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The map label for this gene is mscL

Identifier: 197103658

GI number: 197103658

Start: 186121

End: 186540

Strand: Direct

Name: mscL

Synonym: PHZ_c0192

Alternate gene names: 197103658

Gene position: 186121-186540 (Clockwise)

Preceding gene: 197103657

Following gene: 197103660

Centisome position: 4.66

GC content: 65.71

Gene sequence:

>420_bases
ATGAGCATCATCTCGGAATTCCGCGAATTCATCGCCCGCGGCAACGTGATCGACCTGGCGGTCGGCGTGATCATCGGCGC
GGCGTTCAACGACATCGTCAAGGCGCTGGTCGACAACATCGTCATGCCGCCGATCGGGCTCGTCCTGTCGGGCATCGACT
TCTCGGACCTGGCCTGGGTGCTGAAGCCGGACGATCCGGCGACGCCGGCGCTCGACGCCGTGGCGATCCAGTACGGCGCC
TTCATCAACACCTGCATCCGCTTCCTGATCGTCGCCTGGGCGGTGTTCATGCTGGTGAAGCTGGTGAACGTGATCCGCCG
CCGCGAGGCCGAGAAGCCGCCCGAGGAAAAGCCCGCGCCGACGCCGCAGGAAACCCTGCTGATGGAGATCCGCGACCTCC
TGAAGCGGCGGGCGGACTAG

Upstream 100 bases:

>100_bases
CGTGCGGGCCAAGCACGTGCCCTTCTTCAAGAGCGGCAAGGAGCTGCGCGAGCGGCTGAACGCCGTGGACGAGCGCTAAG
CCGCCCCGGAGGGCTGGTCG

Downstream 100 bases:

>100_bases
GGCCTCGCGCGACCGCTGGATCCCCGACAGGCGGCTTCGCCGCCTTCCGGGATGGCGTGACCGGGGTCACGCCACCTTGA
AGCCTTCCCAGTTCATCATC

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 139; Mature: 138

Protein sequence:

>139_residues
MSIISEFREFIARGNVIDLAVGVIIGAAFNDIVKALVDNIVMPPIGLVLSGIDFSDLAWVLKPDDPATPALDAVAIQYGA
FINTCIRFLIVAWAVFMLVKLVNVIRRREAEKPPEEKPAPTPQETLLMEIRDLLKRRAD

Sequences:

>Translated_139_residues
MSIISEFREFIARGNVIDLAVGVIIGAAFNDIVKALVDNIVMPPIGLVLSGIDFSDLAWVLKPDDPATPALDAVAIQYGA
FINTCIRFLIVAWAVFMLVKLVNVIRRREAEKPPEEKPAPTPQETLLMEIRDLLKRRAD
>Mature_138_residues
SIISEFREFIARGNVIDLAVGVIIGAAFNDIVKALVDNIVMPPIGLVLSGIDFSDLAWVLKPDDPATPALDAVAIQYGAF
INTCIRFLIVAWAVFMLVKLVNVIRRREAEKPPEEKPAPTPQETLLMEIRDLLKRRAD

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=139, Percent_Identity=54.6762589928058, Blast_Score=145, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_PHEZH (B4RCK7)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129035.1
- GeneID:   6789682
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0192
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK00567
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15383; Mature: 15252

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1e7b0404)-; HASH(0x1e7c1008)-; HASH(0x1ce99410)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIISEFREFIARGNVIDLAVGVIIGAAFNDIVKALVDNIVMPPIGLVLSGIDFSDLAWV
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHEEE
LKPDDPATPALDAVAIQYGAFINTCIRFLIVAWAVFMLVKLVNVIRRREAEKPPEEKPAP
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TPQETLLMEIRDLLKRRAD
CHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
SIISEFREFIARGNVIDLAVGVIIGAAFNDIVKALVDNIVMPPIGLVLSGIDFSDLAWV
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHEEE
LKPDDPATPALDAVAIQYGAFINTCIRFLIVAWAVFMLVKLVNVIRRREAEKPPEEKPAP
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
TPQETLLMEIRDLLKRRAD
CHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA