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The map label for this gene is spoT [H]

Identifier: 197105343

GI number: 197105343

Start: 2106022

End: 2108190

Strand: Reverse

Name: spoT [H]

Synonym: PHZ_c1880

Alternate gene names: 197105343

Gene position: 2108190-2106022 (Counterclockwise)

Preceding gene: 197105344

Following gene: 197105340

Centisome position: 52.75

GC content: 70.08

Gene sequence:

>2169_bases
ATGCTGCGCCAGTTCGAGCTGATCGAGAAGGTCCGCTCCTACGACCCCACGGCCGACGAGGCGCTGCTGAACCGCGCCTA
CGTCTACGCCATGCGCCAGCACGGCTCGCAGAAGCGGGCCTCGGGCGACCCCTATTTCGCCCACCCGATCGAGGTGGCGG
GCATCCTCACCGACTACCGGCTGGACACCGAGACCATCGTCACGGCCCTGCTGCACGACGTCATCGAGGACACTGACGCC
ACCCGCGAGAAGGTGGAGGAGCTGTTCGGCGCCGAGATCGCCGAGCTGGTCGAGGGGGTCACCAAGCTCTCCAAGCTCGA
GCTCTCGTCCGAGCACCTGCGGCAGGCCGAGAACCTGCGCAAGTTCATCCTGGCGATCTCCAAGGACGTGCGCGTCCTGC
TCGTGAAGCTGGCCGACCGGCTTCACAACATGCGCACGCTGCACTTCATCAAGACGCCCGCCAAGCGCGAGCGGATCGCG
CGCGAGACGCTCGACATCTACGCCCCGCTGGCCCGCTCCATCGGCGTGCACCGGATCTGCGCCGAGCTGGAGGAGCTGGC
CTTCGCCCACCTCAATCCGGTGGCGCGCGATGCGATCATCCGCCGGCTCGAGGTGCTGCGCCAGCAGCAGGGCGGGGCGG
TCGCCCTGGTCAGCCAGGAGATCACCACCCGTCTCCAGGAGGCCGGCGTGCCCAGCCGGGTGTTCGGCCGCGAGAAGCAT
CCCTTCTCGATCTGGCGGAAGCTGCAGCGCAAGTCGATCGGCTTCTCCCAGCTCTCCGACATCTACGCCTTCCGGGTGAT
CGTGGACACCGAGGACGACTGCTACCGCGCGCTCGGCGTCATCCACCGCGCCTGGCCCAGCGTGCCCGAGCGCTTCAAGG
ACTTCATCTCCACGCCCAAGCGGAACAACTACCGCTCGCTGCACACCACCGTGGTCGGCCCCAAGGGGATGCGGATCGAG
ATGCAGATCCGCACCGAGTCCATGGACCAGGTCGCCGAGGAGGGCGTGGCCGCCCACTGGCGCTACAAGAACGAGAGCTA
CGGCTTCGACGCCGAGCACCAGGCCGCCGCCGGCGGCCGCGACCCGCTGGTCAACCTGCGCCACCTCGTCCAGGTGCTCG
AGCACGGCGGCGACGCCGAGGAGCTGGTCGAGCACGCCAAGCTCGAAATGTACCTCGACCAGGTGTTCGTCTTCACCCCC
AAGGGCCGGCTGGTCAGCCTGCCCCGGGGCGCGATGCCGCTGGACTTCGCCTACGCCGTCCACACCGACATCGGCGACAC
CACCATCGGCGTCAAAATCAACGGCGAACTCAAGCCCTTGCGCACGACTCTTCAGAACGGGGACGTGGTCGAGGTGATCC
GCGGCGGCAAGCCCGTGGTGCCGCCGGACTGGCGCTCGCTGACGGTCACCGGCCGGGCCCGCTCGGCCATCCGCCGCCAC
ATCCGCCAGACCGAGCGCGAGGAGTTCGTGCGCCTGGGCCGGGCCACGATCGACCAGGCCTTCGAGTCGGCCGGCAAGAG
CCGCAAGGACGTCTCGCTGCGTCCCGCCCTCGACCGCTTCGCCGTGGCCAGCGAGGAGGACCTGTTCGAGGCCATCGGCC
GCGGCCGCGCCCCGCCGGCCCAGGTGCTGGAGGCGGTCTTCCCGGGCCTGAAGGCGGCCGAGCGCGAGGCCGCCGCCGCC
CGGCGCCACATCCAGGACGGGGCCGGCGCGCGGCTCTACGTCCGCGGCGGGGGGCTCACCCCCGGCGTCTCGATCCATTT
CGGCGAGTGCTGCTCGCCCGTCCCCGGCGACCGGATCGTCGGCATCCTGGAGAGCGACGGCCGGGGCCTCACCGTCCACA
CCATTGACTGCGAGCGGCTGGCGGAGTTCGAGGAGCGCGAGGAGCTCTGGCGCGACCTGCAGTGGACGCCCGAGGCCGAG
CGCAACGCCGTGGCCCGGGCCCGCGTCACCGCCACCATCCGCAACGCCCCCGGGGTCCTGGGCCAGGTGTGCAGCATCAT
CGGCGAGGCCGGCGGCAACATCATGAACCTGCGCATGCACCACCGGCAGCAGGACTTCTTCGAGGTGGATATCGACGTGG
ACGTGCAGGACGCCAAGCACCTGACCCACATCGCCGCCGCCCTGCGCGCCAATCCCAGCGTCGAGACCGTCGAGCGGATG
AAGGGCTAG

