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The map label for this gene is yagA [H]

Identifier: 197116739

GI number: 197116739

Start: 414470

End: 415606

Strand: Reverse

Name: yagA [H]

Synonym: Gbem_0339

Alternate gene names: 197116739

Gene position: 415606-414470 (Counterclockwise)

Preceding gene: 197116740

Following gene: 197116732

Centisome position: 9.01

GC content: 51.36

Gene sequence:

>1137_bases
ATGCCTTGGCGACCGGTGGATACTATGACCCTGAGATTCGAGTTCGTTCATTTGGCGTTGCAGGAAGGCAGCAACATAGC
CTTCCTTTGCAAACGCTTTAACATCAGCCGTCCGACCGGCTACAAATGGATTAGCAGGTTCCTTGAAACCGGCGTCGAAG
GCCTAATTGACTTATCTCGCAAGCCACTGACCTGTCCACATAAGACGGTCACTGCGACCGAAGATGCTATTCTCAAGCTT
CGCGAAGAACATCAGGCTTGGGGAGCACGTAAGCTACGTAAGCGTCTTGAAAAACTTGGGCATCAGGGGGTGCCTGTACC
TTCGACCATAACAGCAATCTTGCGCCGCAACGGCTGCATTGATCCAGCTGAGTCAGATAAACATACTGCTTTCCAACGGT
TCGAGCACCCATACCCAAACGACCTATGGCAGATGGATTTCAAGGGCAGTATTCCGATGAAGCATCATGGTCGCTGTCAT
CCGTTGACTGTTCTGGATGACCATTCCCGGTTTAGTGTGGTGCTTGTAGCCTGTGCCAATGAGCGAACAGAAACGGTTAA
AGCTGCTTTGATTGAGGCATTCAGGCAATATGGATTGCCATATCGAATGACCATGGACAATGGCAGCCCATGGGGTAACG
ATCAATACAACGACCTTACGCCATTAACGGTCTGGCTTATCAGAAACGAGATACGGGTCAGCCACTCTCGCCCCTATCAT
CCTCAAACACAGGGTAAGGACGAGCGGTTTCACAGAACTCTGGTTGCAGAAGCAATCGCTGGAAGAGTCTTCGAAGACCT
AGCTGACTGCCAGCTTAGATTCGACACCTTCAGGCATTGCTACAACTTGGAGCGCCCACACGAATCACTAGGTATGGAAA
CTCCGATGACGCGTTATAAGCCTAGCCGGCGGACTTACTCAGAAATACTGCCCGAGATTCAGTACGCCCCAGACGACGAG
ATCCGAAAGGTCCAGGCCGAGGGTAAGGTGTCATTCAAAGGCCGATGTGTGCGCCTCGCGAAGGCGCTCAGGGGGCAGCC
GGTGGCATTTCGGCCAACCATACGCGATGGCGAATATGGCGTTTATTTCTGCAGCCAGAAAATCGCAGAAGTCGATCTCA
AAGAACAAAAAGTGTAA

Upstream 100 bases:

>100_bases
GCCCCCTCCCGCTGTATAGACTGGGGACATGGTTTACAAATGTTCGGGGACATAGTTAACACTTTTGCCAAGAATGACCT
TTCCTCGGAGGAGGGAGACC

Downstream 100 bases:

>100_bases
ACCATGTCCCCGAACAACTGTAAACCTTGACCCCGGTCCATACACCCGCAAGGGGAGGGGGGACTAAGAGCGAGGCTCCC
CGAAACTCTCCGCCAAACCT

Product: transposase of ISGbem_A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPWRPVDTMTLRFEFVHLALQEGSNIAFLCKRFNISRPTGYKWISRFLETGVEGLIDLSRKPLTCPHKTVTATEDAILKL
REEHQAWGARKLRKRLEKLGHQGVPVPSTITAILRRNGCIDPAESDKHTAFQRFEHPYPNDLWQMDFKGSIPMKHHGRCH
PLTVLDDHSRFSVVLVACANERTETVKAALIEAFRQYGLPYRMTMDNGSPWGNDQYNDLTPLTVWLIRNEIRVSHSRPYH
PQTQGKDERFHRTLVAEAIAGRVFEDLADCQLRFDTFRHCYNLERPHESLGMETPMTRYKPSRRTYSEILPEIQYAPDDE
IRKVQAEGKVSFKGRCVRLAKALRGQPVAFRPTIRDGEYGVYFCSQKIAEVDLKEQKV

