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The map label for this gene is fcl-1 [H]

Identifier: 197117257

GI number: 197117257

Start: 1021165

End: 1022094

Strand: Direct

Name: fcl-1 [H]

Synonym: Gbem_0865

Alternate gene names: 197117257

Gene position: 1021165-1022094 (Clockwise)

Preceding gene: 197117256

Following gene: 308535200

Centisome position: 22.13

GC content: 62.8

Gene sequence:

>930_bases
ATGGATAAAGACGCGAAAATCTACGTCGCCGGCCACCGCGGCCTGGTCGGCTCCGCCCTGGTGCGGGAGCTCGCTCGCCT
TGGCTACCGAAACCTGTTGCTGCGCGAAAGCCGTGAGCTTGACCTGCGCAACCAGGCGGATACGCTGGCCTTCTTCCAGG
CGGAGCGTCCGGAATACGTTTTTCTCGCCGCGGCCAAGGTGGGCGGGATCGCGGCCAACAACAGCTTCCCGGCCGACTTC
ATCTACGACAATCTGATGATCCAGAACAACGTGATCCACTCCGCCTACCTGAACGGGGTCATCAAACTTTTGCTTTTGGG
CTCCACCTGCATCTATCCGAGGCTCGCGCCGCAGCCGATCCGGGAGGAGGCGCTTCTGACCGGTCCGCTGGAGCCGACTA
ACGAGCCTTACGCCATCGCCAAGATAGCGGGAATCAAGCTGTGCCAGTCCTACAACCGCCAGTACGGCACCCGCTTCATC
AGCGCCATGCCGACGAACCTCTACGGCCCCAACGACAACTTCGACCTCGACTCCTCGCACGTGCTCCCGGCGCTGATGAG
GAAGTTCCACGAGGCGAAGGTCTCCGGCAGCCAGAGCGTCACCGTCTGGGGGAGCGGCACCCCTTACCGCGAGTTCGTCC
ACGTGGACGACGTGGCGCGGGCATCGCTCTTTCTCATGGAGCGCTACGAGGGATGGGAACCGGTGAACATAGGGAGCGGG
CAGGAGCTGACCATACGGGAGCTGGCCGAAAAGATCCGGGAGGTGGTCGGTTTCACCGGGGAGATCGTCTTCGACAGCTC
CAAACCGGACGGCACTCCCCGAAAACTCAGCGACGTAAGCCGCATCCACCAGCTTGGCTGGAGGCACGGCATCGAGCTTG
TGCAGGGGCTCCGTGACACCTACGCCTGGTACCTCGGCAACCTCTGCTGA

Upstream 100 bases:

>100_bases
CGCGTGGAGTTGATCCAGTTCGTCGACGGCAGGTCCACGAGCCGCATCATCGAGAAGATCCTTTCCTCGTATTGAGAGTG
GGGGGAGGGGGATAGAAGCG

Downstream 100 bases:

>100_bases
CCGGCTGCAACCACTGTTTTGTACCCTTTTTCAAACTTTGTGCCAAAAGTAACGGTTTGGCCAAAATTGGCTTTACAGTG
AAATTTTATGATGCTATACT

Product: GDP-L-fucose synthase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MDKDAKIYVAGHRGLVGSALVRELARLGYRNLLLRESRELDLRNQADTLAFFQAERPEYVFLAAAKVGGIAANNSFPADF
IYDNLMIQNNVIHSAYLNGVIKLLLLGSTCIYPRLAPQPIREEALLTGPLEPTNEPYAIAKIAGIKLCQSYNRQYGTRFI
SAMPTNLYGPNDNFDLDSSHVLPALMRKFHEAKVSGSQSVTVWGSGTPYREFVHVDDVARASLFLMERYEGWEPVNIGSG
QELTIRELAEKIREVVGFTGEIVFDSSKPDGTPRKLSDVSRIHQLGWRHGIELVQGLRDTYAWYLGNLC

Sequences:

>Translated_309_residues
MDKDAKIYVAGHRGLVGSALVRELARLGYRNLLLRESRELDLRNQADTLAFFQAERPEYVFLAAAKVGGIAANNSFPADF
IYDNLMIQNNVIHSAYLNGVIKLLLLGSTCIYPRLAPQPIREEALLTGPLEPTNEPYAIAKIAGIKLCQSYNRQYGTRFI
SAMPTNLYGPNDNFDLDSSHVLPALMRKFHEAKVSGSQSVTVWGSGTPYREFVHVDDVARASLFLMERYEGWEPVNIGSG
QELTIRELAEKIREVVGFTGEIVFDSSKPDGTPRKLSDVSRIHQLGWRHGIELVQGLRDTYAWYLGNLC
>Mature_309_residues
MDKDAKIYVAGHRGLVGSALVRELARLGYRNLLLRESRELDLRNQADTLAFFQAERPEYVFLAAAKVGGIAANNSFPADF
IYDNLMIQNNVIHSAYLNGVIKLLLLGSTCIYPRLAPQPIREEALLTGPLEPTNEPYAIAKIAGIKLCQSYNRQYGTRFI
SAMPTNLYGPNDNFDLDSSHVLPALMRKFHEAKVSGSQSVTVWGSGTPYREFVHVDDVARASLFLMERYEGWEPVNIGSG
QELTIRELAEKIREVVGFTGEIVFDSSKPDGTPRKLSDVSRIHQLGWRHGIELVQGLRDTYAWYLGNLC

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=308, Percent_Identity=36.038961038961, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI7657641, Length=237, Percent_Identity=24.4725738396624, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI42516563, Length=325, Percent_Identity=25.2307692307692, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI1788365, Length=311, Percent_Identity=59.4855305466238, Blast_Score=384, Evalue=1e-108,
Organism=Escherichia coli, GI48994969, Length=345, Percent_Identity=24.0579710144928, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554428, Length=307, Percent_Identity=31.9218241042345, Blast_Score=169, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17539532, Length=319, Percent_Identity=25.705329153605, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115532424, Length=271, Percent_Identity=27.3062730627306, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922778, Length=314, Percent_Identity=32.1656050955414, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21356223, Length=321, Percent_Identity=24.6105919003115, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 34579; Mature: 34579

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKDAKIYVAGHRGLVGSALVRELARLGYRNLLLRESRELDLRNQADTLAFFQAERPEYV
CCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHEEECCCCCEE
FLAAAKVGGIAANNSFPADFIYDNLMIQNNVIHSAYLNGVIKLLLLGSTCIYPRLAPQPI
EEEEEHHCCEECCCCCCHHHHHHCEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCC
REEALLTGPLEPTNEPYAIAKIAGIKLCQSYNRQYGTRFISAMPTNLYGPNDNFDLDSSH
CCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
VLPALMRKFHEAKVSGSQSVTVWGSGTPYREFVHVDDVARASLFLMERYEGWEPVNIGSG
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHEEHHHHHHHHHHHHHHHCCCCCCCCCCC
QELTIRELAEKIREVVGFTGEIVFDSSKPDGTPRKLSDVSRIHQLGWRHGIELVQGLRDT
CEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAWYLGNLC
HHHHHCCCC
>Mature Secondary Structure
MDKDAKIYVAGHRGLVGSALVRELARLGYRNLLLRESRELDLRNQADTLAFFQAERPEYV
CCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCHHHHHEEECCCCCEE
FLAAAKVGGIAANNSFPADFIYDNLMIQNNVIHSAYLNGVIKLLLLGSTCIYPRLAPQPI
EEEEEHHCCEECCCCCCHHHHHHCEEEECCHHHHHHHHHHHHHHHHCCHHCCCCCCCCCC
REEALLTGPLEPTNEPYAIAKIAGIKLCQSYNRQYGTRFISAMPTNLYGPNDNFDLDSSH
CCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
VLPALMRKFHEAKVSGSQSVTVWGSGTPYREFVHVDDVARASLFLMERYEGWEPVNIGSG
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHEEHHHHHHHHHHHHHHHCCCCCCCCCCC
QELTIRELAEKIREVVGFTGEIVFDSSKPDGTPRKLSDVSRIHQLGWRHGIELVQGLRDT
CEEHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAWYLGNLC
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]