Click here to switch to the map view.

The map label for this gene is cnrH [H]

Identifier: 197104940

GI number: 197104940

Start: 1676920

End: 1677495

Strand: Direct

Name: cnrH [H]

Synonym: PHZ_c1475

Alternate gene names: 197104940

Gene position: 1676920-1677495 (Clockwise)

Preceding gene: 197104939

Following gene: 197104941

Centisome position: 41.96

GC content: 71.88

Gene sequence:

>576_bases
GTGAGCGCCGCCGCAGACGAGAGCGACGCGGCGCTCGCGACCCGTGCGGCCGGTGGCGACGACCAGGCCTATGCCGCCCT
GATGCGGCGCCACAAGGCCAGCCTCTACAGCTTCGTCCGTCGCTACATCGGCGATCCGGAGGCGGCCCTCGACGTCCTCC
AGGAGACCTTCGTGGCGGCGTGGCAGGCGATCCGCCGCTACGACGGCGGGCGGCCGTTCGCGACCTGGCTGCGCGCCATC
GCCCTCAACAAGTGCCGCGATCGCGGCCGGCGCCTGGCGGTCCGTCGGCTGGTGTTCGGAGCGACGGACCTGGACTCCGC
GGAGGCGCGGCGCCAGGCCGATCCGCAGGGCGACGCCGAGGGCGAACTCCAGTCCGCCCAACGCTTCGCCGCCCTGGACC
GCGCCATCGCCCGGCTTCCCGACAAGCTCAAGGTTCCGCTCATCCTGACCCAGCTGGAGGGGTTCAGCCAGCAGGCCGCG
GCCGAGCAGCTCGGGGTCAGCGTCAAGACCATCGAGACCAGGATCTATCGCGCCCGGCGCCGACTTGCCGGCGAACTCGG
CGATCTGGAAATTTGA

Upstream 100 bases:

>100_bases
AGACCATCCTTCATGTCCTCGCCATGCGGACCGTGCTGACGCCCGAGCAGGCCGTGAAGTTCGATCGCCGCATCAGCGAG
GCGCTGACCGACCAGGCCCT

Downstream 100 bases:

>100_bases
GCGATCGCGAGGGATAGACGCCTGCGCTGCGTATACGTCATCGCAGGCCCTCCCTTGACCCTCCCTACGTGGGAAGCGCT
ATCCCTCCGCCAATGAGGAC

Product: RNA polymerase sigma-70 factor,ECF subfamily

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MSAAADESDAALATRAAGGDDQAYAALMRRHKASLYSFVRRYIGDPEAALDVLQETFVAAWQAIRRYDGGRPFATWLRAI
ALNKCRDRGRRLAVRRLVFGATDLDSAEARRQADPQGDAEGELQSAQRFAALDRAIARLPDKLKVPLILTQLEGFSQQAA
AEQLGVSVKTIETRIYRARRRLAGELGDLEI

Sequences:

>Translated_191_residues
MSAAADESDAALATRAAGGDDQAYAALMRRHKASLYSFVRRYIGDPEAALDVLQETFVAAWQAIRRYDGGRPFATWLRAI
ALNKCRDRGRRLAVRRLVFGATDLDSAEARRQADPQGDAEGELQSAQRFAALDRAIARLPDKLKVPLILTQLEGFSQQAA
AEQLGVSVKTIETRIYRARRRLAGELGDLEI
>Mature_190_residues
SAAADESDAALATRAAGGDDQAYAALMRRHKASLYSFVRRYIGDPEAALDVLQETFVAAWQAIRRYDGGRPFATWLRAIA
LNKCRDRGRRLAVRRLVFGATDLDSAEARRQADPQGDAEGELQSAQRFAALDRAIARLPDKLKVPLILTQLEGFSQQAAA
EQLGVSVKTIETRIYRARRRLAGELGDLEI

Specific function: CnrH alone is able to activate CNR expression, while both CnrY and CrnX are needed for nickel induction of cnrH (PubMed:10671463). Binds DNA in a RNA polymerase-dependent fashion (PubMed:10671464). CnrH may be controlled by a CnrYX transmembrane anti-sigm

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=193, Percent_Identity=28.4974093264249, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21073; Mature: 20942

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAADESDAALATRAAGGDDQAYAALMRRHKASLYSFVRRYIGDPEAALDVLQETFVAA
CCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
WQAIRRYDGGRPFATWLRAIALNKCRDRGRRLAVRRLVFGATDLDSAEARRQADPQGDAE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
GELQSAQRFAALDRAIARLPDKLKVPLILTQLEGFSQQAAAEQLGVSVKTIETRIYRARR
HHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHH
RLAGELGDLEI
HHHHHCCCCCC
>Mature Secondary Structure 
SAAADESDAALATRAAGGDDQAYAALMRRHKASLYSFVRRYIGDPEAALDVLQETFVAA
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
WQAIRRYDGGRPFATWLRAIALNKCRDRGRRLAVRRLVFGATDLDSAEARRQADPQGDAE
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCC
GELQSAQRFAALDRAIARLPDKLKVPLILTQLEGFSQQAAAEQLGVSVKTIETRIYRARR
HHHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHHH
RLAGELGDLEI
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8380802; 8052622; 10671463; 10671464 [H]