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The map label for this gene is rsmE [H]

Identifier: 197116640

GI number: 197116640

Start: 283882

End: 284622

Strand: Reverse

Name: rsmE [H]

Synonym: Gbem_0240

Alternate gene names: 197116640

Gene position: 284622-283882 (Counterclockwise)

Preceding gene: 197116641

Following gene: 197116629

Centisome position: 6.17

GC content: 66.53

Gene sequence:

>741_bases
ATGCGCAGTTTCTTTCTCGGCGACAACGCCATCTGCGGCGACAGCGTGACCATCACCGGAGAGCTTTACCGCCACATGGC
GCGCGTGCTGCGCATTAAACAAGGGAGCGAGGTGGAGCTCATCGAGGACGGCGACTCCCGTCACCGGGGAACCGTCGAGG
AGGTGGGGGCGAAAAGCCTCACGGTGCGCATCAGCGCCAGCGCCGCTCCTGCCGCCGAGGAACCGGGGCTGCGCATCACG
CTCCTGCAGGGGATGCCCAAGGGGGAGAAGCTCGACCTGATCCTGCAGAAATGCACCGAGCTCGGGGTCGCCGAAGTGGT
CGCCTTTGACGCGGCACGCTCAGTGGTGAAGCTGCGCGGGGATCGAAGCGCCACCCGGCTCGCCCGTTACGAAAAGATAG
TGCGGGAGGCCGCCCGGCAGTCCGGGCGGCGTTCTGCGCCGAAGGTGGCCCTGGGAGGGACCCTCGCCGATGTCCTGGCT
GATTCACACCATGAGGTAAAGCTGCTTCTTTACGAGGGGGAAGAGAAGACGACCCTGCACGGCTGCTTCGGCGCTTTCGA
GGCACCGGAGAGCGTGGCGGTCGTGGTGGGACCGGAAGGGGGATTGGCCCCCGAGGAGGTGCGGCAGGCGCTGGCGGCAG
GGTTCACCCCGGTCACACTGGGAAAGAGGATCCTGCGCACCGAGACCGCCGGGCTCGCCATGCTGTCCATCCTGCAGTTT
CACTGGGGGGACATGGGGTAG

Upstream 100 bases:

>100_bases
AGAAGCCTTCGTCTGCGCGGCGTTCAATTCGCTGGAGCTGGTGGAAAACCCGAAGGAGCTCGAGTGGTCCTGCCTCAGCT
TCAGGAAACCCCTGTAGACC

Downstream 100 bases:

>100_bases
CCAGGCTTCAGCGCAGCGCCACCACCAGCGCCGCGATGGCGGTCAAAAGCGACACAGCCGTCGCGGTCATCATCATTTTC
TGCCTGGCGACCACAACCTT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRSFFLGDNAICGDSVTITGELYRHMARVLRIKQGSEVELIEDGDSRHRGTVEEVGAKSLTVRISASAAPAAEEPGLRIT
LLQGMPKGEKLDLILQKCTELGVAEVVAFDAARSVVKLRGDRSATRLARYEKIVREAARQSGRRSAPKVALGGTLADVLA
DSHHEVKLLLYEGEEKTTLHGCFGAFEAPESVAVVVGPEGGLAPEEVRQALAAGFTPVTLGKRILRTETAGLAMLSILQF
HWGDMG

Sequences:

>Translated_246_residues
MRSFFLGDNAICGDSVTITGELYRHMARVLRIKQGSEVELIEDGDSRHRGTVEEVGAKSLTVRISASAAPAAEEPGLRIT
LLQGMPKGEKLDLILQKCTELGVAEVVAFDAARSVVKLRGDRSATRLARYEKIVREAARQSGRRSAPKVALGGTLADVLA
DSHHEVKLLLYEGEEKTTLHGCFGAFEAPESVAVVVGPEGGLAPEEVRQALAAGFTPVTLGKRILRTETAGLAMLSILQF
HWGDMG
>Mature_246_residues
MRSFFLGDNAICGDSVTITGELYRHMARVLRIKQGSEVELIEDGDSRHRGTVEEVGAKSLTVRISASAAPAAEEPGLRIT
LLQGMPKGEKLDLILQKCTELGVAEVVAFDAARSVVKLRGDRSATRLARYEKIVREAARQSGRRSAPKVALGGTLADVLA
DSHHEVKLLLYEGEEKTTLHGCFGAFEAPESVAVVVGPEGGLAPEEVRQALAAGFTPVTLGKRILRTETAGLAMLSILQF
HWGDMG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=238, Percent_Identity=30.672268907563, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26388; Mature: 26388

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSFFLGDNAICGDSVTITGELYRHMARVLRIKQGSEVELIEDGDSRHRGTVEEVGAKSL
CCCEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCEE
TVRISASAAPAAEEPGLRITLLQGMPKGEKLDLILQKCTELGVAEVVAFDAARSVVKLRG
EEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
DRSATRLARYEKIVREAARQSGRRSAPKVALGGTLADVLADSHHEVKLLLYEGEEKTTLH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHCCCCCCEEEEEEECCCCCCHH
GCFGAFEAPESVAVVVGPEGGLAPEEVRQALAAGFTPVTLGKRILRTETAGLAMLSILQF
HHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HWGDMG
HCCCCC
>Mature Secondary Structure
MRSFFLGDNAICGDSVTITGELYRHMARVLRIKQGSEVELIEDGDSRHRGTVEEVGAKSL
CCCEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCEE
TVRISASAAPAAEEPGLRITLLQGMPKGEKLDLILQKCTELGVAEVVAFDAARSVVKLRG
EEEEECCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
DRSATRLARYEKIVREAARQSGRRSAPKVALGGTLADVLADSHHEVKLLLYEGEEKTTLH
CCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHCCCCCCEEEEEEECCCCCCHH
GCFGAFEAPESVAVVVGPEGGLAPEEVRQALAAGFTPVTLGKRILRTETAGLAMLSILQF
HHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
HWGDMG
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]