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The map label for this gene is nifV [H]

Identifier: 197118455

GI number: 197118455

Start: 2395846

End: 2396994

Strand: Direct

Name: nifV [H]

Synonym: Gbem_2073

Alternate gene names: 197118455

Gene position: 2395846-2396994 (Clockwise)

Preceding gene: 197118454

Following gene: 197118456

Centisome position: 51.91

GC content: 63.71

Gene sequence:

>1149_bases
ATGGCTGGAGTTACCAAATCCCAGGTTTTCATCGGCGACACCACGCTGCGAGACGGCGAACAGACGGCAGGGGTGGTCTT
TACCGTAAAGGAAAAGATCTCCATCGCGAGGCAGTTGGACGCCATGGGCGTCCACGAACTGGAATGCGGCATACCCGCCA
TGGGCGAGGAGGAGCGCGACTCGATCCGGGCGCTGGTGGCGTTGGGCCTTTCGGCCCGGCTCATCACCTGGAACCGCGCG
GTGGTGCCGGACATCGAGGCGAGCATCGCCTGCGGCGTCGAAGCCGTGGACATCTCGCTCAGCGTCTCGGACATCATGAT
CGAACACAAGATCAACAAGAGCAGGGATTTTGTGAAGGAACAGCTGAAGCGGGCCCTATGCTTCGCCAAGCAGAACGGAC
TCTACGTCTGCGTCGGGGGCGAGGACGCCAGCCGCGCCGACAGCGATTTCCTGATCGAGTTGATGCAGATCGCCCAAACA
AACGGCGCCGATCGTTTCCGGTTCTGCGACACGCTCGGCATCCTCGACCCCTTCGCCATGTTCGAAAAAGTGGGTCGCCT
GAGAGCCGCGGTCCCCGGACTCGACATCGAGGTGCACACCCACAACGACCTGGGGCTCGCCACGGCGAACGCCCTGGCCG
GGGTGAGGGGAGGGGCGTCCTACATCAGCACCACGGTCAACGGCCTCGGCGAGCGGGCAGGCAACGCCGCATTGGAAGAG
GTGGTCATGGCGCTGAAGGTCGCCTGCGGCATTGACGTCGGCATCGACACCAGGCGCTTTCAGTCGGTGTCCCGGCTGGT
GGGGCGCGCCTCCAACCGCGAGGTCCCACCCTGGAAGGCTGTTGTGGGCGAGCGGGTCTTCTCCCACGAGTCGGGGTTGC
ATGCCGATGGCGTGCTGAAGGACCCGAAGAACTACGAGGGCTTCACGCCTGAGGAAGTGGGGCTGAAAAGGCACATCGTG
GCGGGAAAGCATTCCGGGACCAACGGGATCGTGGAAAGCTACCGTCAGATCGGCATTCCCATCTCCAGGGAAGAGGCGCA
GGAACTGATGGACAAGGTGAGGAGCACCGCGCAGCGCATCAAGGGGCCCCTGGCGCCCATGGACCTGGTCAAGCTGCACA
AGCGGAGAGGGGTTTCGCTGGCGGCCTAG

Upstream 100 bases:

>100_bases
GAACTTGGGCCGCTCGTGGCACAGCTTCTGCTAACCAGACTGCATCAGGGCAACGGCGCCCGGCACGCTTAACGGGGCGA
AAGCTCCGGAGGAGGGCGAG

Downstream 100 bases:

>100_bases
CGCTGAAGGCACCGGAGCCCGAGCTTCGAATCGATGCGTAAAAGTTAGGCAGAAAATCAGGCAGGTGGTTGGACGGCAAG
CCGGGAAGACAATTTTAAAA

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2 [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MAGVTKSQVFIGDTTLRDGEQTAGVVFTVKEKISIARQLDAMGVHELECGIPAMGEEERDSIRALVALGLSARLITWNRA
VVPDIEASIACGVEAVDISLSVSDIMIEHKINKSRDFVKEQLKRALCFAKQNGLYVCVGGEDASRADSDFLIELMQIAQT
NGADRFRFCDTLGILDPFAMFEKVGRLRAAVPGLDIEVHTHNDLGLATANALAGVRGGASYISTTVNGLGERAGNAALEE
VVMALKVACGIDVGIDTRRFQSVSRLVGRASNREVPPWKAVVGERVFSHESGLHADGVLKDPKNYEGFTPEEVGLKRHIV
AGKHSGTNGIVESYRQIGIPISREEAQELMDKVRSTAQRIKGPLAPMDLVKLHKRRGVSLAA

Sequences:

>Translated_382_residues
MAGVTKSQVFIGDTTLRDGEQTAGVVFTVKEKISIARQLDAMGVHELECGIPAMGEEERDSIRALVALGLSARLITWNRA
VVPDIEASIACGVEAVDISLSVSDIMIEHKINKSRDFVKEQLKRALCFAKQNGLYVCVGGEDASRADSDFLIELMQIAQT
NGADRFRFCDTLGILDPFAMFEKVGRLRAAVPGLDIEVHTHNDLGLATANALAGVRGGASYISTTVNGLGERAGNAALEE
VVMALKVACGIDVGIDTRRFQSVSRLVGRASNREVPPWKAVVGERVFSHESGLHADGVLKDPKNYEGFTPEEVGLKRHIV
AGKHSGTNGIVESYRQIGIPISREEAQELMDKVRSTAQRIKGPLAPMDLVKLHKRRGVSLAA
>Mature_381_residues
AGVTKSQVFIGDTTLRDGEQTAGVVFTVKEKISIARQLDAMGVHELECGIPAMGEEERDSIRALVALGLSARLITWNRAV
VPDIEASIACGVEAVDISLSVSDIMIEHKINKSRDFVKEQLKRALCFAKQNGLYVCVGGEDASRADSDFLIELMQIAQTN
GADRFRFCDTLGILDPFAMFEKVGRLRAAVPGLDIEVHTHNDLGLATANALAGVRGGASYISTTVNGLGERAGNAALEEV
VMALKVACGIDVGIDTRRFQSVSRLVGRASNREVPPWKAVVGERVFSHESGLHADGVLKDPKNYEGFTPEEVGLKRHIVA
GKHSGTNGIVESYRQIGIPISREEAQELMDKVRSTAQRIKGPLAPMDLVKLHKRRGVSLAA

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=377, Percent_Identity=35.0132625994695, Blast_Score=185, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320019, Length=348, Percent_Identity=30.7471264367816, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6320071, Length=348, Percent_Identity=30.7471264367816, Blast_Score=179, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6324682, Length=409, Percent_Identity=22.0048899755501, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582381, Length=299, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 41225; Mature: 41094

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGVTKSQVFIGDTTLRDGEQTAGVVFTVKEKISIARQLDAMGVHELECGIPAMGEEERD
CCCCCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHH
SIRALVALGLSARLITWNRAVVPDIEASIACGVEAVDISLSVSDIMIEHKINKSRDFVKE
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEEEEEEHHHHHHHHHCCCHHHHHHH
QLKRALCFAKQNGLYVCVGGEDASRADSDFLIELMQIAQTNGADRFRFCDTLGILDPFAM
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
FEKVGRLRAAVPGLDIEVHTHNDLGLATANALAGVRGGASYISTTVNGLGERAGNAALEE
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHH
VVMALKVACGIDVGIDTRRFQSVSRLVGRASNREVPPWKAVVGERVFSHESGLHADGVLK
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
DPKNYEGFTPEEVGLKRHIVAGKHSGTNGIVESYRQIGIPISREEAQELMDKVRSTAQRI
CCCCCCCCCHHHHCCHHHEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KGPLAPMDLVKLHKRRGVSLAA
CCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AGVTKSQVFIGDTTLRDGEQTAGVVFTVKEKISIARQLDAMGVHELECGIPAMGEEERD
CCCCCCEEEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHH
SIRALVALGLSARLITWNRAVVPDIEASIACGVEAVDISLSVSDIMIEHKINKSRDFVKE
HHHHHHHHCCCCEEEEECCCCCCCCHHHHHCCCEEEEEEEEHHHHHHHHHCCCHHHHHHH
QLKRALCFAKQNGLYVCVGGEDASRADSDFLIELMQIAQTNGADRFRFCDTLGILDPFAM
HHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHH
FEKVGRLRAAVPGLDIEVHTHNDLGLATANALAGVRGGASYISTTVNGLGERAGNAALEE
HHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHH
VVMALKVACGIDVGIDTRRFQSVSRLVGRASNREVPPWKAVVGERVFSHESGLHADGVLK
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
DPKNYEGFTPEEVGLKRHIVAGKHSGTNGIVESYRQIGIPISREEAQELMDKVRSTAQRI
CCCCCCCCCHHHHCCHHHEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
KGPLAPMDLVKLHKRRGVSLAA
CCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]