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The map label for this gene is gltA [H]

Identifier: 197103628

GI number: 197103628

Start: 153528

End: 154598

Strand: Reverse

Name: gltA [H]

Synonym: PHZ_c0162

Alternate gene names: 197103628

Gene position: 154598-153528 (Counterclockwise)

Preceding gene: 197103630

Following gene: 197103622

Centisome position: 3.87

GC content: 75.35

Gene sequence:

>1071_bases
ATGTCGGACGGACTTGAAGGCGTGGTCGCGGCGCGCACCGTGCTGTCGGAGGTGGACGGCCAAGCCGGCCGGCTGATCAT
CCGCGGCCGCTCGCTGGACGAGCTGGCGGGGCGGACGCGGTTCGAGGATCTGATCGCCCTGCTGTTCGAGGGCTTCTACG
ACCTGCCGGCCGACCTGGCCGGCGCCCTGGGCTGGGCGCGGTCGGAGGTGTTCGCCGAGGTGGCGGCCCTCGACACCGGT
CTCCTCGACCGCACGCCGGTCGAGGCCGTGCGGGCGCTCATGGCCCGGCTGCCGGACGGCGACGACACCGCCACCGCGCT
GCGGCTGCTGGCGGCGCCTTCGGTGTTCACCGCCGCGGTGGTGCGCGCGCAGGCCGGAGAGGCGCCCGTGGAGCCCGACC
CGGGCCTGTCCCATGCGGCCGATACGCTGCGGATGCTGCGCGGCGCGGCGCCGTCGAAGGCCGAGGCCGACGCGCTGGAC
GCCTATCTCGTGACCGTCAGCGACCACGGGCTGAACGCCTCCACCTTCGCCGCGCGGGTGGTGGCCTCGACCCGCGCCGG
CCTGACCAGCGCGGTCCTGGCCGGGATCAGCGCCCTGAAGGGCCCGCTGCACGGCGGCGCGCCGGGGCCGGTGATCGAGA
TGCTGGACGCCATCGGCGAGCCCTCGAACGCCCGCGCCTGGATCGAGGCGGCGCTCGGCCGGGGCGAGCGGCTGATGGGC
TTCGGCCACCGGATCTATCGGGTGCGCGACCCGCGGGCCGATGCGCTGAAGCGGGCGGTGAAGTGGCTGGACGCCCGGCA
GGGGCGGCTGGAGTTCGCCGAGGCGGTCGAGGCGGCGGCGCTCGCGATCCTGCGCGAGAAGAAGCCGGACCGGCCGCTGG
ACACCAACGTCGAGTTCTACACCGCCCTCCTGCTGGAGGGGCTCGGCTTCCCGCCGGCGGCCTTCACCTGCGTCTTCGCC
ATGGGCCGGACGGCCGGCTGGCTGGCCCACGCCCGCGAACAGCTGGCCGGGGGCCGGCTGATCCGCCCGCAGTCGGTGTA
CGTGGGTCCCGCGCCAATGCGGGCGGCTTAG

Upstream 100 bases:

>100_bases
TCCAGTCGCGCTGAGGCATTTATATTGATCATATTGATCAAGATTGACGACAGGAGCCTGGAGGCAAATCTCCCGTCCGA
CAAGAGCAATGGAGGCCGAT

Downstream 100 bases:

>100_bases
GATGAAGCAGGGCTGATGAGAGGCTGAGCCGGCCCTTCAGGACGAAAGAGGGGCCGCACTCGCGATGCGGCCCCTCATCC
GTCGTTCTTCCGGCGACACC

Product: citrate synthase 2

Products: NA

Alternate protein names: Citrate synthase I [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MSDGLEGVVAARTVLSEVDGQAGRLIIRGRSLDELAGRTRFEDLIALLFEGFYDLPADLAGALGWARSEVFAEVAALDTG
LLDRTPVEAVRALMARLPDGDDTATALRLLAAPSVFTAAVVRAQAGEAPVEPDPGLSHAADTLRMLRGAAPSKAEADALD
AYLVTVSDHGLNASTFAARVVASTRAGLTSAVLAGISALKGPLHGGAPGPVIEMLDAIGEPSNARAWIEAALGRGERLMG
FGHRIYRVRDPRADALKRAVKWLDARQGRLEFAEAVEAAALAILREKKPDRPLDTNVEFYTALLLEGLGFPPAAFTCVFA
MGRTAGWLAHAREQLAGGRLIRPQSVYVGPAPMRAA

