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The map label for this gene is pycB [H]

Identifier: 195953006

GI number: 195953006

Start: 563496

End: 565355

Strand: Direct

Name: pycB [H]

Synonym: HY04AAS1_0631

Alternate gene names: 195953006

Gene position: 563496-565355 (Clockwise)

Preceding gene: 195953004

Following gene: 195953007

Centisome position: 36.13

GC content: 36.13

Gene sequence:

>1860_bases
ATGGAGAAATTATATTTTACCGATGTTAGCCTAAGAGACGGTATCCAGAGTCTTTTAGCCACGAGAGTACGAACAGAGGA
TATGCTTCCTGCGTTAGAAATTTTGGATAAATGCGGATTTTGGTCTTTGGAAGTTTGGGGCGGCGCTACATTTGATGTAT
GTCTTAGGTACCTAAAAGAAGATCCATGGGAACGTTTAAAACAATTTAAAAACATCACAAAAAATACTAAACTTGAAATG
TTGTTAAGAGGTCAAAATATCGTAGGCTATAGGAATTATCCAGACGACGTTGTGGAGGCTTTTGTTAAAAAATCCTACGA
CAATGGAGTAGAAGTTTTTAGAATTTTTGACGCATTAAACGATGTTAGAAACATGAGAAAAGCCATAGAAACCGGCAAAA
GCTTAAATGCTATAGTAAAAGGGGTATTATCGTATACAACAAGCCCAGTCCATACCGTCGAATACTACGTAAAAATAGCA
AAAGATTTAGTAAATATGGGTATAGATATAATTTCTATAAAAGACCAATCTGGCATACTAACTCCAAAAATGGCTTTTGA
TTTGGTAAAAGCTCTAAAACAAGAATTTAAGCTTCCTATACATATACATACGCAGACCACTGCAGAACTTGCAAATATGT
CTCAGCTTAAAGCTTTAGAAGCTGGAGCAACTATGTTTGATACGGCTTTTTATAGCTTATCATCTCAGACTTCCCATCCA
TCTGGAGAAACAATGATACATGTTTTTAAGGAATTTGGCTATCAAATAGATGTAGACCAAGCACTCTATGAAGAAGCTGG
CAATGTTTTTAGAGAGGTTAGAAAAAAGTATAAAAAATACGACACACTGCCTCCTTATCCAGACGTATCCGTATTAACAC
ACCAAGTACCTGGTGGTATGATTAGCAACTTTATAAGCCAGCTTAAAGAACAAAATTTAGAAGATAAATTGGAAGAAGTG
TTGAAAGAGGTAGCTAGAGTTAGGGAAGACTTAGGGTATCCTCCTCTTGTGACGCCCACAAGCCAAATGGTGGGGTCTCA
AGCTTTTCTAAATATAGTAAATAAAGAAAGATATAAGGTTGTCACTAAAGAAATAAAAGACTATGTGAAAGGTTTATATG
GAAAACCACCAGCCCCTATCAAACCGGAAATTATGGAATTGATATTAAAAGACGAAAAAGATAAAGTCTATTTTGATATA
AGACCAGCCGATCTTCTTCAAAACGAGTTAGATGAAATAAGAAAAGAAGCCTACGACGCAGGTGCAAGAAACGAAGAAGA
CGTACTTTCTTATGCTATATTACCAGTAGTAGCTAAAGAGTTTTTTGAATGGCGAGAAAAGTTTGAAAAAGGTTTGGTGC
AGAAAGAAATAGAGTATACTTATAGCGATTGCAACGAAAATGTACCCGTGGAATTTAACGTAGCCGTACACGGTGAGCAG
TTTAACATAAAAATAGTTGGCAGAGAAGGGATGTCAAAAGATAGAAAGCTCTTCTTCATAAGAATAAACGATCAATTAGA
AGAGGTTACCATTGAGCCGATTAGAGAAATAACAGTTTCCCAACAAAAGTCCAGCAGCGTTGATATACCAGAAGGGACAT
CTGTAAAACCAAAAAGACCAAAAGCTCAAGACATAGGTGATATATCTTCAAACATTTCAGGTAAGGTTGTGGATATAAAG
GTAAGTATAGGCGATAAGGTAAAAGAAGGTGATACGCTTTTAATAGTAGAAGCCATGAAGATGGAAAATGAGATAAAAAG
CACCATAGACGGACAAGTAGAGGAAATTCTGGTAGAAATAGGAGAGGTTATAAATCCCGGTGAAGTGCTCATAAGGCTAA
AACCAGAATTTGATGCTTAA

Upstream 100 bases:

>100_bases
TTCATGGTGTAATGTAATAAAATCCAAAAATTTTTCCCAATAGGTCTTTAGCTTTTCAACCATAAAAAATTATTTTACCA
TTTTAGTGTATAATAGTGAT

Downstream 100 bases:

