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The map label for this gene is fixJ [H]

Identifier: 197106265

GI number: 197106265

Start: 3216692

End: 3217297

Strand: Direct

Name: fixJ [H]

Synonym: PHZ_c2804

Alternate gene names: 197106265

Gene position: 3216692-3217297 (Clockwise)

Preceding gene: 197106264

Following gene: 197106266

Centisome position: 80.49

GC content: 69.14

Gene sequence:

>606_bases
TTGAGTAGCGGTCTGGTGCATGTCATCGACGACGACGAGGCGATGCGCCAGTCGTTGCAGTTCCTGCTGGACACGGCGGG
CTTCCAGGCGCGGACCTATGAGTCCGCGGTGCAGTTCCTGGAGGGGATCGAGATACTGGAGCCGGGCTGCATCGTCACCG
ACGTGCGGATGCCCGAGATGAACGGCCTGGAGCTGGTGCGCCGGCTGCGGACCCGGGGCGTGACCCAGCCGGTCATCGTC
ATCACCGGCCACGGCGACGTGCCGCTGGCGGTCGAGGCCATGAAGGCCGGCGTCGCCGACTTCCTGGAGAAGCCGTTCGA
CGACGAGGCGCTGCTGTCCGCGGTGCGCGCGGCCCTCGACACCCGGGTGCAGGACGACCGCCAGGACGCCGAGCGGCAGA
AGTTCGAGTCCATGCTGGCGGGCCTGTCGCCGCGCGAGCAGGACGTGCTGCGCGGGGTCGTGGCCGGCAAGCCCAACAAG
ATCATCGCCTTCGAGCTGGGCATCAGCCCGCGGACGGTCGAGGTCTACCGGGCCAACGTGATGACCAAGACGGGGGCGTC
CAGCCTCTCCGAACTGGTCCGGATGGCGCTGCTCGCGGGGTTCTAG

Upstream 100 bases:

>100_bases
GAGGCCCACGGCGGGCGCATCTGGGTTGAGCCGAACCCCAAGGGCGGTGCTATCTTCCGCTTCACCCTTCGGGCGGTGGC
GGATGAGGAGTTGACCGGCA

Downstream 100 bases:

>100_bases
CGGCGCCCGCCCGCCGTTGAGTTGCGTCAAAGCAGCCTTGCGCGGCGCCGGGCATTCTGCCTGCATGCCGCCGAGCCGCC
CACCCCTGCCCCCGCTCATC

Product: response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSSGLVHVIDDDEAMRQSLQFLLDTAGFQARTYESAVQFLEGIEILEPGCIVTDVRMPEMNGLELVRRLRTRGVTQPVIV
ITGHGDVPLAVEAMKAGVADFLEKPFDDEALLSAVRAALDTRVQDDRQDAERQKFESMLAGLSPREQDVLRGVVAGKPNK
IIAFELGISPRTVEVYRANVMTKTGASSLSELVRMALLAGF

Sequences:

>Translated_201_residues
MSSGLVHVIDDDEAMRQSLQFLLDTAGFQARTYESAVQFLEGIEILEPGCIVTDVRMPEMNGLELVRRLRTRGVTQPVIV
ITGHGDVPLAVEAMKAGVADFLEKPFDDEALLSAVRAALDTRVQDDRQDAERQKFESMLAGLSPREQDVLRGVVAGKPNK
IIAFELGISPRTVEVYRANVMTKTGASSLSELVRMALLAGF
>Mature_200_residues
SSGLVHVIDDDEAMRQSLQFLLDTAGFQARTYESAVQFLEGIEILEPGCIVTDVRMPEMNGLELVRRLRTRGVTQPVIVI
TGHGDVPLAVEAMKAGVADFLEKPFDDEALLSAVRAALDTRVQDDRQDAERQKFESMLAGLSPREQDVLRGVVAGKPNKI
IAFELGISPRTVEVYRANVMTKTGASSLSELVRMALLAGF

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=152, Percent_Identity=35.5263157894737, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI1788222, Length=209, Percent_Identity=28.2296650717703, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788550, Length=112, Percent_Identity=31.25, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790102, Length=206, Percent_Identity=32.0388349514563, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788905, Length=122, Percent_Identity=34.4262295081967, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788712, Length=170, Percent_Identity=28.2352941176471, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI1790437, Length=153, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=1e-12,
Organism=Escherichia coli, GI1788521, Length=195, Percent_Identity=31.7948717948718, Blast_Score=64, Evalue=5e-12,
Organism=Escherichia coli, GI1789809, Length=114, Percent_Identity=31.5789473684211, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1787375, Length=114, Percent_Identity=28.9473684210526, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1787473, Length=181, Percent_Identity=30.3867403314917, Blast_Score=60, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22003; Mature: 21871

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGLVHVIDDDEAMRQSLQFLLDTAGFQARTYESAVQFLEGIEILEPGCIVTDVRMPEM
CCCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
NGLELVRRLRTRGVTQPVIVITGHGDVPLAVEAMKAGVADFLEKPFDDEALLSAVRAALD
CHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TRVQDDRQDAERQKFESMLAGLSPREQDVLRGVVAGKPNKIIAFELGISPRTVEVYRANV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEHHHHHHH
MTKTGASSLSELVRMALLAGF
HHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSGLVHVIDDDEAMRQSLQFLLDTAGFQARTYESAVQFLEGIEILEPGCIVTDVRMPEM
CCCEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCC
NGLELVRRLRTRGVTQPVIVITGHGDVPLAVEAMKAGVADFLEKPFDDEALLSAVRAALD
CHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TRVQDDRQDAERQKFESMLAGLSPREQDVLRGVVAGKPNKIIAFELGISPRTVEVYRANV
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEHHHHHHH
MTKTGASSLSELVRMALLAGF
HHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]