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The map label for this gene is vhuA [H]

Identifier: 197118351

GI number: 197118351

Start: 2273030

End: 2274301

Strand: Reverse

Name: vhuA [H]

Synonym: Gbem_1968

Alternate gene names: 197118351

Gene position: 2274301-2273030 (Counterclockwise)

Preceding gene: 197118352

Following gene: 197118348

Centisome position: 49.28

GC content: 66.35

Gene sequence:

>1272_bases
ATGGGCAGCGTCGTTGAGTTGAACCTGACCCGGGTGGAGGGTCACGGCAGCGTCAAGGTCTACCGCGAGGGGTCGCGCGT
GGAGCGGGTGGAGCTATGTCTCACCGATTCTCCGCGGCTCTTCGAGGCGCTTCTGGTCGGCAAGAGCTATCTGGAAGTGC
CGGAGATCGTCTGCCGCATCTGCTCCCTCTGCTCGACGGTGCACAAGGTGACCGCGCTCCTGGCGGTGGAGAATGCCTTC
GGGGTGGAGGTCTCGGGGGCGACGGCCCTGACCCGCGAGCTCATCATGCAGGGGGGAATGATCCAGGACCACGCGCTGCA
TCTGTACTGCCTGCTCCTTCCCGACCTTCTCGGCGTGCCGGGAGTGACCGGGCTGGCCCAGAAGGCGCCCGAACTGCTGA
AGGCGGGACTTAGCATCAAGAGGGTCGGCAACATGATCCAGGAGACGGTCGGCGGTCGCCTCATCCACTCGGTCAACATC
CGGCTGGGAGGGCTGGGGCAGAGGGTGGGCAAAAAGGAACTGCTGCGCCTGCGCCATGAGCTTGAGTCGGTCCTTCCCGC
CTGCCGCGACGCCTACCGGCTCTTCCGCACTCCCTTCCCTTTCCCGGAACTCCCGTCCCCAAACGCATTGGCGGTGGACC
CATGCGGCGCCGGCCGTTCCGCCGCCATCCGGTGCCGCATGGGCGGAGGGGAATCCTTCGCCGTCTCCGGGTACCGCGAG
GCGATCAAAGAAAGCGTCCTTCCACACTCCAACGCCAAATATTCAAAGGTGATGGGGAAAGAAGCTACGGTGGGCGCGCT
CGCCAGGCTCTCCCTCGGCGTCCGGCTCAGAGCGCAGGCTCAGGGCGTGTTCGACGAGGTAAAGCACGAGATCCTCGGCA
GGGACATACGGGGCAACAGCCTGGCCCAGGCAATCGAGCTTTGCGACGCAGCCGAGCGCGCGATAGAGCTCATCGACCGC
CTCGTCGAGGAAGGCCAGGGTCCCCCCGACGACGTCGGACCGGTCCTGCGCGCGGGGAGAGGGAGTGCCGCCTGCGAGGC
GCCACGCGGCCTGCTGATCCACAGCTACGGCTTCGACTCGAACGGCATCTGCACCGAGGCCGACGTCGTCACCCCCACCG
CCTTGAACCAGGGGGCCATGGCGCGCGACCTGCTGGCGCTGGCGCGGGGAATGGAGGGGGAAGAGACGAAAAAGATGACC
ACGGCGCTGGAGCGCCTGATCAGGTGCTACGACCCCTGCATCTCGTGTGCGGTGCACGTGCTGAAGCTCTGA

Upstream 100 bases:

>100_bases
GAGGCGAACGTGGCGCAGGAGTTGGAGCTGTTGTTGGAGCGCGGGTTTGGCAGGAAGGAAATCGAGCGCAGGATGAGCCG
ATTCAAGCCGGAGTGGGACT

Downstream 100 bases:

>100_bases
CAGGGGCCAACGGTGCTACTTGCACAGCGTCTGCGACACTGGCATTGCATGCCGTCGCTGCCGCTTCACGAGATGAGGCT
GCAACGTTTATTGCCACCGC

Product: hydrogenase subunit alpha

Products: 2 Oxidized Ferredoxin; H(2). [C]

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MGSVVELNLTRVEGHGSVKVYREGSRVERVELCLTDSPRLFEALLVGKSYLEVPEIVCRICSLCSTVHKVTALLAVENAF
GVEVSGATALTRELIMQGGMIQDHALHLYCLLLPDLLGVPGVTGLAQKAPELLKAGLSIKRVGNMIQETVGGRLIHSVNI
RLGGLGQRVGKKELLRLRHELESVLPACRDAYRLFRTPFPFPELPSPNALAVDPCGAGRSAAIRCRMGGGESFAVSGYRE
AIKESVLPHSNAKYSKVMGKEATVGALARLSLGVRLRAQAQGVFDEVKHEILGRDIRGNSLAQAIELCDAAERAIELIDR
LVEEGQGPPDDVGPVLRAGRGSAACEAPRGLLIHSYGFDSNGICTEADVVTPTALNQGAMARDLLALARGMEGEETKKMT
TALERLIRCYDPCISCAVHVLKL

Sequences:

>Translated_423_residues
MGSVVELNLTRVEGHGSVKVYREGSRVERVELCLTDSPRLFEALLVGKSYLEVPEIVCRICSLCSTVHKVTALLAVENAF
GVEVSGATALTRELIMQGGMIQDHALHLYCLLLPDLLGVPGVTGLAQKAPELLKAGLSIKRVGNMIQETVGGRLIHSVNI
RLGGLGQRVGKKELLRLRHELESVLPACRDAYRLFRTPFPFPELPSPNALAVDPCGAGRSAAIRCRMGGGESFAVSGYRE
AIKESVLPHSNAKYSKVMGKEATVGALARLSLGVRLRAQAQGVFDEVKHEILGRDIRGNSLAQAIELCDAAERAIELIDR
LVEEGQGPPDDVGPVLRAGRGSAACEAPRGLLIHSYGFDSNGICTEADVVTPTALNQGAMARDLLALARGMEGEETKKMT
TALERLIRCYDPCISCAVHVLKL
>Mature_422_residues
GSVVELNLTRVEGHGSVKVYREGSRVERVELCLTDSPRLFEALLVGKSYLEVPEIVCRICSLCSTVHKVTALLAVENAFG
VEVSGATALTRELIMQGGMIQDHALHLYCLLLPDLLGVPGVTGLAQKAPELLKAGLSIKRVGNMIQETVGGRLIHSVNIR
LGGLGQRVGKKELLRLRHELESVLPACRDAYRLFRTPFPFPELPSPNALAVDPCGAGRSAAIRCRMGGGESFAVSGYREA
IKESVLPHSNAKYSKVMGKEATVGALARLSLGVRLRAQAQGVFDEVKHEILGRDIRGNSLAQAIELCDAAERAIELIDRL
VEEGQGPPDDVGPVLRAGRGSAACEAPRGLLIHSYGFDSNGICTEADVVTPTALNQGAMARDLLALARGMEGEETKKMTT
ALERLIRCYDPCISCAVHVLKL

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd1 Is Believed To Have A Role In Hydrogen Cycling During Fermentative Growth. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: 1.12.99.6 [C]

Molecular weight: Translated: 45429; Mature: 45298

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSVVELNLTRVEGHGSVKVYREGSRVERVELCLTDSPRLFEALLVGKSYLEVPEIVCRI
CCCEEEEEEEEECCCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
CSLCSTVHKVTALLAVENAFGVEVSGATALTRELIMQGGMIQDHALHLYCLLLPDLLGVP
HHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
GVTGLAQKAPELLKAGLSIKRVGNMIQETVGGRLIHSVNIRLGGLGQRVGKKELLRLRHE
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEEEEECCCHHHCCHHHHHHHHHH
LESVLPACRDAYRLFRTPFPFPELPSPNALAVDPCGAGRSAAIRCRMGGGESFAVSGYRE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCEEHHHHHH
AIKESVLPHSNAKYSKVMGKEATVGALARLSLGVRLRAQAQGVFDEVKHEILGRDIRGNS
HHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCCCCHH
LAQAIELCDAAERAIELIDRLVEEGQGPPDDVGPVLRAGRGSAACEAPRGLLIHSYGFDS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCC
NGICTEADVVTPTALNQGAMARDLLALARGMEGEETKKMTTALERLIRCYDPCISCAVHV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKL
HCC
>Mature Secondary Structure 
GSVVELNLTRVEGHGSVKVYREGSRVERVELCLTDSPRLFEALLVGKSYLEVPEIVCRI
CCEEEEEEEEECCCCCEEEEECCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
CSLCSTVHKVTALLAVENAFGVEVSGATALTRELIMQGGMIQDHALHLYCLLLPDLLGVP
HHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
GVTGLAQKAPELLKAGLSIKRVGNMIQETVGGRLIHSVNIRLGGLGQRVGKKELLRLRHE
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEEEEECCCHHHCCHHHHHHHHHH
LESVLPACRDAYRLFRTPFPFPELPSPNALAVDPCGAGRSAAIRCRMGGGESFAVSGYRE
HHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCCCEEHHHHHH
AIKESVLPHSNAKYSKVMGKEATVGALARLSLGVRLRAQAQGVFDEVKHEILGRDIRGNS
HHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHCCCCCCHH
LAQAIELCDAAERAIELIDRLVEEGQGPPDDVGPVLRAGRGSAACEAPRGLLIHSYGFDS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEEECCCCC
NGICTEADVVTPTALNQGAMARDLLALARGMEGEETKKMTTALERLIRCYDPCISCAVHV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Iron-Sulfur Cluster. [C]

Metal ions: Ni; Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 Reduced Ferredoxin; 2 H(+) [C]

Specific reaction: 2 Reduced Ferredoxin + 2 H(+) = 2 Oxidized Ferredoxin + H(2). [C]

General reaction: Redox reaction [C]

Inhibitor: Dimethyl sulfoxide; EDTA; NaCl; Tiron; Urea [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1603063 [H]