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The map label for this gene is pyrB

Identifier: 197118279

GI number: 197118279

Start: 2195128

End: 2196060

Strand: Direct

Name: pyrB

Synonym: Gbem_1896

Alternate gene names: 197118279

Gene position: 2195128-2196060 (Clockwise)

Preceding gene: 197118278

Following gene: 197118280

Centisome position: 47.56

GC content: 62.59

Gene sequence:

>933_bases
ATGGGTTTCAGGCATAAAGACATCATCGCCCTGAAGGATCTGACCAAGGAGGAGATCACGCTCCTTCTGGACACGGCTGA
CAGTCTCAGCGAGATCAACCAGCGGGACATCAAAAAGGTGCCGACGCTGCGCGGCAAAACGGTGATCAACCTCTTTTACG
AGGCATCCACCAGGACCCGCACTTCGTTCGAGATTGCCGCCAAAAGGCTTTCCGCCGATGCGGTGAACATCACCGCGTCC
ACCAGTTCCGTGGTCAAGGGCGAGACACTGTCCGACACCGCCAACAACCTCCTCGCCATGAAGCCCGACATCATCGTGAT
GCGCCACGCCGTTTCCGGCGCCCATGAATACCTAGCCAAGCGGGTCTCCTGCTCGGTCATCAACGCCGGCGACGGCGCCC
ACGAGCACCCTTCCCAGGGTCTTTTGGACATGCTCACCATGCGCCAGAAGTTCGGCAAGCTGGACGGGCTGAAGGTAGCC
ATCATCGGCGACATCACCCACAGCCGCGTGGCCCGCTCCGACATCTACGGCCTCACCACCATGGGCTCCCACGTATTCCT
CGCCGGTCCGCCGACCATGATGCCGGTCGGCATCGAGCGGCTTGGCAACGTCACGGTCTGCAAGGACATGCGGGAGGCCG
TGGACAAGGCCGATGTGGTGATGATGCTGAGGATCCAGTTGGAGCGCCAGGGCAAGACCCTGCTCCCCAGCATGCGCGAG
TACTCGCGCTACTTCGGGCTCAACCCCGAGGTGCTCGGCCTGGCCAAGAAGAACGCCATCGTCATGCATCCGGGTCCCAT
CAACCGCGGCGTCGAGCTCGCCTCCTCGGTTGCCGACTGCGACCAGTCCGCCATCCTGACCCAGGTGGAAAACGGCGTGG
CGGTGCGCATGGCGATGCTGTACCACGTCTGCGGCGGCGAGCCGGTGGAGTAA

Upstream 100 bases:

>100_bases
ATAAAAACTTCCTCCTCTACGGGGGGAAAGCCATAAATTGATATCGTTTTGCTTTAACGCTGGAGCGCGCCGGTGCGCTC
CGCAAACCGAGGGGGGGACC

Downstream 100 bases:

>100_bases
ACCAAGCTGACGTTCAACGTTCAACGTTTGGCAGAACCGGATGTTGAATTGGCGCCTCACGAGTGAAGTAGCCGTACCAC
GTTTGACGCCATAGGCAGTG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MGFRHKDIIALKDLTKEEITLLLDTADSLSEINQRDIKKVPTLRGKTVINLFYEASTRTRTSFEIAAKRLSADAVNITAS
TSSVVKGETLSDTANNLLAMKPDIIVMRHAVSGAHEYLAKRVSCSVINAGDGAHEHPSQGLLDMLTMRQKFGKLDGLKVA
IIGDITHSRVARSDIYGLTTMGSHVFLAGPPTMMPVGIERLGNVTVCKDMREAVDKADVVMMLRIQLERQGKTLLPSMRE
YSRYFGLNPEVLGLAKKNAIVMHPGPINRGVELASSVADCDQSAILTQVENGVAVRMAMLYHVCGGEPVE

Sequences:

>Translated_310_residues
MGFRHKDIIALKDLTKEEITLLLDTADSLSEINQRDIKKVPTLRGKTVINLFYEASTRTRTSFEIAAKRLSADAVNITAS
TSSVVKGETLSDTANNLLAMKPDIIVMRHAVSGAHEYLAKRVSCSVINAGDGAHEHPSQGLLDMLTMRQKFGKLDGLKVA
IIGDITHSRVARSDIYGLTTMGSHVFLAGPPTMMPVGIERLGNVTVCKDMREAVDKADVVMMLRIQLERQGKTLLPSMRE
YSRYFGLNPEVLGLAKKNAIVMHPGPINRGVELASSVADCDQSAILTQVENGVAVRMAMLYHVCGGEPVE
>Mature_309_residues
GFRHKDIIALKDLTKEEITLLLDTADSLSEINQRDIKKVPTLRGKTVINLFYEASTRTRTSFEIAAKRLSADAVNITAST
SSVVKGETLSDTANNLLAMKPDIIVMRHAVSGAHEYLAKRVSCSVINAGDGAHEHPSQGLLDMLTMRQKFGKLDGLKVAI
IGDITHSRVARSDIYGLTTMGSHVFLAGPPTMMPVGIERLGNVTVCKDMREAVDKADVVMMLRIQLERQGKTLLPSMREY
SRYFGLNPEVLGLAKKNAIVMHPGPINRGVELASSVADCDQSAILTQVENGVAVRMAMLYHVCGGEPVE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=35.5987055016181, Blast_Score=181, Evalue=9e-46,
Organism=Homo sapiens, GI38788445, Length=177, Percent_Identity=32.7683615819209, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI2367364, Length=310, Percent_Identity=35.8064516129032, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790703, Length=231, Percent_Identity=29.8701298701299, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786469, Length=227, Percent_Identity=28.1938325991189, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=322, Percent_Identity=33.5403726708075, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=32.5878594249201, Blast_Score=146, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24642586, Length=310, Percent_Identity=32.9032258064516, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_GEOBB (B5EB49)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138706.1
- GeneID:   6781886
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1896
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33787; Mature: 33656

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFRHKDIIALKDLTKEEITLLLDTADSLSEINQRDIKKVPTLRGKTVINLFYEASTRTR
CCCCCCCCHHHHHCCHHHEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
TSFEIAAKRLSADAVNITASTSSVVKGETLSDTANNLLAMKPDIIVMRHAVSGAHEYLAK
HHHHHHHHHHCCCEEEEEECCCHHCCCCCHHHHHCCEEEECCCEEEEEHHHHHHHHHHHH
RVSCSVINAGDGAHEHPSQGLLDMLTMRQKFGKLDGLKVAIIGDITHSRVARSDIYGLTT
HHHEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCEEEE
MGSHVFLAGPPTMMPVGIERLGNVTVCKDMREAVDKADVVMMLRIQLERQGKTLLPSMRE
CCCEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEHHHHCCCCCCHHHHH
YSRYFGLNPEVLGLAKKNAIVMHPGPINRGVELASSVADCDQSAILTQVENGVAVRMAML
HHHHHCCCCHHEEEECCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHH
YHVCGGEPVE
HHHCCCCCCC
>Mature Secondary Structure 
GFRHKDIIALKDLTKEEITLLLDTADSLSEINQRDIKKVPTLRGKTVINLFYEASTRTR
CCCCCCCHHHHHCCHHHEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
TSFEIAAKRLSADAVNITASTSSVVKGETLSDTANNLLAMKPDIIVMRHAVSGAHEYLAK
HHHHHHHHHHCCCEEEEEECCCHHCCCCCHHHHHCCEEEECCCEEEEEHHHHHHHHHHHH
RVSCSVINAGDGAHEHPSQGLLDMLTMRQKFGKLDGLKVAIIGDITHSRVARSDIYGLTT
HHHEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHCEEEE
MGSHVFLAGPPTMMPVGIERLGNVTVCKDMREAVDKADVVMMLRIQLERQGKTLLPSMRE
CCCEEEEECCCCCCCHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEHHHHCCCCCCHHHHH
YSRYFGLNPEVLGLAKKNAIVMHPGPINRGVELASSVADCDQSAILTQVENGVAVRMAML
HHHHHCCCCHHEEEECCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHH
YHVCGGEPVE
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA