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The map label for this gene is fixL [H]

Identifier: 197106264

GI number: 197106264

Start: 3215203

End: 3216699

Strand: Direct

Name: fixL [H]

Synonym: PHZ_c2803

Alternate gene names: 197106264

Gene position: 3215203-3216699 (Clockwise)

Preceding gene: 197106263

Following gene: 197106265

Centisome position: 80.46

GC content: 71.41

Gene sequence:

>1497_bases
ATGGGGCCTCCGGACAAGCTATCTCCACGCGCGTGGTGGCGCGGCCGCGTCGCCGGCTTCGGCGTCGCGATCGGCGCGAC
CGCGGCCTGCCTGATGCTGCGGCTGGCCCTGGAGCCGGTGATCCACGACCGGGCGCTGTTCCTGCTGTTCGTGCCGGCCC
TGATGGCCGCCGCGGCGGTGGGCGGCTTCGCCCCGGCCCTGGCGGCCACCGTGGTCAGCCTGGCGATCCACGCGGCGCTG
ATGGGCGACAGCCTGGTCTCCGACACGGCCAACCTGATCAGCGCGGTGGTGTTCGCCCTGCTCGGCCTGCTGAGCGGCCT
GGCCGGCTCGCACCTGCGCAAGATGGTCGCCGAGACCGAGCACGCCCTGGAGGACCTGGAGACCCGCGAGGCGCACCTGC
GCTCGATCCTCGACACCGTGCCGGACGCCATGATCGTGATCGACGAGCACGGGGTCATGCAGTCGTTCAGCGCCGCGGCC
GAGCGGCTGTTCGGCTGGACCGCCGCCGAGTCCATCGGCCGCAACGTCAGCGTGCTGATGCCCAACCCCTACCGCGACGC
CCACGACGGCTACCTGCACCGCTACCTCTCCACCGGCGAGCGGCGGATCATCGGCATCGGCCGGGTGGTGGTGGGCGAGC
GCAAGGACGGCTCGACCTTCCCGATGGAGCTGTCGGTGGGCGAGATGCGGGTGGGCGAGCGGCGCTTCTTCACCGGCTTC
GTCCGCGACCTGACCGAACGCCAGGCCACCGAGCGGCGGCTGCAGGACCTGCAGTCCGAGCTGGTCCACGTGGCGCGCCT
GACCGCCCTGGGCGAGATGGCCTCGGCCCTGGCCCACGAGCTGAACCAGCCGCTCTCGGCGGCGGCGAACTACGTCAAGG
GCTCAGTCCGGCTGCTGGACCAGGAGCCGCTCGACAAGCCCAAGATCAAGGACGCCCTGAACCTGGCCGGCGAGCAGACC
CTGCGGGCGGGCCAGATCATCCGCCGCCTGCGCGACTTCGTCGCCAAGGGCGAGGCCGAGCGCAGCCTCGAGAGCCTGCC
CCAGCTGCTGGAGGAGGCCGGCGCCCTGGCCATGGTCGGCGCCAAGGAGCGCGGCGTGCGCCTGAGCTTCCAGATCAGCC
CCGAGGTGGGCCTGGTGCTGGCCGACAAGGTGCAGATCCAGCAGGTCGTGCTGAACCTGATGCGCAACGCCATCGACGCC
ATGGAGGGCTCGGCCCGGCGCGAGCTGGTGGTCAGCGCCCGTCCGGTCGAGGACGAGATGGTCGAGATCTCGGTGGCCGA
CACCGGCCCGGGGGTCAGCCCCGAGGTGGCCGACCACCTGTTCCAGCCCTTCATCACCACCAAGCGGGAGGGGATGGGCG
TGGGGCTGTCGATCAGCCGCACGATCGTCGAGGCCCACGGCGGGCGCATCTGGGTTGAGCCGAACCCCAAGGGCGGTGCT
ATCTTCCGCTTCACCCTTCGGGCGGTGGCGGATGAGGAGTTGACCGGCATTGAGTAG

Upstream 100 bases:

>100_bases
AGCGCCCAACGGAGCGTCCCGCCGGCGGGACGGGATGAGCCCCGCCCCCGACGCAGGCGAAAGGTTGCCTCCGGCCGCCT
TCCCGCCTACGCCTGGGGCC

Downstream 100 bases:

>100_bases
CGGTCTGGTGCATGTCATCGACGACGACGAGGCGATGCGCCAGTCGTTGCAGTTCCTGCTGGACACGGCGGGCTTCCAGG
CGCGGACCTATGAGTCCGCG

Product: sensory box histidine kinase FixL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MGPPDKLSPRAWWRGRVAGFGVAIGATAACLMLRLALEPVIHDRALFLLFVPALMAAAAVGGFAPALAATVVSLAIHAAL
MGDSLVSDTANLISAVVFALLGLLSGLAGSHLRKMVAETEHALEDLETREAHLRSILDTVPDAMIVIDEHGVMQSFSAAA
ERLFGWTAAESIGRNVSVLMPNPYRDAHDGYLHRYLSTGERRIIGIGRVVVGERKDGSTFPMELSVGEMRVGERRFFTGF
VRDLTERQATERRLQDLQSELVHVARLTALGEMASALAHELNQPLSAAANYVKGSVRLLDQEPLDKPKIKDALNLAGEQT
LRAGQIIRRLRDFVAKGEAERSLESLPQLLEEAGALAMVGAKERGVRLSFQISPEVGLVLADKVQIQQVVLNLMRNAIDA
MEGSARRELVVSARPVEDEMVEISVADTGPGVSPEVADHLFQPFITTKREGMGVGLSISRTIVEAHGGRIWVEPNPKGGA
IFRFTLRAVADEELTGIE

Sequences:

>Translated_498_residues
MGPPDKLSPRAWWRGRVAGFGVAIGATAACLMLRLALEPVIHDRALFLLFVPALMAAAAVGGFAPALAATVVSLAIHAAL
MGDSLVSDTANLISAVVFALLGLLSGLAGSHLRKMVAETEHALEDLETREAHLRSILDTVPDAMIVIDEHGVMQSFSAAA
ERLFGWTAAESIGRNVSVLMPNPYRDAHDGYLHRYLSTGERRIIGIGRVVVGERKDGSTFPMELSVGEMRVGERRFFTGF
VRDLTERQATERRLQDLQSELVHVARLTALGEMASALAHELNQPLSAAANYVKGSVRLLDQEPLDKPKIKDALNLAGEQT
LRAGQIIRRLRDFVAKGEAERSLESLPQLLEEAGALAMVGAKERGVRLSFQISPEVGLVLADKVQIQQVVLNLMRNAIDA
MEGSARRELVVSARPVEDEMVEISVADTGPGVSPEVADHLFQPFITTKREGMGVGLSISRTIVEAHGGRIWVEPNPKGGA
IFRFTLRAVADEELTGIE
>Mature_497_residues
GPPDKLSPRAWWRGRVAGFGVAIGATAACLMLRLALEPVIHDRALFLLFVPALMAAAAVGGFAPALAATVVSLAIHAALM
GDSLVSDTANLISAVVFALLGLLSGLAGSHLRKMVAETEHALEDLETREAHLRSILDTVPDAMIVIDEHGVMQSFSAAAE
RLFGWTAAESIGRNVSVLMPNPYRDAHDGYLHRYLSTGERRIIGIGRVVVGERKDGSTFPMELSVGEMRVGERRFFTGFV
RDLTERQATERRLQDLQSELVHVARLTALGEMASALAHELNQPLSAAANYVKGSVRLLDQEPLDKPKIKDALNLAGEQTL
RAGQIIRRLRDFVAKGEAERSLESLPQLLEEAGALAMVGAKERGVRLSFQISPEVGLVLADKVQIQQVVLNLMRNAIDAM
EGSARRELVVSARPVEDEMVEISVADTGPGVSPEVADHLFQPFITTKREGMGVGLSISRTIVEAHGGRIWVEPNPKGGAI
FRFTLRAVADEELTGIE

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=29.6918767507003, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=30.0847457627119, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1790300, Length=345, Percent_Identity=24.9275362318841, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786912, Length=515, Percent_Identity=24.8543689320388, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI48994928, Length=406, Percent_Identity=25.3694581280788, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789149, Length=227, Percent_Identity=29.9559471365639, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788713, Length=433, Percent_Identity=22.1709006928406, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788393, Length=137, Percent_Identity=32.8467153284672, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI87081816, Length=226, Percent_Identity=29.2035398230088, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87081921, Length=122, Percent_Identity=27.8688524590164, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53825; Mature: 53694

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPPDKLSPRAWWRGRVAGFGVAIGATAACLMLRLALEPVIHDRALFLLFVPALMAAAAV
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFAPALAATVVSLAIHAALMGDSLVSDTANLISAVVFALLGLLSGLAGSHLRKMVAETE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HALEDLETREAHLRSILDTVPDAMIVIDEHGVMQSFSAAAERLFGWTAAESIGRNVSVLM
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PNPYRDAHDGYLHRYLSTGERRIIGIGRVVVGERKDGSTFPMELSVGEMRVGERRFFTGF
CCCCCCCHHHHHHHHHHCCCCEEEEECEEEEECCCCCCCCCEEEECCCHHCCHHHHHHHH
VRDLTERQATERRLQDLQSELVHVARLTALGEMASALAHELNQPLSAAANYVKGSVRLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHEEEC
QEPLDKPKIKDALNLAGEQTLRAGQIIRRLRDFVAKGEAERSLESLPQLLEEAGALAMVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEC
AKERGVRLSFQISPEVGLVLADKVQIQQVVLNLMRNAIDAMEGSARRELVVSARPVEDEM
CCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCE
VEISVADTGPGVSPEVADHLFQPFITTKREGMGVGLSISRTIVEAHGGRIWVEPNPKGGA
EEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCE
IFRFTLRAVADEELTGIE
EEEEHHHHHCCCCCCCCC
>Mature Secondary Structure 
GPPDKLSPRAWWRGRVAGFGVAIGATAACLMLRLALEPVIHDRALFLLFVPALMAAAAV
CCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GGFAPALAATVVSLAIHAALMGDSLVSDTANLISAVVFALLGLLSGLAGSHLRKMVAETE
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HALEDLETREAHLRSILDTVPDAMIVIDEHGVMQSFSAAAERLFGWTAAESIGRNVSVLM
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
PNPYRDAHDGYLHRYLSTGERRIIGIGRVVVGERKDGSTFPMELSVGEMRVGERRFFTGF
CCCCCCCHHHHHHHHHHCCCCEEEEECEEEEECCCCCCCCCEEEECCCHHCCHHHHHHHH
VRDLTERQATERRLQDLQSELVHVARLTALGEMASALAHELNQPLSAAANYVKGSVRLLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHEEEC
QEPLDKPKIKDALNLAGEQTLRAGQIIRRLRDFVAKGEAERSLESLPQLLEEAGALAMVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEC
AKERGVRLSFQISPEVGLVLADKVQIQQVVLNLMRNAIDAMEGSARRELVVSARPVEDEM
CCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEECCCCCCCE
VEISVADTGPGVSPEVADHLFQPFITTKREGMGVGLSISRTIVEAHGGRIWVEPNPKGGA
EEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCE
IFRFTLRAVADEELTGIE
EEEEHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2046550; 1766374 [H]