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The map label for this gene is hldE

Identifier: 197117256

GI number: 197117256

Start: 1019676

End: 1021139

Strand: Direct

Name: hldE

Synonym: Gbem_0864

Alternate gene names: 197117256

Gene position: 1019676-1021139 (Clockwise)

Preceding gene: 308535199

Following gene: 197117257

Centisome position: 22.09

GC content: 65.51

Gene sequence:

>1464_bases
GTGGAACGTAGGGAAGTAGAATCCTTTTTCGAGCGGGCCACTTCGATCCGGGCCCTCGTTGTCGGCGACCTGATGCTGGA
CGAGTACCTCTGGGGACGGGCGGAACGGATCTCGCCGGAGGCCCCGGTGCAGGTGGTGGAGGTGGCCAGAGAAGACCTCC
GTCTCGGCGGCGCCGGCAACGTGGTGAATAACCTCGCCGCCCTGGGATGCCAGGTCAGCGTCTGCTCGGTGATTGGTAGC
GACGAGAACGGAGCGCTCCTGCGCCGCGCCCTGGAGGAGAAAGGGGCGGGACTGGAAGGGCTTTTCGAGGAAGGCGAGAG
AAGGACCAGCAAGAAGACGCGCGTGCTCGCGGCGAACCAGCAGATCGTCAGGATCGACCGGGAGACCAAGTCGTCCATAG
CAGCCGCCAGCGAGCAGGGGATTATCGACTACCTTGCCGCCCGAAGCGGCGACTTCGACGTGATCGTGGTCTCGGATTAC
CTGAAAGGGGTCCTGACCCCGGCGGTCCTTTCGGCGGTCTGCCGCAGCGGCAGGGAACTGGGGATCCCGGTGGTGGTCGA
CCCCAAGGGAAACGACTACGGCAAGTACCGCGGCGCCACCATACTGACCCCGAACCGCAAGGAGGCGGAAATTGCCTCGG
GGGTAGCCATCACGGACCAGGAATCGCTGCAGTGCGCAGCGAGCGGGCTTTTGTCCTGCCTGGAGCTGGACGCGCTTCTC
ATCACGCGCAGCGAGGCCGGGATGTCGCTCTTCCCGGCGGCCGGGGGGGCGGTTCATATCCCGACCGTGGCCCGCGAGGT
GTTCGACGTGACCGGCGCCGGGGACACCGTGATCTCGGTCCTTTCGCTGGGGCTTGCCTGCGGCCTCACCATGGCTGACG
CAGCCTGGATCGCCAACGTGGCGGCGGGGATCGCGGTCGGCAAACTCGGTACCTCGACGGTCGCCCCGCAGGAGATCGTC
GCGGAGGTGGGGCACGGTGCTAAGGACAGCGACTCCAAGATCAAGAACCTGGACGTCCTGGCGCACGCCATAGCCCGCGA
GCACTCCCGCGGCAAGAAGGTGGTCTTTACCAACGGCTGCTTCGACCTTCTGCACGTCGGGCACGTGAAGTACCTGCAAA
AGGCGAGGGGGCTCGGGGACATCCTGGTGGTGGGGCTAAATACAGACGCCTCGGTCCGCCGCCTCAAGGGGGAGAAGCGC
CCCCTCATCGAGGAGACGGAGCGGGCCCACATCCTGGCGGCGTTGGACTGCATCGACTACGTGGTGCTCTTCGACGAGGA
CACCCCCTTGAAGGTAATCGAGACGCTTGGGCCGGACATCCTGGTGAAAGGCGGCGACTACAGCATCGAAGGGGTGGTCG
GGCGCGAGGTGGTGGAGGCCCGCGGGGGACGCGTGGAGTTGATCCAGTTCGTCGACGGCAGGTCCACGAGCCGCATCATC
GAGAAGATCCTTTCCTCGTATTGA

Upstream 100 bases:

>100_bases
TTTTCAGGACGAAGTCAGATAGTAAGACAGATATGACCTTTAGCCTTAGCCGTCTGTCGAACATTGAACGTTGAACCTTG
AACGGGTTTCTGGAGGTTTT

Downstream 100 bases:

>100_bases
GAGTGGGGGGAGGGGGATAGAAGCGATGGATAAAGACGCGAAAATCTACGTCGCCGGCCACCGCGGCCTGGTCGGCTCCG
CCCTGGTGCGGGAGCTCGCT

Product: bifunctional D-glycero-D-mannoheptose-7-phosphate kinase/D-glycero-D-mannoheptose-1-phosphate adenylyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MERREVESFFERATSIRALVVGDLMLDEYLWGRAERISPEAPVQVVEVAREDLRLGGAGNVVNNLAALGCQVSVCSVIGS
DENGALLRRALEEKGAGLEGLFEEGERRTSKKTRVLAANQQIVRIDRETKSSIAAASEQGIIDYLAARSGDFDVIVVSDY
LKGVLTPAVLSAVCRSGRELGIPVVVDPKGNDYGKYRGATILTPNRKEAEIASGVAITDQESLQCAASGLLSCLELDALL
ITRSEAGMSLFPAAGGAVHIPTVAREVFDVTGAGDTVISVLSLGLACGLTMADAAWIANVAAGIAVGKLGTSTVAPQEIV
AEVGHGAKDSDSKIKNLDVLAHAIAREHSRGKKVVFTNGCFDLLHVGHVKYLQKARGLGDILVVGLNTDASVRRLKGEKR
PLIEETERAHILAALDCIDYVVLFDEDTPLKVIETLGPDILVKGGDYSIEGVVGREVVEARGGRVELIQFVDGRSTSRII
EKILSSY

Sequences:

>Translated_487_residues
MERREVESFFERATSIRALVVGDLMLDEYLWGRAERISPEAPVQVVEVAREDLRLGGAGNVVNNLAALGCQVSVCSVIGS
DENGALLRRALEEKGAGLEGLFEEGERRTSKKTRVLAANQQIVRIDRETKSSIAAASEQGIIDYLAARSGDFDVIVVSDY
LKGVLTPAVLSAVCRSGRELGIPVVVDPKGNDYGKYRGATILTPNRKEAEIASGVAITDQESLQCAASGLLSCLELDALL
ITRSEAGMSLFPAAGGAVHIPTVAREVFDVTGAGDTVISVLSLGLACGLTMADAAWIANVAAGIAVGKLGTSTVAPQEIV
AEVGHGAKDSDSKIKNLDVLAHAIAREHSRGKKVVFTNGCFDLLHVGHVKYLQKARGLGDILVVGLNTDASVRRLKGEKR
PLIEETERAHILAALDCIDYVVLFDEDTPLKVIETLGPDILVKGGDYSIEGVVGREVVEARGGRVELIQFVDGRSTSRII
EKILSSY
>Mature_487_residues
MERREVESFFERATSIRALVVGDLMLDEYLWGRAERISPEAPVQVVEVAREDLRLGGAGNVVNNLAALGCQVSVCSVIGS
DENGALLRRALEEKGAGLEGLFEEGERRTSKKTRVLAANQQIVRIDRETKSSIAAASEQGIIDYLAARSGDFDVIVVSDY
LKGVLTPAVLSAVCRSGRELGIPVVVDPKGNDYGKYRGATILTPNRKEAEIASGVAITDQESLQCAASGLLSCLELDALL
ITRSEAGMSLFPAAGGAVHIPTVAREVFDVTGAGDTVISVLSLGLACGLTMADAAWIANVAAGIAVGKLGTSTVAPQEIV
AEVGHGAKDSDSKIKNLDVLAHAIAREHSRGKKVVFTNGCFDLLHVGHVKYLQKARGLGDILVVGLNTDASVRRLKGEKR
PLIEETERAHILAALDCIDYVVLFDEDTPLKVIETLGPDILVKGGDYSIEGVVGREVVEARGGRVELIQFVDGRSTSRII
EKILSSY

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Homo sapiens, GI4505651, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI296841136, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789432, Length=474, Percent_Identity=44.5147679324894, Blast_Score=366, Evalue=1e-102,
Organism=Drosophila melanogaster, GI19921220, Length=143, Percent_Identity=34.965034965035, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45550973, Length=143, Percent_Identity=34.965034965035, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550974, Length=143, Percent_Identity=34.965034965035, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45550975, Length=143, Percent_Identity=34.965034965035, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_GEOBB (B5EEZ1)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137683.1
- ProteinModelPortal:   B5EEZ1
- SMR:   B5EEZ1
- GeneID:   6783702
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0864
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   CLSK828725
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 51837; Mature: 51837

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERREVESFFERATSIRALVVGDLMLDEYLWGRAERISPEAPVQVVEVAREDLRLGGAGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCCCCH
VVNNLAALGCQVSVCSVIGSDENGALLRRALEEKGAGLEGLFEEGERRTSKKTRVLAANQ
HHHHHHHHCCCEEEHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCC
QIVRIDRETKSSIAAASEQGIIDYLAARSGDFDVIVVSDYLKGVLTPAVLSAVCRSGREL
EEEEECCHHHHHHHHHHHCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCC
GIPVVVDPKGNDYGKYRGATILTPNRKEAEIASGVAITDQESLQCAASGLLSCLELDALL
CCEEEECCCCCCCCCCCCEEEECCCCCHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHE
ITRSEAGMSLFPAAGGAVHIPTVAREVFDVTGAGDTVISVLSLGLACGLTMADAAWIANV
EEECCCCCEEECCCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
AAGIAVGKLGTSTVAPQEIVAEVGHGAKDSDSKIKNLDVLAHAIAREHSRGKKVVFTNGC
HHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
FDLLHVGHVKYLQKARGLGDILVVGLNTDASVRRLKGEKRPLIEETERAHILAALDCIDY
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCE
VVLFDEDTPLKVIETLGPDILVKGGDYSIEGVVGREVVEARGGRVELIQFVDGRSTSRII
EEEECCCCCHHHHHHCCCCEEEECCCEEECCHHHHHHHHHCCCEEEEEEEECCCCHHHHH
EKILSSY
HHHHHCC
>Mature Secondary Structure
MERREVESFFERATSIRALVVGDLMLDEYLWGRAERISPEAPVQVVEVAREDLRLGGAGN
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHCCCCCCH
VVNNLAALGCQVSVCSVIGSDENGALLRRALEEKGAGLEGLFEEGERRTSKKTRVLAANQ
HHHHHHHHCCCEEEHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEECCC
QIVRIDRETKSSIAAASEQGIIDYLAARSGDFDVIVVSDYLKGVLTPAVLSAVCRSGREL
EEEEECCHHHHHHHHHHHCCHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCC
GIPVVVDPKGNDYGKYRGATILTPNRKEAEIASGVAITDQESLQCAASGLLSCLELDALL
CCEEEECCCCCCCCCCCCEEEECCCCCHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHE
ITRSEAGMSLFPAAGGAVHIPTVAREVFDVTGAGDTVISVLSLGLACGLTMADAAWIANV
EEECCCCCEEECCCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHH
AAGIAVGKLGTSTVAPQEIVAEVGHGAKDSDSKIKNLDVLAHAIAREHSRGKKVVFTNGC
HHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCC
FDLLHVGHVKYLQKARGLGDILVVGLNTDASVRRLKGEKRPLIEETERAHILAALDCIDY
HHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCE
VVLFDEDTPLKVIETLGPDILVKGGDYSIEGVVGREVVEARGGRVELIQFVDGRSTSRII
EEEECCCCCHHHHHHCCCCEEEECCCEEECCHHHHHHHHHCCCEEEEEEEECCCCHHHHH
EKILSSY
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA