFNRHFREPAPAVSDSMTEDGAVFGGQNNLHEALENAYTIY
CCCCCCEECCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEECCCCHHHHHHHCCEEEC
QPKMMAVFTSCMPEVIGDDLTAFIKNARNKGIVPQDYPLPYANTPSFNGSHVHGYDAMLL
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SILQTLTEGKKVEGRCTGKLNLIPGFDCNTGNYREYKRILKEFGIPYTLLADISDTFDSP
HHHHHHHCCCCCCCEECCEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHCCC
LDGTYRPYPGGTKLEDAADSINGKATLTVAPFSSAKTFTWIKDNYSGTHVSLPTPFGVAK
CCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCEEECCCCCCCHH
TDALLLKLSELFGKPVPESLKAERGYAVDAMTDAHQYIHGKKFALYGDPDYLIGYVSFLL
HHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
EMGAKPYHILCSKGNKKVEKELQALLDASPDGKGCKIYMGKDLWHMRSLLMTDPVDAMIG
HCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHC
DTHGKFAARDAGIPLFRFGFPVFDRVNLHRSPLIGYQGAINMLTAICNKFIEIRDETCED
CCCCCCCHHCCCCCHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
RHFEMMR
CHHHHCC
>Mature Secondary Structure 
TAEAAVKYVTEIPEEEVKRVSAWINTEEYKEKNFARQALVINPPHACQPLGAELAAHGF
CHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHCCC
EGTLPFVHGSQGCASYYRSTFNRHFREPAPAVSDSMTEDGAVFGGQNNLHEALENAYTIY
CCCCCCEECCHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCEECCCCHHHHHHHCCEEEC
QPKMMAVFTSCMPEVIGDDLTAFIKNARNKGIVPQDYPLPYANTPSFNGSHVHGYDAMLL
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
SILQTLTEGKKVEGRCTGKLNLIPGFDCNTGNYREYKRILKEFGIPYTLLADISDTFDSP
HHHHHHHCCCCCCCEECCEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHHHCCC
LDGTYRPYPGGTKLEDAADSINGKATLTVAPFSSAKTFTWIKDNYSGTHVSLPTPFGVAK
CCCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCEEEEEECCCCCCEEECCCCCCCHH
TDALLLKLSELFGKPVPESLKAERGYAVDAMTDAHQYIHGKKFALYGDPDYLIGYVSFLL
HHHHHHHHHHHHCCCCCHHHHHCCCCEEHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHH
EMGAKPYHILCSKGNKKVEKELQALLDASPDGKGCKIYMGKDLWHMRSLLMTDPVDAMIG
HCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHC
DTHGKFAARDAGIPLFRFGFPVFDRVNLHRSPLIGYQGAINMLTAICNKFIEIRDETCED
CCCCCCCHHCCCCCHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
RHFEMMR
CHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]