Sequences:

>Translated_378_residues
MPWRPVDTMTLRFEFVHLALQEGSNIAFLCKRFNISRPTGYKWISRFLETGVEGLIDLSRKPLTCPHKTVTATEDAILKL
REEHQAWGARKLRKRLEKLGHQGVPVPSTITAILRRNGCIDPAESDKHTAFQRFEHPYPNDLWQMDFKGSIPMKHHGRCH
PLTVLDDHSRFSVVLVACANERTETVKAALIEAFRQYGLPYRMTMDNGSPWGNDQYNDLTPLTVWLIRNEIRVSHSRPYH
PQTQGKDERFHRTLVAEAIAGRVFEDLADCQLRFDTFRHCYNLERPHESLGMETPMTRYKPSRRTYSEILPEIQYAPDDE
IRKVQAEGKVSFKGRCVRLAKALRGQPVAFRPTIRDGEYGVYFCSQKIAEVDLKEQKV
>Mature_377_residues
PWRPVDTMTLRFEFVHLALQEGSNIAFLCKRFNISRPTGYKWISRFLETGVEGLIDLSRKPLTCPHKTVTATEDAILKLR
EEHQAWGARKLRKRLEKLGHQGVPVPSTITAILRRNGCIDPAESDKHTAFQRFEHPYPNDLWQMDFKGSIPMKHHGRCHP
LTVLDDHSRFSVVLVACANERTETVKAALIEAFRQYGLPYRMTMDNGSPWGNDQYNDLTPLTVWLIRNEIRVSHSRPYHP
QTQGKDERFHRTLVAEAIAGRVFEDLADCQLRFDTFRHCYNLERPHESLGMETPMTRYKPSRRTYSEILPEIQYAPDDEI
RKVQAEGKVSFKGRCVRLAKALRGQPVAFRPTIRDGEYGVYFCSQKIAEVDLKEQKV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786462, Length=378, Percent_Identity=48.6772486772487, Blast_Score=356, Evalue=1e-99,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 43777; Mature: 43645

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWRPVDTMTLRFEFVHLALQEGSNIAFLCKRFNISRPTGYKWISRFLETGVEGLIDLSR
CCCCCCCHHEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
KPLTCPHKTVTATEDAILKLREEHQAWGARKLRKRLEKLGHQGVPVPSTITAILRRNGCI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
DPAESDKHTAFQRFEHPYPNDLWQMDFKGSIPMKHHGRCHPLTVLDDHSRFSVVLVACAN
CCCCCCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECC
ERTETVKAALIEAFRQYGLPYRMTMDNGSPWGNDQYNDLTPLTVWLIRNEIRVSHSRPYH
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCC
PQTQGKDERFHRTLVAEAIAGRVFEDLADCQLRFDTFRHCYNLERPHESLGMETPMTRYK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHCC
PSRRTYSEILPEIQYAPDDEIRKVQAEGKVSFKGRCVRLAKALRGQPVAFRPTIRDGEYG
CCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCE
VYFCSQKIAEVDLKEQKV
EEECCCHHHHCCHHHCCC
>Mature Secondary Structure 
PWRPVDTMTLRFEFVHLALQEGSNIAFLCKRFNISRPTGYKWISRFLETGVEGLIDLSR
CCCCCCHHEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCC
KPLTCPHKTVTATEDAILKLREEHQAWGARKLRKRLEKLGHQGVPVPSTITAILRRNGCI
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCC
DPAESDKHTAFQRFEHPYPNDLWQMDFKGSIPMKHHGRCHPLTVLDDHSRFSVVLVACAN
CCCCCCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEEECCCCCEEEEEEEECC
ERTETVKAALIEAFRQYGLPYRMTMDNGSPWGNDQYNDLTPLTVWLIRNEIRVSHSRPYH
CHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCC
PQTQGKDERFHRTLVAEAIAGRVFEDLADCQLRFDTFRHCYNLERPHESLGMETPMTRYK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHCC
PSRRTYSEILPEIQYAPDDEIRKVQAEGKVSFKGRCVRLAKALRGQPVAFRPTIRDGEYG
CCCHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCE
VYFCSQKIAEVDLKEQKV
EEECCCHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8113168 [H]