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The map label for this gene is nifEN [H]

Identifier: 197118459

GI number: 197118459

Start: 2401616

End: 2404372

Strand: Direct

Name: nifEN [H]

Synonym: Gbem_2077

Alternate gene names: 197118459

Gene position: 2401616-2404372 (Clockwise)

Preceding gene: 197118458

Following gene: 197118460

Centisome position: 52.04

GC content: 62.86

Gene sequence:

>2757_bases
ATGGCGAAGCCAGACTGGTACGACACAACTGATTGCGACACTCACGATGCCGGGGCTCCTAAGTTCTGCAAGAAGTCGGA
ACCGGGCGAGGGGACGGAACGAAGCTGCGCCTACGACGGCGCCCGCGTGGTTCTCATGCCCATCACCGACGTCATCCACC
TGGTGCACGGCCCCATCGCCTGCGCCGGAAACTCCTGGGACAACCGCGGGGCACGCTCCTCGGATTCCCAGATCTACCGC
CGCGGCTTCACCACGGAGATGCTGCAAAACGACGTGATCTTCGGCGGCGAAAAGAAGCTCTACCGCGCCATCCAGGAACT
GGCGGCGCGCTACCCGGAGGCAAAGGCGATCTTCGTCTACGCCACCTGCGTTACCTCCATGACCGGCGACGACATCGAGG
CGGTCTGCAAAGCGGCCCAGGAGAAGGTGAGCGTCCCGGTGATCCCGGTGAATACGCCTGGCTTCATCGGCGACAAGAAC
ATCGGCAACCGCCTGGCCGGCGAGACCCTCTTCAAGTACGTAATTGGCACCGCGGAACCGGAATTCACCACGGATTACGA
CATAAACCTGATCGGCGAGTACAACATCGCCGGCGACCTCTGGGGGATGCTGCCGCTCTTCGACCGGCTCGGGATCCGCG
TCCTCTCCTGCATCAGCGGCGACGCCAAGTTCGAGGAGCTGCGCTACGCGCACCGCGCCAAGCTGAACATCATCGTCTGC
TCCAAGAGCCTCACCAACCTCGCCAAGAAGATGCAGAAGGTGTACGGCATCCCGTACCTGGAGGAATCCTTCTACGGCAT
GACGGATGTGGCGAAGGCGCTGCGGGACATAGCGCGGGAGCTGGACGACCTGGTGAACGGCCTGGAGAAGCGGGTGATGC
AGGACCGGGTGGAAAAGCTGGTCGCCGAGTGCGAGGAGAGCTGCCGCGCCGAGCTTGCTCCTTATCGGGAGAGGCTGGCC
GGGAAGAAGGCGGTGCTCTTCACCGGGGGGGTGAAGACCTGGTCCATGGTGAACGCGCTGGCGGAGCTGGGGGTGGAGAT
TCTCGCAGCGGGAACGCAGAATTCGACGCTGGAGGATTTCTACCGCATGAAGGCGCTGATGCACGAGGATGCCTCCATCA
TCGCGGACACCAGTACGGCGGGGCTTCTTTCGGTCATGTACGAAAAGCTCCCCGACCTGATCGTCGCGGGGGGGAAGACC
AAGTTCCTGGCGCTGAAGACGAAGACCCCGTTTCTGGACATAAACCACGGGCGCTCCCATCCTTACGCGGGTTACGCCGG
AATGGTCACCTTCGCCAAACAGCTTGATCTCACGGTGAATAACCCGATCTGGCCGACCCTGAACGACAAGGCCCCCTGGG
AGAAGGACGCCGAGGCGCAGAAAGCGGATCTGGCCTACGCCGCCGGGCATGCCGCGCGCTTCGCCGCCGAAGAGATCAAG
GCCTCGCGGGTCAAGGTGCCGACCAAGAACGCCACGGTAAACCCGCAGAAGAACTCCCCCGCGCTGGGGGCGACGCTCGC
CTATCTCGGGATCGACCGGATGCTGGGGCTTTTGCACGGCGCCCAGGGATGCTCCACCTTCATCAGGCTGCAGCTCTCCC
GGCATTTCAAGGAATCCATCGCGCTCAACTCGACCAGCATGAGCGAGGAGACGGCCATCTTCGGCGGCTGGGACAACCTG
AGGGTCGGCCTGAACCGGGTCATGGAAAAGTTCAAGCCAGAGGTGGTGGGCGTGATGACCACCGGTTTGACCGAGACCAT
GGGGGACGACGTCAGGAGCGCCATCGTCAAGTTCCGGGAGGCGCATCCGGAGCACGATGCCATTCCCGTGATCCACGCCT
CGACCCCGGATTACTGCGGCTCGATGCAGGAAGGGTACGCCGCGGCGGTCGAGGCCATCGTGGCGACGGTGCCGGAAGGG
GGCATCGGAATACCTGGACAGGTAAGTATTCTGCCGGGTTGCCAGCTAACTCCTGCCGAGGTCGAGGAAATCGCGGGAAT
CTGCGAGGCGTTCGGGCTGGATCCGGTGGTTGTGCCGGACATCTCCAACGCGCTGGATGGGCACATCGACGCCACCGTCT
CGGCGCTCTCCGTAGGGGGAGCCACCATCGAGCGCATCAAGGCGGCGGGGCGGAGCGAAGCGACGCTATATTTCGGCGAT
TCGCTGGCCGAAGCAGCCGGGGTGCTCCAGGACAAGTTCGGCATCCCGAGTTACGGGTTCACCTCCATCACCGGGCTCAA
GGAGACGGACCTATTGATGGCCACGCTCTCGGCTCTTTCCGGACGGCCGATACCTGTGAAATTCCGGCGCTGGAGAAGCC
GCCTCATGGACGCCATGGTGGACAGCCACTACCAGTTCGGACAGAAAAAAGTGGCGCTGGCGCTGGAGGCGGACCACCTG
AAGGGGATGACACATTTCCTTGCCAGCCTCGGCTGCGAAATACAGGTCGCCATTGCTGCAACGAGGACGCGGGGCTTGGA
CCGCTTGCCGACGGAGAACGTATTCGTCGGGGACCTGGAGGACCTGGAACAGACCGCGGCGGGAGCCGACCTGTTAGTGG
CCAACTCCAACGGGCGCCAAGCGGCGAAGAAACTAGGGATCAATGCACACCTGAGAACCGGGATGCCCGTTTTCGACCGG
CTCGGGGCGCACCAAAAGATGTGGGTCGGCTACCAGGGGACGCTGAACCTGGTCTTCGAGGTCGCAAATATATTCCAAGC
CAATGCCAAGGAAGCGCAGAAACTGGCGCACAACTGA

Upstream 100 bases:

>100_bases
CAAGGAGAGAAGCACAAGAAGGTTTAAAACTTAAGTCTTTGTCTTTTCACAGAAGTCCTCAGATGTTCTCCTTGCTAATG
GTTTTAAAAAGGAGGCTCCA

Downstream 100 bases:

>100_bases
GAGGCAGAGAAGAGGCAGATTGAGATTAAGATTTAGATAAAGGCTAAAACCTTGGCTACAGAATCTTCTTTATCTCTATC
TTTATCTTTATCTCGCTTTT

Product: bifunctional nitrogenase molybdenum-cofactor biosynthesis protein NifE/NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 918; Mature: 917

Protein sequence:

>918_residues
MAKPDWYDTTDCDTHDAGAPKFCKKSEPGEGTERSCAYDGARVVLMPITDVIHLVHGPIACAGNSWDNRGARSSDSQIYR
RGFTTEMLQNDVIFGGEKKLYRAIQELAARYPEAKAIFVYATCVTSMTGDDIEAVCKAAQEKVSVPVIPVNTPGFIGDKN
IGNRLAGETLFKYVIGTAEPEFTTDYDINLIGEYNIAGDLWGMLPLFDRLGIRVLSCISGDAKFEELRYAHRAKLNIIVC
SKSLTNLAKKMQKVYGIPYLEESFYGMTDVAKALRDIARELDDLVNGLEKRVMQDRVEKLVAECEESCRAELAPYRERLA
GKKAVLFTGGVKTWSMVNALAELGVEILAAGTQNSTLEDFYRMKALMHEDASIIADTSTAGLLSVMYEKLPDLIVAGGKT
KFLALKTKTPFLDINHGRSHPYAGYAGMVTFAKQLDLTVNNPIWPTLNDKAPWEKDAEAQKADLAYAAGHAARFAAEEIK
ASRVKVPTKNATVNPQKNSPALGATLAYLGIDRMLGLLHGAQGCSTFIRLQLSRHFKESIALNSTSMSEETAIFGGWDNL
RVGLNRVMEKFKPEVVGVMTTGLTETMGDDVRSAIVKFREAHPEHDAIPVIHASTPDYCGSMQEGYAAAVEAIVATVPEG
GIGIPGQVSILPGCQLTPAEVEEIAGICEAFGLDPVVVPDISNALDGHIDATVSALSVGGATIERIKAAGRSEATLYFGD
SLAEAAGVLQDKFGIPSYGFTSITGLKETDLLMATLSALSGRPIPVKFRRWRSRLMDAMVDSHYQFGQKKVALALEADHL
KGMTHFLASLGCEIQVAIAATRTRGLDRLPTENVFVGDLEDLEQTAAGADLLVANSNGRQAAKKLGINAHLRTGMPVFDR
LGAHQKMWVGYQGTLNLVFEVANIFQANAKEAQKLAHN

Sequences:

>Translated_918_residues
MAKPDWYDTTDCDTHDAGAPKFCKKSEPGEGTERSCAYDGARVVLMPITDVIHLVHGPIACAGNSWDNRGARSSDSQIYR
RGFTTEMLQNDVIFGGEKKLYRAIQELAARYPEAKAIFVYATCVTSMTGDDIEAVCKAAQEKVSVPVIPVNTPGFIGDKN
IGNRLAGETLFKYVIGTAEPEFTTDYDINLIGEYNIAGDLWGMLPLFDRLGIRVLSCISGDAKFEELRYAHRAKLNIIVC
SKSLTNLAKKMQKVYGIPYLEESFYGMTDVAKALRDIARELDDLVNGLEKRVMQDRVEKLVAECEESCRAELAPYRERLA
GKKAVLFTGGVKTWSMVNALAELGVEILAAGTQNSTLEDFYRMKALMHEDASIIADTSTAGLLSVMYEKLPDLIVAGGKT
KFLALKTKTPFLDINHGRSHPYAGYAGMVTFAKQLDLTVNNPIWPTLNDKAPWEKDAEAQKADLAYAAGHAARFAAEEIK
ASRVKVPTKNATVNPQKNSPALGATLAYLGIDRMLGLLHGAQGCSTFIRLQLSRHFKESIALNSTSMSEETAIFGGWDNL
RVGLNRVMEKFKPEVVGVMTTGLTETMGDDVRSAIVKFREAHPEHDAIPVIHASTPDYCGSMQEGYAAAVEAIVATVPEG
GIGIPGQVSILPGCQLTPAEVEEIAGICEAFGLDPVVVPDISNALDGHIDATVSALSVGGATIERIKAAGRSEATLYFGD
SLAEAAGVLQDKFGIPSYGFTSITGLKETDLLMATLSALSGRPIPVKFRRWRSRLMDAMVDSHYQFGQKKVALALEADHL
KGMTHFLASLGCEIQVAIAATRTRGLDRLPTENVFVGDLEDLEQTAAGADLLVANSNGRQAAKKLGINAHLRTGMPVFDR
LGAHQKMWVGYQGTLNLVFEVANIFQANAKEAQKLAHN
>Mature_917_residues
AKPDWYDTTDCDTHDAGAPKFCKKSEPGEGTERSCAYDGARVVLMPITDVIHLVHGPIACAGNSWDNRGARSSDSQIYRR
GFTTEMLQNDVIFGGEKKLYRAIQELAARYPEAKAIFVYATCVTSMTGDDIEAVCKAAQEKVSVPVIPVNTPGFIGDKNI
GNRLAGETLFKYVIGTAEPEFTTDYDINLIGEYNIAGDLWGMLPLFDRLGIRVLSCISGDAKFEELRYAHRAKLNIIVCS
KSLTNLAKKMQKVYGIPYLEESFYGMTDVAKALRDIARELDDLVNGLEKRVMQDRVEKLVAECEESCRAELAPYRERLAG
KKAVLFTGGVKTWSMVNALAELGVEILAAGTQNSTLEDFYRMKALMHEDASIIADTSTAGLLSVMYEKLPDLIVAGGKTK
FLALKTKTPFLDINHGRSHPYAGYAGMVTFAKQLDLTVNNPIWPTLNDKAPWEKDAEAQKADLAYAAGHAARFAAEEIKA
SRVKVPTKNATVNPQKNSPALGATLAYLGIDRMLGLLHGAQGCSTFIRLQLSRHFKESIALNSTSMSEETAIFGGWDNLR
VGLNRVMEKFKPEVVGVMTTGLTETMGDDVRSAIVKFREAHPEHDAIPVIHASTPDYCGSMQEGYAAAVEAIVATVPEGG
IGIPGQVSILPGCQLTPAEVEEIAGICEAFGLDPVVVPDISNALDGHIDATVSALSVGGATIERIKAAGRSEATLYFGDS
LAEAAGVLQDKFGIPSYGFTSITGLKETDLLMATLSALSGRPIPVKFRRWRSRLMDAMVDSHYQFGQKKVALALEADHLK
GMTHFLASLGCEIQVAIAATRTRGLDRLPTENVFVGDLEDLEQTAAGADLLVANSNGRQAAKKLGINAHLRTGMPVFDRL
GAHQKMWVGYQGTLNLVFEVANIFQANAKEAQKLAHN

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 99604; Mature: 99473

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPDWYDTTDCDTHDAGAPKFCKKSEPGEGTERSCAYDGARVVLMPITDVIHLVHGPIA
CCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCEE
CAGNSWDNRGARSSDSQIYRRGFTTEMLQNDVIFGGEKKLYRAIQELAARYPEAKAIFVY
ECCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCEECCHHHHHHHHHHHHHHCCCCCEEEEE
ATCVTSMTGDDIEAVCKAAQEKVSVPVIPVNTPGFIGDKNIGNRLAGETLFKYVIGTAEP
EEHHHCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
EFTTDYDINLIGEYNIAGDLWGMLPLFDRLGIRVLSCISGDAKFEELRYAHRAKLNIIVC
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEEEE
SKSLTNLAKKMQKVYGIPYLEESFYGMTDVAKALRDIARELDDLVNGLEKRVMQDRVEKL
CHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAECEESCRAELAPYRERLAGKKAVLFTGGVKTWSMVNALAELGVEILAAGTQNSTLEDF
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCHHEEECCCCCCHHHHH
YRMKALMHEDASIIADTSTAGLLSVMYEKLPDLIVAGGKTKFLALKTKTPFLDINHGRSH
HHHHHHHHCCCCEEECCCHHHHHHHHHHHCCHHEEECCCEEEEEEECCCCEEECCCCCCC
PYAGYAGMVTFAKQLDLTVNNPIWPTLNDKAPWEKDAEAQKADLAYAAGHAARFAAEEIK
CCCCHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASRVKVPTKNATVNPQKNSPALGATLAYLGIDRMLGLLHGAQGCSTFIRLQLSRHFKESI
HHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ALNSTSMSEETAIFGGWDNLRVGLNRVMEKFKPEVVGVMTTGLTETMGDDVRSAIVKFRE
HCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
AHPEHDAIPVIHASTPDYCGSMQEGYAAAVEAIVATVPEGGIGIPGQVSILPGCQLTPAE
CCCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHH
VEEIAGICEAFGLDPVVVPDISNALDGHIDATVSALSVGGATIERIKAAGRSEATLYFGD
HHHHHHHHHHHCCCCEECCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECC
SLAEAAGVLQDKFGIPSYGFTSITGLKETDLLMATLSALSGRPIPVKFRRWRSRLMDAMV
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
DSHYQFGQKKVALALEADHLKGMTHFLASLGCEIQVAIAATRTRGLDRLPTENVFVGDLE
HHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCCEEECCHH
DLEQTAAGADLLVANSNGRQAAKKLGINAHLRTGMPVFDRLGAHQKMWVGYQGTLNLVFE
HHHHHHCCCEEEEECCCCHHHHHHCCCCCEECCCCCHHHHCCCCCEEEEECCHHHHHHHH
VANIFQANAKEAQKLAHN
HHHHHHCCHHHHHHHHCC
>Mature Secondary Structure 
AKPDWYDTTDCDTHDAGAPKFCKKSEPGEGTERSCAYDGARVVLMPITDVIHLVHGPIA
CCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCEE
CAGNSWDNRGARSSDSQIYRRGFTTEMLQNDVIFGGEKKLYRAIQELAARYPEAKAIFVY
ECCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCEECCHHHHHHHHHHHHHHCCCCCEEEEE
ATCVTSMTGDDIEAVCKAAQEKVSVPVIPVNTPGFIGDKNIGNRLAGETLFKYVIGTAEP
EEHHHCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
EFTTDYDINLIGEYNIAGDLWGMLPLFDRLGIRVLSCISGDAKFEELRYAHRAKLNIIVC
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCEEEEEEE
SKSLTNLAKKMQKVYGIPYLEESFYGMTDVAKALRDIARELDDLVNGLEKRVMQDRVEKL
CHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAECEESCRAELAPYRERLAGKKAVLFTGGVKTWSMVNALAELGVEILAAGTQNSTLEDF
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCHHEEECCCCCCHHHHH
YRMKALMHEDASIIADTSTAGLLSVMYEKLPDLIVAGGKTKFLALKTKTPFLDINHGRSH
HHHHHHHHCCCCEEECCCHHHHHHHHHHHCCHHEEECCCEEEEEEECCCCEEECCCCCCC
PYAGYAGMVTFAKQLDLTVNNPIWPTLNDKAPWEKDAEAQKADLAYAAGHAARFAAEEIK
CCCCHHHHHHHHHHHCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ASRVKVPTKNATVNPQKNSPALGATLAYLGIDRMLGLLHGAQGCSTFIRLQLSRHFKESI
HHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ALNSTSMSEETAIFGGWDNLRVGLNRVMEKFKPEVVGVMTTGLTETMGDDVRSAIVKFRE
HCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCEEEHHHHCCHHHHHHHHHHHHHHHHH
AHPEHDAIPVIHASTPDYCGSMQEGYAAAVEAIVATVPEGGIGIPGQVSILPGCQLTPAE
CCCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHH
VEEIAGICEAFGLDPVVVPDISNALDGHIDATVSALSVGGATIERIKAAGRSEATLYFGD
HHHHHHHHHHHCCCCEECCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCEEEEECC
SLAEAAGVLQDKFGIPSYGFTSITGLKETDLLMATLSALSGRPIPVKFRRWRSRLMDAMV
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
DSHYQFGQKKVALALEADHLKGMTHFLASLGCEIQVAIAATRTRGLDRLPTENVFVGDLE
HHHHHCCCCEEEEEEEHHHHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCCEEECCHH
DLEQTAAGADLLVANSNGRQAAKKLGINAHLRTGMPVFDRLGAHQKMWVGYQGTLNLVFE
HHHHHHCCCEEEEECCCCHHHHHHCCCCCEECCCCCHHHHCCCCCEEEEECCHHHHHHHH
VANIFQANAKEAQKLAHN
HHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]