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The map label for this gene is fixK [H]

Identifier: 197104622

GI number: 197104622

Start: 1327869

End: 1328651

Strand: Reverse

Name: fixK [H]

Synonym: PHZ_c1156

Alternate gene names: 197104622

Gene position: 1328651-1327869 (Counterclockwise)

Preceding gene: 197104624

Following gene: 197104619

Centisome position: 33.25

GC content: 73.05

Gene sequence:

>783_bases
ATGACGGGCCGGTTCAGACCTCGCCTCGCCGCATGGCCCATAGATCCGGTCGTGCGGAAGCTGCGGTCGCTTACACCCCT
GCCGGAGGCCGAAGAGGCGTTCGTCCGAAGCCTGAGCGAACGCCGGGAGCGGTTCGCCCCCGGCGAGGAGCTGATCCCCG
AGGGTCAGCCCGCGCGCCGGCCCAGGTTCGTTGTGGCCGGCTGGGCCGCCTGCCAGCGCGTGCTGCCGGACGGCCGGCGC
CAGATCTTCCGGCTGATCCTGCCCGGCGACCACTTCGGGTTCTGCGACAGGGCCGCGCCGCCCTCGCCCTGGAGCGTGGT
CGCGCTCACCGCCCTCGAGACGGTCGACGCCGAGGCCGCCCTGGACGCCGTCCTCGACGAGCGCACGCCCGCACTGGGCC
AGGCGATGGCCGCCGCCGCGGCGCAGGAACAGCAGCTCCTGCTGGACCACATGGTGCGGCTCGGCCAGCAAACCGCCTAC
GAACGGGTCGCCCACTTCCTGCTCGAGCTTCAAAGGCGGCTGCACGTCGCCGGGCTCGGCGACAACCAGCGCTTCCCGCT
GCCGCTCACCCAGGAAATCCTGGCCGACGCCCTCGGGCTGTCGATCGTCCACGTGAACCGCACGCTGCAGCAGCTGCGCA
AGGAGCGGCTGATCGAGCTGCGGTCGGGCGTGGCCATCCTGCTGCAGCCGGACGTCCTGGCCGAGGTCGCCCACTACGAG
CCGGGGCCCGCGGTGCGGGCCCCCTCGCCGGCCCGCCGTCAGCTGGAGAAGACGTCGGCGTAG

Upstream 100 bases:

>100_bases
CCTTCCTGGCGCGTATCGACTTTGTCCGGGGGTCGTGTCTGCGCTCAGGCGCAATCGGTTTCCGGACCTCGCGTAGCCTT
TAGCCCATGAGGAGCACCGG

Downstream 100 bases:

>100_bases
CGCTCGCGCAGCAGGTTCTTCTGGACCTTGCCCATGGCGTTGCGGGGCAGTTCATCGACGAAAAGGACGCGCTTGGGCGC
CTTGTAGGCGGCGAGCTGCC

Product: transcriptional regulator, Crp/Fnr family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTGRFRPRLAAWPIDPVVRKLRSLTPLPEAEEAFVRSLSERRERFAPGEELIPEGQPARRPRFVVAGWAACQRVLPDGRR
QIFRLILPGDHFGFCDRAAPPSPWSVVALTALETVDAEAALDAVLDERTPALGQAMAAAAAQEQQLLLDHMVRLGQQTAY
ERVAHFLLELQRRLHVAGLGDNQRFPLPLTQEILADALGLSIVHVNRTLQQLRKERLIELRSGVAILLQPDVLAEVAHYE
PGPAVRAPSPARRQLEKTSA

Sequences:

>Translated_260_residues
MTGRFRPRLAAWPIDPVVRKLRSLTPLPEAEEAFVRSLSERRERFAPGEELIPEGQPARRPRFVVAGWAACQRVLPDGRR
QIFRLILPGDHFGFCDRAAPPSPWSVVALTALETVDAEAALDAVLDERTPALGQAMAAAAAQEQQLLLDHMVRLGQQTAY
ERVAHFLLELQRRLHVAGLGDNQRFPLPLTQEILADALGLSIVHVNRTLQQLRKERLIELRSGVAILLQPDVLAEVAHYE
PGPAVRAPSPARRQLEKTSA
>Mature_259_residues
TGRFRPRLAAWPIDPVVRKLRSLTPLPEAEEAFVRSLSERRERFAPGEELIPEGQPARRPRFVVAGWAACQRVLPDGRRQ
IFRLILPGDHFGFCDRAAPPSPWSVVALTALETVDAEAALDAVLDERTPALGQAMAAAAAQEQQLLLDHMVRLGQQTAYE
RVAHFLLELQRRLHVAGLGDNQRFPLPLTQEILADALGLSIVHVNRTLQQLRKERLIELRSGVAILLQPDVLAEVAHYEP
GPAVRAPSPARRQLEKTSA

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28898; Mature: 28767

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRFRPRLAAWPIDPVVRKLRSLTPLPEAEEAFVRSLSERRERFAPGEELIPEGQPARR
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCC
PRFVVAGWAACQRVLPDGRRQIFRLILPGDHFGFCDRAAPPSPWSVVALTALETVDAEAA
CCEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LDAVLDERTPALGQAMAAAAAQEQQLLLDHMVRLGQQTAYERVAHFLLELQRRLHVAGLG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DNQRFPLPLTQEILADALGLSIVHVNRTLQQLRKERLIELRSGVAILLQPDVLAEVAHYE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCC
PGPAVRAPSPARRQLEKTSA
CCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TGRFRPRLAAWPIDPVVRKLRSLTPLPEAEEAFVRSLSERRERFAPGEELIPEGQPARR
CCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCC
PRFVVAGWAACQRVLPDGRRQIFRLILPGDHFGFCDRAAPPSPWSVVALTALETVDAEAA
CCEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LDAVLDERTPALGQAMAAAAAQEQQLLLDHMVRLGQQTAYERVAHFLLELQRRLHVAGLG
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DNQRFPLPLTQEILADALGLSIVHVNRTLQQLRKERLIELRSGVAILLQPDVLAEVAHYE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCC
PGPAVRAPSPARRQLEKTSA
CCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]