>100_bases
AATAGCAATACCAAAAGGAAGACTGCTAGAAGAAGTATCGGAATTATTTCTTAGTAAAAGCCTGATACCTAGCAAAATTG
AAGAAAGCAGAAAGCTGATA

Product: pyruvate carboxylase subunit B

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MEKLYFTDVSLRDGIQSLLATRVRTEDMLPALEILDKCGFWSLEVWGGATFDVCLRYLKEDPWERLKQFKNITKNTKLEM
LLRGQNIVGYRNYPDDVVEAFVKKSYDNGVEVFRIFDALNDVRNMRKAIETGKSLNAIVKGVLSYTTSPVHTVEYYVKIA
KDLVNMGIDIISIKDQSGILTPKMAFDLVKALKQEFKLPIHIHTQTTAELANMSQLKALEAGATMFDTAFYSLSSQTSHP
SGETMIHVFKEFGYQIDVDQALYEEAGNVFREVRKKYKKYDTLPPYPDVSVLTHQVPGGMISNFISQLKEQNLEDKLEEV
LKEVARVREDLGYPPLVTPTSQMVGSQAFLNIVNKERYKVVTKEIKDYVKGLYGKPPAPIKPEIMELILKDEKDKVYFDI
RPADLLQNELDEIRKEAYDAGARNEEDVLSYAILPVVAKEFFEWREKFEKGLVQKEIEYTYSDCNENVPVEFNVAVHGEQ
FNIKIVGREGMSKDRKLFFIRINDQLEEVTIEPIREITVSQQKSSSVDIPEGTSVKPKRPKAQDIGDISSNISGKVVDIK
VSIGDKVKEGDTLLIVEAMKMENEIKSTIDGQVEEILVEIGEVINPGEVLIRLKPEFDA

Sequences:

>Translated_619_residues
MEKLYFTDVSLRDGIQSLLATRVRTEDMLPALEILDKCGFWSLEVWGGATFDVCLRYLKEDPWERLKQFKNITKNTKLEM
LLRGQNIVGYRNYPDDVVEAFVKKSYDNGVEVFRIFDALNDVRNMRKAIETGKSLNAIVKGVLSYTTSPVHTVEYYVKIA
KDLVNMGIDIISIKDQSGILTPKMAFDLVKALKQEFKLPIHIHTQTTAELANMSQLKALEAGATMFDTAFYSLSSQTSHP
SGETMIHVFKEFGYQIDVDQALYEEAGNVFREVRKKYKKYDTLPPYPDVSVLTHQVPGGMISNFISQLKEQNLEDKLEEV
LKEVARVREDLGYPPLVTPTSQMVGSQAFLNIVNKERYKVVTKEIKDYVKGLYGKPPAPIKPEIMELILKDEKDKVYFDI
RPADLLQNELDEIRKEAYDAGARNEEDVLSYAILPVVAKEFFEWREKFEKGLVQKEIEYTYSDCNENVPVEFNVAVHGEQ
FNIKIVGREGMSKDRKLFFIRINDQLEEVTIEPIREITVSQQKSSSVDIPEGTSVKPKRPKAQDIGDISSNISGKVVDIK
VSIGDKVKEGDTLLIVEAMKMENEIKSTIDGQVEEILVEIGEVINPGEVLIRLKPEFDA
>Mature_619_residues
MEKLYFTDVSLRDGIQSLLATRVRTEDMLPALEILDKCGFWSLEVWGGATFDVCLRYLKEDPWERLKQFKNITKNTKLEM
LLRGQNIVGYRNYPDDVVEAFVKKSYDNGVEVFRIFDALNDVRNMRKAIETGKSLNAIVKGVLSYTTSPVHTVEYYVKIA
KDLVNMGIDIISIKDQSGILTPKMAFDLVKALKQEFKLPIHIHTQTTAELANMSQLKALEAGATMFDTAFYSLSSQTSHP
SGETMIHVFKEFGYQIDVDQALYEEAGNVFREVRKKYKKYDTLPPYPDVSVLTHQVPGGMISNFISQLKEQNLEDKLEEV
LKEVARVREDLGYPPLVTPTSQMVGSQAFLNIVNKERYKVVTKEIKDYVKGLYGKPPAPIKPEIMELILKDEKDKVYFDI
RPADLLQNELDEIRKEAYDAGARNEEDVLSYAILPVVAKEFFEWREKFEKGLVQKEIEYTYSDCNENVPVEFNVAVHGEQ
FNIKIVGREGMSKDRKLFFIRINDQLEEVTIEPIREITVSQQKSSSVDIPEGTSVKPKRPKAQDIGDISSNISGKVVDIK
VSIGDKVKEGDTLLIVEAMKMENEIKSTIDGQVEEILVEIGEVINPGEVLIRLKPEFDA

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=630, Percent_Identity=30.6349206349206, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI106049295, Length=630, Percent_Identity=30.6349206349206, Blast_Score=264, Evalue=2e-70,
Organism=Homo sapiens, GI106049292, Length=630, Percent_Identity=30.6349206349206, Blast_Score=264, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17562816, Length=641, Percent_Identity=31.98127925117, Blast_Score=273, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6319695, Length=637, Percent_Identity=29.8273155416013, Blast_Score=285, Evalue=2e-77,
Organism=Saccharomyces cerevisiae, GI6321376, Length=634, Percent_Identity=30.7570977917981, Blast_Score=282, Evalue=1e-76,
Organism=Drosophila melanogaster, GI24652212, Length=643, Percent_Identity=31.7262830482115, Blast_Score=272, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24652210, Length=643, Percent_Identity=31.7262830482115, Blast_Score=272, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24652214, Length=643, Percent_Identity=31.7262830482115, Blast_Score=272, Evalue=6e-73,
Organism=Drosophila melanogaster, GI19921944, Length=643, Percent_Identity=31.7262830482115, Blast_Score=272, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24652216, Length=643, Percent_Identity=31.7262830482115, Blast_Score=272, Evalue=6e-73,
Organism=Drosophila melanogaster, GI281363050, Length=643, Percent_Identity=31.7262830482115, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24652224, Length=643, Percent_Identity=31.7262830482115, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24652222, Length=643, Percent_Identity=31.7262830482115, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24652220, Length=643, Percent_Identity=31.7262830482115, Blast_Score=271, Evalue=8e-73,
Organism=Drosophila melanogaster, GI24652218, Length=643, Percent_Identity=31.7262830482115, Blast_Score=271, Evalue=8e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 70447; Mature: 70447

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLYFTDVSLRDGIQSLLATRVRTEDMLPALEILDKCGFWSLEVWGGATFDVCLRYLKE
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHC
DPWERLKQFKNITKNTKLEMLLRGQNIVGYRNYPDDVVEAFVKKSYDNGVEVFRIFDALN
CHHHHHHHHHHHHCCCCEEEEEECCCEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
DVRNMRKAIETGKSLNAIVKGVLSYTTSPVHTVEYYVKIAKDLVNMGIDIISIKDQSGIL
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
TPKMAFDLVKALKQEFKLPIHIHTQTTAELANMSQLKALEAGATMFDTAFYSLSSQTSHP
CHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
SGETMIHVFKEFGYQIDVDQALYEEAGNVFREVRKKYKKYDTLPPYPDVSVLTHQVPGGM
CCHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHH
ISNFISQLKEQNLEDKLEEVLKEVARVREDLGYPPLVTPTSQMVGSQAFLNIVNKERYKV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VTKEIKDYVKGLYGKPPAPIKPEIMELILKDEKDKVYFDIRPADLLQNELDEIRKEAYDA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCC
GARNEEDVLSYAILPVVAKEFFEWREKFEKGLVQKEIEYTYSDCNENVPVEFNVAVHGEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCE
FNIKIVGREGMSKDRKLFFIRINDQLEEVTIEPIREITVSQQKSSSVDIPEGTSVKPKRP
EEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KAQDIGDISSNISGKVVDIKVSIGDKVKEGDTLLIVEAMKMENEIKSTIDGQVEEILVEI
CCHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCHHHHHHHHHH
GEVINPGEVLIRLKPEFDA
HHCCCCCCEEEEECCCCCC
>Mature Secondary Structure
MEKLYFTDVSLRDGIQSLLATRVRTEDMLPALEILDKCGFWSLEVWGGATFDVCLRYLKE
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHC
DPWERLKQFKNITKNTKLEMLLRGQNIVGYRNYPDDVVEAFVKKSYDNGVEVFRIFDALN
CHHHHHHHHHHHHCCCCEEEEEECCCEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
DVRNMRKAIETGKSLNAIVKGVLSYTTSPVHTVEYYVKIAKDLVNMGIDIISIKDQSGIL
HHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCC
TPKMAFDLVKALKQEFKLPIHIHTQTTAELANMSQLKALEAGATMFDTAFYSLSSQTSHP
CHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
SGETMIHVFKEFGYQIDVDQALYEEAGNVFREVRKKYKKYDTLPPYPDVSVLTHQVPGGM
CCHHHHHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCHH
ISNFISQLKEQNLEDKLEEVLKEVARVREDLGYPPLVTPTSQMVGSQAFLNIVNKERYKV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VTKEIKDYVKGLYGKPPAPIKPEIMELILKDEKDKVYFDIRPADLLQNELDEIRKEAYDA
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCC
GARNEEDVLSYAILPVVAKEFFEWREKFEKGLVQKEIEYTYSDCNENVPVEFNVAVHGEQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCE
FNIKIVGREGMSKDRKLFFIRINDQLEEVTIEPIREITVSQQKSSSVDIPEGTSVKPKRP
EEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KAQDIGDISSNISGKVVDIKVSIGDKVKEGDTLLIVEAMKMENEIKSTIDGQVEEILVEI
CCHHHHHHHCCCCCEEEEEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHCHHHHHHHHHH
GEVINPGEVLIRLKPEFDA
HHCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]