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The map label for this gene is nifH [H]

Identifier: 197118456

GI number: 197118456

Start: 2397398

End: 2398267

Strand: Direct

Name: nifH [H]

Synonym: Gbem_2074

Alternate gene names: 197118456

Gene position: 2397398-2398267 (Clockwise)

Preceding gene: 197118455

Following gene: 197118457

Centisome position: 51.95

GC content: 61.72

Gene sequence:

>870_bases
ATGAGACAGATCGCGATTTACGGCAAGGGCGGCATCGGCAAATCCACCACTACCCAGAACACCGTGGCAGGCCTCGCCTC
CATCGGCAAGAAGGTCATGATCGTTGGCTGCGACCCTAAGGCCGACTCCACCCGTCTCATCCTGCACGCCAAGGCACAGT
CGACCGTTATGGACCTGGTCCGCGAGCTGGGCACGGTAGAGGACCTGGAGCTTGAGGACGTCATGAAGGTAGGTTACGGC
GATGTGAAATGCGTCGAGTCCGGCGGACCGGAGCCGGGCGTCGGCTGTGCCGGCCGCGGCGTCATCACCGCCATCAACTT
CCTGGAAGAAAACGGCGCCTACACCCCCGACCTCGACTTCGTCTTCTACGATGTTCTGGGCGACGTCGTCTGCGGCGGTT
TCGCCATGCCGATCCGCGAAGGGAAGGCCGAGGAGATCTACATCGTCTGCTCCGGCGAGATGATGGCCATGTACGCCGCC
AACAACATCGCCAAGGGGATCCTGAAGTACGCTTCCTCCGGCAAAGTCAGGCTGGCCGGCCTCATCTGCAACGCGAGGAA
GACGGACAAGGAATACGAGTTGATCGACGCGCTGGCCAAGAAGCTCGGCACCCAGATGATCCACTTCGTTCCCCGCGACA
ACCAGGTGCAGCGCGCCGAACTGCGCAGGATGACCGTCATTGAGTACTCCCCCGACCATCCGCAGGCCAACGAGTACCGC
TCCCTGGCCCAGAAGATCGCCGACAACAAGATGCTGGTGGTCCCGACCCCGCTTGAGATGGAAGAGCTGGAAGACCTCTT
GATGGAGTTCGGCATCATGGAGGCCGAGGACGAGTCGATCGTGGGCGTGGCCGAGGCTGCAGCGGTTTAA

Upstream 100 bases:

>100_bases
ATCATGCCAGGTGCCAGGATTGCCGGACAGGCCGGCAACGGCGACCAAGCGGTTCTGCCTGAGGGCGGAAGCGGTCAGTG
GGGCAAAAAGGAGAGGAACA

Downstream 100 bases:

>100_bases
ATCACCCACCCCCTGACCCCCTCCCGCCTACGCGAGCCGTAGCGCTTCGGCGCGCAGGCCCTTCGAGGGAGGGGGGACAT
GCTGAATAAGGAGACTTACA

Product: nitrogenase iron protein

Products: NA

Alternate protein names: Nitrogenase Fe protein 2; Nitrogenase component II; Nitrogenase reductase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MRQIAIYGKGGIGKSTTTQNTVAGLASIGKKVMIVGCDPKADSTRLILHAKAQSTVMDLVRELGTVEDLELEDVMKVGYG
DVKCVESGGPEPGVGCAGRGVITAINFLEENGAYTPDLDFVFYDVLGDVVCGGFAMPIREGKAEEIYIVCSGEMMAMYAA
NNIAKGILKYASSGKVRLAGLICNARKTDKEYELIDALAKKLGTQMIHFVPRDNQVQRAELRRMTVIEYSPDHPQANEYR
SLAQKIADNKMLVVPTPLEMEELEDLLMEFGIMEAEDESIVGVAEAAAV

Sequences:

>Translated_289_residues
MRQIAIYGKGGIGKSTTTQNTVAGLASIGKKVMIVGCDPKADSTRLILHAKAQSTVMDLVRELGTVEDLELEDVMKVGYG
DVKCVESGGPEPGVGCAGRGVITAINFLEENGAYTPDLDFVFYDVLGDVVCGGFAMPIREGKAEEIYIVCSGEMMAMYAA
NNIAKGILKYASSGKVRLAGLICNARKTDKEYELIDALAKKLGTQMIHFVPRDNQVQRAELRRMTVIEYSPDHPQANEYR
SLAQKIADNKMLVVPTPLEMEELEDLLMEFGIMEAEDESIVGVAEAAAV
>Mature_289_residues
MRQIAIYGKGGIGKSTTTQNTVAGLASIGKKVMIVGCDPKADSTRLILHAKAQSTVMDLVRELGTVEDLELEDVMKVGYG
DVKCVESGGPEPGVGCAGRGVITAINFLEENGAYTPDLDFVFYDVLGDVVCGGFAMPIREGKAEEIYIVCSGEMMAMYAA
NNIAKGILKYASSGKVRLAGLICNARKTDKEYELIDALAKKLGTQMIHFVPRDNQVQRAELRRMTVIEYSPDHPQANEYR
SLAQKIADNKMLVVPTPLEMEELEDLLMEFGIMEAEDESIVGVAEAAAV

Specific function: The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components:the iron protein (component 2) and a component 1 which is either a molybdenum-iron protein, a vanadium-iron, or an iron-iron protein [H]

COG id: COG1348

COG function: function code P; Nitrogenase subunit NifH (ATPase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NifH/BchL/ChlL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000392
- InterPro:   IPR005977 [H]

Pfam domain/function: PF00142 Fer4_NifH [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 31293; Mature: 31293

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00746 NIFH_FRXC_1 ; PS00692 NIFH_FRXC_2 ; PS51026 NIFH_FRXC_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIAIYGKGGIGKSTTTQNTVAGLASIGKKVMIVGCDPKADSTRLILHAKAQSTVMDLV
CCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECHHHHHHHHH
RELGTVEDLELEDVMKVGYGDVKCVESGGPEPGVGCAGRGVITAINFLEENGAYTPDLDF
HHHCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
VFYDVLGDVVCGGFAMPIREGKAEEIYIVCSGEMMAMYAANNIAKGILKYASSGKVRLAG
HHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCCEEEEE
LICNARKTDKEYELIDALAKKLGTQMIHFVPRDNQVQRAELRRMTVIEYSPDHPQANEYR
EEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHEEEEEEECCCCCCHHHHH
SLAQKIADNKMLVVPTPLEMEELEDLLMEFGIMEAEDESIVGVAEAAAV
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHCC
>Mature Secondary Structure
MRQIAIYGKGGIGKSTTTQNTVAGLASIGKKVMIVGCDPKADSTRLILHAKAQSTVMDLV
CCEEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECHHHHHHHHH
RELGTVEDLELEDVMKVGYGDVKCVESGGPEPGVGCAGRGVITAINFLEENGAYTPDLDF
HHHCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHH
VFYDVLGDVVCGGFAMPIREGKAEEIYIVCSGEMMAMYAANNIAKGILKYASSGKVRLAG
HHHHHHHHHHHCCEECCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHHHHCCCCEEEEE
LICNARKTDKEYELIDALAKKLGTQMIHFVPRDNQVQRAELRRMTVIEYSPDHPQANEYR
EEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHEEEEEEECCCCCCHHHHH
SLAQKIADNKMLVVPTPLEMEELEDLLMEFGIMEAEDESIVGVAEAAAV
HHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2388847 [H]