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The map label for this gene is bioD

Identifier: 195952892

GI number: 195952892

Start: 454721

End: 455386

Strand: Direct

Name: bioD

Synonym: HY04AAS1_0517

Alternate gene names: 195952892

Gene position: 454721-455386 (Clockwise)

Preceding gene: 195952891

Following gene: 195952893

Centisome position: 29.16

GC content: 31.83

Gene sequence:

>666_bases
ATGATATTGTTTGTAAGCGCCACAGATACAGGTGTTGGTAAGACGTATATTAGCACCATTTTGGTGGAATACTTGAAAAA
TAAAGGCATAAACGTTGATTATATAAAGCCTATAGAAACTGGTGTTGATGAAAAACCAGAAGATGCTTACAAAGTAAGTA
GTATTTTAGGTAAACCATGGCAAGAAAGTGTAATATATATTTTCAAAAAACCAATGTCTCCTTACGCTTGCTCTTTAGAT
GAAGAAGTAGATATAGATGTCGATAGAATCATTAAAACAATAAAAGAAAGAGAACAAAAAGCTAGCGTGCTTATAGTGGA
AGGAGCAGGTGGTATTGCTGTGCCTATAAAGGTAAAACCGCTTATAGATTATGCATATTTAATTAAATCTTTACATGCGT
TGCCTCTTGTTGTAGCAAGAGCTTATCTTGGGACGCTTAATCATAGCTTTTTAACTTATTTTTATCTTAAAAGCAAAGAT
ATAAAACCTCTTGGGTTTGTTTTAAATGGTTTTGATTATGAAGAACCCTCACAATATTCAAATGCCTATATACTTCAAGA
TATGATAGAAGAGCATATTAATATATGGAGGCTAGATAAAAATCCAGACGAAAATCAGAGAATTAACCTTGCAGAAAATA
TCTGGAAAAGTATTGCTAATTTTTAA

Upstream 100 bases:

>100_bases
AAAAAACCTTTTCTGTTAGAAGTAAAAACGAAGAATTCCAAGGTGTTATGGGTATTTATGATTTTATAGAAAAACTCAAA
ACGGCTATTCAAAACAAAAG

Downstream 100 bases:

>100_bases
TTTTATTTTAAATTTATATTTATCACTTCATTTTGGAGGTAAGTGTTTTGCAGACAGCTAAGAATATACTTATTTGGTTT
TTAATTATAGGTTTTATGAT

Product: dethiobiotin synthase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MILFVSATDTGVGKTYISTILVEYLKNKGINVDYIKPIETGVDEKPEDAYKVSSILGKPWQESVIYIFKKPMSPYACSLD
EEVDIDVDRIIKTIKEREQKASVLIVEGAGGIAVPIKVKPLIDYAYLIKSLHALPLVVARAYLGTLNHSFLTYFYLKSKD
IKPLGFVLNGFDYEEPSQYSNAYILQDMIEEHINIWRLDKNPDENQRINLAENIWKSIANF

Sequences:

>Translated_221_residues
MILFVSATDTGVGKTYISTILVEYLKNKGINVDYIKPIETGVDEKPEDAYKVSSILGKPWQESVIYIFKKPMSPYACSLD
EEVDIDVDRIIKTIKEREQKASVLIVEGAGGIAVPIKVKPLIDYAYLIKSLHALPLVVARAYLGTLNHSFLTYFYLKSKD
IKPLGFVLNGFDYEEPSQYSNAYILQDMIEEHINIWRLDKNPDENQRINLAENIWKSIANF
>Mature_221_residues
MILFVSATDTGVGKTYISTILVEYLKNKGINVDYIKPIETGVDEKPEDAYKVSSILGKPWQESVIYIFKKPMSPYACSLD
EEVDIDVDRIIKTIKEREQKASVLIVEGAGGIAVPIKVKPLIDYAYLIKSLHALPLVVARAYLGTLNHSFLTYFYLKSKD
IKPLGFVLNGFDYEEPSQYSNAYILQDMIEEHINIWRLDKNPDENQRINLAENIWKSIANF

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=222, Percent_Identity=28.8288288288288, Blast_Score=71, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324385, Length=182, Percent_Identity=36.8131868131868, Blast_Score=95, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_HYDS0 (B4U7U3)

Other databases:

- EMBL:   CP001130
- RefSeq:   YP_002121182.1
- ProteinModelPortal:   B4U7U3
- SMR:   B4U7U3
- GeneID:   6743313
- GenomeReviews:   CP001130_GR
- KEGG:   hya:HY04AAS1_0517
- HOGENOM:   HBG650065
- OMA:   MLFISAT
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 25156; Mature: 25156

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILFVSATDTGVGKTYISTILVEYLKNKGINVDYIKPIETGVDEKPEDAYKVSSILGKPW
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHCCCCCCCHHHHHHHHHHCCCC
QESVIYIFKKPMSPYACSLDEEVDIDVDRIIKTIKEREQKASVLIVEGAGGIAVPIKVKP
HHCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEHH
LIDYAYLIKSLHALPLVVARAYLGTLNHSFLTYFYLKSKDIKPLGFVLNGFDYEEPSQYS
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCHHHHEECCCCCCCCHHHC
NAYILQDMIEEHINIWRLDKNPDENQRINLAENIWKSIANF
CHHHHHHHHHHHCCEEECCCCCCCCCEECHHHHHHHHHHCC
>Mature Secondary Structure
MILFVSATDTGVGKTYISTILVEYLKNKGINVDYIKPIETGVDEKPEDAYKVSSILGKPW
CEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHCCCCCCCHHHHHHHHHHCCCC
QESVIYIFKKPMSPYACSLDEEVDIDVDRIIKTIKEREQKASVLIVEGAGGIAVPIKVKP
HHCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEHH
LIDYAYLIKSLHALPLVVARAYLGTLNHSFLTYFYLKSKDIKPLGFVLNGFDYEEPSQYS
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHEEEEEEECCCCCHHHHEECCCCCCCCHHHC
NAYILQDMIEEHINIWRLDKNPDENQRINLAENIWKSIANF
CHHHHHHHHHHHCCEEECCCCCCCCCEECHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA