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The map label for this gene is lipA

Identifier: 197105200

GI number: 197105200

Start: 1946104

End: 1947063

Strand: Reverse

Name: lipA

Synonym: PHZ_c1737

Alternate gene names: 197105200

Gene position: 1947063-1946104 (Counterclockwise)

Preceding gene: 197105202

Following gene: 197105199

Centisome position: 48.72

GC content: 69.9

Gene sequence:

>960_bases
ATGGCCGTGGTCATCGACACCGTAGGCGCGCGTCCGCGCCACCCCGAGAAGCAGGCGAACCCCGACACGCCGGTCCTCCG
AAAGCCGGAATGGCTGCGGGTGAGGGCGCCGGGCTCGGCGAACTACATGGCCACGCGCGAGGTGGTGAAGTCCAACCGCC
TGGTCACGGTGTGCGAGGAGGCCGGCTGCCCCAACATCGGCGAGTGCTGGGACAAGAGCCACGCCACCTTCATGATCATG
GGCGAGGTCTGCACGCGGGCCTGCGCCTTCTGCAACGTGGCGACGGGCAAGCCTCTGGCGCTCGACCCGGACGAGCCGGC
GCGCGTGGGCGAGGCCACGGCCAAGATGGGGCTCAAGCACGTCGTGGTCACCTCGGTCGACCGCGACGACCTGGCCGACG
GGGGCGCCTGGCACTTCGTCGAGACGATCCGGGCGATCCGCGCCGCGTCGCCGGCGACCACCATCGAGATCCTGACGCCG
GACTTCGCCCGCAAGCCGGTCGCCGCCCTTGAGTCGGTGATCGACGCCCGGCCCGACGTCTTCAACCACAACCTCGAGAC
CGTGCCGCGGCTCTACCTGTCGATCCGCCCCGGCGCCCGCTACTACCACTCGCTGCGCCTGCTGGAGCGGGTGAAGGAGC
GCGACCCTACCCAGTTCACCAAGTCCGGCATCATGGTCGGCCTCGGCGAGAGCAAGGAGGAGGTCATGCAGGTGATGGAC
GACATGCGCTCGGCCGGCGTCGACTTCATCACCATCGGCCAGTACCTGCAGCCGACCCGCAAGCACGCCCCGATCGACCG
GTTCGTGCACCCGGACGAGTTCCGCGCGTTGGAGGAGATCGCCCGCGCGAAAGGCTTCCTGATGGTTTCGGCCAGCCCGC
TCACCCGCTCGTCGCATCACGCCGGCGAGGACTTCGCCCGCCTGCAGGCCGCGCGCCTCGCCAAGGAATCGGCGGCCTAG

Upstream 100 bases:

>100_bases
TCGGACTTTAAAATGAAAAGATCGGTTACAGAGCGTGAACGCCGCAGCCTGCGGCCATCCTCCGCTTGATCCCGGCGCTC
TGACGGACGATCTAGGGGCC

Downstream 100 bases:

>100_bases
GGCTTGCGGCACCACGTCTCCAAGGCGCTGCCCTATACGCCCGACCAGCTCTTCCAGCTGGTGGGGGACGTCGATCGCTA
CCCGGAGTTTGTACCCTGGG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MAVVIDTVGARPRHPEKQANPDTPVLRKPEWLRVRAPGSANYMATREVVKSNRLVTVCEEAGCPNIGECWDKSHATFMIM
GEVCTRACAFCNVATGKPLALDPDEPARVGEATAKMGLKHVVVTSVDRDDLADGGAWHFVETIRAIRAASPATTIEILTP
DFARKPVAALESVIDARPDVFNHNLETVPRLYLSIRPGARYYHSLRLLERVKERDPTQFTKSGIMVGLGESKEEVMQVMD
DMRSAGVDFITIGQYLQPTRKHAPIDRFVHPDEFRALEEIARAKGFLMVSASPLTRSSHHAGEDFARLQAARLAKESAA

Sequences:

>Translated_319_residues
MAVVIDTVGARPRHPEKQANPDTPVLRKPEWLRVRAPGSANYMATREVVKSNRLVTVCEEAGCPNIGECWDKSHATFMIM
GEVCTRACAFCNVATGKPLALDPDEPARVGEATAKMGLKHVVVTSVDRDDLADGGAWHFVETIRAIRAASPATTIEILTP
DFARKPVAALESVIDARPDVFNHNLETVPRLYLSIRPGARYYHSLRLLERVKERDPTQFTKSGIMVGLGESKEEVMQVMD
DMRSAGVDFITIGQYLQPTRKHAPIDRFVHPDEFRALEEIARAKGFLMVSASPLTRSSHHAGEDFARLQAARLAKESAA
>Mature_318_residues
AVVIDTVGARPRHPEKQANPDTPVLRKPEWLRVRAPGSANYMATREVVKSNRLVTVCEEAGCPNIGECWDKSHATFMIMG
EVCTRACAFCNVATGKPLALDPDEPARVGEATAKMGLKHVVVTSVDRDDLADGGAWHFVETIRAIRAASPATTIEILTPD
FARKPVAALESVIDARPDVFNHNLETVPRLYLSIRPGARYYHSLRLLERVKERDPTQFTKSGIMVGLGESKEEVMQVMDD
MRSAGVDFITIGQYLQPTRKHAPIDRFVHPDEFRALEEIARAKGFLMVSASPLTRSSHHAGEDFARLQAARLAKESAA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=299, Percent_Identity=43.4782608695652, Blast_Score=254, Evalue=8e-68,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=45.1612903225806, Blast_Score=220, Evalue=1e-57,
Organism=Escherichia coli, GI1786846, Length=278, Percent_Identity=51.7985611510791, Blast_Score=306, Evalue=9e-85,
Organism=Caenorhabditis elegans, GI32564533, Length=292, Percent_Identity=42.4657534246575, Blast_Score=236, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=299, Percent_Identity=44.4816053511706, Blast_Score=248, Evalue=6e-67,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=44.5993031358885, Blast_Score=254, Evalue=5e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_PHEZH (B4RBV1)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002130577.1
- GeneID:   6791250
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c1737
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35208; Mature: 35076

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVIDTVGARPRHPEKQANPDTPVLRKPEWLRVRAPGSANYMATREVVKSNRLVTVCEE
CEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECC
AGCPNIGECWDKSHATFMIMGEVCTRACAFCNVATGKPLALDPDEPARVGEATAKMGLKH
CCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCE
VVVTSVDRDDLADGGAWHFVETIRAIRAASPATTIEILTPDFARKPVAALESVIDARPDV
EEEEECCHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHCCHHHHHHHHHHCCCCH
FNHNLETVPRLYLSIRPGARYYHSLRLLERVKERDPTQFTKSGIMVGLGESKEEVMQVMD
HCCCHHHHHEEHEEECCCHHHHHHHHHHHHHHHCCCHHHHHCCEEEECCCCHHHHHHHHH
DMRSAGVDFITIGQYLQPTRKHAPIDRFVHPDEFRALEEIARAKGFLMVSASPLTRSSHH
HHHHCCCCEEECCHHHCCHHHCCCHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCC
AGEDFARLQAARLAKESAA
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVVIDTVGARPRHPEKQANPDTPVLRKPEWLRVRAPGSANYMATREVVKSNRLVTVCEE
EEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCEEEEECC
AGCPNIGECWDKSHATFMIMGEVCTRACAFCNVATGKPLALDPDEPARVGEATAKMGLKH
CCCCCHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHCCCE
VVVTSVDRDDLADGGAWHFVETIRAIRAASPATTIEILTPDFARKPVAALESVIDARPDV
EEEEECCHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHCCHHHHHHHHHHCCCCH
FNHNLETVPRLYLSIRPGARYYHSLRLLERVKERDPTQFTKSGIMVGLGESKEEVMQVMD
HCCCHHHHHEEHEEECCCHHHHHHHHHHHHHHHCCCHHHHHCCEEEECCCCHHHHHHHHH
DMRSAGVDFITIGQYLQPTRKHAPIDRFVHPDEFRALEEIARAKGFLMVSASPLTRSSHH
HHHHCCCCEEECCHHHCCHHHCCCHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCC
AGEDFARLQAARLAKESAA
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA