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The map label for this gene is thrS [H]

Identifier: 195952891

GI number: 195952891

Start: 452811

End: 454724

Strand: Direct

Name: thrS [H]

Synonym: HY04AAS1_0516

Alternate gene names: 195952891

Gene position: 452811-454724 (Clockwise)

Preceding gene: 195952890

Following gene: 195952892

Centisome position: 29.04

GC content: 34.8

Gene sequence:

>1914_bases
ATGAAAATAGTATTTAACAATCAAGAATATGATCTGCAAGCAGGAATAGTTGGTATTGAGCTTTTAAAAGCTTTAAACTT
AGATTCAAAAGATATTATAGGAATATCTATAGACGGTATCATCTACGATTTGCAAACACCAATATACCACGGTGGAACGT
TAAAACCCATAACAAAACAAGACAAAGAAAGCCTCGACATATTAAGGCATTCTTTAGCACATATTATGGCACAAGCCCTA
AAAGAAATATATGGAGATGAAAAAGTACATCTTGGAATAGGACCAACCACTGAGGATGGTTTTTTCTACGACGTAGAAGT
AGAAGGCCACCATATAGGCGAAGAAGATTTAAAGATCATAGAAAACAAAATGAAAGAAATAATAAAAAGAGGTTTAGATA
TTAAAAGAAAAGAACTTCCCAGAGAAGAGGCTTTGAAGTTTTTTGAAAATAAAAAAGAAGTTTATAAACTAGATATCATA
AATTCCTTACCAAGTGATGCTAAAATAAGCGTATACGAACAAGGAGAATTTGTGGATTTATGCAGAGGGCCACACATTCC
AAACACAGCATTGGCTGGGGCTTTTAAGCTTAGTTCTATAGCTGGTGCTTATTGGAGAGCAGATGCATCAAAACCAATGC
TACAAAGAATATATGGGATAGCCTTTTGGACGGAAAAAGAACTAGAAGAAAGACTAAAACTTTATGAAGAAGCCAAAAAA
AGAGACCATAGAAGACTTGGGAAAGAACTTGAGCTTTTTACCATAGATGAACAAGTGGGTCCAGGGCTTATAATTTGGCT
TCCAAAAGGTGCCATACTACGACAGGTGGTAGAGGACTATCTTAAAGAAAAGCACAGAGCTTTAAATTATCAGTTTGTAT
ATACACCTCACGTAGGAAAGTCAACGCTTTGGGAAACAAGCGGACATCTTAGCTATTACAAAGAAAATATGTTTCCAGGG
ATGAAGATTGAAGAAGAGTCTTATTATGTAAAACCTATGAATTGTCCGTTTCATATGGCTATATACAAATCAAAAATAAG
AAGCCATAAAGAGCTTCCTATCAAAATAGCAGAGCTCGGCACTGTCTATAGATATGAGATGTCAGGGGTTTTACATGGGC
TGATGAGGGTAAGAGGTTTTACCCAAGATGATGCGCATATATTTTGTAAAGAAGAAGATGTTAAAAGCGTGATAAAAGAA
ACGCTTGAACTAGCTTTTGATGTGTTGAAAGATTTTGGCTTTTATGATTATCAGATATTCATATCCACAAAACCAAAAGA
TGCCATAGGTACAGAAGAAAACTGGAAAATATCCGAAAGTGCTTTAAAAGAATCTTTGGAAGCCCTCGACAAAACATACG
AAATAGATGAAGGTGGTGGTGCTTTTTACGGTCCTAAAATAGATATAAAAATAAAAGATGCTATAGGTAGATTCTGGCAG
TGCTCTACCATACAGTTTGATTTTAATCTTCCGGAGCGTTTTGACCTTACATATGTAGGAGAAGACAACAAAAAACATAG
ACCATACATAATACATAGAGCCTTGCTTGGTTCTATAGAAAGATTTACAGGTGTTTTGATAGAGCATTATGCAGGTCATT
TACCAATCTGGCTTTCTCCTATACAAGCTGCTATAATACCTATAGCCGATAGACATTTAGACTATGCTAAAGAAGTTTTT
GAGCTTTTAAAAGTCCAAAACATAAGAACATATATAGACGATAGACCAGAAAGAATGAATGCAAAAATAAGAGACAACGA
GCTTCAAAAGATACCGATACTTTTAGTGGTTGGGGATAAAGAGGTGCAAGAAAAAACCTTTTCTGTTAGAAGTAAAAACG
AAGAATTCCAAGGTGTTATGGGTATTTATGATTTTATAGAAAAACTCAAAACGGCTATTCAAAACAAAAGATGA

Upstream 100 bases:

>100_bases
GGCAAAAGAGCAAGGGCTTATAAGGTTGGAGGGAAAAGACTACATTGTAAAAGATGGTGATATAATATACTTTAGATTCA
ACGTATAAAGAGGTTCTAGT

Downstream 100 bases:

>100_bases
TATTGTTTGTAAGCGCCACAGATACAGGTGTTGGTAAGACGTATATTAGCACCATTTTGGTGGAATACTTGAAAAATAAA
GGCATAAACGTTGATTATAT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 637; Mature: 637

Protein sequence:

>637_residues
MKIVFNNQEYDLQAGIVGIELLKALNLDSKDIIGISIDGIIYDLQTPIYHGGTLKPITKQDKESLDILRHSLAHIMAQAL
KEIYGDEKVHLGIGPTTEDGFFYDVEVEGHHIGEEDLKIIENKMKEIIKRGLDIKRKELPREEALKFFENKKEVYKLDII
NSLPSDAKISVYEQGEFVDLCRGPHIPNTALAGAFKLSSIAGAYWRADASKPMLQRIYGIAFWTEKELEERLKLYEEAKK
RDHRRLGKELELFTIDEQVGPGLIIWLPKGAILRQVVEDYLKEKHRALNYQFVYTPHVGKSTLWETSGHLSYYKENMFPG
MKIEEESYYVKPMNCPFHMAIYKSKIRSHKELPIKIAELGTVYRYEMSGVLHGLMRVRGFTQDDAHIFCKEEDVKSVIKE
TLELAFDVLKDFGFYDYQIFISTKPKDAIGTEENWKISESALKESLEALDKTYEIDEGGGAFYGPKIDIKIKDAIGRFWQ
CSTIQFDFNLPERFDLTYVGEDNKKHRPYIIHRALLGSIERFTGVLIEHYAGHLPIWLSPIQAAIIPIADRHLDYAKEVF
ELLKVQNIRTYIDDRPERMNAKIRDNELQKIPILLVVGDKEVQEKTFSVRSKNEEFQGVMGIYDFIEKLKTAIQNKR

Sequences:

>Translated_637_residues
MKIVFNNQEYDLQAGIVGIELLKALNLDSKDIIGISIDGIIYDLQTPIYHGGTLKPITKQDKESLDILRHSLAHIMAQAL
KEIYGDEKVHLGIGPTTEDGFFYDVEVEGHHIGEEDLKIIENKMKEIIKRGLDIKRKELPREEALKFFENKKEVYKLDII
NSLPSDAKISVYEQGEFVDLCRGPHIPNTALAGAFKLSSIAGAYWRADASKPMLQRIYGIAFWTEKELEERLKLYEEAKK
RDHRRLGKELELFTIDEQVGPGLIIWLPKGAILRQVVEDYLKEKHRALNYQFVYTPHVGKSTLWETSGHLSYYKENMFPG
MKIEEESYYVKPMNCPFHMAIYKSKIRSHKELPIKIAELGTVYRYEMSGVLHGLMRVRGFTQDDAHIFCKEEDVKSVIKE
TLELAFDVLKDFGFYDYQIFISTKPKDAIGTEENWKISESALKESLEALDKTYEIDEGGGAFYGPKIDIKIKDAIGRFWQ
CSTIQFDFNLPERFDLTYVGEDNKKHRPYIIHRALLGSIERFTGVLIEHYAGHLPIWLSPIQAAIIPIADRHLDYAKEVF
ELLKVQNIRTYIDDRPERMNAKIRDNELQKIPILLVVGDKEVQEKTFSVRSKNEEFQGVMGIYDFIEKLKTAIQNKR
>Mature_637_residues
MKIVFNNQEYDLQAGIVGIELLKALNLDSKDIIGISIDGIIYDLQTPIYHGGTLKPITKQDKESLDILRHSLAHIMAQAL
KEIYGDEKVHLGIGPTTEDGFFYDVEVEGHHIGEEDLKIIENKMKEIIKRGLDIKRKELPREEALKFFENKKEVYKLDII
NSLPSDAKISVYEQGEFVDLCRGPHIPNTALAGAFKLSSIAGAYWRADASKPMLQRIYGIAFWTEKELEERLKLYEEAKK
RDHRRLGKELELFTIDEQVGPGLIIWLPKGAILRQVVEDYLKEKHRALNYQFVYTPHVGKSTLWETSGHLSYYKENMFPG
MKIEEESYYVKPMNCPFHMAIYKSKIRSHKELPIKIAELGTVYRYEMSGVLHGLMRVRGFTQDDAHIFCKEEDVKSVIKE
TLELAFDVLKDFGFYDYQIFISTKPKDAIGTEENWKISESALKESLEALDKTYEIDEGGGAFYGPKIDIKIKDAIGRFWQ
CSTIQFDFNLPERFDLTYVGEDNKKHRPYIIHRALLGSIERFTGVLIEHYAGHLPIWLSPIQAAIIPIADRHLDYAKEVF
ELLKVQNIRTYIDDRPERMNAKIRDNELQKIPILLVVGDKEVQEKTFSVRSKNEEFQGVMGIYDFIEKLKTAIQNKR

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=597, Percent_Identity=39.8659966499162, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI38202255, Length=596, Percent_Identity=40.1006711409396, Blast_Score=455, Evalue=1e-128,
Organism=Homo sapiens, GI20070344, Length=623, Percent_Identity=34.1894060995185, Blast_Score=372, Evalue=1e-103,
Organism=Escherichia coli, GI1788013, Length=609, Percent_Identity=40.5582922824302, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71984184, Length=600, Percent_Identity=38.3333333333333, Blast_Score=437, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI71984192, Length=600, Percent_Identity=38.3333333333333, Blast_Score=437, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6322112, Length=642, Percent_Identity=36.4485981308411, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322654, Length=398, Percent_Identity=37.6884422110553, Blast_Score=254, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24583839, Length=595, Percent_Identity=40.8403361344538, Blast_Score=474, Evalue=1e-134,
Organism=Drosophila melanogaster, GI24583842, Length=595, Percent_Identity=40.8403361344538, Blast_Score=473, Evalue=1e-133,
Organism=Drosophila melanogaster, GI28574141, Length=595, Percent_Identity=40.8403361344538, Blast_Score=473, Evalue=1e-133,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73610; Mature: 73610

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVFNNQEYDLQAGIVGIELLKALNLDSKDIIGISIDGIIYDLQTPIYHGGTLKPITKQ
CEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCEECCCCCCCCCCC
DKESLDILRHSLAHIMAQALKEIYGDEKVHLGIGPTTEDGFFYDVEVEGHHIGEEDLKII
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHH
ENKMKEIIKRGLDIKRKELPREEALKFFENKKEVYKLDIINSLPSDAKISVYEQGEFVDL
HHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCEEEEEEHHCCCCCCEEEEEECCCCHHH
CRGPHIPNTALAGAFKLSSIAGAYWRADASKPMLQRIYGIAFWTEKELEERLKLYEEAKK
HCCCCCCCHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHEECHHHHHHHHHHHHHHHH
RDHRRLGKELELFTIDEQVGPGLIIWLPKGAILRQVVEDYLKEKHRALNYQFVYTPHVGK
HHHHHCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
STLWETSGHLSYYKENMFPGMKIEEESYYVKPMNCPFHMAIYKSKIRSHKELPIKIAELG
CCCCCCCCCHHHHHHHCCCCCEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCC
TVYRYEMSGVLHGLMRVRGFTQDDAHIFCKEEDVKSVIKETLELAFDVLKDFGFYDYQIF
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
ISTKPKDAIGTEENWKISESALKESLEALDKTYEIDEGGGAFYGPKIDIKIKDAIGRFWQ
EECCCCCCCCCCCCCEECHHHHHHHHHHHHHHEEECCCCCEEECCEEEEEEHHHHCCEEE
CSTIQFDFNLPERFDLTYVGEDNKKHRPYIIHRALLGSIERFTGVLIEHYAGHLPIWLSP
EEEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
IQAAIIPIADRHLDYAKEVFELLKVQNIRTYIDDRPERMNAKIRDNELQKIPILLVVGDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEEEECCH
EVQEKTFSVRSKNEEFQGVMGIYDFIEKLKTAIQNKR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKIVFNNQEYDLQAGIVGIELLKALNLDSKDIIGISIDGIIYDLQTPIYHGGTLKPITKQ
CEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCCEECCCCCCCCCCC
DKESLDILRHSLAHIMAQALKEIYGDEKVHLGIGPTTEDGFFYDVEVEGHHIGEEDLKII
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEEECCCCCCHHHHHHH
ENKMKEIIKRGLDIKRKELPREEALKFFENKKEVYKLDIINSLPSDAKISVYEQGEFVDL
HHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCEEEEEEHHCCCCCCEEEEEECCCCHHH
CRGPHIPNTALAGAFKLSSIAGAYWRADASKPMLQRIYGIAFWTEKELEERLKLYEEAKK
HCCCCCCCHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHEECHHHHHHHHHHHHHHHH
RDHRRLGKELELFTIDEQVGPGLIIWLPKGAILRQVVEDYLKEKHRALNYQFVYTPHVGK
HHHHHCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
STLWETSGHLSYYKENMFPGMKIEEESYYVKPMNCPFHMAIYKSKIRSHKELPIKIAELG
CCCCCCCCCHHHHHHHCCCCCEEECCCEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCC
TVYRYEMSGVLHGLMRVRGFTQDDAHIFCKEEDVKSVIKETLELAFDVLKDFGFYDYQIF
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
ISTKPKDAIGTEENWKISESALKESLEALDKTYEIDEGGGAFYGPKIDIKIKDAIGRFWQ
EECCCCCCCCCCCCCEECHHHHHHHHHHHHHHEEECCCCCEEECCEEEEEEHHHHCCEEE
CSTIQFDFNLPERFDLTYVGEDNKKHRPYIIHRALLGSIERFTGVLIEHYAGHLPIWLSP
EEEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
IQAAIIPIADRHLDYAKEVFELLKVQNIRTYIDDRPERMNAKIRDNELQKIPILLVVGDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEEEECCH
EVQEKTFSVRSKNEEFQGVMGIYDFIEKLKTAIQNKR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]