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The map label for this gene is folE [H]

Identifier: 197103883

GI number: 197103883

Start: 423570

End: 424199

Strand: Reverse

Name: folE [H]

Synonym: PHZ_c0417

Alternate gene names: 197103883

Gene position: 424199-423570 (Counterclockwise)

Preceding gene: 197103891

Following gene: 197103882

Centisome position: 10.61

GC content: 67.62

Gene sequence:

>630_bases
ATGGATGCTCCTGCTCGCGACCGCACCCTGATCGGGTCCGACGTCGACCTCGAACCCGTGAAGCGCCCGACGCGCGAGGA
GGCCATGGAGGCCGTCCGCACGCTCATCGCCTGGGCCGGCGACGATCCGCGCCGCGAAGGCGTGATCGACACGCCCAAGC
GCGTGGTGGACGCCTACGCCGAATGGTTCGAGGGCTACAGCCTCGATCCGGCCAAGGAGTTGTCGCGCACCTTCGAGGAC
GTCCAGGGCTACGACGACATCGTCATGCTCCGCGACATCGAGGTGGAAAGCCACTGCGAGCACCACATGGCCCCGTTCCT
CGGCAAGGCCTACGTCGCCTACATGCCGACGAACCGCGTGGTGGGCATCTCCAAGCTGGCCAAGGTGGTCGAGATCTTCG
CCCGCCGTCTCCAGACCCAGGAGACCATGACCCAGCAGATCGCCGACGCCATCACCGACAGCCTGCGCCCCGCCGGCGTC
GCCGTGCTGATCGACGCGGCGCACCAGTGCATGACCACGCGCGGCGTGCACCACCGCCACGTCTCGACGATCACCACCCA
GTTCACCGGGGTCTTCAAGACTGACCCCACCCTGCGCCAGCGCTTCCTCGACTTCGTCAACCGCCGCTGA

Upstream 100 bases:

>100_bases
ACGGGAACTCCTGACCCGTACCTTCATCCAACACAGGGTGGCACGCGTCATCTCCGGGACCTTCGTCCCGGCGCAACTCA
GCCCTTGGGATACCTTCTGG

Downstream 100 bases:

>100_bases
CGCCCCCTCCTCTCCCCCTCTCCTCTCCCCCTAGCGCGCAGCGCCGAGAGGGAGAGGATTGAAGAGAGGGAGGGAGAGGA
TTGAGGAGAGGGCCCGCGCG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MDAPARDRTLIGSDVDLEPVKRPTREEAMEAVRTLIAWAGDDPRREGVIDTPKRVVDAYAEWFEGYSLDPAKELSRTFED
VQGYDDIVMLRDIEVESHCEHHMAPFLGKAYVAYMPTNRVVGISKLAKVVEIFARRLQTQETMTQQIADAITDSLRPAGV
AVLIDAAHQCMTTRGVHHRHVSTITTQFTGVFKTDPTLRQRFLDFVNRR

Sequences:

>Translated_209_residues
MDAPARDRTLIGSDVDLEPVKRPTREEAMEAVRTLIAWAGDDPRREGVIDTPKRVVDAYAEWFEGYSLDPAKELSRTFED
VQGYDDIVMLRDIEVESHCEHHMAPFLGKAYVAYMPTNRVVGISKLAKVVEIFARRLQTQETMTQQIADAITDSLRPAGV
AVLIDAAHQCMTTRGVHHRHVSTITTQFTGVFKTDPTLRQRFLDFVNRR
>Mature_209_residues
MDAPARDRTLIGSDVDLEPVKRPTREEAMEAVRTLIAWAGDDPRREGVIDTPKRVVDAYAEWFEGYSLDPAKELSRTFED
VQGYDDIVMLRDIEVESHCEHHMAPFLGKAYVAYMPTNRVVGISKLAKVVEIFARRLQTQETMTQQIADAITDSLRPAGV
AVLIDAAHQCMTTRGVHHRHVSTITTQFTGVFKTDPTLRQRFLDFVNRR

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=194, Percent_Identity=40.7216494845361, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI4503949, Length=194, Percent_Identity=40.7216494845361, Blast_Score=167, Evalue=8e-42,
Organism=Homo sapiens, GI66932970, Length=164, Percent_Identity=39.0243902439024, Blast_Score=130, Evalue=6e-31,
Organism=Homo sapiens, GI66932972, Length=154, Percent_Identity=40.9090909090909, Blast_Score=128, Evalue=4e-30,
Organism=Escherichia coli, GI1788476, Length=196, Percent_Identity=36.734693877551, Blast_Score=112, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17560486, Length=179, Percent_Identity=43.0167597765363, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6321706, Length=179, Percent_Identity=41.340782122905, Blast_Score=166, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24656791, Length=217, Percent_Identity=39.63133640553, Blast_Score=163, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24656782, Length=200, Percent_Identity=41.5, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24656796, Length=219, Percent_Identity=39.2694063926941, Blast_Score=162, Evalue=1e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020588
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 23713; Mature: 23713

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAPARDRTLIGSDVDLEPVKRPTREEAMEAVRTLIAWAGDDPRREGVIDTPKRVVDAYA
CCCCCCCCEECCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
EWFEGYSLDPAKELSRTFEDVQGYDDIVMLRDIEVESHCEHHMAPFLGKAYVAYMPTNRV
HHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHEECCCCCC
VGISKLAKVVEIFARRLQTQETMTQQIADAITDSLRPAGVAVLIDAAHQCMTTRGVHHRH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHH
VSTITTQFTGVFKTDPTLRQRFLDFVNRR
HHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDAPARDRTLIGSDVDLEPVKRPTREEAMEAVRTLIAWAGDDPRREGVIDTPKRVVDAYA
CCCCCCCCEECCCCCCCHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
EWFEGYSLDPAKELSRTFEDVQGYDDIVMLRDIEVESHCEHHMAPFLGKAYVAYMPTNRV
HHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHEECCCCCC
VGISKLAKVVEIFARRLQTQETMTQQIADAITDSLRPAGVAVLIDAAHQCMTTRGVHHRH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHH
VSTITTQFTGVFKTDPTLRQRFLDFVNRR
HHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11259647 [H]