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The map label for this gene is afsQ1 [H]

Identifier: 197118407

GI number: 197118407

Start: 2331819

End: 2333177

Strand: Reverse

Name: afsQ1 [H]

Synonym: Gbem_2025

Alternate gene names: 197118407

Gene position: 2333177-2331819 (Counterclockwise)

Preceding gene: 197118408

Following gene: 197118406

Centisome position: 50.55

GC content: 61.3

Gene sequence:

>1359_bases
ATGCAGAAGAGCAACAACGGCCAGACGGCACCATACCTGATCCTGATCGTCGACGACAGCCCGACCCAGGCGAAACTCCT
TGAACAGCTCCTCGCCGGCGAGGGGTACCGCGTCGCGATCGCCAGAAACGGAAGCGACGCGCTGGAGCAGATCCGCGAGC
GGCAACCGGACCTGGTGATCAGCGACATACTGATGCCCGAGATGGACGGCTTCGAACTCTGCCGCCGAGTCAGGGGAATG
GAATCCATAAAGGACACCCCCATCGTCCTGCTCACCCACCTGAACGACCCCTCCGACGTCTTGCACAGCCTTGAGGCCGG
GGCCAACTACTTCGTGTCCAAGCCCTACAGCGACAAGCTGCTGCTCAACAGGATCCGAGACGTACTGCAGGGAGAGCGGG
CCTGTCGCATGGGGGAGAAAGAGGGAGAGGTCGCCATCAGCTACCACGGCAAGAACTACCGGGTACAGGCCGACAGCGCC
CACACCATCGAACTTTTGCTCTCGACCTACGAGCTCGCCGCCGAAAAGAACCAGGAGCTTCTGGGGGCCAAGGCGTCCCT
GTCCAACCTGAACCGCGAGCTGGAGCAGCGGGTGAAGGAGCGGACCGCGGCGCTCACCCAGGAGACGGCGGAGAGGCTGC
AGGCGATGGAAGAGCTGCGCAAAAAGGACGAGGTGCTGATGCAGCAAAGCCGCCAGGCCGCCATGGGGGAGATGATCGGC
AACATAGCCCACCAGTGGCGCCAGCCGCTGAACGCCGTTGGGCTGCTGGTGCAGGACCTGACGCTGAGCTACGAGTACGG
CAACTTCAACAAGGAGTACCTCGACTCCAGCACCAACAAGATCATGCAGATGGTGCGGCACATGTCGCAGACCATCGACG
ACTTCAGGAACTTCTTCATGCCCGACAAGGAGAAGACCAGCTTCGACCCCTGCCTGGTCGTGGAAAAAACCCTCAGCCTC
ATCGAGGGGAGCCTCGCCGACAAGGGGATAAGGTCCGAGGTCCACTGCGGCCACGCGCCGAACATAACCGGGCACCCCAA
CGAGTTCTCCCAGGTGCTCCTGAACATCCTGACCAACGCCATCGACGCCTTCGGGGAGAGGAAACCGGCCCAGCCTAAGC
TGCTGGTGAGCATAGATTACGAAGACGGCAAGATCGTGGTCACCATCGTCGACAACGCCGGAGGCATGCCGGAGCACGTC
CTGGGGCGGATCTTCGAGCCCTACTTCACCACCAAGGAGCAGGAGAAAGGAACCGGCATCGGCCTTTTCATGGCCAAGAA
CATCGTGGAAAAAAGCATGAACGGCTCGCTCACCGCAAGAAACATCGAGGGCGGGGCCGAATTCAGGATCGAGGTGTAG

Upstream 100 bases:

>100_bases
CGCCGTGCAGCACGTGCTCTCCCCCGAGGGGATCATCAACCTGCTTTTGGCGGTTACCAAGAAAGGCGCACAGGGTGCGT
CCAAGTAAGGAAGGAGCTTC

Downstream 100 bases:

>100_bases
CATGGGCGGATTAGCCATCGAGGACCCGGGCTTCACCCTTTTGTACGTGGAGGACGAGCAGGTCACCAGGGAGACGGTGC
TTTCCCTTTTGCAGCGGCGC

Product: response receiver histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MQKSNNGQTAPYLILIVDDSPTQAKLLEQLLAGEGYRVAIARNGSDALEQIRERQPDLVISDILMPEMDGFELCRRVRGM
ESIKDTPIVLLTHLNDPSDVLHSLEAGANYFVSKPYSDKLLLNRIRDVLQGERACRMGEKEGEVAISYHGKNYRVQADSA
HTIELLLSTYELAAEKNQELLGAKASLSNLNRELEQRVKERTAALTQETAERLQAMEELRKKDEVLMQQSRQAAMGEMIG
NIAHQWRQPLNAVGLLVQDLTLSYEYGNFNKEYLDSSTNKIMQMVRHMSQTIDDFRNFFMPDKEKTSFDPCLVVEKTLSL
IEGSLADKGIRSEVHCGHAPNITGHPNEFSQVLLNILTNAIDAFGERKPAQPKLLVSIDYEDGKIVVTIVDNAGGMPEHV
LGRIFEPYFTTKEQEKGTGIGLFMAKNIVEKSMNGSLTARNIEGGAEFRIEV

Sequences:

>Translated_452_residues
MQKSNNGQTAPYLILIVDDSPTQAKLLEQLLAGEGYRVAIARNGSDALEQIRERQPDLVISDILMPEMDGFELCRRVRGM
ESIKDTPIVLLTHLNDPSDVLHSLEAGANYFVSKPYSDKLLLNRIRDVLQGERACRMGEKEGEVAISYHGKNYRVQADSA
HTIELLLSTYELAAEKNQELLGAKASLSNLNRELEQRVKERTAALTQETAERLQAMEELRKKDEVLMQQSRQAAMGEMIG
NIAHQWRQPLNAVGLLVQDLTLSYEYGNFNKEYLDSSTNKIMQMVRHMSQTIDDFRNFFMPDKEKTSFDPCLVVEKTLSL
IEGSLADKGIRSEVHCGHAPNITGHPNEFSQVLLNILTNAIDAFGERKPAQPKLLVSIDYEDGKIVVTIVDNAGGMPEHV
LGRIFEPYFTTKEQEKGTGIGLFMAKNIVEKSMNGSLTARNIEGGAEFRIEV
>Mature_452_residues
MQKSNNGQTAPYLILIVDDSPTQAKLLEQLLAGEGYRVAIARNGSDALEQIRERQPDLVISDILMPEMDGFELCRRVRGM
ESIKDTPIVLLTHLNDPSDVLHSLEAGANYFVSKPYSDKLLLNRIRDVLQGERACRMGEKEGEVAISYHGKNYRVQADSA
HTIELLLSTYELAAEKNQELLGAKASLSNLNRELEQRVKERTAALTQETAERLQAMEELRKKDEVLMQQSRQAAMGEMIG
NIAHQWRQPLNAVGLLVQDLTLSYEYGNFNKEYLDSSTNKIMQMVRHMSQTIDDFRNFFMPDKEKTSFDPCLVVEKTLSL
IEGSLADKGIRSEVHCGHAPNITGHPNEFSQVLLNILTNAIDAFGERKPAQPKLLVSIDYEDGKIVVTIVDNAGGMPEHV
LGRIFEPYFTTKEQEKGTGIGLFMAKNIVEKSMNGSLTARNIEGGAEFRIEV

Specific function: Forms part of a two-component regulatory system AfsQ1/AfsQ2 involved in secondary metabolism [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=148, Percent_Identity=31.7567567567568, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788549, Length=238, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1790860, Length=164, Percent_Identity=28.6585365853659, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790437, Length=102, Percent_Identity=36.2745098039216, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788550, Length=108, Percent_Identity=36.1111111111111, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789809, Length=140, Percent_Identity=31.4285714285714, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786599, Length=117, Percent_Identity=29.0598290598291, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786911, Length=122, Percent_Identity=32.7868852459016, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=23.728813559322, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790299, Length=104, Percent_Identity=29.8076923076923, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788191, Length=116, Percent_Identity=32.7586206896552, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788905, Length=120, Percent_Identity=34.1666666666667, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87082012, Length=121, Percent_Identity=31.404958677686, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI145693157, Length=105, Percent_Identity=34.2857142857143, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787487, Length=116, Percent_Identity=32.7586206896552, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787229, Length=119, Percent_Identity=31.0924369747899, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1786784, Length=117, Percent_Identity=32.4786324786325, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790300, Length=301, Percent_Identity=23.2558139534884, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 50641; Mature: 50641

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKSNNGQTAPYLILIVDDSPTQAKLLEQLLAGEGYRVAIARNGSDALEQIRERQPDLVI
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCCEEE
SDILMPEMDGFELCRRVRGMESIKDTPIVLLTHLNDPSDVLHSLEAGANYFVSKPYSDKL
HHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCEEECCCCCHHH
LLNRIRDVLQGERACRMGEKEGEVAISYHGKNYRVQADSAHTIELLLSTYELAAEKNQEL
HHHHHHHHHCCHHHHHCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCHHH
LGAKASLSNLNRELEQRVKERTAALTQETAERLQAMEELRKKDEVLMQQSRQAAMGEMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIAHQWRQPLNAVGLLVQDLTLSYEYGNFNKEYLDSSTNKIMQMVRHMSQTIDDFRNFFM
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
PDKEKTSFDPCLVVEKTLSLIEGSLADKGIRSEVHCGHAPNITGHPNEFSQVLLNILTNA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IDAFGERKPAQPKLLVSIDYEDGKIVVTIVDNAGGMPEHVLGRIFEPYFTTKEQEKGTGI
HHHHCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
GLFMAKNIVEKSMNGSLTARNIEGGAEFRIEV
HHHHHHHHHHHHCCCCEEEEECCCCCCEEEEC
>Mature Secondary Structure
MQKSNNGQTAPYLILIVDDSPTQAKLLEQLLAGEGYRVAIARNGSDALEQIRERQPDLVI
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHCCCCEEE
SDILMPEMDGFELCRRVRGMESIKDTPIVLLTHLNDPSDVLHSLEAGANYFVSKPYSDKL
HHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCEEECCCCCHHH
LLNRIRDVLQGERACRMGEKEGEVAISYHGKNYRVQADSAHTIELLLSTYELAAEKNQEL
HHHHHHHHHCCHHHHHCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCHHH
LGAKASLSNLNRELEQRVKERTAALTQETAERLQAMEELRKKDEVLMQQSRQAAMGEMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIAHQWRQPLNAVGLLVQDLTLSYEYGNFNKEYLDSSTNKIMQMVRHMSQTIDDFRNFFM
HHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCC
PDKEKTSFDPCLVVEKTLSLIEGSLADKGIRSEVHCGHAPNITGHPNEFSQVLLNILTNA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IDAFGERKPAQPKLLVSIDYEDGKIVVTIVDNAGGMPEHVLGRIFEPYFTTKEQEKGTGI
HHHHCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCC
GLFMAKNIVEKSMNGSLTARNIEGGAEFRIEV
HHHHHHHHHHHHCCCCEEEEECCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339426; 12000953 [H]