Sequences:

>Translated_356_residues
MSDGLEGVVAARTVLSEVDGQAGRLIIRGRSLDELAGRTRFEDLIALLFEGFYDLPADLAGALGWARSEVFAEVAALDTG
LLDRTPVEAVRALMARLPDGDDTATALRLLAAPSVFTAAVVRAQAGEAPVEPDPGLSHAADTLRMLRGAAPSKAEADALD
AYLVTVSDHGLNASTFAARVVASTRAGLTSAVLAGISALKGPLHGGAPGPVIEMLDAIGEPSNARAWIEAALGRGERLMG
FGHRIYRVRDPRADALKRAVKWLDARQGRLEFAEAVEAAALAILREKKPDRPLDTNVEFYTALLLEGLGFPPAAFTCVFA
MGRTAGWLAHAREQLAGGRLIRPQSVYVGPAPMRAA
>Mature_355_residues
SDGLEGVVAARTVLSEVDGQAGRLIIRGRSLDELAGRTRFEDLIALLFEGFYDLPADLAGALGWARSEVFAEVAALDTGL
LDRTPVEAVRALMARLPDGDDTATALRLLAAPSVFTAAVVRAQAGEAPVEPDPGLSHAADTLRMLRGAAPSKAEADALDA
YLVTVSDHGLNASTFAARVVASTRAGLTSAVLAGISALKGPLHGGAPGPVIEMLDAIGEPSNARAWIEAALGRGERLMGF
GHRIYRVRDPRADALKRAVKWLDARQGRLEFAEAVEAAALAILREKKPDRPLDTNVEFYTALLLEGLGFPPAAFTCVFAM
GRTAGWLAHAREQLAGGRLIRPQSVYVGPAPMRAA

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786939, Length=376, Percent_Identity=29.5212765957447, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786527, Length=365, Percent_Identity=30.1369863013699, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319850, Length=251, Percent_Identity=25.4980079681275, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 37514; Mature: 37383

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGLEGVVAARTVLSEVDGQAGRLIIRGRSLDELAGRTRFEDLIALLFEGFYDLPADLA
CCCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GALGWARSEVFAEVAALDTGLLDRTPVEAVRALMARLPDGDDTATALRLLAAPSVFTAAV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
VRAQAGEAPVEPDPGLSHAADTLRMLRGAAPSKAEADALDAYLVTVSDHGLNASTFAARV
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHEEEEEEECCCCCHHHHHHHH
VASTRAGLTSAVLAGISALKGPLHGGAPGPVIEMLDAIGEPSNARAWIEAALGRGERLMG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
FGHRIYRVRDPRADALKRAVKWLDARQGRLEFAEAVEAAALAILREKKPDRPLDTNVEFY
CCHHEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
TALLLEGLGFPPAAFTCVFAMGRTAGWLAHAREQLAGGRLIRPQSVYVGPAPMRAA
HHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCEECCCCEEECCCCCCCC
>Mature Secondary Structure 
SDGLEGVVAARTVLSEVDGQAGRLIIRGRSLDELAGRTRFEDLIALLFEGFYDLPADLA
CCCHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GALGWARSEVFAEVAALDTGLLDRTPVEAVRALMARLPDGDDTATALRLLAAPSVFTAAV
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHH
VRAQAGEAPVEPDPGLSHAADTLRMLRGAAPSKAEADALDAYLVTVSDHGLNASTFAARV
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHEEEEEEECCCCCHHHHHHHH
VASTRAGLTSAVLAGISALKGPLHGGAPGPVIEMLDAIGEPSNARAWIEAALGRGERLMG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHH
FGHRIYRVRDPRADALKRAVKWLDARQGRLEFAEAVEAAALAILREKKPDRPLDTNVEFY
CCHHEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHH
TALLLEGLGFPPAAFTCVFAMGRTAGWLAHAREQLAGGRLIRPQSVYVGPAPMRAA
HHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCEECCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045898; 9579061; 9384377 [H]