Upstream 100 bases:

>100_bases
CGAGGCGGCGATCGAACCCGCCGCCCTCCCGGAAGCTCGCCCGGCCGAGCCCAAGGACTCTCCCGCCCAGCTGTCGGCCG
CCGTTGCCCGGCGGCCGAAG

Downstream 100 bases:

>100_bases
GGACCAGGGTCTCTAGGCTCCGCCGGCCTCCAACGCCCGCGCCACGCCGGCCAGCACCGGCCCCCAGTCGCCGGGCTGCG
TCTGCCGATGCAGCCTGGCG

Product: guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase [H]

Number of amino acids: Translated: 722; Mature: 722

Protein sequence:

>722_residues
MLRQFELIEKVRSYDPTADEALLNRAYVYAMRQHGSQKRASGDPYFAHPIEVAGILTDYRLDTETIVTALLHDVIEDTDA
TREKVEELFGAEIAELVEGVTKLSKLELSSEHLRQAENLRKFILAISKDVRVLLVKLADRLHNMRTLHFIKTPAKRERIA
RETLDIYAPLARSIGVHRICAELEELAFAHLNPVARDAIIRRLEVLRQQQGGAVALVSQEITTRLQEAGVPSRVFGREKH
PFSIWRKLQRKSIGFSQLSDIYAFRVIVDTEDDCYRALGVIHRAWPSVPERFKDFISTPKRNNYRSLHTTVVGPKGMRIE
MQIRTESMDQVAEEGVAAHWRYKNESYGFDAEHQAAAGGRDPLVNLRHLVQVLEHGGDAEELVEHAKLEMYLDQVFVFTP
KGRLVSLPRGAMPLDFAYAVHTDIGDTTIGVKINGELKPLRTTLQNGDVVEVIRGGKPVVPPDWRSLTVTGRARSAIRRH
IRQTEREEFVRLGRATIDQAFESAGKSRKDVSLRPALDRFAVASEEDLFEAIGRGRAPPAQVLEAVFPGLKAAEREAAAA
RRHIQDGAGARLYVRGGGLTPGVSIHFGECCSPVPGDRIVGILESDGRGLTVHTIDCERLAEFEEREELWRDLQWTPEAE
RNAVARARVTATIRNAPGVLGQVCSIIGEAGGNIMNLRMHHRQQDFFEVDIDVDVQDAKHLTHIAAALRANPSVETVERM
KG

Sequences:

>Translated_722_residues
MLRQFELIEKVRSYDPTADEALLNRAYVYAMRQHGSQKRASGDPYFAHPIEVAGILTDYRLDTETIVTALLHDVIEDTDA
TREKVEELFGAEIAELVEGVTKLSKLELSSEHLRQAENLRKFILAISKDVRVLLVKLADRLHNMRTLHFIKTPAKRERIA
RETLDIYAPLARSIGVHRICAELEELAFAHLNPVARDAIIRRLEVLRQQQGGAVALVSQEITTRLQEAGVPSRVFGREKH
PFSIWRKLQRKSIGFSQLSDIYAFRVIVDTEDDCYRALGVIHRAWPSVPERFKDFISTPKRNNYRSLHTTVVGPKGMRIE
MQIRTESMDQVAEEGVAAHWRYKNESYGFDAEHQAAAGGRDPLVNLRHLVQVLEHGGDAEELVEHAKLEMYLDQVFVFTP
KGRLVSLPRGAMPLDFAYAVHTDIGDTTIGVKINGELKPLRTTLQNGDVVEVIRGGKPVVPPDWRSLTVTGRARSAIRRH
IRQTEREEFVRLGRATIDQAFESAGKSRKDVSLRPALDRFAVASEEDLFEAIGRGRAPPAQVLEAVFPGLKAAEREAAAA
RRHIQDGAGARLYVRGGGLTPGVSIHFGECCSPVPGDRIVGILESDGRGLTVHTIDCERLAEFEEREELWRDLQWTPEAE
RNAVARARVTATIRNAPGVLGQVCSIIGEAGGNIMNLRMHHRQQDFFEVDIDVDVQDAKHLTHIAAALRANPSVETVERM
KG
>Mature_722_residues
MLRQFELIEKVRSYDPTADEALLNRAYVYAMRQHGSQKRASGDPYFAHPIEVAGILTDYRLDTETIVTALLHDVIEDTDA
TREKVEELFGAEIAELVEGVTKLSKLELSSEHLRQAENLRKFILAISKDVRVLLVKLADRLHNMRTLHFIKTPAKRERIA
RETLDIYAPLARSIGVHRICAELEELAFAHLNPVARDAIIRRLEVLRQQQGGAVALVSQEITTRLQEAGVPSRVFGREKH
PFSIWRKLQRKSIGFSQLSDIYAFRVIVDTEDDCYRALGVIHRAWPSVPERFKDFISTPKRNNYRSLHTTVVGPKGMRIE
MQIRTESMDQVAEEGVAAHWRYKNESYGFDAEHQAAAGGRDPLVNLRHLVQVLEHGGDAEELVEHAKLEMYLDQVFVFTP
KGRLVSLPRGAMPLDFAYAVHTDIGDTTIGVKINGELKPLRTTLQNGDVVEVIRGGKPVVPPDWRSLTVTGRARSAIRRH
IRQTEREEFVRLGRATIDQAFESAGKSRKDVSLRPALDRFAVASEEDLFEAIGRGRAPPAQVLEAVFPGLKAAEREAAAA
RRHIQDGAGARLYVRGGGLTPGVSIHFGECCSPVPGDRIVGILESDGRGLTVHTIDCERLAEFEEREELWRDLQWTPEAE
RNAVARARVTATIRNAPGVLGQVCSIIGEAGGNIMNLRMHHRQQDFFEVDIDVDVQDAKHLTHIAAALRANPSVETVERM
KG

Specific function: Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in ada

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=138, Percent_Identity=41.304347826087, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1790082, Length=723, Percent_Identity=39.9723374827109, Blast_Score=500, Evalue=1e-142,
Organism=Escherichia coli, GI1789147, Length=726, Percent_Identity=31.6804407713499, Blast_Score=365, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI25143118, Length=133, Percent_Identity=37.593984962406, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24650996, Length=130, Percent_Identity=40, Blast_Score=76, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 80940; Mature: 80940

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQFELIEKVRSYDPTADEALLNRAYVYAMRQHGSQKRASGDPYFAHPIEVAGILTDYR
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHHHCC
LDTETIVTALLHDVIEDTDATREKVEELFGAEIAELVEGVTKLSKLELSSEHLRQAENLR
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
KFILAISKDVRVLLVKLADRLHNMRTLHFIKTPAKRERIARETLDIYAPLARSIGVHRIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
AELEELAFAHLNPVARDAIIRRLEVLRQQQGGAVALVSQEITTRLQEAGVPSRVFGREKH
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCHHHHCCCCC
PFSIWRKLQRKSIGFSQLSDIYAFRVIVDTEDDCYRALGVIHRAWPSVPERFKDFISTPK
CHHHHHHHHHHCCCHHHHHHHEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
RNNYRSLHTTVVGPKGMRIEMQIRTESMDQVAEEGVAAHWRYKNESYGFDAEHQAAAGGR
CCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHCCCCC
DPLVNLRHLVQVLEHGGDAEELVEHAKLEMYLDQVFVFTPKGRLVSLPRGAMPLDFAYAV
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCCCCCEEE
HTDIGDTTIGVKINGELKPLRTTLQNGDVVEVIRGGKPVVPPDWRSLTVTGRARSAIRRH
ECCCCCEEEEEEECCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHH
IRQTEREEFVRLGRATIDQAFESAGKSRKDVSLRPALDRFAVASEEDLFEAIGRGRAPPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCHH
QVLEAVFPGLKAAEREAAAARRHIQDGAGARLYVRGGGLTPGVSIHFGECCSPVPGDRIV
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCEE
GILESDGRGLTVHTIDCERLAEFEEREELWRDLQWTPEAERNAVARARVTATIRNAPGVL
EEEECCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCCCHH
GQVCSIIGEAGGNIMNLRMHHRQQDFFEVDIDVDVQDAKHLTHIAAALRANPSVETVERM
HHHHHHHHHCCCCEEEEHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHH
KG
CC
>Mature Secondary Structure
MLRQFELIEKVRSYDPTADEALLNRAYVYAMRQHGSQKRASGDPYFAHPIEVAGILTDYR
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEECHHHHHHHHHHCC
LDTETIVTALLHDVIEDTDATREKVEELFGAEIAELVEGVTKLSKLELSSEHLRQAENLR
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
KFILAISKDVRVLLVKLADRLHNMRTLHFIKTPAKRERIARETLDIYAPLARSIGVHRIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHH
AELEELAFAHLNPVARDAIIRRLEVLRQQQGGAVALVSQEITTRLQEAGVPSRVFGREKH
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHCCCCHHHHCCCCC
PFSIWRKLQRKSIGFSQLSDIYAFRVIVDTEDDCYRALGVIHRAWPSVPERFKDFISTPK
CHHHHHHHHHHCCCHHHHHHHEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
RNNYRSLHTTVVGPKGMRIEMQIRTESMDQVAEEGVAAHWRYKNESYGFDAEHQAAAGGR
CCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHCCCCC
DPLVNLRHLVQVLEHGGDAEELVEHAKLEMYLDQVFVFTPKGRLVSLPRGAMPLDFAYAV
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCCCCCEEE
HTDIGDTTIGVKINGELKPLRTTLQNGDVVEVIRGGKPVVPPDWRSLTVTGRARSAIRRH
ECCCCCEEEEEEECCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHH
IRQTEREEFVRLGRATIDQAFESAGKSRKDVSLRPALDRFAVASEEDLFEAIGRGRAPPA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCHH
QVLEAVFPGLKAAEREAAAARRHIQDGAGARLYVRGGGLTPGVSIHFGECCSPVPGDRIV
HHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEECCCCCCCCCCCCEE
GILESDGRGLTVHTIDCERLAEFEEREELWRDLQWTPEAERNAVARARVTATIRNAPGVL
EEEECCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCCCHH
GQVCSIIGEAGGNIMNLRMHHRQQDFFEVDIDVDVQDAKHLTHIAAALRANPSVETVERM
HHHHHHHHHCCCCEEEEHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHH
KG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA