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The map label for this gene is ilvD

Identifier: 195952597

GI number: 195952597

Start: 196839

End: 198503

Strand: Direct

Name: ilvD

Synonym: HY04AAS1_0217

Alternate gene names: 195952597

Gene position: 196839-198503 (Clockwise)

Preceding gene: 195952596

Following gene: 195952598

Centisome position: 12.62

GC content: 40.06

Gene sequence:

>1665_bases
ATGAGAAGCGATATAATACAAAAAGGCTATGACAAAGCCCCGCATAGATCTTTATTGAGGGCTTGTGGTTTTAAAGATGA
GGATTTTGGAAAACCCATCATAGGTGTTGCTAATTCTTATATAGATATTGTGCCTGGGCATGTGCATCTTAGAGAATTTG
TAGAACCTATAAAAGAAGAGATTAGAAAAGCCGGTGCTATACCAGTAGAGTTTAACACCATAGGTGTAGATGACGGTATA
GCTATGGGACATTACGGTATGCACTACTCTTTACCTAGTAGAGAACTTATAGCAGATGCTATAGAAACCGTTGTAGAAGC
TCATCAGCTTGATGGTCTTATCTGCATACCAAACTGCGATAAAATAGTCCCCGGTATGCTAATGGGTGCTCTTAGAGTAA
ACGTGCCTACGGTGTTTATAAGCGGCGGTCCCATGGCTGCTGGTAAAATAGGAGATAAAAAAGTAGATCTTATATCTGTT
TTTGAAGGGGTTGGCAAGCTAAACAAAGGTGAGATAACAGAAAAAGATCTTCTTGTTATAGAGCAAAACGCTTGTCCTTC
TTGCGGTTCTTGTTCTGGGCTTTTTACGGCAAACTCTATGAACTGCCTTACTGAAGTGCTTGGTCTTGGACTTCCAGGAA
ACGGTACCACCTTAGCCATAGACCCAAGAAGAGAAATGCTTGCAAGGCAAGCTGCTAGACAAGTAGTAGAACTTGTAAAG
ATAGATTTAAAACCAAGGGATATAGTTACAAAAGCATCTTTTGACAATGCTTTTAGAGTTGATATAGCTATGGGTGGATC
TTCCAACACAGTGCTTCATCTTTTGGCTATTGCAAGAGAGGCTGGTATAGAGTACAAAATGGAAGATATAGATAAAATTT
CAAGAGAAACACCTACGCTTTGCAAAATATCCCCTTCTTCAGATTATCATATGGATGACCTTGATAGAGCTGGTGGAATA
AGTGCTATCATGAAAGAACTTCTTAGAAATGGTTTATTTGACGGTAAACAAAGAACGGTGACTACAAAAACCATAGAAGA
GATCGTAAAAGATGTAGAAATAATGGATGAAAACGTCATAAGACGTATAGACAACGCTTATTCAAAAGACGGTGGTCTTG
CCATACTATTTGGTAATTTGGCACCCAAAGGAAGCGTGGTAAAAACTGCTGGTGTTGCCAAAGAGATGCTGCAGTTCAAA
GGGAAAGCCATATGTTTTGATTCCGAAGAAGAAGCCATAGAAGGTATAAGGGGTGGAAAGGTAAAACCCGGCCATGTAGT
GGTCATAAGATACGAAGGTCCAAAAGGTGGTCCTGGTATGAGGGAGATGCTAAGCCCCACTTCCACCATAATGGGTATGG
GTCTTGGAAGTTCTGTGGCCCTTATTACAGATGGAAGGTTTTCAGGTGGTACTAGAGGTGCTTGTATAGGGCATATATCT
CCAGAAGCAGCAGCTGGTGGCCCTATTGGTATAGTTCAAGACGGTGATGAAATACTTATAGACATACCAAATAGAAAGCT
TGAGCTTTTGATATCTCAAGAAGAGTTTGATGCTCGTTTAAAAGCTTTTAAACCAAAGGAAAAACTTATTAAAAGCTCAT
GGCTCAAGCGTTATAGGAAATTTGTAAAAGATGCTTCAGAGGGTGCTATTTTATCTGCGGATTGA

Upstream 100 bases:

>100_bases
GGCTAGTAGACTTATCAAACAAGCTATGGAAAAGTTCCCAGAGGATTTTGCCAAGAAAGAACAAAAATCCTTTGTAAATA
TTTAAAGTTTGGAGGTTTTT

Downstream 100 bases:

>100_bases
CATTTAGTTGATGTTTAAGATAGAAAAAACCAATGGTTTAGCAAGGGCTGGTATATTATCAACTTTACATGGTGATATAT
TAACACCTGTTTTTATGCCG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MRSDIIQKGYDKAPHRSLLRACGFKDEDFGKPIIGVANSYIDIVPGHVHLREFVEPIKEEIRKAGAIPVEFNTIGVDDGI
AMGHYGMHYSLPSRELIADAIETVVEAHQLDGLICIPNCDKIVPGMLMGALRVNVPTVFISGGPMAAGKIGDKKVDLISV
FEGVGKLNKGEITEKDLLVIEQNACPSCGSCSGLFTANSMNCLTEVLGLGLPGNGTTLAIDPRREMLARQAARQVVELVK
IDLKPRDIVTKASFDNAFRVDIAMGGSSNTVLHLLAIAREAGIEYKMEDIDKISRETPTLCKISPSSDYHMDDLDRAGGI
SAIMKELLRNGLFDGKQRTVTTKTIEEIVKDVEIMDENVIRRIDNAYSKDGGLAILFGNLAPKGSVVKTAGVAKEMLQFK
GKAICFDSEEEAIEGIRGGKVKPGHVVVIRYEGPKGGPGMREMLSPTSTIMGMGLGSSVALITDGRFSGGTRGACIGHIS
PEAAAGGPIGIVQDGDEILIDIPNRKLELLISQEEFDARLKAFKPKEKLIKSSWLKRYRKFVKDASEGAILSAD

Sequences:

>Translated_554_residues
MRSDIIQKGYDKAPHRSLLRACGFKDEDFGKPIIGVANSYIDIVPGHVHLREFVEPIKEEIRKAGAIPVEFNTIGVDDGI
AMGHYGMHYSLPSRELIADAIETVVEAHQLDGLICIPNCDKIVPGMLMGALRVNVPTVFISGGPMAAGKIGDKKVDLISV
FEGVGKLNKGEITEKDLLVIEQNACPSCGSCSGLFTANSMNCLTEVLGLGLPGNGTTLAIDPRREMLARQAARQVVELVK
IDLKPRDIVTKASFDNAFRVDIAMGGSSNTVLHLLAIAREAGIEYKMEDIDKISRETPTLCKISPSSDYHMDDLDRAGGI
SAIMKELLRNGLFDGKQRTVTTKTIEEIVKDVEIMDENVIRRIDNAYSKDGGLAILFGNLAPKGSVVKTAGVAKEMLQFK
GKAICFDSEEEAIEGIRGGKVKPGHVVVIRYEGPKGGPGMREMLSPTSTIMGMGLGSSVALITDGRFSGGTRGACIGHIS
PEAAAGGPIGIVQDGDEILIDIPNRKLELLISQEEFDARLKAFKPKEKLIKSSWLKRYRKFVKDASEGAILSAD
>Mature_554_residues
MRSDIIQKGYDKAPHRSLLRACGFKDEDFGKPIIGVANSYIDIVPGHVHLREFVEPIKEEIRKAGAIPVEFNTIGVDDGI
AMGHYGMHYSLPSRELIADAIETVVEAHQLDGLICIPNCDKIVPGMLMGALRVNVPTVFISGGPMAAGKIGDKKVDLISV
FEGVGKLNKGEITEKDLLVIEQNACPSCGSCSGLFTANSMNCLTEVLGLGLPGNGTTLAIDPRREMLARQAARQVVELVK
IDLKPRDIVTKASFDNAFRVDIAMGGSSNTVLHLLAIAREAGIEYKMEDIDKISRETPTLCKISPSSDYHMDDLDRAGGI
SAIMKELLRNGLFDGKQRTVTTKTIEEIVKDVEIMDENVIRRIDNAYSKDGGLAILFGNLAPKGSVVKTAGVAKEMLQFK
GKAICFDSEEEAIEGIRGGKVKPGHVVVIRYEGPKGGPGMREMLSPTSTIMGMGLGSSVALITDGRFSGGTRGACIGHIS
PEAAAGGPIGIVQDGDEILIDIPNRKLELLISQEEFDARLKAFKPKEKLIKSSWLKRYRKFVKDASEGAILSAD

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=606, Percent_Identity=49.5049504950495, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1788157, Length=531, Percent_Identity=29.9435028248588, Blast_Score=222, Evalue=5e-59,
Organism=Escherichia coli, GI2367371, Length=530, Percent_Identity=31.6981132075472, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1786464, Length=491, Percent_Identity=30.9572301425662, Blast_Score=186, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6322476, Length=561, Percent_Identity=41.3547237076649, Blast_Score=428, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_HYDS0 (B4U6Z8)

Other databases:

- EMBL:   CP001130
- RefSeq:   YP_002120887.1
- GeneID:   6743006
- GenomeReviews:   CP001130_GR
- KEGG:   hya:HY04AAS1_0217
- HOGENOM:   HBG671001
- OMA:   KVPCLSK
- ProtClustDB:   PRK00911
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59772; Mature: 59772

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSDIIQKGYDKAPHRSLLRACGFKDEDFGKPIIGVANSYIDIVPGHVHLREFVEPIKEE
CCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCEEEECCCHHHHHHHHHHHHHH
IRKAGAIPVEFNTIGVDDGIAMGHYGMHYSLPSRELIADAIETVVEAHQLDGLICIPNCD
HHHCCCCEEEEEEECCCCCCEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCC
KIVPGMLMGALRVNVPTVFISGGPMAAGKIGDKKVDLISVFEGVGKLNKGEITEKDLLVI
HHHHHHHHHHHEECCCEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEE
EQNACPSCGSCSGLFTANSMNCLTEVLGLGLPGNGTTLAIDPRREMLARQAARQVVELVK
ECCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHH
IDLKPRDIVTKASFDNAFRVDIAMGGSSNTVLHLLAIAREAGIEYKMEDIDKISRETPTL
CCCCCHHEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCE
CKISPSSDYHMDDLDRAGGISAIMKELLRNGLFDGKQRTVTTKTIEEIVKDVEIMDENVI
EEECCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
RRIDNAYSKDGGLAILFGNLAPKGSVVKTAGVAKEMLQFKGKAICFDSEEEAIEGIRGGK
HHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHCCCEEEECCHHHHHHCCCCCC
VKPGHVVVIRYEGPKGGPGMREMLSPTSTIMGMGLGSSVALITDGRFSGGTRGACIGHIS
CCCCEEEEEEECCCCCCCCHHHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCC
PEAAAGGPIGIVQDGDEILIDIPNRKLELLISQEEFDARLKAFKPKEKLIKSSWLKRYRK
CCCCCCCCEEEEECCCEEEEECCCCEEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FVKDASEGAILSAD
HHHCCCCCCEEECC
>Mature Secondary Structure
MRSDIIQKGYDKAPHRSLLRACGFKDEDFGKPIIGVANSYIDIVPGHVHLREFVEPIKEE
CCCHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCEEEECCCHHHHHHHHHHHHHH
IRKAGAIPVEFNTIGVDDGIAMGHYGMHYSLPSRELIADAIETVVEAHQLDGLICIPNCD
HHHCCCCEEEEEEECCCCCCEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCEEECCCCC
KIVPGMLMGALRVNVPTVFISGGPMAAGKIGDKKVDLISVFEGVGKLNKGEITEKDLLVI
HHHHHHHHHHHEECCCEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEE
EQNACPSCGSCSGLFTANSMNCLTEVLGLGLPGNGTTLAIDPRREMLARQAARQVVELVK
ECCCCCCCCCCCCCEECCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHH
IDLKPRDIVTKASFDNAFRVDIAMGGSSNTVLHLLAIAREAGIEYKMEDIDKISRETPTL
CCCCCHHEEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCE
CKISPSSDYHMDDLDRAGGISAIMKELLRNGLFDGKQRTVTTKTIEEIVKDVEIMDENVI
EEECCCCCCCCCHHHHCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHH
RRIDNAYSKDGGLAILFGNLAPKGSVVKTAGVAKEMLQFKGKAICFDSEEEAIEGIRGGK
HHHHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHCCCEEEECCHHHHHHCCCCCC
VKPGHVVVIRYEGPKGGPGMREMLSPTSTIMGMGLGSSVALITDGRFSGGTRGACIGHIS
CCCCEEEEEEECCCCCCCCHHHHHCCHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCC
PEAAAGGPIGIVQDGDEILIDIPNRKLELLISQEEFDARLKAFKPKEKLIKSSWLKRYRK
CCCCCCCCEEEEECCCEEEEECCCCEEEEEECHHHHHHHHHHCCCHHHHHHHHHHHHHHH
FVKDASEGAILSAD
HHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA