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The map label for this gene is pcbC [H]

Identifier: 197103783

GI number: 197103783

Start: 309644

End: 310597

Strand: Reverse

Name: pcbC [H]

Synonym: PHZ_c0317

Alternate gene names: 197103783

Gene position: 310597-309644 (Counterclockwise)

Preceding gene: 197103788

Following gene: 197103778

Centisome position: 7.77

GC content: 69.6

Gene sequence:

>954_bases
ATGAGCCAAGCCGCCGCCCTGCCCGAGACCATCGAGCCCGTCTCCTACGCCCTGTTCGACCAGGATTTCGAGGCCTTCAC
CCGCAAGCTGGGCGACAGCTTCCGGCGCTGGGGCTTCGCGGTGATCTGCGACCACGGCCTGCCGCAGGAGAAGGTCGACG
CCGCGATCGGCGAGACCAAGGCCTTCTTCGCCCTGCCGGAGGAGACCAAGCTGAAGTACAAGCTGCCGGTGGCCGGCCAG
CGCGGCTACACCCCCTTCGGCGTCGAGACCGCGAAGGGCCACCAGCACTACGACCTGAAGGAGTTCTGGCACGTCGGCCG
CGACCTGCCGCCCGGCCACCGGTTCCGCAGCCACATGCCCGACAACGTCTGGCCCGACGCCGAGCTGCCGCAGTTCCGCC
AGCGCGTGGGCTGGCTGTACGGCGCGCTCGACGCGATGGGGCTGAAGGTGCTGGAGGCGATCGCGACCTACGTCGGCCTC
GAGCGGCGGTTCTTCGAGCCGACGGTGGACTTCGGCAACTCGATCCTGCGGCTGCTGCACTATCCGCCGGTGCCCAAGGA
CGGGCCGCACATCCGGGCCGGCGCCCACGAGGACATCAACGTCATCACCCTGCTGCTGGGCGCCGAGGAGGCGGGGCTGG
AGGTGAAGGACCACGACGGCCGCTGGATCCCGATCAACCCGCCGCCGGGCGCCCTCGTCATCAACATCGGCGACATGCTG
CAGCGGCTGACCAACCACCGCCTGCCGTCCACGACCCACCGGGTGGTCAACCCGGCGCCCGAGCGCCGTGGCTTTCCGCG
CTACTCGACGCCGTTCTTCCTGCACTTCAACTCGGACTACCTGATCCGCACCCTGCCCGGCTGCGTCGACGCCGCCCACC
CGGACCGCTACCCCGAACCGATCACGGCCGACGACTACCTCCAGGAACGCCTGCGCGAGATCAAGCTGGCGTAG

Upstream 100 bases:

>100_bases
ATGTGGCGGCTAACGCCCCCGGGAGGCAACACGCGAGCGGGCGAAGGGCTGATCGCCGCGCCCGCTGGAGGCGCGCGGCG
GCCGGGTGTAGAGGAGGGCC

Downstream 100 bases:

>100_bases
CTGTTCCTTCTCCCCTTGCGGGAGAAGGCGGCCGGCGGAGCCGGTCGGATGAGGGGTTTCCCGGGCCCCCGACAAATGAC
GAAGGGGCCGCGCCCGCGGT

Product: oxidoreductase iron/ascorbate family

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSQAAALPETIEPVSYALFDQDFEAFTRKLGDSFRRWGFAVICDHGLPQEKVDAAIGETKAFFALPEETKLKYKLPVAGQ
RGYTPFGVETAKGHQHYDLKEFWHVGRDLPPGHRFRSHMPDNVWPDAELPQFRQRVGWLYGALDAMGLKVLEAIATYVGL
ERRFFEPTVDFGNSILRLLHYPPVPKDGPHIRAGAHEDINVITLLLGAEEAGLEVKDHDGRWIPINPPPGALVINIGDML
QRLTNHRLPSTTHRVVNPAPERRGFPRYSTPFFLHFNSDYLIRTLPGCVDAAHPDRYPEPITADDYLQERLREIKLA

Sequences:

>Translated_317_residues
MSQAAALPETIEPVSYALFDQDFEAFTRKLGDSFRRWGFAVICDHGLPQEKVDAAIGETKAFFALPEETKLKYKLPVAGQ
RGYTPFGVETAKGHQHYDLKEFWHVGRDLPPGHRFRSHMPDNVWPDAELPQFRQRVGWLYGALDAMGLKVLEAIATYVGL
ERRFFEPTVDFGNSILRLLHYPPVPKDGPHIRAGAHEDINVITLLLGAEEAGLEVKDHDGRWIPINPPPGALVINIGDML
QRLTNHRLPSTTHRVVNPAPERRGFPRYSTPFFLHFNSDYLIRTLPGCVDAAHPDRYPEPITADDYLQERLREIKLA
>Mature_316_residues
SQAAALPETIEPVSYALFDQDFEAFTRKLGDSFRRWGFAVICDHGLPQEKVDAAIGETKAFFALPEETKLKYKLPVAGQR
GYTPFGVETAKGHQHYDLKEFWHVGRDLPPGHRFRSHMPDNVWPDAELPQFRQRVGWLYGALDAMGLKVLEAIATYVGLE
RRFFEPTVDFGNSILRLLHYPPVPKDGPHIRAGAHEDINVITLLLGAEEAGLEVKDHDGRWIPINPPPGALVINIGDMLQ
RLTNHRLPSTTHRVVNPAPERRGFPRYSTPFFLHFNSDYLIRTLPGCVDAAHPDRYPEPITADDYLQERLREIKLA

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI28572124, Length=269, Percent_Identity=28.996282527881, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI85816264, Length=281, Percent_Identity=28.1138790035587, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 35946; Mature: 35815

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAAALPETIEPVSYALFDQDFEAFTRKLGDSFRRWGFAVICDHGLPQEKVDAAIGETK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCC
AFFALPEETKLKYKLPVAGQRGYTPFGVETAKGHQHYDLKEFWHVGRDLPPGHRFRSHMP
EEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHCCC
DNVWPDAELPQFRQRVGWLYGALDAMGLKVLEAIATYVGLERRFFEPTVDFGNSILRLLH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YPPVPKDGPHIRAGAHEDINVITLLLGAEEAGLEVKDHDGRWIPINPPPGALVINIGDML
CCCCCCCCCCCCCCCCCCCEEEEEEECCHHHCCEEECCCCCEEECCCCCCEEEEEHHHHH
QRLTNHRLPSTTHRVVNPAPERRGFPRYSTPFFLHFNSDYLIRTLPGCVDAAHPDRYPEP
HHHHHCCCCCCHHHHCCCCHHHCCCCCCCCCEEEEECCCCHHHHCCCHHCCCCCCCCCCC
ITADDYLQERLREIKLA
CCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQAAALPETIEPVSYALFDQDFEAFTRKLGDSFRRWGFAVICDHGLPQEKVDAAIGETK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHCCCC
AFFALPEETKLKYKLPVAGQRGYTPFGVETAKGHQHYDLKEFWHVGRDLPPGHRFRSHMP
EEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHCCC
DNVWPDAELPQFRQRVGWLYGALDAMGLKVLEAIATYVGLERRFFEPTVDFGNSILRLLH
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YPPVPKDGPHIRAGAHEDINVITLLLGAEEAGLEVKDHDGRWIPINPPPGALVINIGDML
CCCCCCCCCCCCCCCCCCCEEEEEEECCHHHCCEEECCCCCEEECCCCCCEEEEEHHHHH
QRLTNHRLPSTTHRVVNPAPERRGFPRYSTPFFLHFNSDYLIRTLPGCVDAAHPDRYPEP
HHHHHCCCCCCHHHHCCCCHHHCCCCCCCCCEEEEECCCCHHHHCCCHHCCCCCCCCCCC
ITADDYLQERLREIKLA
CCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1956290 [H]