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The map label for this gene is fliY [H]

Identifier: 197118313

GI number: 197118313

Start: 2231534

End: 2232280

Strand: Direct

Name: fliY [H]

Synonym: Gbem_1930

Alternate gene names: 197118313

Gene position: 2231534-2232280 (Clockwise)

Preceding gene: 197118308

Following gene: 197118314

Centisome position: 48.35

GC content: 57.43

Gene sequence:

>747_bases
ATGAAAGCTATTCGTATCATGGCAGTCGTTGTGGCATTCGCAGGTCTTTGTTTTGCCTCTGCAGCCCTGGCCGCACCGAA
AACGGTAACCGTCGCTACCGACGCGACCTGGCCCCCGATGGAGTTTGTGGATGCCAACAAGAAGATCGTCGGTTTCGATA
TCGACTTCATGAACGCAGTCGCCAAGGAGGCGGGTTTCCAGGCCTCCTTCAAGAACACTGCCTGGGACGGCATCTTCGCC
GGCATCGCCGCCGGCCAGTACGACGCCATCATCTCCTCGGTCACCATCACCGACGAGCGCAAGGCGAAGTTCGACTTCAC
TGTTCCTTACATCAGCATCGGCCAGATCCTGGTGGTGCCCAAAGCCGAGAAGGGGACGAAGATCGGCGATCTGAAGGGCA
AGAAAATCGGCGCCCAGATCGGCACTACCGGTGCCATGGAGATCAAGAAGGTCGCCGGCGTGGAGCTGAAGACCTATGAC
GAAGTAGGTCTCGCTTTCGAGGACATGGCAGCCGGGCGCATCTCCGGTGTCGTCTGCGACCAGCCGACCGCCGTCACCTA
CGCGCTGGACAAGAAGGAGTACAGCAACAAGTTCAAGATCGTGGGCCAGCCCTTCACCAAAGAGGCTTACGGCATCGTGG
TCAAGAAGGGTAACAAGGAGCTGGTCGACCAGCTGAACAAGGGAATCGCGGCAGTGCAGAAGAAAAAGATCGACCAGCAG
ATCAAGAAGAAGTGGCACCTTAAGTAG

Upstream 100 bases:

>100_bases
TTATTAAGTGGTTGACAAGAGACGATTTTATCTTTAAAAGTATGCCCGAAAATGTTAAAGGTGTGTTCTGTTTAATATTA
AAGGGAACCAAGGAGGAAGT

Downstream 100 bases:

>100_bases
TGCACGATTGGCTCCTGCAGGAGCGGCACGCAATGGCGCTGACCCCGCCGAAGCCCCCTGCAGGAGCTGTTTTTTGGCCC
AAGCGCGTATACGGGAAAAG

Product: polar amino acid/opine ABC transporter periplasmic amino acid-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKAIRIMAVVVAFAGLCFASAALAAPKTVTVATDATWPPMEFVDANKKIVGFDIDFMNAVAKEAGFQASFKNTAWDGIFA
GIAAGQYDAIISSVTITDERKAKFDFTVPYISIGQILVVPKAEKGTKIGDLKGKKIGAQIGTTGAMEIKKVAGVELKTYD
EVGLAFEDMAAGRISGVVCDQPTAVTYALDKKEYSNKFKIVGQPFTKEAYGIVVKKGNKELVDQLNKGIAAVQKKKIDQQ
IKKKWHLK

Sequences:

>Translated_248_residues
MKAIRIMAVVVAFAGLCFASAALAAPKTVTVATDATWPPMEFVDANKKIVGFDIDFMNAVAKEAGFQASFKNTAWDGIFA
GIAAGQYDAIISSVTITDERKAKFDFTVPYISIGQILVVPKAEKGTKIGDLKGKKIGAQIGTTGAMEIKKVAGVELKTYD
EVGLAFEDMAAGRISGVVCDQPTAVTYALDKKEYSNKFKIVGQPFTKEAYGIVVKKGNKELVDQLNKGIAAVQKKKIDQQ
IKKKWHLK
>Mature_248_residues
MKAIRIMAVVVAFAGLCFASAALAAPKTVTVATDATWPPMEFVDANKKIVGFDIDFMNAVAKEAGFQASFKNTAWDGIFA
GIAAGQYDAIISSVTITDERKAKFDFTVPYISIGQILVVPKAEKGTKIGDLKGKKIGAQIGTTGAMEIKKVAGVELKTYD
EVGLAFEDMAAGRISGVVCDQPTAVTYALDKKEYSNKFKIVGQPFTKEAYGIVVKKGNKELVDQLNKGIAAVQKKKIDQQ
IKKKWHLK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=221, Percent_Identity=34.841628959276, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1787088, Length=254, Percent_Identity=33.8582677165354, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787085, Length=247, Percent_Identity=31.5789473684211, Blast_Score=121, Evalue=4e-29,
Organism=Escherichia coli, GI1787031, Length=243, Percent_Identity=30.4526748971193, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788649, Length=257, Percent_Identity=29.1828793774319, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=27.0742358078603, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26732; Mature: 26732

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIRIMAVVVAFAGLCFASAALAAPKTVTVATDATWPPMEFVDANKKIVGFDIDFMNAV
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCEEEEECHHHHHHH
AKEAGFQASFKNTAWDGIFAGIAAGQYDAIISSVTITDERKAKFDFTVPYISIGQILVVP
HHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHEEEEECCCCCCEEEEECCEEEECCEEEEE
KAEKGTKIGDLKGKKIGAQIGTTGAMEIKKVAGVELKTYDEVGLAFEDMAAGRISGVVCD
CCCCCCEECCCCCCEECCCCCCCCCHHHHHHCCCEEEEHHHHCCCHHHHCCCHHCEEEEC
QPTAVTYALDKKEYSNKFKIVGQPFTKEAYGIVVKKGNKELVDQLNKGIAAVQKKKIDQQ
CCCEEEEEECCHHHCCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
IKKKWHLK
HHHHCCCC
>Mature Secondary Structure
MKAIRIMAVVVAFAGLCFASAALAAPKTVTVATDATWPPMEFVDANKKIVGFDIDFMNAV
CCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHCCCCEEEEECHHHHHHH
AKEAGFQASFKNTAWDGIFAGIAAGQYDAIISSVTITDERKAKFDFTVPYISIGQILVVP
HHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHEEEEECCCCCCEEEEECCEEEECCEEEEE
KAEKGTKIGDLKGKKIGAQIGTTGAMEIKKVAGVELKTYDEVGLAFEDMAAGRISGVVCD
CCCCCCEECCCCCCEECCCCCCCCCHHHHHHCCCEEEEHHHHCCCHHHHCCCHHCEEEEC
QPTAVTYALDKKEYSNKFKIVGQPFTKEAYGIVVKKGNKELVDQLNKGIAAVQKKKIDQQ
CCCEEEEEECCHHHCCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
IKKKWHLK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]