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The map label for this gene is lpd [H]

Identifier: 197105202

GI number: 197105202

Start: 1948797

End: 1950188

Strand: Reverse

Name: lpd [H]

Synonym: PHZ_c1739

Alternate gene names: 197105202

Gene position: 1950188-1948797 (Counterclockwise)

Preceding gene: 197105203

Following gene: 197105200

Centisome position: 48.8

GC content: 68.53

Gene sequence:

>1392_bases
ATGGCTGATGCGTTCGACGTCGTTGTGATCGGCTCTGGCCCCGGCGGCTACGTCACCGCCATCCGCGCCTCGCAGCTGGG
CTTCAAGACCGCCATCGTGGAGCGGGCCGAGCTGGGCGGCATCTGCCTGAACTGGGGCTGCATCCCCACCAAGGCGCTGC
TGAAGTCCGGCGAGGTCTACGAACAGCTCGACCACCTGAAGGACTACGGCCTGTCGGTGGAGAAGCGCGGCTTCGATTTC
GAGGCCGTGGTCCAGCGGTCCCGCAAGGTCGCCGCCCAGATGAATTCGGGCGTCACCTTCCTGATGAAGAAGCACAAGAT
CGAGGTGGTGCAGGGGACGGCGCGGCTGGAGAAGGGCTCGCCTGCGCCGACGGTCGTGGTCGGCCAGCGCAAGCTGACCG
CCAAGCATGTGATCCTGGCCACCGGCGCGCGGGCGCGCACCGTCCCCGCCGTCGGCCTGGAGCCGGACGGCGAGCGGGTC
TGGACCTACCGCGAGGCCCTGGTCCCCAAGGCTGCCCCCAAGTCGCTGGTGGTCGTCGGCTCCGGCGCCATCGGCATGGA
GTTCGCCAGCTTCTACAGGGCCTTGGGCTCGGAAGTTACGGTGATCGAGATGATGCCGCGCATCCTCCCGGTGGAGGACG
AGGAGATCTCCAAGACCGCCCGCAAGGCCTTCGAGAAGCGGGGCATCAGGTTCCGCGTGCCGGCCAACGTGAAGAAGCTG
ACCAAGGGCAAGGGCGGCGTCAGCCTGGAGATCGAGGCCGACGGCAAGGCCGAGACCCTGCAGGCCGACGTCTGCATCGT
GGCCATCGGGATCGTCGGCAACGTCGAGGACCTCGGCCTTGAGGCCCTCGGTGTGAAGATCGAGAAGACCCACGTGGTCA
CCGACAAGCACGGGGCCACCGGCGTGCCCGGCCTCTACGCCATCGGCGACGTGGCCGGCCCGCCTTGGCTGGCCCACAAG
GCGAGCCACGAGGGCGTCCACTGCATCGAGCACATCGCCGGCCTGAAGCCCACGAACCTGACCGCGCCGATTCCGGGCTG
CACCTACACCACGCCGCAGATCGCCTCGGTGGGCCTGACCGAGGCCCAGGCGAAGGAACAGGGGCTGGAGCCCAAGGTCG
GCCGCTTCCCGTTCCGGGTGAACGGCAAGGCGGTGGCGGCCGGCGAGCCTGACGGCCTGGTGAAGACCATCTTCGACGGC
AAGACCGGCGCGCTGATCGGCGCCCACATGGTCGGCCATGAGGTCACCGAGATGATCCAGGGCTTCGCCATGGCGATGAC
GCTGGAGGCCACCGAGGCCGAGATCCAGGCGACGGTCTTCCCGCACCCGACCATGAGCGAGGCCATGCACGAGGCCGCGC
TCGACGCCTACGGGCGGGTTCTGCACATCTGA

Upstream 100 bases:

>100_bases
TCACCAAGTTCCTCGTGGACCGGTCGGGGAAGGTCGTGGACCGCTTCGCCCCCACCGTTGAACCCAAGGCGCTGGAAGGC
GCGATCGAGGCTTTGCTCTG

Downstream 100 bases:

>100_bases
CGCTGCTTCCCTCCCTTGAACGGGAGGGAGGACTTACGCCGCCCGCCGCAGCTCGGCTTCCACGATCCTCTGGAGCGCCA
CGTAGTCGGTCTTGCCGGTC

Product: pyruvate dehydrogenase complex, E3 component, lipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MADAFDVVVIGSGPGGYVTAIRASQLGFKTAIVERAELGGICLNWGCIPTKALLKSGEVYEQLDHLKDYGLSVEKRGFDF
EAVVQRSRKVAAQMNSGVTFLMKKHKIEVVQGTARLEKGSPAPTVVVGQRKLTAKHVILATGARARTVPAVGLEPDGERV
WTYREALVPKAAPKSLVVVGSGAIGMEFASFYRALGSEVTVIEMMPRILPVEDEEISKTARKAFEKRGIRFRVPANVKKL
TKGKGGVSLEIEADGKAETLQADVCIVAIGIVGNVEDLGLEALGVKIEKTHVVTDKHGATGVPGLYAIGDVAGPPWLAHK
ASHEGVHCIEHIAGLKPTNLTAPIPGCTYTTPQIASVGLTEAQAKEQGLEPKVGRFPFRVNGKAVAAGEPDGLVKTIFDG
KTGALIGAHMVGHEVTEMIQGFAMAMTLEATEAEIQATVFPHPTMSEAMHEAALDAYGRVLHI

Sequences:

>Translated_463_residues
MADAFDVVVIGSGPGGYVTAIRASQLGFKTAIVERAELGGICLNWGCIPTKALLKSGEVYEQLDHLKDYGLSVEKRGFDF
EAVVQRSRKVAAQMNSGVTFLMKKHKIEVVQGTARLEKGSPAPTVVVGQRKLTAKHVILATGARARTVPAVGLEPDGERV
WTYREALVPKAAPKSLVVVGSGAIGMEFASFYRALGSEVTVIEMMPRILPVEDEEISKTARKAFEKRGIRFRVPANVKKL
TKGKGGVSLEIEADGKAETLQADVCIVAIGIVGNVEDLGLEALGVKIEKTHVVTDKHGATGVPGLYAIGDVAGPPWLAHK
ASHEGVHCIEHIAGLKPTNLTAPIPGCTYTTPQIASVGLTEAQAKEQGLEPKVGRFPFRVNGKAVAAGEPDGLVKTIFDG
KTGALIGAHMVGHEVTEMIQGFAMAMTLEATEAEIQATVFPHPTMSEAMHEAALDAYGRVLHI
>Mature_462_residues
ADAFDVVVIGSGPGGYVTAIRASQLGFKTAIVERAELGGICLNWGCIPTKALLKSGEVYEQLDHLKDYGLSVEKRGFDFE
AVVQRSRKVAAQMNSGVTFLMKKHKIEVVQGTARLEKGSPAPTVVVGQRKLTAKHVILATGARARTVPAVGLEPDGERVW
TYREALVPKAAPKSLVVVGSGAIGMEFASFYRALGSEVTVIEMMPRILPVEDEEISKTARKAFEKRGIRFRVPANVKKLT
KGKGGVSLEIEADGKAETLQADVCIVAIGIVGNVEDLGLEALGVKIEKTHVVTDKHGATGVPGLYAIGDVAGPPWLAHKA
SHEGVHCIEHIAGLKPTNLTAPIPGCTYTTPQIASVGLTEAQAKEQGLEPKVGRFPFRVNGKAVAAGEPDGLVKTIFDGK
TGALIGAHMVGHEVTEMIQGFAMAMTLEATEAEIQATVFPHPTMSEAMHEAALDAYGRVLHI

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=36.5957446808511, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI50301238, Length=464, Percent_Identity=31.0344827586207, Blast_Score=182, Evalue=8e-46,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=27.5862068965517, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI33519430, Length=464, Percent_Identity=26.2931034482759, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI33519428, Length=464, Percent_Identity=26.2931034482759, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI33519426, Length=464, Percent_Identity=26.2931034482759, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI148277065, Length=464, Percent_Identity=26.2931034482759, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI148277071, Length=464, Percent_Identity=26.2931034482759, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=26.1574074074074, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI291045268, Length=424, Percent_Identity=24.2924528301887, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1786307, Length=454, Percent_Identity=35.6828193832599, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1789915, Length=438, Percent_Identity=31.5068493150685, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=28.3582089552239, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI87081717, Length=458, Percent_Identity=25.3275109170306, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI1788892, Length=191, Percent_Identity=32.4607329842932, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=470, Percent_Identity=35.531914893617, Blast_Score=270, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI71983419, Length=458, Percent_Identity=30.1310043668122, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=458, Percent_Identity=30.1310043668122, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17557007, Length=480, Percent_Identity=24.7916666666667, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=501, Percent_Identity=24.9500998003992, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17559934, Length=226, Percent_Identity=31.4159292035398, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=36.0995850622407, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6325240, Length=480, Percent_Identity=27.7083333333333, Blast_Score=170, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6325166, Length=466, Percent_Identity=28.9699570815451, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21358499, Length=474, Percent_Identity=36.9198312236287, Blast_Score=268, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24640553, Length=475, Percent_Identity=28.2105263157895, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24640549, Length=475, Percent_Identity=28.2105263157895, Blast_Score=128, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24640551, Length=475, Percent_Identity=28.2105263157895, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17737741, Length=472, Percent_Identity=26.9067796610169, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49144; Mature: 49012

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADAFDVVVIGSGPGGYVTAIRASQLGFKTAIVERAELGGICLNWGCIPTKALLKSGEVY
CCCCEEEEEEECCCCCEEEEEEHHHCCHHHHHHHHHHCCCEEEECCCCCHHHHHCCCHHH
EQLDHLKDYGLSVEKRGFDFEAVVQRSRKVAAQMNSGVTFLMKKHKIEVVQGTARLEKGS
HHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCHHHCCCC
PAPTVVVGQRKLTAKHVILATGARARTVPAVGLEPDGERVWTYREALVPKAAPKSLVVVG
CCCEEEECCCCCCEEEEEEECCCCCCCCCEECCCCCCCEEEEHHHHHCCCCCCCCEEEEE
SGAIGMEFASFYRALGSEVTVIEMMPRILPVEDEEISKTARKAFEKRGIRFRVPANVKKL
CCCHHHHHHHHHHHHCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHH
TKGKGGVSLEIEADGKAETLQADVCIVAIGIVGNVEDLGLEALGVKIEKTHVVTDKHGAT
HCCCCCEEEEEECCCCCHHEECCEEEEEEEEECCHHHCCHHHHCEEEEEEEEEECCCCCC
GVPGLYAIGDVAGPPWLAHKASHEGVHCIEHIAGLKPTNLTAPIPGCTYTTPQIASVGLT
CCCCCEEECCCCCCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCC
EAQAKEQGLEPKVGRFPFRVNGKAVAAGEPDGLVKTIFDGKTGALIGAHMVGHEVTEMIQ
HHHHHHCCCCCCCCCCCEEECCEEEECCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHH
GFAMAMTLEATEAEIQATVFPHPTMSEAMHEAALDAYGRVLHI
HHHEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCEECC
>Mature Secondary Structure 
ADAFDVVVIGSGPGGYVTAIRASQLGFKTAIVERAELGGICLNWGCIPTKALLKSGEVY
CCCEEEEEEECCCCCEEEEEEHHHCCHHHHHHHHHHCCCEEEECCCCCHHHHHCCCHHH
EQLDHLKDYGLSVEKRGFDFEAVVQRSRKVAAQMNSGVTFLMKKHKIEVVQGTARLEKGS
HHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCHHHCCCC
PAPTVVVGQRKLTAKHVILATGARARTVPAVGLEPDGERVWTYREALVPKAAPKSLVVVG
CCCEEEECCCCCCEEEEEEECCCCCCCCCEECCCCCCCEEEEHHHHHCCCCCCCCEEEEE
SGAIGMEFASFYRALGSEVTVIEMMPRILPVEDEEISKTARKAFEKRGIRFRVPANVKKL
CCCHHHHHHHHHHHHCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEECCCCHHHH
TKGKGGVSLEIEADGKAETLQADVCIVAIGIVGNVEDLGLEALGVKIEKTHVVTDKHGAT
HCCCCCEEEEEECCCCCHHEECCEEEEEEEEECCHHHCCHHHHCEEEEEEEEEECCCCCC
GVPGLYAIGDVAGPPWLAHKASHEGVHCIEHIAGLKPTNLTAPIPGCTYTTPQIASVGLT
CCCCCEEECCCCCCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCC
EAQAKEQGLEPKVGRFPFRVNGKAVAAGEPDGLVKTIFDGKTGALIGAHMVGHEVTEMIQ
HHHHHHCCCCCCCCCCCEEECCEEEECCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHH
GFAMAMTLEATEAEIQATVFPHPTMSEAMHEAALDAYGRVLHI
HHHEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]