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The map label for this gene is nifD [H]

Identifier: 197118457

GI number: 197118457

Start: 2398346

End: 2399809

Strand: Direct

Name: nifD [H]

Synonym: Gbem_2075

Alternate gene names: 197118457

Gene position: 2398346-2399809 (Clockwise)

Preceding gene: 197118456

Following gene: 197118458

Centisome position: 51.97

GC content: 60.86

Gene sequence:

>1464_bases
ATGCTGAATAAGGAGACTTACATGTCTGAGGCACCAATAAAGAAGGTAGAAGGAATTACAAAGGAATCTACCCAGGCGAT
GATCGACCACGCCCTGGAGATCTATCCGGAGAAGGCCAAGAAGAAGAGGGCGCCGCATCTCGCCCCCAACGAGGGGGAAG
GGGCCTGCGTCAAGAGTAACCGCAAGACCGTCCCCGGCATCATGAGCGCCCGCGGCTGTGCCTACGCAGGTGCCAAAGGG
GTGGTCTGGGGACCGATCCGTGACATGGTGCACGTCTCCCACGGCCCGGTCGGCTGCGGCTGGTACTCCTGGGGTACCCG
CCGCAACCTGGTCACCGGCGTGAACGGCGTCAGCCAGTTCGCCATGCAGTTCACCTCCGATTTCCAGGAGAAGGACATCG
TCTACGGCGGCGACAAGAAGCTGAAGACACTTCTGGCCGAGGCGCACGACCTGTTCCCACTGGCGAAGGGGATCTCGGTC
CTCTCCGAGTGCCCGGTCGGCCTCATCGGCGACGACATAAACTCGGTAGCGAAGGTCGCCTCGAAGGAACTGGACATCCC
GGTCATCCCCTGCAACTGCGAGGGGTTCCGCGGCGTGTCCCAGTCGCTGGGCCATCACATCTCCAACGACACCATCCGCG
ACCACATCATCGGCACCCGGGAATTCGCCGAGCCCGAGACCCCGTACGACATCGCCCTCATCGGCGACTACAACATCGGC
GGCGACGTCTGGAGCGTTAAACCTCTGCTTGAAGAAATAGGGTTGAACGTCAAGGCGGTATGGACCGGCGACGGCGAATT
GGAAAAGATCGCCGCCACGCACAAGGTGAAGCTGAACCTGATCCACTGCTACCGTTCCATGAACTATATGTGCCGCGTCA
TGGAAGAGAAGTACGGCATCCCCTGGCTCGAGTTCAACTTCTTTGGCCCGACCAAGATCCGCGAGAGCCTGAGGAAGATC
GCCGAGTACTTCGACGACAACATCAAGGAGAAGGTGGAAAAGGCGATCGCTAAGTACGACCCCATCATGCAGGCGGTGAT
CGACGAGTACCGTCCGCGCTTGGAAGGGAAGAAGGTGATGCTCTACGTCGGCGGCCTTCGCCCCCGTCACACCGTTAACG
CCTACGCGGACCTCGGCATGACCGTGGTCGGTTCCGGTTACGAGTTCGCCCACGGCGACGACTACGAGAGGACCTCCCCC
GAGATGCCGGAAGCGACCGTCATCTACGACGACGCCTCCGAGCACGAGCTGGAGCAGTTCGTGGATGAGCTTCGTCCCGA
CCTGGTCGGCTCCGGCATCAAAGAGAAGTACCTGTTCCAGAAGATGGGCATCCCGTTCCGCCAGATGCACAGCTGGGACT
ACTCCGGCCCGTACCACGCCTACAACGGCTTCCCGATCTTCGCCCGCGACGTCGACATGGCCGTCAACAGCCCGACCTGG
AAGCTGGTGAAGGCGCCCTTTTAG

Upstream 100 bases:

>100_bases
GGCCGAGGCTGCAGCGGTTTAAATCACCCACCCCCTGACCCCCTCCCGCCTACGCGAGCCGTAGCGCTTCGGCGCGCAGG
CCCTTCGAGGGAGGGGGGAC

Downstream 100 bases:

>100_bases
GGCGCAGATTAAGAGATGAACCAGATAAAGGTTAAGTAAAGGCAGTAGGAACAGCAGATAAAGCTTGAGATAAAGATTAA
GTAAAGGCAGTAGGCAGTAC

Product: nitrogenase molybdenum-iron protein subunit alpha

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MLNKETYMSEAPIKKVEGITKESTQAMIDHALEIYPEKAKKKRAPHLAPNEGEGACVKSNRKTVPGIMSARGCAYAGAKG
VVWGPIRDMVHVSHGPVGCGWYSWGTRRNLVTGVNGVSQFAMQFTSDFQEKDIVYGGDKKLKTLLAEAHDLFPLAKGISV
LSECPVGLIGDDINSVAKVASKELDIPVIPCNCEGFRGVSQSLGHHISNDTIRDHIIGTREFAEPETPYDIALIGDYNIG
GDVWSVKPLLEEIGLNVKAVWTGDGELEKIAATHKVKLNLIHCYRSMNYMCRVMEEKYGIPWLEFNFFGPTKIRESLRKI
AEYFDDNIKEKVEKAIAKYDPIMQAVIDEYRPRLEGKKVMLYVGGLRPRHTVNAYADLGMTVVGSGYEFAHGDDYERTSP
EMPEATVIYDDASEHELEQFVDELRPDLVGSGIKEKYLFQKMGIPFRQMHSWDYSGPYHAYNGFPIFARDVDMAVNSPTW
KLVKAPF

Sequences:

>Translated_487_residues
MLNKETYMSEAPIKKVEGITKESTQAMIDHALEIYPEKAKKKRAPHLAPNEGEGACVKSNRKTVPGIMSARGCAYAGAKG
VVWGPIRDMVHVSHGPVGCGWYSWGTRRNLVTGVNGVSQFAMQFTSDFQEKDIVYGGDKKLKTLLAEAHDLFPLAKGISV
LSECPVGLIGDDINSVAKVASKELDIPVIPCNCEGFRGVSQSLGHHISNDTIRDHIIGTREFAEPETPYDIALIGDYNIG
GDVWSVKPLLEEIGLNVKAVWTGDGELEKIAATHKVKLNLIHCYRSMNYMCRVMEEKYGIPWLEFNFFGPTKIRESLRKI
AEYFDDNIKEKVEKAIAKYDPIMQAVIDEYRPRLEGKKVMLYVGGLRPRHTVNAYADLGMTVVGSGYEFAHGDDYERTSP
EMPEATVIYDDASEHELEQFVDELRPDLVGSGIKEKYLFQKMGIPFRQMHSWDYSGPYHAYNGFPIFARDVDMAVNSPTW
KLVKAPF
>Mature_487_residues
MLNKETYMSEAPIKKVEGITKESTQAMIDHALEIYPEKAKKKRAPHLAPNEGEGACVKSNRKTVPGIMSARGCAYAGAKG
VVWGPIRDMVHVSHGPVGCGWYSWGTRRNLVTGVNGVSQFAMQFTSDFQEKDIVYGGDKKLKTLLAEAHDLFPLAKGISV
LSECPVGLIGDDINSVAKVASKELDIPVIPCNCEGFRGVSQSLGHHISNDTIRDHIIGTREFAEPETPYDIALIGDYNIG
GDVWSVKPLLEEIGLNVKAVWTGDGELEKIAATHKVKLNLIHCYRSMNYMCRVMEEKYGIPWLEFNFFGPTKIRESLRKI
AEYFDDNIKEKVEKAIAKYDPIMQAVIDEYRPRLEGKKVMLYVGGLRPRHTVNAYADLGMTVVGSGYEFAHGDDYERTSP
EMPEATVIYDDASEHELEQFVDELRPDLVGSGIKEKYLFQKMGIPFRQMHSWDYSGPYHAYNGFPIFARDVDMAVNSPTW
KLVKAPF

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR010143
- InterPro:   IPR000318
- InterPro:   IPR005972 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 54512; Mature: 54512

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKETYMSEAPIKKVEGITKESTQAMIDHALEIYPEKAKKKRAPHLAPNEGEGACVKSN
CCCCHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEECCC
RKTVPGIMSARGCAYAGAKGVVWGPIRDMVHVSHGPVGCGWYSWGTRRNLVTGVNGVSQF
CCCCCCHHHCCCCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHH
AMQFTSDFQEKDIVYGGDKKLKTLLAEAHDLFPLAKGISVLSECPVGLIGDDINSVAKVA
HHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
SKELDIPVIPCNCEGFRGVSQSLGHHISNDTIRDHIIGTREFAEPETPYDIALIGDYNIG
HHCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCEEEEEEECCCCC
GDVWSVKPLLEEIGLNVKAVWTGDGELEKIAATHKVKLNLIHCYRSMNYMCRVMEEKYGI
CCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHCCC
PWLEFNFFGPTKIRESLRKIAEYFDDNIKEKVEKAIAKYDPIMQAVIDEYRPRLEGKKVM
CEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LYVGGLRPRHTVNAYADLGMTVVGSGYEFAHGDDYERTSPEMPEATVIYDDASEHELEQF
EEECCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
VDELRPDLVGSGIKEKYLFQKMGIPFRQMHSWDYSGPYHAYNGFPIFARDVDMAVNSPTW
HHHHCHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEECCCHHCCCCCE
KLVKAPF
EEEECCC
>Mature Secondary Structure
MLNKETYMSEAPIKKVEGITKESTQAMIDHALEIYPEKAKKKRAPHLAPNEGEGACVKSN
CCCCHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCEECCC
RKTVPGIMSARGCAYAGAKGVVWGPIRDMVHVSHGPVGCGWYSWGTRRNLVTGVNGVSQF
CCCCCCHHHCCCCCCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCCCCCHHHCCHHHHHH
AMQFTSDFQEKDIVYGGDKKLKTLLAEAHDLFPLAKGISVLSECPVGLIGDDINSVAKVA
HHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHH
SKELDIPVIPCNCEGFRGVSQSLGHHISNDTIRDHIIGTREFAEPETPYDIALIGDYNIG
HHCCCCEEEECCCCCCCHHHHHHHHHCCCCHHHHHHCCCHHCCCCCCCEEEEEEECCCCC
GDVWSVKPLLEEIGLNVKAVWTGDGELEKIAATHKVKLNLIHCYRSMNYMCRVMEEKYGI
CCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHCCC
PWLEFNFFGPTKIRESLRKIAEYFDDNIKEKVEKAIAKYDPIMQAVIDEYRPRLEGKKVM
CEEEEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
LYVGGLRPRHTVNAYADLGMTVVGSGYEFAHGDDYERTSPEMPEATVIYDDASEHELEQF
EEECCCCCCCHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
VDELRPDLVGSGIKEKYLFQKMGIPFRQMHSWDYSGPYHAYNGFPIFARDVDMAVNSPTW
HHHHCHHHHCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEECCCHHCCCCCE
KLVKAPF
EEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2644218; 3344210; 3863780; 649581; 9163420; 9063865; 11170380; 11951047; 12215645 [H]