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The map label for this gene is gatA [H]

Identifier: 197104619

GI number: 197104619

Start: 1322442

End: 1323719

Strand: Reverse

Name: gatA [H]

Synonym: PHZ_c1153

Alternate gene names: 197104619

Gene position: 1323719-1322442 (Counterclockwise)

Preceding gene: 197104622

Following gene: 197104616

Centisome position: 33.12

GC content: 75.74

Gene sequence:

>1278_bases
ATGACCCCCGCCGAGGCCCTCGCCCGCATCCGCACCTACGATCCCCGGCTGAAGGCCTTCGTGCGGGTCTTCGATCCGCC
GCTCCTGGGCGAGGACCTGGACGGGCCCACCTTCGCCATCAAGGACCTGTTCGACGTCGCGGGCGTGCCCACCGGCGGCG
GCGCCCAGGTTCCGCTCGACCCCAGCCCGACCCGCCACTCGCGCGCCGTCGAGCGCCTGCTGCAGGCCGGCTGGAGCGCG
GTCGGCAAGACCCACACGGTGGAGCTGGCCTATGGCGGCTGGGGCACCAACCGGGCCGTGGGCGCGCCCTGGAATCCCTG
GGATCCCAAGGTCCACCGCGCGCCCGGCGGATCCTCCTCGGGCTCGGCCGTGGCGGTCGCGGCGGGGCTCTGCGACGCCG
CCCTCGGCTCGGACACCGGCGGGTCGGTGCGCATCCCGGCGGCGGTCTGCGGCGTGGTGGGGCTGAAGCCCGGCCGCGGC
CTCGTCAGCCTGCGCGGCGTCCATCCGCTCAGCCCCGCGCTGGACACCGTGGGAACGCTCGCGCGGTCTGTCGAGATGGC
CGCCCGCGCCCTGGCCGCCATCTCGGGCCCCGACGGCGCGGCCGCCGTCCGCGAGCCGTTCGACTCCGAAGCCGCCCTGT
CGATGGATGTGCGCGGACGCCGCGTCGCGGCCATTCCCCTCGACGACCTCGGCGAGGTCCACCCCGACGTGGGCCGCCTC
TACCTCGAGGCCATCGAGCGGTTGCGCTCGGCCGGCGTCGCCGTCGAGATGGTGCGCCCGCCCTCGGCCCTGGAGCAGTC
CTTCGCGCCCAATGGCCTGCTGATGGCCGGGGAAGGCTGGCGCATCTGGGGCGAGCGCATCCAGCGCCATGCCGAGGTGA
TGGACCCTTGGATCGTCCGCCGCTTCGAGGCCGGCCGCGACGTCACCGACCAGCGTCTCGAGGACGTCCACCGCCGCCGG
GCCGAGGACCAGGCCGCCTTCCACGCCTGGATCGCCGGCTGGGACGGCCTGCTCTCGCCCACCTGCCCCATCCCCGCGCC
CCCGATCGACGAGGTGGACGAGACCGTCTCGCCCCTCAGCCGCCTGACGCGGGCGGCCAACTACCTCGACCTGCCGGGGA
TCAGCGTGCCCTGCGGCCTCACCGGCGAGGGCCTGCCGGCGGGGCTGCAGATCCTGGGTCGGCCGCGGGACGAGGCCTCG
GTCGTGGCCCTGGGCGCGGCCTTCGAGCGCGTGTCGGGCTGGGATGGCCGCGCGCCGGACCTCGCCGGCTTCGCCTAG

Upstream 100 bases:

>100_bases
CGCAGCTGGTCCGGAACCGACTGGGTCATGGTTCGCCTTTAAGCCTCCCGCCGCGCAGGCGTCATCTGGTTTCGCGACCG
CCGGTCGGCGATAAGGGGGC

Downstream 100 bases:

>100_bases
CCGCGGACGCGCCCGCCGATCCACCGCAGGACGATCCAGGCGCCCAGCGCCCAGACCGCGCCGAGGCACCAGCCGGCCAG
GACGTCGCTGGGCCAGTGGA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTPAEALARIRTYDPRLKAFVRVFDPPLLGEDLDGPTFAIKDLFDVAGVPTGGGAQVPLDPSPTRHSRAVERLLQAGWSA
VGKTHTVELAYGGWGTNRAVGAPWNPWDPKVHRAPGGSSSGSAVAVAAGLCDAALGSDTGGSVRIPAAVCGVVGLKPGRG
LVSLRGVHPLSPALDTVGTLARSVEMAARALAAISGPDGAAAVREPFDSEAALSMDVRGRRVAAIPLDDLGEVHPDVGRL
YLEAIERLRSAGVAVEMVRPPSALEQSFAPNGLLMAGEGWRIWGERIQRHAEVMDPWIVRRFEAGRDVTDQRLEDVHRRR
AEDQAAFHAWIAGWDGLLSPTCPIPAPPIDEVDETVSPLSRLTRAANYLDLPGISVPCGLTGEGLPAGLQILGRPRDEAS
VVALGAAFERVSGWDGRAPDLAGFA

Sequences:

>Translated_425_residues
MTPAEALARIRTYDPRLKAFVRVFDPPLLGEDLDGPTFAIKDLFDVAGVPTGGGAQVPLDPSPTRHSRAVERLLQAGWSA
VGKTHTVELAYGGWGTNRAVGAPWNPWDPKVHRAPGGSSSGSAVAVAAGLCDAALGSDTGGSVRIPAAVCGVVGLKPGRG
LVSLRGVHPLSPALDTVGTLARSVEMAARALAAISGPDGAAAVREPFDSEAALSMDVRGRRVAAIPLDDLGEVHPDVGRL
YLEAIERLRSAGVAVEMVRPPSALEQSFAPNGLLMAGEGWRIWGERIQRHAEVMDPWIVRRFEAGRDVTDQRLEDVHRRR
AEDQAAFHAWIAGWDGLLSPTCPIPAPPIDEVDETVSPLSRLTRAANYLDLPGISVPCGLTGEGLPAGLQILGRPRDEAS
VVALGAAFERVSGWDGRAPDLAGFA
>Mature_424_residues
TPAEALARIRTYDPRLKAFVRVFDPPLLGEDLDGPTFAIKDLFDVAGVPTGGGAQVPLDPSPTRHSRAVERLLQAGWSAV
GKTHTVELAYGGWGTNRAVGAPWNPWDPKVHRAPGGSSSGSAVAVAAGLCDAALGSDTGGSVRIPAAVCGVVGLKPGRGL
VSLRGVHPLSPALDTVGTLARSVEMAARALAAISGPDGAAAVREPFDSEAALSMDVRGRRVAAIPLDDLGEVHPDVGRLY
LEAIERLRSAGVAVEMVRPPSALEQSFAPNGLLMAGEGWRIWGERIQRHAEVMDPWIVRRFEAGRDVTDQRLEDVHRRRA
EDQAAFHAWIAGWDGLLSPTCPIPAPPIDEVDETVSPLSRLTRAANYLDLPGISVPCGLTGEGLPAGLQILGRPRDEASV
VALGAAFERVSGWDGRAPDLAGFA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=434, Percent_Identity=31.3364055299539, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI195972892, Length=418, Percent_Identity=26.0765550239234, Blast_Score=85, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=396, Percent_Identity=28.5353535353535, Blast_Score=111, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=242, Percent_Identity=30.1652892561983, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=429, Percent_Identity=25.4079254079254, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=390, Percent_Identity=30.7692307692308, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323950, Length=456, Percent_Identity=25.4385964912281, Blast_Score=93, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648113, Length=429, Percent_Identity=30.5361305361305, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI21356731, Length=409, Percent_Identity=23.4718826405868, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI161078093, Length=388, Percent_Identity=26.8041237113402, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648435, Length=136, Percent_Identity=34.5588235294118, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24648437, Length=136, Percent_Identity=34.5588235294118, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI45550774, Length=136, Percent_Identity=34.5588235294118, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 44736; Mature: 44605

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAEALARIRTYDPRLKAFVRVFDPPLLGEDLDGPTFAIKDLFDVAGVPTGGGAQVPLD
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
PSPTRHSRAVERLLQAGWSAVGKTHTVELAYGGWGTNRAVGAPWNPWDPKVHRAPGGSSS
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCC
GSAVAVAAGLCDAALGSDTGGSVRIPAAVCGVVGLKPGRGLVSLRGVHPLSPALDTVGTL
CCEEEHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHH
ARSVEMAARALAAISGPDGAAAVREPFDSEAALSMDVRGRRVAAIPLDDLGEVHPDVGRL
HHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECCCCCEEEECCCHHHHHCCHHHHHH
YLEAIERLRSAGVAVEMVRPPSALEQSFAPNGLLMAGEGWRIWGERIQRHAEVMDPWIVR
HHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCHHHHH
RFEAGRDVTDQRLEDVHRRRAEDQAAFHAWIAGWDGLLSPTCPIPAPPIDEVDETVSPLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RLTRAANYLDLPGISVPCGLTGEGLPAGLQILGRPRDEASVVALGAAFERVSGWDGRAPD
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHEEEHHHHHHHHCCCCCCCCC
LAGFA
CCCCC
>Mature Secondary Structure 
TPAEALARIRTYDPRLKAFVRVFDPPLLGEDLDGPTFAIKDLFDVAGVPTGGGAQVPLD
CHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCC
PSPTRHSRAVERLLQAGWSAVGKTHTVELAYGGWGTNRAVGAPWNPWDPKVHRAPGGSSS
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCC
GSAVAVAAGLCDAALGSDTGGSVRIPAAVCGVVGLKPGRGLVSLRGVHPLSPALDTVGTL
CCEEEHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHH
ARSVEMAARALAAISGPDGAAAVREPFDSEAALSMDVRGRRVAAIPLDDLGEVHPDVGRL
HHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEECCCCCEEEECCCHHHHHCCHHHHHH
YLEAIERLRSAGVAVEMVRPPSALEQSFAPNGLLMAGEGWRIWGERIQRHAEVMDPWIVR
HHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHCHHHHH
RFEAGRDVTDQRLEDVHRRRAEDQAAFHAWIAGWDGLLSPTCPIPAPPIDEVDETVSPLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RLTRAANYLDLPGISVPCGLTGEGLPAGLQILGRPRDEASVVALGAAFERVSGWDGRAPD
HHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHEEEHHHHHHHHCCCCCCCCC
LAGFA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]